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Citations for abcam :
1001 - 1050 of 1481 citations for 5 tert Butyl 3 isocyanatoisoxazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 0.5% sodium deoxycholate and protease inhibitor cocktail) and incubated with 5 μg anti-ZAP (Abcam, ab154680) antibody for 1 hour at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... magnetic protein A/G beads were prepared with 5 μg of rabbit anti-TIA1 (Abcam - #ab40693) or normal rabbit IgG antibodies (from Magna RIP kit) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... IHC staining for human leukocyte antigen (HLA) primary antibody (Abcam, clone EMR8-5, 1:50 dilution) and N-sulfated heparan sulfate (Amsbio ...
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bioRxiv - Cancer Biology 2022Quote: Nuclear extracts from 5*10^6 cells were collected using a Nuclear Extraction Kit (Abcam, ab113747) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were blocked in 5% non-fat milk and stained with an anti-H3 antibody (abcam #1791 ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with primary antibodies in 5% BSA TBS-T against SDHA (1:10000, Abcam; ab14715), MT-CO1 (1:10000 ...
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bioRxiv - Neuroscience 2022Quote: ... blots were incubated overnight with primary antibodies: anti-beta actin (1:2500 in 5% BSA, Abcam), anti-LC3B (1:1000 in 5% BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... Immunofluorescence staining was performed on 5 µm sagittal sections using rabbit anti-mouse CD31(Abcam, ab281583) and rat anti-mouse Emcn primary antibodies to label endothelial cells ...
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bioRxiv - Biophysics 2023Quote: ... cells were incubated with primary antibody (rabbit anti-alpha tubulin antibody, Abcam, ab18251, 5 μg/ml) for 1 hour at 20°C followed by three washing steps with PBS ...
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bioRxiv - Microbiology 2024Quote: ... and histone H3 were detected with anti-HA (Abcam 9110, 1:1,000 in 5% milk-PBST) and anti-H3 (Abcam ab1791 ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were blocked in 5% skimmed milk then incubated with the primary antibodies ACSL4 (AbCam, ab155282), Transferrin Receptor 1 (TFR1 ...
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Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: ... CXCL6 and CXCL8 were measured using ELISA (BioTechne for CXCL1, 5, 6, 8, Abcam for CXCL3). CCL26 ...
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bioRxiv - Immunology 2023Quote: ... sections were incubated for 1 hour at RT with D-PBS (-) + 5 % normal goat serum (Abcam) to block nonspecific binding sites and then incubated overnight at 4 °C with primary antibodies diluted in D-PBS (-) ...
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bioRxiv - Genetics 2023Quote: ... and blocked by overnight incubation in PBS + 0.1% Triton X-100 and 5% donkey (Abcam, cat.no.ab7475) or goat serum (DAKO ...
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bioRxiv - Cancer Biology 2023Quote: ... The antibodies were incubated with primary antibody anti-CXCR4 (1:1000 in 5% BSA) (Abcam, ab181020) or β-actin (1:10000 in 5% milk in TBST ...
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bioRxiv - Biochemistry 2023Quote: ... blocked in 5% normal goat serum and then incubated with anti-mouse GLUT1 (Abcam, ab40084,1:200) at 4°C overnight ...
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bioRxiv - Immunology 2024Quote: ... followed by overnight staining with anti-pT73 Rab10 antibody (clone MJF-R21-22-5, AbCam ab241060) diluted 1:500 in 1x Permeabilisation buffer with 5% Normal Donkey Serum at +40C and 1 h staining with DyLightTM 649 Donkey anti-rabbit IgG (BioLegend ...
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bioRxiv - Physiology 2024Quote: Triglyceride content was determined in 3T3L1 lysates (5% NP-40) using a calorimetric kit (Ab65336, Abcam) following the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... ELISA kits (IL-1β, IL-4, IL-5, IL-6) were purchased from Abcam (Cambridge, UK). BCA protein quantification kits were purchased from Thermo fisher scientific (Waltham ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... fragmented DNA was incubated overnight with 5 μg of anti-ERα (Abcam Cat# ab32063, RRID: AB_732249), or anti-HIF2α (Novus Cat# NB100-122 ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:33 in PBS 1X supplemented with 3% BSA and with polyclonal rabbit anti-CD31 (cat. Ab28364, Abcam, Cambridge, UK) diluted 1:50 in the same blocking buffer ...
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bioRxiv - Genetics 2021Quote: ... were incubated in blocking solution (0.5% bovine serum albumin in PBS) with 3 μg of antibody to histone-H3 K27ac (Abcam, cat. # ab4729) for 3 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Sonicated DNA was pre-cleared using Protein A magnetic beads followed by overnight chromatin immunoprecipitations with 3 ug H3K27Ac (Abcam ab4729), 5 ug FoxP3 (Abcam ab150743) ...
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bioRxiv - Zoology 2020Quote: ... 0-3 days old) and older buds (stage 3, 1 month old) by two different means: anti Phospho-histone H3 (PHH3) immunolocalization (Abcam ab5176) antibody (1:200 ...
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bioRxiv - Immunology 2021Quote: ... Slides were then washed 3×10 minutes in PBS at RT and again blocked with 10% normal donkey serum (Abcam, ab7475) for 20 minutes at room temperature before being incubated with PBS containing WGA-555 (Thermo Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... In the next step the cells were washed with 3% BSA solution and incubated with 1x Phalloidin-iFluor594 (Abcam, stock 1000x) solution (in 1% BSA ...
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bioRxiv - Neuroscience 2021Quote: ... The the membranes were incubated overnight at 4°C with rabbit anti-VEGFR-3 antibodies (Abcam, Cambridge, MA, USA; 1:50). Horseradish peroxidase (HRP)-conjugated secondary goat anti-rabbit antibodies (Sigma-Aldrich Co. ...
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bioRxiv - Cancer Biology 2020Quote: Beads that were incubated with FLI 7.3 mouse antibody were resuspended in 100 µL Dig-wash buffer and incubated with rabbit anti-mouse secondary antibody (Abcam, ab46540) at a dilution of 1:100 on a rotator for 1 hour at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated at RT for 2 h with mouse anti-CTLA-4 monoclonal antibody (diluted: 1:3 000; Abcam, Cambridge, UK) as the primary antibody ...
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bioRxiv - Neuroscience 2020Quote: ... BLA slices were incubated overnight at 4°C in a solution containing 0.1% Triton X and 3% normal goat serum in PBS and chicken anti-GFP (1:1000, Abcam, ab13970, polyclonal). They were then rinsed in PBS (3 × 10min ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% SDS) and incubated overnight at 4°C with 3 μL specific mouse monoclonal antibody recognizing Bcl11a (Abcam Cat# ab19487, RRID:AB_444947) or unspecific IgG1 antibody (Cell Signaling Technology Cat# 5415 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed in PBS (3×10 min) and incubated for 2h in secondary antibody diluted in blocking solution (1:1000 Goat-α-mouse AlexaFluor488, abcam ab150113 ...
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bioRxiv - Immunology 2020Quote: ... Sections were incubated overnight at 4°C with a rabbit polyclonal anti-mouse blocking antibody (MUC2, ab90007, Abcam Ltd; Occludin, ab168986, Abcam Ltd; caspase 3, ab44976, Abcam Ltd). The sections were washed with PBS and incubated with a secondary antibody for 30 min ...
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bioRxiv - Immunology 2021Quote: ... 100 μL of cells expressing mHVEM-mCherry proteins were mixed with 0.3 μg mBTLA- penta-His-tag/mCD160-biotin proteins and 0.5 μg of green fluorescent anti-His-tag (Abcam; Cat: ab1206)/Alexa Fluor 488 conjugated streptavidin (Life technologies ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... Non-specific sites on the membrane were blocked using 3% BSA and further incubated with anti-SATB2 antibody (1:1000 abcam ab34735) in 0.3% BSA overnight at 4°C with constant rocking ...
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bioRxiv - Cell Biology 2020Quote: ... the ovarian samples were incubated overnight at 4°C with primary antibodies against caspase-3 (1:1000 dilution, Abcam, Cambridge, MA) and NLRP3 (1:1000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing goat anti-rabbit IgG H&L Alexa Fluor 488 (abcam; ab150077).
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bioRxiv - Cell Biology 2022Quote: ... log-phase liquid cultures were divided in two and incubated for different times in YES-Glucose medium treated with 10 µM 3-BrB-PP1 (Abcam, ab143756), or in medium lacking the analog kinase inhibitor ...
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bioRxiv - Cell Biology 2024Quote: ... Paraffin embedded liver sections (3 μm) from human and mouse liver samples were stained for aSMA (1:100, Abcam, Cat#ab5694), KI67 (1:50 ...
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bioRxiv - Immunology 2024Quote: ... sections were repaired using EDTA antigen recovery buffer and incubated overnight at 4 °C with anti-dengue virus 1+2+3+4 primary antibody (Abcam, ab26837). Next ...
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bioRxiv - Biophysics 2024Quote: ... The micro-tissues were then permeabilized in 0.5% Triton X-100 in PBS for 5 min and blocked in 3% bovine serum albumin (BSA) in PBS before they were incubated with primary antibodies (anti-sarcomeric alpha actinin (Abcam #9465) or desmin (Cell Signaling #5332) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in 47 µl of Inside Perm Solution and stained with 3 µl of Anti-PBR antibody [EPR5384] (abcam, ab199836). After 10 minutes of incubation ...
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bioRxiv - Immunology 2023Quote: ... The sections were incubated overnight with anti-cell marker cluster of differentiation (CD) 3 antibody (1:200, Abcam, ab16669, Cambridge, UK) at 4°C in a humidified incubation chamber ...
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bioRxiv - Neuroscience 2022Quote: ... Slices containing VPMpc were incubated overnight at 4°C in a solution containing 0.1% Triton X and 3% normal goat serum in PBS and chicken anti-GFP (1:1000, Abcam, ab13970, polyclonal). They were then rinsed in PBS (3 × 10min ...
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bioRxiv - Cancer Biology 2023Quote: ... was performed on sectioned paraffin-embedded tumor constructs or fixed cells cultured for 3 to 7 days on X-tra® slides to analyze biomarker presence of SF-1 (ab240394, Abcam), Melan A (ab210546 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with a rabbit anti-Kv4.3 primary antibody (1:10000, Alomone Labs) together with chicken (G)/mouse (L) anti-TH primary antibody (1:1000, Abcam / Merck) in a carrier solution (1% NGS ...
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bioRxiv - Biochemistry 2022Quote: Cells were incubated in complete media in the presence of 100 nM bafilomycin A1 (to block the lysosomal turnover of LC3-II) for 3 hrs and subjected to immunoblotting using the mCherry antibody (Abcam, ab125096) with a ratio of 1:1000 ...
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bioRxiv - Biophysics 2023Quote: ... Wells were rinsed with PBS and incubated overnight with 50 μg/mL anti-human CD45RA antibody (Abcam ab212774, lot GR3365431-3), at 4°C.
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bioRxiv - Cancer Biology 2023Quote: ... cells were blocked in 3% -BSA/1x PBS for 30 min prior to incubation with fluorescence-conjugated Phalloidin (abcam, Cat# ab176753) at 1:000 for 1 hr at room temperature ...
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bioRxiv - Immunology 2023Quote: ... was incubated at 40C overnight followed by application of a rabbit-specific HRP/3-amino-9-ethylcarbazole (AEC) IHC Detection Kit - Micro-polymer (abcam, ab236468) incubated at room temperature for 1 hr ...