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Citations for abcam :
601 - 650 of 854 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was blocked with 5% w/v nonfat dry milk for 1 h at room temperature subsequently probed with mouse monoclonal antibody specific for H3K4me3 and H3K9me3 (1: 2000; abcam) and rabbit polyclonal antibody specific for GAPDH (1:1000 ...
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bioRxiv - Bioengineering 2020Quote: ... Blots were blocked with 3% BSA in TBST (Tris Buffered Saline with Tween 20) for 1 h and then incubated with mouse anti-tau (Abcam) and rabbit anti-beta actin (Abcam ...
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bioRxiv - Immunology 2020Quote: ... 1 mm pieces of small intestine were fixed for 1 h in BD Cytofix/Cytoperm buffer before staining with a rat anti-F4/80 monoclonal (Abcam). Secondary staining was performed using an anti-rat AF647 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... except background IgG was blocked prior to addition of the secondary antibody with Goat F(ab) antimouse IgG (H&L) (Abcam).
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bioRxiv - Synthetic Biology 2020Quote: ... Membranes were blocked with 3% bovine serum albumin in PBS with 0.02% Tween 20 (PBST) for 1 h and then probed with rabbit anti-survivin antibody (1:10000 dilution; Ab 76424, Abcam) at 4°C overnight (16 h) ...
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bioRxiv - Biochemistry 2020Quote: ... Livers and blood samples were collected at this time point for H&E staining and measurement of AST/ALT (Abcam), GSH/GSSG (DojinDo) ...
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bioRxiv - Immunology 2021Quote: ... and Goat Anti-Chicken IgY H&L-Alexa Fluor® 488 (1:1000) (cat. n° ab150169, Abcam, Cambridge, MA, USA) for 60 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... were incubated at 4°C for at least 18 h with either anti-RBM3 monoclonal antibody (Abcam, Cambridge, United Kingdom) or anti-rabbit-IgG-antibody (Cell Signaling ...
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bioRxiv - Cancer Biology 2021Quote: ... They were detected with corresponding secondary antibody: i) donkey anti-sheep IgG H&L (HRP polymer) (catalog no. ab214884, Abcam) or ii ...
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bioRxiv - Cancer Biology 2020Quote: ... The membranes were subsequently subjected to 2 h of incubation with the appropriate corresponding horseradish peroxidase-conjugated secondary antibody (Abcam). Immunoblots were visualized by enhanced chemiluminescence (ECL kit ...
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bioRxiv - Microbiology 2021Quote: ... The plate was washed three times with 1xPBS and then incubated with a fluorescence goat anti-mouse IgG heavy plus light chain (H+L)–Alexa Fluor 488 antibody (Abcam) or an anti-human secondary (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed and bound specific IgG was detected with a HRP-conjugated goat anti-mouse IgG H&L antibody (ab6789, Abcam) diluted 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... at room temperature for 2 h and then incubated overnight at 4°C in Blocking Buffer containing primary antibody (anti-mouse PIWIL2/MILI, Abcam Cat# ab36764 ...
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bioRxiv - Immunology 2021Quote: ... Antibody staining was typically conducted on ice for 1 h with rat monoclonal FITC or PE anti-mouse Ly6B.2 (clone 7/4; Abcam), FITC or PE/Cy5 anti-mouse F4/80 (clone BM8 ...
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bioRxiv - Genomics 2021Quote: ... The fluorophore-conjugated secondary antibodies include Goat anti-Rabbit IgG H&L-Alexa Fluor 488 (1:500, Abcam, Cat#ab150077) and Goat anti-Rabbit IgG H&L-Alexa Fluor 555 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were incubated for at least 72 h with the primary antibodies anti-GFP (chicken, 1:2000; Abcam, Cambridge, UK), anti-nc82 (mouse ...
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bioRxiv - Neuroscience 2022Quote: ... + protease inhibitors added fresh) for 1 h prior to SDS-PAGE and western blotting with SEZ6L2 (Abcam, ab197058, 1:500), PRRT2 (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... HUVECs were washed three times with 1X PBS and then blocked at room temperature for 1 h with a 2.5% goat serum (Abcam, ab7481) solution ...
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bioRxiv - Biochemistry 2022Quote: ... proteins was confirmed with western blot (anti-G3BP1, 1:1,000 dilution, Santa Cruz, H-10; anti-GFP, 1:2,000 dilution, Abcam, ab290).
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bioRxiv - Genetics 2022Quote: ... The primary antibodies were diluted in 10% donkey serum with PBST in various dilution as described in (H. L. Ou & Schumacher, 2021) (rabbit anti-V5 1:50 [Abcam, ab206566] ...
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bioRxiv - Plant Biology 2022Quote: ... Sections were incubated with blocking buffer [3% (m/v) BSA in PBS buffer] for 1 h before overnight incubation with primary antibodies for H3K9me2 (1:400 dilution, abcam) and H3K27me1 (1:600 dilution ...
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bioRxiv - Microbiology 2023Quote: ... The apical side of Caco-2 cells grown on Transwell inserts were exposed for 3 h to 20 nM Axl inhibitor (R428) (Abcam), 1 μg of anti-Gas6 antibody (R&D Systems) ...
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bioRxiv - Microbiology 2022Quote: ... Anti-ATG8 was used in conjunction goat Anti-guinea pig IgG H&L (Alexa Fluor® 488) (Abcam, Waltham, MA). Bound antibodies were detected on a Bio-Rad Imager and quantified using Image Lab Software (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... 5% CO2 for 24 h before supernatants were collected and LDH levels determined using an LDH-Cytotoxicity Assay Kit (abcam).
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bioRxiv - Neuroscience 2024Quote: ... the cells were blocked in 5% BSA diluted in PBS-Triton X-100 (0.25%) for 1 h at room temperature and stained for GFP (Abcam ab13970) for 1.5 h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was denatured with 2.5 M HCl for 1 h and incubated with 1:1000 rat anti-BrdU (Abcam, ab6326) for 1 h at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... at 4°C for 48 h and then for 2 h at room temperature with the fluorescent secondary antibodies Alexa-555 donkey anti-rabbit (1:1000; Abcam), Alexa-633 donkey anti-mouse (1:250 ...
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bioRxiv - Molecular Biology 2024Quote: AP-sites were tested in U2OS cells 48 h after transfection with control and UNG siRNA by a DNA damage assay kit (ab211154, Abcam) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... The membrane was then washed with Tris-Buffered Saline with Tween-20 and incubated in one of the following secondary antibodies: Goat Anti-Rabbit IgG H&L (HRP) (Abcam, ab6721 ...
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bioRxiv - Neuroscience 2024Quote: ... co-staining IBA-1 – ZO-1 incubation during 24 h at room temperature (1:1000, ab178846, rabbit anti-IBA-1, Abcam; 1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... incubated with the following primary antibody diluted in PBS-Tween (0.1%) for 1 h at RT: anti-Olig2 (1:100, Abcam, #ab109186), anti-Cleaved caspase 3 (1:100 ...
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bioRxiv - Cell Biology 2024Quote: ... samples were incubated for 2 h at room temperature with anti-rabbit IgG Alexa Fluor 488 (150081; Abcam, Boston, USA) and anti-mouse IgG Alexa Fluor 546 (Invitrogen A1003 ...
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bioRxiv - Bioengineering 2024Quote: ... where the middle sections were stained using H&E and the two sections on the sides were respectively double-stained immunohistochemically with CCR7 (Y59, abcam,Cat#ab32527 ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were blocked with BloxAll blocking buffer and 2.5% horse serum respectively prior to a 1-h incubation with anti-Ki67 antibody (Abcam, ab16667) at 1:100 dilution ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were washed three times with PBS and incubated for 1 h at room temperature with Alexa Fluor 555-conjugated donkey anti-rabbit antibody (ab150074, Abcam) or Alexa Fluor 488-conjugated donkey anti-mouse antibody (ab150105 ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were washed with PTX for 3 × 10 min at RT and incubated with secondary antibodies in PTX (1:200 goat anti-chicken IgY H&L DyLight 488 (Abcam)) for 2 h at RT ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h at room temperature and probed with the primary antibodies to Slack (1:3000, AvesLabs) and HCN1 (1:1000, Abcam) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... and further incubated for 1 h at room temperature with FITC-labeled anti-PECAM-1 antibody (Platelet Endothelial Cell Adhesion Molecule-1, Abcam) diluted 1:1000 in blocking buffer before being washed twice with PBS-T ...
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bioRxiv - Microbiology 2023Quote: ... Stained cells were than washed with PBS twice and incubated with 1:500 AF488 fluorochrome-conjugated goat anti-mouse IgG H&L (Abcam) in the staining buffer for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with the following primary antibodies (12 h at 4°C): anti-Vglut1 (1:200, rabbit, Abcam, ab227805), anti-Vgat (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were washed and incubated for 1 H in a 1:200 primary solution of Anti-pRAB7A antibody (ab302494, Abcam).
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were then incubated for 8–10 h with the following primary antibodies: anti-CLPP (ab124822, Abcam, Cambridge, UK), anti-PTEN-induced kinase 1 (PINK1 ...
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bioRxiv - Developmental Biology 2023Quote: ... the samples were washed with several changes of PBS over minimum of 4 h at room temperature and mounted on microscopy slides with Anti-Fade Fluorescense Mounting Medium (Abcam).
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bioRxiv - Molecular Biology 2023Quote: ... slides were incubated with secondary antibody Goat Anti Rabbit IgG H&L (Alexa Fluor 488) (1:500 dilution, Abcam, ab150077) for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The CldU and IdU tracks were stained for 2.5 h with anti-BrdU primary antibodies recognizing CldU (1:500; Abcam; ab6326) and IdU (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was then blocked with 5% skim milk at 37 °C for 1 h and incubated overnight with the following antibodies: anti-Alix (1:1,000, ab275377, Abcam, UK), anti-TSG101 (1:1,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Horseradish peroxidase-conjugated goat anti-Mouse (ab6789) and donkey anti-Mouse IgG H&L (Alexa Fluor 555) (ab150110) were from Abcam. All antibodies were diluted 1:1000 for Western blot and 1:100 for immunofluorescence.
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bioRxiv - Biophysics 2023Quote: ... YAP was stained mouse polyclonal anti-human YAP (sc-101199, 1:200, Santacruz) and Alexa Fluor 488 goat anti-mouse IgG H&L (ab150077, 1:200; Abcam). Phalloidin labeled with Alexa Fluor 488-conjugated phalloidin (YP0052L ...
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bioRxiv - Neuroscience 2023Quote: ... then they were incubated (12-24 h at 4 °C) with the primary antibodies anti-TPH2 (1:500, rabbit, Abcam), anti-Vglut3 (1:500 ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated at RT for 1hr in secondary antibody diluted in TBST-5% milk (1:4000 ab6721 Goat Anti-Rabbit IgG H&L HRP (Abcam), 1:4000 ab205719 Goat Anti-Mouse IgG H&L HRP (Abcam)) ...