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• A cereblon (CRBN) molecular glue degrader of NIMA-related kinase 7 (NEK7) reveals a context-dependent role in NLRP3 inflammasome activation

  Aude Sylvain, et al., bioRxiv - Immunology 2024

  Quote: ... 10 µL of anti-NEK7 (Abcam, 133514, 1:500) and anti-actin antibodies (Cell Signaling Technology ...
• A cereblon (CRBN) molecular glue degrader of NIMA-related kinase 7 (NEK7) reveals a context-dependent role in NLRP3 inflammasome activation

  Aude Sylvain, et al., bioRxiv - Immunology 2024

  Quote: ... Parallel samples were analyzed by western blotting using the following antibodies at 1:1000 dilution: rabbit polyclonal antibodies against STIL (ab89314; Abcam), NEK7 (3057S ...
• ENaC contributes to macrophage dysfunction in cystic fibrosis

  John Moran, et al., bioRxiv - Immunology 2024

  Quote: ... anti-SCNN1γ (abcam ab3468), Alexa Fluor 488 FITC dye (Thermo Fisher) ...
• ENaC contributes to macrophage dysfunction in cystic fibrosis

  John Moran, et al., bioRxiv - Immunology 2024

  Quote: ... anti-SCNN1Δ (abcam, ab196737), anti-SCNN1γ (abcam ab3468) ...
• ENaC contributes to macrophage dysfunction in cystic fibrosis

  John Moran, et al., bioRxiv - Immunology 2024

  Quote: ... anti-SCNN1β (abcam, ab28668), anti-SCNN1Δ (abcam ...
• Non-canonical Wnt signaling triggered by WNT2B drives adrenal aldosterone production

  Kleiton S. Borges, et al., bioRxiv - Genetics 2024

  Quote: ... Rabbit anti-Lef1 (Abcam, ab137872), Mouse anti-NF2R2 (R&D ...
• Distinct functions of PAXX and MRI during chromosomal end joining

  Metztli Cisneros-Aguirre, et al., bioRxiv - Genetics 2024

  Quote: ... MLH1 (Abcam ab92312), ACTIN (Sigma A2066) ...
• Distinct functions of PAXX and MRI during chromosomal end joining

  Metztli Cisneros-Aguirre, et al., bioRxiv - Genetics 2024

  Quote: ... Blots were probed with antibodies for PAXX (Abcam ab126353), FLAG-HRP (Sigma A8592) ...
• Distinct functions of PAXX and MRI during chromosomal end joining

  Metztli Cisneros-Aguirre, et al., bioRxiv - Genetics 2024

  Quote: ... and HRP goat anti-rabbit (Abcam ab205718). ECL reagent (Amersham Biosciences ...
• Distinct functions of PAXX and MRI during chromosomal end joining

  Metztli Cisneros-Aguirre, et al., bioRxiv - Genetics 2024

  Quote: ... HRP goat anti-mouse (Abcam ab205719), and HRP goat anti-rabbit (Abcam ab205718) ...
• Conserved sites on the influenza H1 and H3 hemagglutinin recognized by human antibodies

  Daniel P. Maurer, et al., bioRxiv - Immunology 2024

  Quote: ... Anti-human IgG HRP conjugate (Abcam) was then added (1 hour ...
• Low Dose Radiation by Radiopharmaceutical Therapy Enhances GD2 TRAC-CAR T Cells Efficacy in Localized Neuroblastoma

  Quaovi H. Sodji, et al., bioRxiv - Immunology 2024

  Quote: ... labelled with APC via the APC Conjugation Kit Lightning-Link (cat. no. ab201807; Abcam). Other fluorophore-conjugated antibodies used include anti-GD2-PE-Dazzle594 (cat ...
• The Shigella flexneri effector IpaH1.4 facilitates RNF213 degradation and protects cytosolic bacteria against interferon-induced ubiquitylation

  Luz Saavedra-Sanchez, et al., bioRxiv - Microbiology 2024

  Quote: ... rabbit monoclonal anti K27 (Abcam ab181537, 1:100), mouse monoclonal anti-Strep (Genscript A01732 ...
• LPS binding caspase activation and recruitment domains (CARDs) are bipartite lipid binding modules

  Anh B. Cao, et al., bioRxiv - Immunology 2024

  Quote: ... Corresponding protein bands were visualized using InstantBlue® Coomassie Protein Stain (abcam, ISB1L) and rinsed with distilled water ...
• 0JAK Inhibitor Withdrawal Causes a Transient Proinflammatory Cascade: A Potential Mechanism for Major Adverse Cardiac Events

  Ilya Gurevic, et al., bioRxiv - Immunology 2024

  Quote: ... others were from Abcam: total STAT2 (#32367) ...
• Nerve-associated macrophages control adipose homeostasis across lifespan and restrain age-related inflammation

  Elsie Gonzalez-Hurtado, et al., bioRxiv - Immunology 2024

  Quote: ... Atp5a (MitoProfile total OXPHOS, Abcam; 110413). HRP-conjugated anti-mouse (Invitrogen ...
• Nerve-associated macrophages control adipose homeostasis across lifespan and restrain age-related inflammation

  Elsie Gonzalez-Hurtado, et al., bioRxiv - Immunology 2024

  Quote: ... MAOA (Abcam; ab126751), β-Actin (Cell Signaling ...
• Dynamic epigenomic landscape of carbon-concentrating mechanisms in the model industrial oleaginous microalga Nannochloropsis oceanica

  Yanhai Gong, et al., bioRxiv - Genomics 2024

  Quote: ... anti-H3K4me2 (Abcam, UK; ab7766) or anti-crotonyllysine (PTM BioLab ...
• Efficient multiplex non-viral engineering and expansion of polyclonal γδ CAR-T cells for immunotherapy

  Jacob Bridge, et al., bioRxiv - Immunology 2024

  Quote: ... Blood samples were analyzed by flow cytometry and an IL-15 ELISA kit (abcam) was used to measure peripheral blood IL-15 concentration ...
• Type III interferons suppress influenza A virus infection independently of STAT activation by triggering cell death

  Wiktor Prus, et al., bioRxiv - Immunology 2024

  Quote: ... anti- influenza A virus (catalog number ab20841; Abcam) (1:1,000) ...
• Chromatin-dependent motif syntax defines differentiation trajectories

  Sevi Durdu, et al., bioRxiv - Genomics 2024

  Quote: Primary antibodies for immunofluorescence: anti-Nanog (1:100, abcam, EPR20694), anti-BetaIII Tubulin (1:1000 ...
• Type III interferons suppress influenza A virus infection independently of STAT activation by triggering cell death

  Wiktor Prus, et al., bioRxiv - Immunology 2024

  Quote: ... anti-respiratory syncytial virus (catalog number ab20745; Abcam) (1:1,000) ...
• Type III interferons suppress influenza A virus infection independently of STAT activation by triggering cell death

  Wiktor Prus, et al., bioRxiv - Immunology 2024

  Quote: ... anti-influenza A virus nucleoprotein (clone C43) (catalog number ab128193; Abcam) (1:1,000) ...
• Reshaping the tumor microenvironment by degrading glycoimmune checkpoints Siglec-7 and -9

  Chao Wang, et al., bioRxiv - Immunology 2024

  Quote: ... AF647 Dnk anti-rabbit (Abcam) and AF405 Phalloidin (Invitrogen ...
• Reshaping the tumor microenvironment by degrading glycoimmune checkpoints Siglec-7 and -9

  Chao Wang, et al., bioRxiv - Immunology 2024

  Quote: ... followed by staining with AF555 Dnk anti-goat (Abcam) and DAPI ...
• Reshaping the tumor microenvironment by degrading glycoimmune checkpoints Siglec-7 and -9

  Chao Wang, et al., bioRxiv - Immunology 2024

  Quote: ... and incubated with primary anti-CI-M6PR (Abcam, clone 2G11) on ice for 30 min ...
• Reshaping the tumor microenvironment by degrading glycoimmune checkpoints Siglec-7 and -9

  Chao Wang, et al., bioRxiv - Immunology 2024

  Quote: ... followed by staining with AF555 Dnk anti-goat (Abcam), AF647 Dnk anti-rabbit (Abcam ...
• Reshaping the tumor microenvironment by degrading glycoimmune checkpoints Siglec-7 and -9

  Chao Wang, et al., bioRxiv - Immunology 2024

  Quote: ... Cells were washed with PBST buffer and incubated with secondary AF555 Dnk anti-goat and AF647 Dnk anti-Rb (Abcam) diluted in 4% FBS in PBST containing 5 μg/mL hIgG at r.t ...
• Reshaping the tumor microenvironment by degrading glycoimmune checkpoints Siglec-7 and -9

  Chao Wang, et al., bioRxiv - Immunology 2024

  Quote: ... The membrane was blocked and probed with the following primary antibodies: anti-pCD3ζ Y142 (Abcam, Cat# ab68235), anti-CD3ζ (Biolegend ...
• Membrane Expression Enhances Folding, Multimeric Structure Formation, and Immunogenicity of Viral Capsid Proteins

  Junru Cui, et al., bioRxiv - Immunology 2024

  Quote: ... cells were incubated with mouse anti-P72 antibody or mouse anti-HA antibody and AF488 conjugated anti-mouse secondary antibody (Abcam), and images were taken with a fluorescence microscope (Olympus FV1200 Laser Scanning Confocal Microscope) ...
• Membrane Expression Enhances Folding, Multimeric Structure Formation, and Immunogenicity of Viral Capsid Proteins

  Junru Cui, et al., bioRxiv - Immunology 2024

  Quote: ... cells were trypsinized and incubated with mouse anti-P72 antibody or mouse anti-HA antibody and AF488 conjugated anti-mouse secondary antibody (Abcam). Data was acquired with LSR Fortessa HTS-2 (BD Biosciences ...
• Multi-modal profiling identifies CD4+CXCR5+PD-1- Tfh cells as prognostic and predictive biomarkers for response to R-CHOP therapy in human DLBCL

  Sisi Yu, et al., bioRxiv - Immunology 2024

  Quote: ... anti-CD4 (ab133616, Abcam, US)/TSA 670 ...
• Bronchopulmonary Dysplasia with Pulmonary Hypertension Associates with Loss of Semaphorin Signaling and Functional Decrease in FOXF1 Expression

  Shawyon P. Shirazi, et al., bioRxiv - Genomics 2024

  Quote: ... anti-ERG1 (1:100, Abcam ab214341) and the following secondary antibodies used ...
• Monocyte-derived cells but not Microglia cause Oxidative Tissue Damage in Neuroinflammation

  Juan Villar-Vesga, et al., bioRxiv - Immunology 2024

  Quote: ... and Actin (ab49900, Abcam, 1:500) overnight at 4°C and secondary antibody for 1h at RT ...
• Metabolomic Profiling Reveals Potential of Fatty Acids as Regulators of Stem-like Exhausted CD8 T Cells During Chronic Viral Infection

  Katelynn R Kazane, et al., bioRxiv - Immunology 2024

  Quote: ... Plasma samples were analyzed with the Free Fatty Acid Assay Kit (Abcam) per manufacturer’s instructions ...
• IL-1β-driven NF-κB transcription of ACE2 as a Mechanism of Macrophage Infection by SARS-CoV-2

  Cadence Lee, et al., bioRxiv - Immunology 2024

  Quote: ... JSH23 (Abcam, ab144824).
• G-CSF and IL-6 drive myeloid dysregulation during severe viral infection

  Kimberly Kajihara, et al., bioRxiv - Immunology 2024

  Quote: ... Enzyme-linked immunosorbent assay (ELISA) kits were used to assay mouse albumin (Abcam) and mouse sRAGE (R&D).
• Single-cell and spatiotemporal transcriptomic profiling of brain immune infiltration following Venezuelan equine encephalitis virus infection

  Margarita V. Rangel, et al., bioRxiv - Immunology 2024

  Quote: ... sections were incubated with Ly6c primary antibody (ab314120, Abcam) and secondary antibody (ab6720 ...
• Single-cell and spatiotemporal transcriptomic profiling of brain immune infiltration following Venezuelan equine encephalitis virus infection

  Margarita V. Rangel, et al., bioRxiv - Immunology 2024

  Quote: ... and hydrogen peroxide (ab64218, Abcam) was used to block endogenous peroxidase ...
• Single-cell and spatiotemporal transcriptomic profiling of brain immune infiltration following Venezuelan equine encephalitis virus infection

  Margarita V. Rangel, et al., bioRxiv - Immunology 2024

  Quote: ... and secondary antibody (ab6720, Abcam). 3,3′-diaminobenzidine (DAB ...
• Abnormal eNK cells contribute to endometrial fibrosis in intrauterine adhesions patients

  Zhenhua Zhou, et al., bioRxiv - Immunology 2024

  Quote: ... Nuclei were stained with DAPI (Abcam). The ESCs were washed with PBS for three times ...
• RNA-binding proteins control the G2-M checkpoint of the germinal centre B cell

  Fiamma Salerno, et al., bioRxiv - Immunology 2024

  Quote: ... anti-cyclin E2 (E142-Abcam), anti-cyclin B1 (D5C10 - Cell Signalling) ...
• Prohibitin 2 is a Key Regulator of T Cell Proliferation and Effector Functions

  Johannes F. Vogt, et al., bioRxiv - Immunology 2024

  Quote: Stimulated naïve CD4 T cells were incubated with 25nM/ml TMRE (Abcam #AB113852) for membrane potential and 100nM Mitotracker Green FM (Thermo Fischer Scientific M7514 ...
• T3SS translocon induces pyroptosis by direct interaction with NLRC4/NAIP inflammasome

  Yan Zhao, et al., bioRxiv - Immunology 2024

  Quote: ... the cells were treated with 50 μM cytochalasin B (ab143482, Abcam) or 10 μM cytochalasin D (PHZ1063 ...
• T3SS translocon induces pyroptosis by direct interaction with NLRC4/NAIP inflammasome

  Yan Zhao, et al., bioRxiv - Immunology 2024

  Quote: ... anti-6×His tag mAb (ab213204, Abcam), flag-tag rabbit mAb (AE063 ...
• T3SS translocon induces pyroptosis by direct interaction with NLRC4/NAIP inflammasome

  Yan Zhao, et al., bioRxiv - Immunology 2024

  Quote: ... goat anti-rabbit IgG H&L (HRP) (ab97051, Abcam). For immunoprecipitation ...
• 2-D organoids demonstrate specificity in the interactions of parasitic nematodes and their secreted products at the basal or apical intestinal epithelium

  Ruby White, et al., bioRxiv - Immunology 2024

  Quote: ... α-DCAMKL1 (Abcam, UK, ab31704), α-Villin (1:100 ...
• 2-D organoids demonstrate specificity in the interactions of parasitic nematodes and their secreted products at the basal or apical intestinal epithelium

  Ruby White, et al., bioRxiv - Immunology 2024

  Quote: ... α-Villin (1:100, Abcam, ab130751), α-Chromogranin-A (1:50 ...
• 2-D organoids demonstrate specificity in the interactions of parasitic nematodes and their secreted products at the basal or apical intestinal epithelium

  Ruby White, et al., bioRxiv - Immunology 2024

  Quote: ... α-Chromogranin-A (1:50, Abcam, ab15160), α-Ki67 (1:250 ...
• 2-D organoids demonstrate specificity in the interactions of parasitic nematodes and their secreted products at the basal or apical intestinal epithelium

  Ruby White, et al., bioRxiv - Immunology 2024

  Quote: ... and Phalloidin-iFluor 594 (1:2000, Abcam) were added for 1hr at RT covered from light ...