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Citations for abcam :
1101 - 1150 of 1481 citations for 5 tert Butyl 3 isocyanatoisoxazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Cells/Organoids were treated with the following compounds as indicated: 5FU ( Abcam, ab142387-5g, 2-5 µM), Metformin (Cayman Chemicals ...
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bioRxiv - Cell Biology 2024Quote: ... for 15 min at RT and blocked with blocking buffer (5% normal donkey serum (NDS) (Abcam, ab7475) + 1% bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2021Quote: ... Non-decalcified E16.5 and P0 samples had mineralized bone visualized by von Kossa staining (n=3 per age and genotype) with Nuclear Fast Red counterstaining as part of an established protocol (Abcam, Cambridge, UK). For osteoclast staining ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then incubated for 60 min at room temperature with primary antibody dilutions prepared in 3 % BSA in PBS as follows: mouse anti-Bub1 (ab54893, Abcam, 1:500); mouse anti-Mad2 (sc-65492 ...
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bioRxiv - Bioengineering 2020Quote: ... Permeabilized cells were rinsed 3 times with 1X PBS and incubated in 1X PBS with primary antibody Rabbit-α-Tomm20 (Abcam, ab186735) and Mouse-α-LaminA/C (Abcam ...
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bioRxiv - Physiology 2020Quote: ... Cells were washed 3 × in TBS and incubated overnight (4°C) in 300 μl of anti-desmin (Abcam Cat# ab15200, RRID: AB_301744) primary antibody made up in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... the samples were incubated for 3 h at room temperature with the goat anti-chicken antibody conjugated with Alexa Fluor® 488 (Abcam 150169) at a concentration of 1:500 in PBS-blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... every fourth section throughout the hippocampus/dentate gyrus was collected and stained with panel 3 = anti-mouse BrdU (ab8152, Abcam, 1:500), anti-mouse doublecortin (DCX ...
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bioRxiv - Cell Biology 2022Quote: ... The cultures subsequently underwent 3 cycles of 10 min PBS washes and incubated for 40 min with the appropriate secondary antibodies (Abcam or Jackson) and DAPI ...
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bioRxiv - Cell Biology 2022Quote: The following primary antibodies (all diluted in 3% BSA in PBS) were used at the final concentration indicated: chicken anti-GFP (ab13970 from Abcam, 1:5000), rabbit anti-mCherry (GTX128508 from Genetex ...
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bioRxiv - Molecular Biology 2022Quote: ... NAD(P)H quinone dehydrogenase 1 (NQO-1; #ab80588) and pan-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; #ab8245) antibodies were provided by Abcam (Cambridge, England). The p38 MAPK inhibitor SB203580 (#S8307 ...
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bioRxiv - Microbiology 2020Quote: ... 1:300 dilution of mouse anti-PfDHODH #3 (a kind gift from Margeret Phillips, University of Texas Southwestern) or 1/5000 mouse anti-PfAldolase-HRP (Abcam, Cat #ab38905). The next day ...
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bioRxiv - Immunology 2020Quote: ... T cells were cultured with 2-deoxy-2-((7-nitro-2, 1, 3-benzooxadiazol-4-yl)amino)-glucose (2-NBDG) (Abcam 50μM, 1h), 4 ...
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bioRxiv - Immunology 2022Quote: ... albicans hyphae for 3 hours and then labelled with anti-histone H3 citrulline R2+R8+R17 (Abcam, Cambridge, MA; 0.014 mg/ml) followed by donkey anti-rabbit IgG Cy3 (Jackson Immunoresearch ...
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bioRxiv - Microbiology 2021Quote: ... Consecutive sections were processed for immunohistochemical staining with reovirus-specific rabbit polyclonal antiserum (1:1000) or activated caspase-3 (Abcam, 1:100). Images were taken on an EVOS FL Auto 2 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed 3 times in PB and were mounted on a glass slide in the mounting medium with DAPI (Abcam ab 104139). The slides were imaged at 20X on a Zeiss LSM 880 airyscan confocal microscope.
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bioRxiv - Physiology 2021Quote: ... Next day the sections were washed 3 × 10 minutes with PBS and stained with an anti-rabbit secondary antibody (1:1000, AB150077, Abcam, Alexa 488) in 2% BSA in PBS for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... The pellet was re-suspended with 0.9% of sodium chloride solution and reacted with a rabbit anti-mouse HMGB1 primary antibody for 3 hrs at room temperature (1:350, abcam, Cat. #ab18256), followed by a goat anti-rabbit secondary antibody conjugated with Cy3 for 1hr at room temperature (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: ... 100-200 μg of precleared chromatin was incubated at 4°C for three hours with magnetic beads that had been prebound to one of the following antibodies: 3 μg of α-V5 antibody (Abcam ab9116), 1.5 μg of α-H3K9me2 antibody (Abcam ab1220) ...
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bioRxiv - Physiology 2022Quote: ... washed with PBS-T 3 times for 10min and incubated O/N at 4°C with primary antibody (Rabbit anti-Calnexin, Abcam, Cat# ab133615) at a dilution of 1:1000 in PBS-T ...
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bioRxiv - Neuroscience 2022Quote: ... samples were blocked in PBTx (1% Triton X-100 in PBS) containing 3% bovine serum albumin and incubated over three nights with chicken anti-GFP antibody (Abcam Cat#ab13790). After several washes in PBTx ...
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bioRxiv - Molecular Biology 2023Quote: ... The primary antibodies were diluted in 3% BSA/PBS and incubated overnight at 4°C: rabbit anti-RPA34 (Abcam ab97594, 1:1000), mouse anti-PML (Santa Cruz Biotechnology sc-966 ...
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bioRxiv - Microbiology 2023Quote: ... bound complexes were isolated by overnight precipitation of the pre-cleared chromatin with 100 µL of blocked beads in combination with 3-4 µg of antibodies (anti-Histone H3 (Abcam ab17913, µg), Histone H3Ac (Millipore 17-615 ...
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bioRxiv - Neuroscience 2023Quote: ... Brain slices containing locus coeruleus and NTS were incubated in primary antibody (1:500 dilution of 3% normal goat serum in 1% Triton X100 dissolved in PBS, rabbit anti-mCherry IgG, Abcam catalog # ab167453) for 24-48 hours at 4°C ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... the sections were washed 3 more times in PBS followed by incubation in the secondary antibody (antirabbit Alexa Fluor-488, Abcam; Sydney, Australia) diluted in blocking solution (1:250 ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were then incubated overnight at 4°C with the following primary antibodies diluted in PBS 3% BSA 0.2% Triton X100: anti-β-Tubulin (clone 2A1A9, dilution 1/200, Abcam, Cambridge, UK), anti-YAP1 (clone D8H1X ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cells were washed 3 times with 1X PBST before adding anti-Rabbit secondary antibody (Catalog No: ab150080, Abcam, Waltham, MA, USA) for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... The sections were blocked with 10% goat serum/3% BSA/0.3% Triton X-100 and stained with antibodies to Netrin-1 (Abcam #ab126729, 1:200), Cytokeratin (human specific cytokeratin 7 and 8 ...
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bioRxiv - Immunology 2024Quote: ... The resulting lysates was quantified using cytokine specific ELISA kits (IL-18 Thermo Fisher #BMS618-3 and IL-18 BP Abcam # ab254509), and absorbance was measured on the Tecan Spark 10M (Tecan ...
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bioRxiv - Neuroscience 2024Quote: ... as reported.9 Tissue sections were then permeabilized with 0.3% Triton X-100 for 10 min at RT and incubated with rabbit polyclonal anti-PSD95 (1:250, ab18258, Abcam, Cambridge, UK) overnight at 4°C ...
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bioRxiv - Pathology 2024Quote: ... The sections were blocked with PBS / 3% BSA for 30min and sequentially immunostained with the primary rabbit anti-α-SMA antibody (Abcam, #ab5694) and the corresponding secondary HRP-conjugated goat anti-rabbit IgG antibody (Genetech ...
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bioRxiv - Immunology 2024Quote: ... before blocking with Blocking buffer (PBS with 10% normal donkey serum and 3% BSA) followed by overnight incubation with rabbit antimouse CD4 (abcam, Cat# ab183685) and rat antimouse Ly-6G (clone 1A8 ...
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bioRxiv - Cell Biology 2024Quote: ... primary antibody incubation was performed with DNA/RNA damage antibody (8-OHdG; Novus, Littleton, CO, USA) or cleaved caspase-3 (Abcam, Cambridge, UK) (both at 5 µg/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... then activation was induced by transfer of oocytes to R1EMC containing 150 µM Butyrolactone I (Affiniti R.P.L, Mamhead, UK) and 5 µM Cytochalasin B (Abcam) for 2 hours ...
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bioRxiv - Bioengineering 2021Quote: ... 5 μm thickness paraffin sections were stained with Alcian blue using manufacturers protocol (Alcian Blue Stain Kit, Abcam) and imaged via bright field microscopy with 10× and 40× objectives.
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bioRxiv - Cancer Biology 2021Quote: ... the digested cross-linked chromatin (10 μg) was subjected to immunoprecipitation with 5 μg of anti-H3K27me3 (Abcam), anti-H3K4me3 (Abcam) ...
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bioRxiv - Cancer Biology 2020Quote: ... and immunoprecipitation was carried out by adding 5-10 μg of antibody (α-RNA Pol II #ab817 abcam, α-cMYC #13987 ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were permeabilized in cold methanol (−20 C) and blocked with 5% normal goat serum (Abcam ab7481) with 0.05% sodium azide (Life Technologies 50062Z) ...
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bioRxiv - Cancer Biology 2022Quote: ... Directly conjugated primary antibodies were applied last after blocking with 5% normal rabbit and/or goat sera (Abcam). Cell nuclei were visualized with Hoechst (Biotium ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues were then incubated in blocking buffer 1 (BB1) consisting of 5% v/v donkey serum (abcam, ab7475), 1% v/v BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... in a Tris-glycine-methanol transfer buffer overnight and detected with the following antibodies: 1:5000 monoclonal mouse anti-Hsp70 [C92F3A-5] (Abcam Cat# ab47455, RRID:AB_881520) (abcam), 1:15000 monoclonal mouse anti-Pgk1 (Thermo Fisher Scientific Cat# 459250 ...
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bioRxiv - Physiology 2020Quote: ... was interrogated using the JC-1 fluorophore (5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzimi-dazolylcarbocyanine iodide; 5 μM, 30 minutes, 37□C) (Abcam, Cambridge, UK), MitoTracker Red CMXRos (5 μM ...
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bioRxiv - Pathology 2021Quote: ... Deparaffinized and rehydrated 5-μm FFPE sections were subjected to antigen heat retrieval using citrate buffer (abcam, ab208572), in a pre-heated steam bath (100°C ...
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bioRxiv - Microbiology 2021Quote: ... Cells were blocked and permeabilized for 30min in PBS containing 0.1% TritonX and 5% Normal Donkey Serum (Abcam), stained overnight with mouse anti-G3BP (BD ...
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bioRxiv - Neuroscience 2021Quote: ... and then blocking solution (PBS supplemented with 2% Bovine Serum Albumin (Roth) and 5% Normal Goat Serum (Abcam)) was applied for 1 hour at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... Primary antibodies were diluted in 5% milk TBST and incubated with blots overnight at 4 °C (FGF1 – Abcam, ab207321 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with a primary antibody against serotonin (5-HT; Abcam, ab66047; in PBS, 1:10) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: For recordings, slices were superfused with oxygenated aCSF (∼2 ml/min, ∼33°C) containing Gabazine (5 µM; Abcam). A bipolar stimulating electrode was placed in the middle of visible barrel fields in layer 4 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary astrocyte cultures were loaded with the 5 µM chemical calcium indicator Rhod-2 AM (Abcam, Cat.: #ab142780) in standard artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Cancer Biology 2024Quote: ... wells were washed twice with 100 µL of PBS and then blocked with 5% goat serum (Abcam, #ab7481) and 1 mg/mL of BSA (GeminiBio ...