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Citations for abcam :
751 - 800 of 854 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Cell culture supernatant was collected at 24 h after transfection and subjected to ELISA assays to measure the hEPO expression using Human Erythropoietin ELISA Kit (Abcam, ab274397).
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bioRxiv - Immunology 2024Quote: ... Detection of the rabbit pAb cocktail was conducted with a goat anti-rabbit IgG (H+L) AF647 secondary antibody (Abcam ab150079). Flow cytometry was used for quantifying binding using an Agilent NovoCyte Advanteon flow cytometer.
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bioRxiv - Bioengineering 2024Quote: ... the slides were incubated with secondary antibody Goat Anti Rabbit IgG H&L (Alexa Fluor 488) (Abcam, ab150077, 1:500 dilution) for 30 min at 37℃ ...
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bioRxiv - Bioengineering 2024Quote: ... at 37°C for 20 min followed by blocking in PBS containing 5% bovine serum albumin and 0.1% Tween 20 for 1 h before incubating with rabbit anti-collagen type II (Abcam, 1:100) primary antibody at 4°C overnight ...
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bioRxiv - Biophysics 2024Quote: ... at a ratio of 1:5000 in Roti®-Block in TBS-T and the goat-anti-rabbit IgG H&L (HRP) preadsorbed (#ab7090, abcam) at a ratio of 1:20,000 in TBS-T as a secondary antibody.
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bioRxiv - Bioengineering 2024Quote: ... The plates were incubated with 100 µl of goat anti-human IgG (H+L) cross-adsorbed secondary Ab (Abcam, Cambridge, UK) at 1/10,000 dilution in TBS-T for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were blocked in 10% goat serum in 1X PBS-T for 1 h and then incubated with primary antibody rabbit anti-mouse CDX2 (Abcam, ab76541) at a 1/1000 dilution in 1X PBS-T overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were washed four times with staining buffer before applying the secondary antibody (1:800 donkey anti-rat IgG H&L Alexa Fluor-647, Abcam, ab150155) for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... at room temperature in the dark for 1 h at 1:1000 dilution (anti-rabbit Alexa488, Abcam ab150077; anti-mouse Cy3, Abcam ab97035). Cells were then washed with 2x with PBS for 5 min per wash before ProLongTM Gold antifade reagent with DAPI (#P36931 ...
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bioRxiv - Cell Biology 2024Quote: ... The 2 or 4 µm section was used for H&E staining and IHC of anti-human CD11b mAb (abcam #ab307523). The samples were observed by Nikon Eclipse Ti2 microscope carrying NIS-Elements imaging software.
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bioRxiv - Cancer Biology 2021Quote: ... were incubated for 1 h at room temperature, CD4 (ZM0418, clone UMAB64, Zsbio, 1:200) and CD133 (ab19898, polyclonal antibody, abcam, 1:400) were incubated for overnight at 4□.
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bioRxiv - Immunology 2021Quote: ... confluent monolayers of 293T Lenti-X cells were stained with 2G12 or PGT145 bNAbs and Goat Anti-Human IgG H&L (10 nm Gold) secondary Ab (Abcam, Cambridge, UK) and then fixed in 2.5% glutaraldehyde in cacodylate buffer 0.1 M ...
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bioRxiv - Developmental Biology 2020Quote: ... brains were incubated with conjugated fluorescent secondary Alexa Fluor 555 donkey anti-mouse IgG (H+L) (Abcam; Material No. ab150106; 1:250) for 2 days at 4 °C.
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bioRxiv - Bioengineering 2022Quote: ... Cells were again washed in DPBS and incubated for 1 h at room temperature with Alexa Fluor® 488-conjugated secondary antibody (goat polyclonal antibody to rabbit IgG, 1:500, Abcam, Ab15077). Nuclei were counterstained with 4’,6’-diamidino-2-phenyliondole (DAPI ...
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bioRxiv - Genomics 2020Quote: ... Blocking with 1% BSA/PBS for 1h was followed by incubation with the following primary antibodies for 1-2 h at the indicated dilution: mouse monoclonal anti-HMGB1 (1:1000; Abcam ab190377-1F3); rabbit polyclonal anti-HMGB2 (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... Spore suspensions were then incubated for 1 h with 1:20 donkey anti-rabbit IgG antibody coupled to 12–nm gold particles (Abcam ab105295, USA) in PBS-1% BSA for 1h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were then washed with PBS and incubated for 24 h at 4 °C with anti-BDNF (Abcam, USA, 1:400), anti-doublecortin (DCX ...
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bioRxiv - Neuroscience 2020Quote: ... the samples were incubated for 3 h at room temperature with the goat anti-chicken antibody conjugated with Alexa Fluor® 488 (Abcam 150169) at a concentration of 1:500 in PBS-blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Then the tissue sections were incubated overnight at 4 degrees Celsius with the primary goat polyclonal to cfos (1:500, 24 h, abcam; RRID: SCR_012931) primary antibody ...
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bioRxiv - Bioengineering 2020Quote: ... secondary antibody was subsequently applied for 1 h at room temperature and the samples were mounted in Fluoroshield Mounting Medium with DAPI (Abcam, Cambridge, USA). Stained sections were visualized with the EVOS® FL Cell Imaging System ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... sections were incubated for 72 h at 4 °C with gentle agitation with rabbit polyclonal anti GLS2 (ab113509; 1:1000, Abcam, Cambridge, UK) and sheep polyclonal anti pS6 (ab65748 ...
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bioRxiv - Bioengineering 2022Quote: ... and incubated for 1h at room temperature with anti-mouse or anti-rabbit IgG H&L secondary antibodies conjugated with Alexa Fluor™ 488 or Alexa Fluor™ 568 (goat, 1:500; Abcam). Slides were mounted with VECTASHIELD antifade mounting medium containing DAPI (Vector Labs ...
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bioRxiv - Molecular Biology 2022Quote: ... NAD(P)H quinone dehydrogenase 1 (NQO-1; #ab80588) and pan-glyceraldehyde-3-phosphate dehydrogenase (GAPDH; #ab8245) antibodies were provided by Abcam (Cambridge, England). The p38 MAPK inhibitor SB203580 (#S8307 ...
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bioRxiv - Biophysics 2022Quote: ... The cells were washed with PBS three times before labeling with goat anti-rabbit IgG H&L Biotin (ab97049; abcam, Cambridge, UK) at 1:500 dilution for 30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... sections were washed 3x with PBS with 0.1% Tween-20 before incubation with the secondary polyclonal Donkey Anti-Goat IgG H&L antibody conjugated to Alexa Fluor 555 (ab150130; Abcam; 1:200) or Donkey Anti-Rabbit IgG H&L antibody conjugated to Alexa Fluor 555 (ab150066 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were washed 3x with 100 μL PBS with 0.1% Tween-20 before incubation with the secondary polyclonal Donkey Anti-Goat IgG H&L antibody conjugated to Alexa Fluor 555 (ab150130; Abcam; 1:200) or Donkey Anti-Rabbit IgG H&L antibody conjugated to Alexa Fluor 555 (ab150066 ...
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bioRxiv - Immunology 2020Quote: ... and equal amounts of fragmented chromatin–protein complexes were incubated for 2 h with enclosed Dynabeads coupled to rabbit anti-Polβ (Ab26343, Abcam, Cambridge, UK), mouse anti-MSH2 mAb (FE11 ...
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bioRxiv - Neuroscience 2020Quote: ... The retinas were incubated in PBS with 1% Triton X-100 and 0.5% Tween 20 for 1 h at room temperature and in 4% BSA for 1 h at room temperature and then incubated overnight at 4 °C with primary antibodies: RBPMS (1:500, Abcam, Cambridge, UK), ChAT (1:100 ...
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bioRxiv - Bioengineering 2021Quote: ... and 1% penicillin/streptomycin in PBS (Ca2+,Mg2+)) containing 30 µg/mL Goat anti-human IgG H&L (Dylight® 488, Abcam ab96911) at a 1:1 ratio ...
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bioRxiv - Cell Biology 2022Quote: ... and 1% horse serum for 1.5 h at room temperature and incubated with primary antibodies [anti-Pax7 (rabbit-polyclonal, Abcam, 1:500 dilution) and anti-MyoD (mouse-monoclonal ...
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bioRxiv - Synthetic Biology 2020Quote: ... The concentrations of NAD(H) (total NAD+/NADH) and NADH only were measured by the NAD+/NADH assay kit (Abcam, Cambridge, UK) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... the channels were incubated at 4 °C for 16 h with the following primary antibodies: anti-mouse laminin MEC5 (rat IgG; Catalog number ab2466; 1:50; Abcam, Cambridge, UK), anti-mouse collagen IV (rabbit IgG ...
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bioRxiv - Immunology 2021Quote: ... iPS cells were treated for 1 h with HDR enhancer (5 μM; IDT, Coralville, USA) and 10 μM Y-27632 (Abcam, Cambridge, UK). Electroporated cells were seeded on Laminin 521 (Biolamina ...
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bioRxiv - Physiology 2020Quote: ... was incubated for 1 hour at RT followed by 1 h incubation with streptavidin-DyLight 647 (Vector, 1:300) and anti-rat DyLight 594 (Abcam, 1:300) and subsequently 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the sections were incubated overnight (16 h) in a humid chamber at 4°C with either rabbit SHH antibody (2 μg/ml; ab19897; Abcam, Cambridge, UK), mouse Col2 antibody (1/50 dilution ...
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bioRxiv - Microbiology 2020Quote: ... 293T cells were fixed and processed for immunostaining against dsRNA (Scicons, 10010500) and visualized with goat anti-mouse IgG H&L (Cy5 ®) (Abcam, ab6563). Images were taken using Nikon Eclipse Ti-E Spinning Disk under 20X and 60X ...
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bioRxiv - Cell Biology 2021Quote: ... lungs were incubated with conjugated fluorescent secondary Alexa Fluor 555 donkey anti-mouse IgG (H+L) (Abcam; Material No. ab150106; 1:250) for two days at 4 °C.
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bioRxiv - Immunology 2022Quote: ... Slides containing 4 sections per slide and 8 sections per sample were incubated with primary antibodies at 4°C overnight and then washed three times with PBS before incubating them with goat anti-mouse IgG (H&L) secondary antibody (Alexa Fluor® 488 in 1% BSA PBST, 1:300; Abcam #ab150113) for 1 h at room temperature in the dark ...
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bioRxiv - Synthetic Biology 2022Quote: ... 0.1% Tween 20) and subsequently probed for 1 h with one of the following: horseradish peroxidase (HRP)-conjugated anti-DDDDK antibody (Abcam, cat # ab49763) that recognized the FLAG epitope tag ...
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bioRxiv - Neuroscience 2022Quote: ... The sections containing hippocampus were blocked with 10% donkey serum with 0.3% Triton X-100 for 2 h at RT and incubation overnight at 4℃ with corresponding primary antibodies: goat anti-IBA-I (1:1000, Abcam, Cambridge, UK), chicken anti-YFP/GFP (1:500 ...
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bioRxiv - Bioengineering 2022Quote: ... anti-occludin antibody (ab216327, and goat anti-rabbit IgG H&L (Alexa Fluor 488, ab150077) antibody were all obtained from Abcam (Waltham, MA). Anti-tumor necrosis factor-α (aTNF ...
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bioRxiv - Physiology 2024Quote: ... Membranes were blocked with 5% BSA in TBS-T for 1h before overnight incubation with rabbit anti-FXR (H-130; 1:2.000) and mouse anti-β-actin (ab6276, Abcam; 1:10.000) followed by incubation with goat anti-rabbit-IgG-AlexaFluor 647 and goat anti-mouse-IgG-AlexaFluor 488 (both at 1:5.000) ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibody: Goat Anti-Rabbit IgG H&L(Alexa Fluor® 647)preadsorbed (ab150083) at a dilution of 1:2000 (Abcam, USA).
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibody: Goat Anti-Rabbit IgG H&L(Alexa Fluor® 647)preadsorbed (ab150083) at a dilution of 1:2000 (Abcam, USA); Ro 3306 ...
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bioRxiv - Neuroscience 2024Quote: ... Triton X-100 in PBS and incubated with donkey-anti-mouse IgG (H+L) Alexa Fluor 488 nm (1:500, Abcam, UK, ab150109), donkey-anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: ... The tissue was incubated for 22-24 h at 4 °C in a solution of primary antibody (1:2000 rabbit polyclonal to c-Fos, Abcam, Cambridge, MA) with 5% normal goat serum (Abcam ...
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bioRxiv - Immunology 2024Quote: ... samples were treated with unconjugated affinity purified F(ab) fragment IgG anti-mouse (H+L) for 1 hr at RT (0.1 mg/ml, Abcam, cat no. ab6668). Mouse epithelium was stained with Alexa 633-conjugated phalloidin (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Stripped membranes were blocked in 5% NFDM in TBST (1 h, RT) before being probed with anti-Act1 (Abcam ab8224, 1:5000) loading control antibody (1 h ...
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bioRxiv - Genomics 2023Quote: ... cells were blocked for 1 h in 1×PBS + 2% w/v BSA and then incubated with mouse anti-E-cadherin (1:1000) (Abcam, cat#231303) and chicken anti-vimentin (1:200 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were stained for 30 min with polyclonal fluorescein-isothiocyanate (FITC)-conjugated goat anti-mouse IgG H&L specific antibody (Abcam, United Kingdom) diluted 1:50 in RS-BSA 0.1% ...