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Citations for abcam :
801 - 850 of 854 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Slides containing 4 sections per slide and 8 sections per sample were incubated with primary antibodies at 4°C overnight and then washed three times with PBS before incubating them with goat anti-mouse IgG (H&L) secondary antibody (Alexa Fluor® 488 in 1% BSA PBST, 1:300; Abcam #ab150113) for 1 h at room temperature in the dark ...
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bioRxiv - Synthetic Biology 2022Quote: ... 0.1% Tween 20) and subsequently probed for 1 h with one of the following: horseradish peroxidase (HRP)-conjugated anti-DDDDK antibody (Abcam, cat # ab49763) that recognized the FLAG epitope tag ...
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bioRxiv - Cell Biology 2023Quote: ... cells were stained for 30 min with polyclonal fluorescein-isothiocyanate (FITC)-conjugated goat anti-mouse IgG H&L specific antibody (Abcam, United Kingdom) diluted 1:50 in RS-BSA 0.1% ...
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bioRxiv - Bioengineering 2023Quote: ... and stained with either H&E or specific immunohistochemistry markers (HA-Tag C29F4 #3724 from Cell Signaling Technology; GranzymeB Leica Biosystems PA0291; CD3 Abcam 16669 ...
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bioRxiv - Molecular Biology 2023Quote: ... and subsequently incubated for 1 h with a 1:5000 dilution of a horseradish peroxidase-conjugated goat anti-mouse secondary antibody (ab6789, Abcam, Cambridge, UK) or goat anti-rabbit secondary antibody (ab6721 ...
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bioRxiv - Plant Biology 2024Quote: ... using a solution containing goat polyclonal secondary antibody to mouse or rabbit IgG – H&L conjugated to Alexa Fluor 488 (Abcam AB150113, AB150077), added in a 1:100 dilution ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in antibody buffer (1%BSA in 1xPBS+0.02% Tween20) for 1 h at RT and stained overnight (o/n) with primary antibody anti-Tp63 (Abcam, clone 4A4, #ab735) diluted 1:1000 at 4°C ...
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bioRxiv - Pathology 2024Quote: ... Sections were then incubated with fluorochrome conjugated secondary antibody – goat anti-rabbit IgG H&L (Alexa Fluor® 488, ab150077, Abcam, UK) diluted in 1:500 ratio with PBS at room temperature for 30 min in the dark ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were washed three times with 1X PBS and then incubated with the secondary antibody (Goat Anti-Rabbit IgG H&L, Alexa Fluor® 647, Abcam, ab150079) at a 1:500 dilution in 2% BSA in 1X PBS for 1 hour at room temperature in the dark ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... (Epitope V, novoprotein, DA119), RSV Pre-F Antibody (3C12) (Epitope Φ, novoprotein, DA101) and Rabbit Anti-Human IgG H&L (FITC) (abcam, ab6755), and ELISA with Respiratory Syncytial Virus preF Antibody Pair (Φ & □ ...
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bioRxiv - Immunology 2024Quote: ... The membranes were washed and incubated with the respective HRP-coupled secondary antibodies for one hour at room temperature: goat anti-rabbit IgG H&L (#ab205718, 1:5000; abcam; RRID: AB_2819160), Goat Anti-Mouse IgG (H + L ...
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bioRxiv - Biophysics 2024Quote: ... and Alexa Fluor 488-labeled Goat Anti-Rabbit IgG (H+L) (Beyotime, Shanghai, China) and HaCaT cells were immune-stained with anti-pan cytokeratin mouse recombinant multiclonal antibody (Abcam, Cambridge, UK) and Alexa Fluor 647-labeled Goat Anti-Mouse IgG (H+L ...
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bioRxiv - Zoology 2024Quote: ... Samples were incubated with the primary antibody in 1% BSA in PBS overnight and then incubated during 1 hour at room temperature with a secondary antibody goat anti-mouse IgG H&L Alexa Fluor 488 (1:500; Abcam, Cat# ab150113) diluted in 1% BSA and then rinsed in PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Membranes were then blocked (5% milk in TBS-Tween buffer) and incubated for 18 h with primary antibodies: CD44 (Abcam, 1:1000 dilution); GFAP (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... Then the sections were blocked in 10% goat serum containing 1% BSA for 2 h at RT and sequentially incubated with primary antibodies: γ-H2AX (ab22551, Abcam; 1:100) or Ki67 (GTX16667 ...
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bioRxiv - Molecular Biology 2022Quote: ... membranes were incubated for 2 h in room temperature with the adequate HRP-conjugated secondary antibody: goat anti-rabbit IgG (1:5000, Abcam, GB; cat. 97051) or goat anti-mouse IgG (1:10000 ...
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bioRxiv - Neuroscience 2020Quote: ... This was followed by a 48h incubation in goat antirabbit IgG H&L (Alexa Fluor 555) antibody (1:500, Abcam, Abcam Cat# ab150078, RRID:AB_2722519) and then placed for 1 h in a Hoescht:PBS (1:10 000 ...
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bioRxiv - Neuroscience 2022Quote: ... for 1 h at room temperature and were incubated overnight at 4 °C with primary rabbit polyclonal antibodies against mouse MAGL (Abcam, Cambridge, UK; ab180016) or human MAGL (LSBio ...
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bioRxiv - Molecular Biology 2022Quote: ... in PBST for 1 h at room temperature and then immunoblotted overnight at 4 °C with corresponding primary antibodies: anti-FTO (Abcam, ab124892, 1/1000) or anti-β-Actin (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were blocked in 5% skim milk solution for 1 h at RT then incubated with primary antibody at 4 ℃ overnight: Anti-FMRP antibody (Abcam, ab17722, 1:1,000), anti-GAPDH antibody (Cell signaling technology ...
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bioRxiv - Systems Biology 2023Quote: ... the cells underwent an additional 30-minute blocking step using 1× blocking buffer and were then incubated for 1 hour with secondary antibodies – Goat Anti-Mouse IgG H&L (Alexa Fluor® 594, Abcam, Cambridge, UK), and Phalloidin-iFluor 488 Reagent (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... The brain sections of the rats with Gi-DREADDs were incubated first in a blocking solution (10% fetal horse serum and 0.15% Triton X-100 in PBS) for 1 h at 24 ± 1°C and then with primary rabbit anti-mCherry antibody (1:1000, Abcam, ab167453, Cambridge, UK) overnight at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The slides with cryosectioned tissue were washed with PBS followed by incubation in blocking solution (2% of BSA in PBS) for 1 h and then incubated overnight at 4°C with an anti-DBI antibody (rabbit, 1:1000, Abcam, Cat. No: ab231910). Sections were then incubated for 1 h at room temperature with anti-rabbit Alex Fluor 546 (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... secondary blotting was carried out in the same buffer with donkey anti-mouse IgG H+L Alexa Fluor 488 at a dilution of 1:2000 and donkey anti-rabbit IgG H+L Alexa Fluor 647 at a dilution of 1:5000 (Catalog #150079, Abcam, PLC, Cambridge, UK). Membranes were imaged using a GE Typhoon FLA9500 biomolecular imager in fluorescence mode ...
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bioRxiv - Biochemistry 2023Quote: ... for 10 min and blocked in 0.5% BSA in 0.5% Tx-PBS for 1 h at room temperature before incubation with mouse monoclonal anti-ψ-tubulin antibody (Abcam, ab11316, 1:1000) in 0.5% BSA in 0.5% Tx-PBS overnight at 4 ºC ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Secondary antibody incubation was done for 1 h at RT with the following horseradish peroxidase (HRP)-coupled antibodies: HRP-conjugated goat anti-mouse IgG (Abcam Cat# ab6789, RRID:AB_955439), HRP-conjugated goat anti-rabbit IgG (Abcam Cat# ab6721 ...
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bioRxiv - Developmental Biology 2022Quote: ... the sections were blocked with 1 % BSA in PBS for 1 h at room temperature and incubated with rabbit anti-PDGFR-β (1:200, Abcam, Cambridge, England) overnight ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for ~3 hours in secondary antibody solution containing donkey anti-rabbit IgG H&L Texas Red (abcam, Cambridge, UK; ab7081) and donkey anti-goat IgG H&L Alexa Fluor 488 (abcam ...
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bioRxiv - Neuroscience 2023Quote: ... The membranes were blocked with 5% non-fat milk for 1 h and incubated with primary antibodies against β-actin (1:1000, Abcam, cat# ab8226), PI3K (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... samples were incubated with a secondary antibody diluted in 1%BSA PBS-0.05%Tween 20 (goat anti-mouse IgG H&L – Alexa fluor 488 preabsorbed, Abcam, cat# ab150081; 1:500) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... After washing the membranes in TBST (3 × 5 min), they were probed (1.5 h, RT) with the HRP-conjugated secondary antibodies: goat anti-mouse IgG (Abcam Cat# ab205719, 1:10000) or goat anti-rabbit IgG (Abcam Cat# ab205718 ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed with TBS-T and then incubated with HRP-conjugated secondary antibodies for 1 h at RT: goat anti-rat IgG at 1:2000 (Abcam, Waltham, MA #ab97057), sheep anti-mouse IgG at 1:2000 (GE Cytiva #NA931) ...
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bioRxiv - Bioengineering 2024Quote: ... Then the membrane was washed three times in TBS-T and incubated at room temperature for 2 hours with the following secondary antibodies goat anti-rabbit IgG H&L (HRP) (Abcam #ab205718, 1:2000) and m-IgG Fc BP-HRP (Santa Cruz ...
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bioRxiv - Physiology 2024Quote: ... and 0.3% Triton X-100 in PBS for 2 h at room temperature and incubated with primary c-FOS antibody (rabbit, Abcam ab190289, 1:1000) overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... brain slices were incubated for 3 h at room temperature with a mix of secondary antibodies (ThermoFisher A-11039 or Abcam ab150158, 1:400) that had been previously centrifuged at 20000g for 10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... The membranes were then blocked using 5% nonfat milk for 1 h and incubated with primary antibodies against VASN (cat# ab156868, abcam, 1:500 dilution), IL33 (cat# A8096 ...
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bioRxiv - Immunology 2021Quote: ... the monolayer was incubated with Donkey Anti-Rabbit IgG H&L-Alexa Fluor® 594 (1:250) (cat. n° ab150072, Abcam, Cambridge, MA, USA) and Goat Anti-Chicken IgY H&L-Alexa Fluor® 488 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... This was followed by a 48h incubation in goat antirabbit IgG H&L (Alexa Fluor 555) antibody (1:500, Abcam, Abcam Cat# ab150078, RRID:AB_2722519) and then placed for 1 h in a Hoescht:PBS (1:10 000 ...
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bioRxiv - Bioengineering 2020Quote: ... cells were incubated at room temperature for 1 hour with the secondary antibody Goat Anti-Mouse IgG H&L-Alexa Fluor® 488 (Abcam, Cambridge, MA, ab150113) and Hoechst 33342 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Cells lysates were either mixed with 3 × Laemmli buffer for immunoblotting analysis or subject to pre-clearing and NDC80 complexes were isolated from 1 mg of cell lysate by 2-h incubation at 4°C with anti-NDC80 antibody cocktail (ProteinTech, 18932-1-AP; Abcam, ab3613; SantaCruz, sc-515550) or ...
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bioRxiv - Molecular Biology 2023Quote: ... The membrane was blocked in 5% milk/PBS for 1 h followed by overnight incubation with mouse monoclonal anti-TFAM antibody (Abcam, #ab119684, 1:1000 dilution) at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS at RT for 1□h and incubated with primary antibodies (PGR - Cell signaling Technology 8757S; ESR1 - Cell signaling Technology 8644S; ZO1 - Invitrogen 33-9100, GFP - Abcam ab5450 ...
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bioRxiv - Bioengineering 2024Quote: ... The following secondary antibodies were added for 1 hour at room temperature: Goat anti-Rabbit IgG (H+L) Alexa Fluor 568 (Abcam, #ab175471, dilution 1:1000) and Goat anti-Mouse IgG (H+L ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then incubated for 16 h O/N at 4°C with the following primary antibodies: CASR (Abcam Cat# ab79038, RRID:AB_2071489, 1:500), MAP2 (Abcam Cat# ab92434 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed with 1xPBST five times followed by incubation with secondary antibody (Goat Anti-Rabbit IgG H&L, Alexa Fluor® 488, Abcam Cat# ab150077, RRID:AB_2630356) for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... Tissue sections on unlabeled slides were stained with hematoxylin and eosin (H&E) and subjected to immunohistochemical (IHC) staining using TRPV4 antibodies (Abcam 39260; 1:100 dilution). Whole-slide images were captured using the Olympus VS200 whole-slide imaging system ...
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bioRxiv - Microbiology 2021Quote: ... incubated at 37 °C for 1 h with a 1:333 dilution of 1 mg/ml anti-VE-cadherin polyclonal antibody (Abcam, Toronto, ON, Canada, cat. ab33168) in PBS−/− with 3% BSA ...
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bioRxiv - Synthetic Biology 2022Quote: ... then incubated for 1 h at 37 °C in the presence of a horseradish peroxidase– conjugated goat anti-mouse IgG antibody (Abcam, Cat # ab97265; diluted 1:25,000). After three PBS-T + 0.3% BSA washes ...
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bioRxiv - Neuroscience 2023Quote: ... 3’,3’diaminobenzidine SDC1-labelled and unlabelled slices counterstained with Haematoxylin were further incubated for 1 h with the rabbit anti-human CHI3L1 antibody (Abcam, Ref ab77528, 1:500 dilution). After three washes ...
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bioRxiv - Neuroscience 2024Quote: ... United States).The following day slices were washed 4 × 10 min followed by 2 h incubation in secondary antibody solution containing goat-anti-rabbit Alexa Fluor 488 (1:500; Abcam, ab150077, Waltham, MA, United States), goat-anti-chicken Alexa Fluor 488 (1:500 ...