Labshake search

5301 - 5350 of 10000+ citations since 2020

• Mesoscale regulation of MTOCs by the E3 ligase TRIM37

  Zhong Y. Yeow, et al., bioRxiv - Molecular Biology 2024

  Quote: ... and mCherry (rabbit, Abcam, ab167453, 1:4000). Detection was performed using HRP-conjugated secondary antibodies ...
• Comparative evaluation of cell-based assay technologies for scoring drug-induced condensation of SARS-CoV-2 nucleocapsid protein

  Rui Tong Quek, et al., bioRxiv - Molecular Biology 2024

  Quote: ... + 2% normal goat serum (Abcam ab7481) at room temperature for 1h ...
• The PHD3-FOXO3 axis modulates the interferon type I response in microglia aggravating Alzheimer’s disease progression

  Manuel A. Sanchez-Garcia, et al., bioRxiv - Neuroscience 2024

  Quote: ... and anti-β-actin (Abcam, 1:5,000). HRP-conjugated anti-rabbit (1:10,000 ...
• Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in Escherichia coli

  Belen Sotomayor, et al., bioRxiv - Synthetic Biology 2024

  Quote: ... The following antibodies were used for immunoblotting: polyhistidine (6x-His) tag-specific polyclonal antibody (1:5000 dilution; Abcam, Cat # ab1187); F(ab’)2-goat anti-human IgG (H+L ...
• Effects of intergenerational transmission of small intestinal bacteria cultured from stunted Bangladeshi children with enteropathy

  Kali M. Pruss, et al., bioRxiv - Systems Biology 2024

  Quote: ... 5% donkey serum and 0.1% Triton-X (antibody buffer) for 30 minutes at room temperature and treated with primary antibodies (Ki67 anti-Rabbit, 1:300, Abcam AB16667 ...
• Scalable cell-free production of active T7 RNA polymerase

  Ryan N. Rezvani, et al., bioRxiv - Synthetic Biology 2024

  Quote: ... The gels were then stained with InstantBlue (AbCam, Waltham, Massachusetts, USA) for 1 hour shaking at room temperature ...
• Epstein-Barr viral product-containing exosome facilitates LIF-associated immunosuppressive polarization of macrophage in nasopharyngeal carcinoma

  Tzu-Tung Liu, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Primary antibodies against the following proteins were applied in IHC or ICC: LIF (Abcam, ab135629, 1:80), LMP1 (Kerafast ...
• Epstein-Barr viral product-containing exosome facilitates LIF-associated immunosuppressive polarization of macrophage in nasopharyngeal carcinoma

  Tzu-Tung Liu, et al., bioRxiv - Cancer Biology 2024

  Quote: ... iNOS (Abcam, ab178945, RRID: AB_2861417, 1:25)
• Epstein-Barr viral product-containing exosome facilitates LIF-associated immunosuppressive polarization of macrophage in nasopharyngeal carcinoma

  Tzu-Tung Liu, et al., bioRxiv - Cancer Biology 2024

  Quote: ... EBV early antigen D (EaD; Abcam, ab30541, RRID: AB_732194, 1:150), CD68 (Abcam ...
• S100a4⁺ alveolar macrophages accelerate the progression of precancerous atypical adenomatous hyperplasia by promoting fatty acid metabolism

  Hong Huang, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Immunoblotting was performed with the following primary antibodies: S100a4 (Abcam), β-actin (Cell Signaling Technology) ...
• Catalytic inhibition of KAT6/KAT7 enhances the efficacy and overcomes primary and acquired resistance to Menin inhibitors in MLL leukaemia

  Shellaina J. V. Gordon, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Total H4 (Abcam, ab31830, RRID:AB_1209246, 1:1000), anti-rabbit H3K14ac (Abcam ...
• 3D Printing of Multiscale Scaffolds with Microtopography for Guiding Tissue Organization and Regeneration

  Rao Fu, et al., bioRxiv - Bioengineering 2024

  Quote: ... HASMCs were incubated in sequence with Recombinant Anti-alpha smooth muscle Actin primary antibody (0.572 μg/ml; ab133567; Abcam Inc., MA) (α-SMA ...
• 3D Printing of Multiscale Scaffolds with Microtopography for Guiding Tissue Organization and Regeneration

  Rao Fu, et al., bioRxiv - Bioengineering 2024

  Quote: ... and HUVECs were incubated with Anti-VE Cadherin (or CD144) primary antibody (3.33 μg/ml; ab33168; Abcam Inc.) overnight at 4 °C ...
• Design of an Artificial Natural Killer Cell Mimicking System to Target Tumour Cells

  Vaishali Chugh, et al., bioRxiv - Bioengineering 2024

  Quote: ... Most of the primary antibodies (anti-NKp30, anti-CD226, anti-CD11a, Anti-NKG2D) were purchased from Abcam. Anti-CD56 primary antibody and all the secondary antibodies (HRP- goat anti-rabbit and HRP-goat anti-mouse ...
• Unveiling the potential of neuron-targeted dendriplexes for siRNA delivery using a PNS-CNS-on-Chip

  Ana P. Spencer, et al., bioRxiv - Bioengineering 2024

  Quote: ... rabbit anti-oligodendrocyte transcription factor 2 (Olig2, 1: 100 dilution, Abcam), rabbit anti-ionized calcium-binding adapter molecule 1 (IBA1 ...
• HMGB1 mediates macrophage recruitment and regional intervertebral disc tissue functional and mechanical property changes following injury

  Kevin G. Burt, et al., bioRxiv - Bioengineering 2024

  Quote: ... sections were incubated for 1 hr at room temperature with secondary fluorescent antibodies for goat anti-rabbit Alexa Fluor (AF) 488 (Abcam, AB150081, 1:500) or goat anti-rat AF595 (Abcam ...
• HMGB1 mediates macrophage recruitment and regional intervertebral disc tissue functional and mechanical property changes following injury

  Kevin G. Burt, et al., bioRxiv - Bioengineering 2024

  Quote: ... or goat anti-rat AF595 (Abcam, AB150160, 1:500). Sections were mounted with VECTASHEILD DAPI anti-fade mounting medium (Vector ...
• Effect of quantified cranial osteopathic manipulation on wild type and transgenic rat models of Alzheimer’s disease

  De’Yana Hines, et al., bioRxiv - Bioengineering 2024

  Quote: ... Monoclonal antibodiy recognizing the N-terminal (Abcam, cat#ab201060) of Aβ42 peptide was used along with Antibodies recognizing AQP4 (DIF8E ...
• Broad-spectrum immune suppression encoded in self-amplifying RNA enables non-cytotoxic, non-immunostimulatory, reversible transgene expression

  Tony K.Y Lim, et al., bioRxiv - Bioengineering 2024

  Quote: ... Phospho-eIF4E (S209) rabbit monoclonal (1:200, #ab76256, Abcam), eIF4E mouse monoclonal (5D11 ...
• Intraflagellar transport protein IFT172 contains a C-terminal ubiquitin-binding U-box-like domain involved in ciliary signaling

  Nevin K. Zacharia, et al., bioRxiv - Biochemistry 2024

  Quote: ... 2.5 μM of the specified E2 enzyme (Abcam #ab139472), 5 μM ubiquitin (R&D Systems #U-100H-10M ...
• Intraflagellar transport protein IFT172 contains a C-terminal ubiquitin-binding U-box-like domain involved in ciliary signaling

  Nevin K. Zacharia, et al., bioRxiv - Biochemistry 2024

  Quote: ... The reactions were initiated by addition of 5 mM ATP and carried out in ubiquitination buffer (Abcam #ab139472) supplemented with 5 mM MgCl2 ...
• Ionizable networks mediate pH-dependent allostery in SH2 signaling proteins

  Papa Kobina Van Dyck, et al., bioRxiv - Biochemistry 2024

  Quote: ... The following antibodies were used for primary incubations: SHP2(abcam, ab76285 ...
• An ALPK3 truncation variant causing autosomal dominant hypertrophic cardiomyopathy is partially rescued by mavacamten

  Lisa Leinhos, et al., bioRxiv - Genetics 2024

  Quote: ... anti-α-actinin2 antibody (1:50, EP2529Y, abcam, rabbit) was used ...
• Subtelomere-specific condensed chromatin is regulated by three different histone modifications

  Miho Osaki, et al., bioRxiv - Genetics 2024

  Quote: ... andti-H4-K12ace (ab46983; Abcam), anti-Flag (F3165 ...
• Transposable elements drive regulatory and functional innovation of F-box genes

  Miguel Vasconcelos Almeida, et al., bioRxiv - Evolutionary Biology 2024

  Quote: ... Gels were stained with InstantBlue Coomassie stain (Abcam, #ab119211) and imaged.
• Single-cell transcriptomics reveal differences between chorionic and basal plate cytotrophoblasts and trophoblast stem cells

  Robert Morey, et al., bioRxiv - Developmental Biology 2024

  Quote: ... Samples were stained with CONFIRM® anti-EGFR (5B7) Rabbit Monoclonal Primary Antibody (Ventana 790-4347, 1:20 dilution) and anti-HLA-G (4H48) mouse primary antibody (Abcam ab52455, 1:1000 dilution) for 2 hours followed by secondary staining for 1h with Alexa Fluor anti-rabbit-647 (Life Technologies ...
• Single-cell transcriptomics reveal differences between chorionic and basal plate cytotrophoblasts and trophoblast stem cells

  Robert Morey, et al., bioRxiv - Developmental Biology 2024

  Quote: ... and rabbit anti-ITGA5 (1.1ug/ml, Abcam, ab150361) and incubated at 4°C overnight ...
• Calcium dynamics tune developmental tempo to generate evolutionarily divergent axon tract lengths

  Feline W. Lindhout, et al., bioRxiv - Developmental Biology 2024

  Quote: ... GFAP (Abcam, ab7260), Homer1 (Synaptic systems ...
• Unbiased functional genetic screens reveal essential RNA modifications in human cancer and drug resistance

  Cornelius Pauli, et al., bioRxiv - Cell Biology 2024

  Quote: ... The following primary antibodies were used: anti-TRMT5 (1:1000, ab259986, Abcam) and anti-GAPDH (1:2000 ...
• Secreted exosomes induce filopodia formation

  Caitlin McAtee, et al., bioRxiv - Cell Biology 2024

  Quote: ... Co-staining was done using mouse anti-CD63 (Abcam ab8219) and goat anti-mouse Alexa Fluor 546 ...
• Protrudin acts at ER-endosome contacts to promote KIF5-mediated endosomal fission and endosome-to-Golgi transport

  Julia Kleniuk, et al., bioRxiv - Cell Biology 2024

  Quote: ... mouse monoclonal anti-MAP2 [AP-20] (Abcam, ab11268), rabbit polyclonal anti- Beta-III tubulin (Abcam ...
• Protrudin acts at ER-endosome contacts to promote KIF5-mediated endosomal fission and endosome-to-Golgi transport

  Julia Kleniuk, et al., bioRxiv - Cell Biology 2024

  Quote: ... mouse monoclonal anti-M6PR (cation independent) [2G11] (Abcam, ab2733), rabbit monoclonal anti-GAPDH [14C10] (Cell Signalling Technology ...
• Age-dependent Changes in a Chaperone Complex in the Mouse Heart

  Purnima Devaki Bhat, et al., bioRxiv - Cell Biology 2024

  Quote: ... and Myosin (1:1,000, Abcam ab50967).The primary antibody solutions were removed ...
• The role of disease state in confined migration

  Annika Meid, bioRxiv - Cell Biology 2024

  Quote: ... Cells were stained for CHMP4B (1:100, rabbit, Abcam, ab105767) and Lamin A/C (1:100 ...
• A human mitochondrial isoform of TRPV1 regulates intracellular Ca²⁺ simultaneously with mitochondrial thermolysis

  Florian Beignon, et al., bioRxiv - Cell Biology 2024

  Quote: ... GRP75 (ab2799, Abcam) dilution 1/2000 ...
• A human mitochondrial isoform of TRPV1 regulates intracellular Ca²⁺ simultaneously with mitochondrial thermolysis

  Florian Beignon, et al., bioRxiv - Cell Biology 2024

  Quote: ... TOM20 (ab78547, Abcam) dilution 1/2000 ...
• A human mitochondrial isoform of TRPV1 regulates intracellular Ca²⁺ simultaneously with mitochondrial thermolysis

  Florian Beignon, et al., bioRxiv - Cell Biology 2024

  Quote: ... VDAC (ab14734, Abcam) dilution 1/2000 ...
• A human mitochondrial isoform of TRPV1 regulates intracellular Ca²⁺ simultaneously with mitochondrial thermolysis

  Florian Beignon, et al., bioRxiv - Cell Biology 2024

  Quote: ... α-tubuline (ab52866, Abcam) dilution 1/2000 ...
• A human mitochondrial isoform of TRPV1 regulates intracellular Ca²⁺ simultaneously with mitochondrial thermolysis

  Florian Beignon, et al., bioRxiv - Cell Biology 2024

  Quote: ... TIM23 (ab116329, Abcam) dilution 1/2000 ...
• Plasminogen Activator Inhibitor-1 is internalized by endothelial cells via macropinocytosis to evade degradation

  Molly McAdow, et al., bioRxiv - Cell Biology 2024

  Quote: ... CDC42 (Abcam), Rac1 (EMD Millipore ...
• Plasminogen Activator Inhibitor-1 is internalized by endothelial cells via macropinocytosis to evade degradation

  Molly McAdow, et al., bioRxiv - Cell Biology 2024

  Quote: ... Dynamin 2 (Abcam), CDC42 (Abcam) ...
• Annulate Lamellae biogenesis is essential for nuclear pore function

  Junyan Lin, et al., bioRxiv - Cell Biology 2024

  Quote: ... rabbit polyclonal anti-RanBP2 (Abcam, ab64276), rabbit polyclonal anti-Nup214 (Abcam ...
• Annulate Lamellae biogenesis is essential for nuclear pore function

  Junyan Lin, et al., bioRxiv - Cell Biology 2024

  Quote: ... rabbit polyclonal anti-Nup214 (Abcam, ab70497), rabbit polyclonal anti-Nup85 (Bethyl ...
• The nucleocapsid (N) proteins of different human coronaviruses demonstrate a variable capacity to induce the formation of cytoplasmic condensates

  Maria A. Tikhomirova, et al., bioRxiv - Cell Biology 2024

  Quote: ... Donkey Anti-Rabbit IgG AlexaFluor 568 (Abcam, Cat. No. Ab175692) were used.
• Reconstitution of BNIP3/NIX-mediated autophagy reveals two pathways and hierarchical flexibility of the initiation machinery

  Elias Adriaenssens, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-WIPI4 (Abcam Cat# ab168532, RRID:AB_3101989), anti-PPTC7 (1:500 ...
• A remarkable degree of conformance between the visual streak of the Mongolian gerbil and the human central retina

  Alexander Günter, et al., bioRxiv - Cell Biology 2024

  Quote: ... and anti-MERTK (ab95925; 1:100; Abcam). The use of a detergent in the blocking solution allowed for the visualization of intracellular MERTK-labelled phagosomes ...
• Reconstitution of BNIP3/NIX-mediated autophagy reveals two pathways and hierarchical flexibility of the initiation machinery

  Elias Adriaenssens, et al., bioRxiv - Cell Biology 2024

  Quote: ... anti-PPTC7 (1:500, Abcam Cat# ab122548, RRID:AB_11127117).
• ApoE4 disrupts intracellular trafficking and iron homeostasis in an improved iPSC-based model of human brain endothelial cells

  Luisa Bell, et al., bioRxiv - Cell Biology 2024

  Quote: ... Iron chelator PIH at 10 µM (ab145871, abcam) was applied to half of the wells for 10 min ...
• Epigenomic manipulation reveals the relationship between locus specific chromatin dynamics and gene expression

  Vrinda Venu, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and H3K27ac (Abcam, Cat. #ab4729). ChIP antibodies (4-5 µg ...
• Blood-based detection of MMP11 as a marker of prostate cancer progression regulated by the ALDH1A1-TGF-β1 signaling mechanism

  Ielizaveta Gorodetska, et al., bioRxiv - Cancer Biology 2024

  Quote: ... human MMP11 was measured using the MMP11 ELISA Kit (Antibodies online, ABIN6574149) and TGF-β1 by TGF beta 1 ELISA Kit (Abcam, ab108912, United Kingdom) according to manufacturers’ instructions within pre-diluted plasma aliquots (1:2).