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• SARS-COV-2 nucleocapsid protein hijacks multiple components of the host nuclear transport machinery for distinct functions

  Angela R. Harrison, et al., bioRxiv - Microbiology 2024

  Quote: ... GFP (Roche, 11814460001 and Abcam, ab290), β-actin (Cell Signaling Technology ...
• SARS-COV-2 nucleocapsid protein hijacks multiple components of the host nuclear transport machinery for distinct functions

  Angela R. Harrison, et al., bioRxiv - Microbiology 2024

  Quote: ... anti-KPNA3 (Abcam, ab6038) and anti-KPNA4 (Abcam ...
• SARS-COV-2 nucleocapsid protein hijacks multiple components of the host nuclear transport machinery for distinct functions

  Angela R. Harrison, et al., bioRxiv - Microbiology 2024

  Quote: ... anti-KPNA1 (Abcam, ab154399), anti-KPNA2 (BD Biosciences ...
• SARS-COV-2 nucleocapsid protein hijacks multiple components of the host nuclear transport machinery for distinct functions

  Angela R. Harrison, et al., bioRxiv - Microbiology 2024

  Quote: ... and anti-KPNA4 (Abcam, ab6039). Coverslips were then washed five times with PBS before staining with Alexa Fluor 568 secondary antibodies (Thermo Fisher Scientific ...
• A complete approach for circRNA therapeutics from purification to lyophilized delivery using novel ionizable lipids

  Esther Broset, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Membranes were washed with TBS-T buffer and incubated with HRP-conjugated goat anti-mouse IgG (Abcam). Following additional wash with TBS-T ...
• HuR-dependent expression of RyR2 contributes to calcium-mediated thermogenesis in brown adipocytes

  Adrienne R. Guarnieri, et al., bioRxiv - Molecular Biology 2024

  Quote: ... in conjunction with 4 ug of HuR antibody (AbCam ab200342) as previously described by our lab.21 RNA was eluted and lysate samples were spiked with 5 ng of Luciferase control RNA (Promega L4561 ...
• Functional interaction between transcription factor Sfp1 and the NuA4 complex in response to nutrient availability

  Ke Xu, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Antibodies used were against H4K8ac (Abcam, ab45166), H3K9ac (Upstate ...
• Integrated hepatic ferroptosis gene signature dictates pathogenic features of ferroptosis

  Takashi Matsumoto, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Tissues were then stained with antibodies against 4-hydroxynonenal (4-HNE; Abcam, Cat# ab46545) according to standard procedures and counterstained with hematoxylin solution for 30 s ...
• A conjugative plasmid exploits flagella rotation as a cue to facilitate its transfer

  Saurabh Bhattacharya, et al., bioRxiv - Microbiology 2024

  Quote: ... and subsequently treated with Alexa 647 conjugated secondary antibody (1:2000, Abcam) for 30 minutes at room temperature ...
• Cell division protein A (CdpA) assembles and anchors the two FtsZ rings at midcell in haloarchaea

  Yan Liao, et al., bioRxiv - Microbiology 2024

  Quote: ... Following that the membrane was incubated with the secondary antibody against mouse (Goat anti-mouse IgG-HRP; AbCam 6789) for 1 h in a 1:5,000 dilution ...
• Extracts of food and medicinal plants sold in Moroccan markets induce apoptosis-like in Toxoplasma gondii tachyzoites in vitro

  Ismail Elkoraichi, et al., bioRxiv - Microbiology 2024

  Quote: ... tachyzoites were stained with 2 µL of Annexin-V-FITC and 2 µL of propidium iodide (ab 14085, Abcam) in 100 µL annexin-V buffer (ab… Abcam ...
• Extracts of food and medicinal plants sold in Moroccan markets induce apoptosis-like in Toxoplasma gondii tachyzoites in vitro

  Ismail Elkoraichi, et al., bioRxiv - Microbiology 2024

  Quote: ... in 100 µL annexin-V buffer (ab… Abcam) for 15 min at 4 °C in the dark ...
• Multiple cell types support productive infection and dynamic translocation of infectious Ebola virus to the surface of human skin

  Kelly N. Messingham, et al., bioRxiv - Microbiology 2024

  Quote: ... 72 h post-transfection cells in 6 well format were harvested in 1% SDS to assess knockdown efficiency via immunoblot (Abcam, #ab134113).
• Exploiting the Achilles’ Heel of Viral RNA Processing to Develop Novel Antivirals

  Ali Zahedi Amiri, et al., bioRxiv - Microbiology 2024

  Quote: ... rabbit α-CLK2 (Abcam, Cat: ab65082), mouse α-CLK3 (Abnova ...
• Exploiting the Achilles’ Heel of Viral RNA Processing to Develop Novel Antivirals

  Ali Zahedi Amiri, et al., bioRxiv - Microbiology 2024

  Quote: ... rabbit α-Tra2β (Abcam, Cat: ab31353), mouse α-CLK1 (Santa Cruz ...
• Protection against Neisseria meningitidis nasopharyngeal colonization relies on antibody opsonization and phagocytosis by neutrophils

  Elissa G. Currie, et al., bioRxiv - Microbiology 2024

  Quote: ... AP-goat-anti-mouse IgA (Abcam ab97232), AP-goat-anti-mouse IgG1 (Jackson 115-055-205) ...
• P-BODY AND CYTOSKELETON REMODELING BY ORTHOHANTAVIRUSES

  Hannah Sabeth Schwarzer-Sperber, et al., bioRxiv - Microbiology 2024

  Quote: ... anti-Dcp1a for P-body detection (1:100, #ab57654, Abcam, Cambridge, UK), anti-α-tubulin (1:1000 ...
• Typhoid toxin of Salmonella Typhi elicits host antimicrobial response during acute typhoid fever

  Salma Srour, et al., bioRxiv - Microbiology 2024

  Quote: ... and LYZ ELISA kits (Abcam, ab108880) were used as per manufacturer’s instructions ...
• Trehalose dimycolate inhibits phagosome maturation and promotes intracellular M. tuberculosis growth via noncanonical SNARE interaction

  Carolina Santamaria, et al., bioRxiv - Microbiology 2024

  Quote: ... To test for O-x-AlkTDM presence we used in-gel fluorescence (to detect TAMRA) and/or immunoblotted with anti-biotin antibodies (1:1000; 5% nonfat Milk in TBST; Abcam).
• Chemical degradation identified Human p38-MAPK as a host target to combat parasitic infections

  Jhalak Singhal, et al., bioRxiv - Microbiology 2024

  Quote: ... following the guidelines provided by the manufacturer (Abcam #ab113851). Once the incubation period concluded ...
• The last resort antibiotic daptomycin exhibits two independent antibacterial mechanisms of action

  Jessica A. Buttress, et al., bioRxiv - Microbiology 2024

  Quote: Daptomycin was purchased from Abcam; nisin ...
• PfATP2 is an essential flippase on the Plasmodium falciparum surface that influences parasite sensitivity to antiplasmodial compounds

  Deyun Qiu, Eleanor England, Adele M. Lehane, bioRxiv - Microbiology 2024

  Quote: ... The secondary antibodies used were an anti-rat goat horseradish peroxidase (HRP)-conjugated antibody (1:5000 dilution, ab97057, Abcam) or an anti-mouse goat HRP-conjugated antibody (1:5000 dilution ...
• PfATP2 is an essential flippase on the Plasmodium falciparum surface that influences parasite sensitivity to antiplasmodial compounds

  Deyun Qiu, Eleanor England, Adele M. Lehane, bioRxiv - Microbiology 2024

  Quote: ... or an anti-mouse goat HRP-conjugated antibody (1:5000 dilution, ab6789, Abcam).
• Hemin-binding DNA structures on the surface of bacteria promote extracellular electron transfer

  Obinna M. Ajunwa, et al., bioRxiv - Microbiology 2024

  Quote: ... B-DNA specific antibody AB1.227156 (mouse monoclonal, AB27156, 1068371-2) was purchased from Abcam® without a fluorescent label ...
• Periplasmic serine protease Prc is responsible for amyloid subunit CsgA degradation and proteostasis in Escherichia coli

  Shinya Sugimoto, et al., bioRxiv - Microbiology 2024

  Quote: ... anti-MBP (Abcam, Cambridge, MA, USA), anti-OmpA (gifted by Dr ...
• Periplasmic serine protease Prc is responsible for amyloid subunit CsgA degradation and proteostasis in Escherichia coli

  Shinya Sugimoto, et al., bioRxiv - Microbiology 2024

  Quote: ... and anti-RpoD (Abcam) antibodies were used as previously reported (13 ...
• Pathogenic mechanisms of Plasmodium falciparum egress unveiled by a microvascular 3D blood-brain barrier model

  Livia Piatti, et al., bioRxiv - Microbiology 2024

  Quote: ... GFAP (Abcam ab4674), S100B (Sigma S2532-100U) ...
• Pathogenic mechanisms of Plasmodium falciparum egress unveiled by a microvascular 3D blood-brain barrier model

  Livia Piatti, et al., bioRxiv - Microbiology 2024

  Quote: ... ICAM-1 (Abcam ab20), GFAP (Abcam ab4674) ...
• Pathogenic mechanisms of Plasmodium falciparum egress unveiled by a microvascular 3D blood-brain barrier model

  Livia Piatti, et al., bioRxiv - Microbiology 2024

  Quote: ... αSMA (Abcam ab202509). After six 10-minute PBS washes ...
• Pathogenic mechanisms of Plasmodium falciparum egress unveiled by a microvascular 3D blood-brain barrier model

  Livia Piatti, et al., bioRxiv - Microbiology 2024

  Quote: ... PDGFRβ (Abcam ab69506), NG2 (Invitrogen 372700) ...
• Pathogenic mechanisms of Plasmodium falciparum egress unveiled by a microvascular 3D blood-brain barrier model

  Livia Piatti, et al., bioRxiv - Microbiology 2024

  Quote: ... Primary antibodies against the following proteins were used: VE-cadherin (Santa Cruz Biotechnology sc-52751 or Abcam ab33168), STAT1 (Cell Signaling 14994S) ...
• Pathogenic mechanisms of Plasmodium falciparum egress unveiled by a microvascular 3D blood-brain barrier model

  Livia Piatti, et al., bioRxiv - Microbiology 2024

  Quote: ... VE-cadherin (Santa Cruz Biotechnology sc-52751 or Abcam ab33168), STAT1 (Cell Signaling 14994S) ...
• Pathogenic mechanisms of Plasmodium falciparum egress unveiled by a microvascular 3D blood-brain barrier model

  Livia Piatti, et al., bioRxiv - Microbiology 2024

  Quote: ... αSMA (Abcam ab202509), PDGFRβ (Abcam ab69506) ...
• Divergence in poxvirus-encoded E3-like proteins can dictate poxvirus activation of cellular necroptosis

  Junior A. Enow, et al., bioRxiv - Microbiology 2024

  Quote: ... human MLKL (Abcam# ab243142 ...
• Divergence in poxvirus-encoded E3-like proteins can dictate poxvirus activation of cellular necroptosis

  Junior A. Enow, et al., bioRxiv - Microbiology 2024

  Quote: ... mouse pMLKL (abcam # ab196436, ), mouse MLKL (cell signaling # 37705S) ...
• Capture of fusion-intermediate conformations of SARS-CoV-2 spike requires receptor binding and cleavage at either the S1/S2 or S2’ site

  Sabrina Lusvarghi, et al., bioRxiv - Microbiology 2024

  Quote: ... Proteins were stained using InstantBlue Coomassie Protein Stain (Abcam).
• Mitophagosomes induced during EV-D68 infection promote viral nonlytic release

  Alagie Jassey, et al., bioRxiv - Microbiology 2024

  Quote: ... Fluo-8 Calcium Assay Kit – No Wash (Abcam, ab112129) was used following the manufacturer’s instructions.
• Mitophagosomes induced during EV-D68 infection promote viral nonlytic release

  Alagie Jassey, et al., bioRxiv - Microbiology 2024

  Quote: ... Rabbit monoclonal-anti-TOMM20 (Abcam, ab186735). Rabbit polyclonal-anti-Cox4 (Cell Signaling ...
• Exploring virus-host interactions through combined proteomic approaches identifies BANF1 as a new essential factor for African Swine Fever Virus

  Juliette Dupré, et al., bioRxiv - Microbiology 2024

  Quote: ... rabbit anti-BANF1 (ab129184, Abcam). Additionally ...
• Virion Proteomics of Wildtype HCMV Reveals a Novel Regulator of Envelope Glycoprotein Composition that Protects Against Humoral Immunity

  Kirsten Bentley, et al., bioRxiv - Microbiology 2024

  Quote: ... Lysates were incubated with anti-V5 or anti-HA agarose beads (Abcam) overnight ...
• A CRISPRi Library Screen in Group B Streptococcus Identifies Surface Immunogenic Protein (Sip) as a Mediator of Multiple Host Interactions

  K Firestone, et al., bioRxiv - Microbiology 2024

  Quote: ... Rabbit polyclonal anti-GBS antibody was purchased from Abcam (Cambridge, UK).
• A CRISPRi Library Screen in Group B Streptococcus Identifies Surface Immunogenic Protein (Sip) as a Mediator of Multiple Host Interactions

  K Firestone, et al., bioRxiv - Microbiology 2024

  Quote: ... Slides were stained with a 1:100 dilution of the rabbit polyclonal anti-GBS antibody (ab78846; Abcam) for 1 hour ...
• Interferon-β induction heterogeneity during KSHV infection is determined by interferon-β enhanceosome transcription factors other than IRF3

  Machika Kaku, Marta Maria Gaglia, bioRxiv - Microbiology 2024

  Quote: ... antibodies against β-actin (Abcam, ab8229) were used for loading controls ...
• Mammalian ZAP and KHNYN independently restrict CpG-enriched avian viruses

  Jordan T. Becker, et al., bioRxiv - Microbiology 2024

  Quote: ... Primary antibodies used include rabbit anti-ZAP (Abcam, ab154680, 1:5,000 dilution), rabbit anti-KHNYN (Invitrogen ...
• Structural and functional characterization of the KHNYN extended-diKH domain for mediating ZAP antiviral activity

  Rebecca L Youle, et al., bioRxiv - Microbiology 2024

  Quote: ... 1:2000 anti-β-actin (Mouse, Abcam; ab6276), 1:10000 anti-GAPDH (Rabbit ...
• Mammalian ZAP and KHNYN independently restrict CpG-enriched avian viruses

  Jordan T. Becker, et al., bioRxiv - Microbiology 2024

  Quote: ... rabbit anti- TRIM25 (Abcam, ab167154, 1:10,000), and rabbit anti-H3 (Abcam ...
• Mammalian ZAP and KHNYN independently restrict CpG-enriched avian viruses

  Jordan T. Becker, et al., bioRxiv - Microbiology 2024

  Quote: ... and rabbit anti-H3 (Abcam, ab1791, 1:10,000). Secondary antibodies used include goat anti-mouse IgG IRDye 680LT and goat anti-rabbit IgG IRDye 800CW (both used at 1:10,000 dilution in 2% milk in PBST) ...
• Structural and functional characterization of the KHNYN extended-diKH domain for mediating ZAP antiviral activity

  Rebecca L Youle, et al., bioRxiv - Microbiology 2024

  Quote: ... 1:5000 anti-ZAP (Rabbit, Abcam, ab154680), 1:2000 anti-β-actin (Mouse ...
• Expression of Intron-containing HIV-1 RNA Induces NLRP1 Inflammasome Activation in Myeloid Cells

  Sallieu Jalloh, et al., bioRxiv - Microbiology 2024

  Quote: ... mouse anti-SAMHD1 (Abcam, # ab67820, 1:1000), rabbit anti-phospho-SAMHD1 (Cell Signaling ...
• Expression of Intron-containing HIV-1 RNA Induces NLRP1 Inflammasome Activation in Myeloid Cells

  Sallieu Jalloh, et al., bioRxiv - Microbiology 2024

  Quote: ... rabbit anti-CARD8 (Abcam, #ab194585, 1:1000), rabbit anti-MAVS (Invitrogen ...