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Citations for Promega :
201 - 250 of 2350 citations for mmu mir 212 3p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin-labelled or Fluorescein-labelled mRNA probes were either prepared as PCR probes with primers listed in Table S4 using the Kit GoTaq green master mix (Promega) or as described (Orgeur et al. ...
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bioRxiv - Immunology 2024Quote: ... miiuy MDA5 gene was amplified using PCR with the gene-specific primers and ligated into a pmir-GLO luciferase reporter vector (Promega). Meanwhile ...
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bioRxiv - Microbiology 2024Quote: ... named rpsL*) was amplified by PCR with primers BlD-LLcfusARpsL/BlD-LLldacARpsL and cloned into the pGEM-T Easy vector (Promega), yielding plasmid pGEM-rpsL* ...
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bioRxiv - Neuroscience 2021Quote: RT-qPCR was conducted using one-step RT-qPCR kit (Promega, A6020), according to manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR reactions using the GoTaq 1-Step RT-qPCR kit (Promega) were set-up to 10 µl final reaction volumes according to the manufacturer’s instructions containing 250 nM of each receptors primer pairs and 10 ng of RNA ...
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bioRxiv - Molecular Biology 2020Quote: ... and real-time polymerase chain reaction (RT-PCR) conducted using the Techne Prime Pro thermal cycler with GoTaq qPCR Master Mix (Promega). RT-qPCR reactions were carried out in 10 μL volumes containing 3.5 mM MgCl2 ...
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bioRxiv - Bioengineering 2021Quote: ... Ligations of the digested plasmid backbones and PCR products occurred for 5-10 minutes at RT using T4 DNA ligase (Promega) prior to transformation into NEB® Turbo Competent E ...
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bioRxiv - Physiology 2019Quote: The expression profile of PKC isoforms in UMR106 cells was studied by RT-PCR using the GoTaq Green Master Mix (Promega) and the primers listed in Table 1 ...
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bioRxiv - Plant Biology 2019Quote: Semi-quantitative RT-PCR was performed on RNA isolated of 10 days-old seedlings using the total RNA isolation system (Promega). Reverse transcription was performed on 300 ng of total RNA using the iScript advanced kit (BioRad) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... QRT-PCR reactions were performed by using the GoTaq®1-Step RT-qPCR System (Promega Scientific, Madison, WI, USA) with Bio-Rad CFX Connect (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2019Quote: ... or AMV RT (Promega) as described in manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2019Quote: The wild type and mutant promoter of miR-146a-5p were constructed into pGL3 vector (Promega). The pGL3-WT or pGL3-mut were co-transfected with pRL-TK using lipofectamine® 3000 ...
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bioRxiv - Immunology 2021Quote: pCIneo Precursor miR-146a (pmiR-146a) and HA-HuR plasmids were transfected using Fugene HD (Promega) for RAW264.7 cells as described previously (Goswami et.al ...
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bioRxiv - Developmental Biology 2021Quote: ... the adar cDNA sequence was PCR-amplified using the primer pair 5’-CCTGTCTTTGATACTGTCGTG-3’ and 5’-TCCCGAAGCCACAGATTCAC-3’ and cloned into p-GEMT vector (Promega, USA). For the rescue experiment ...
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bioRxiv - Genomics 2022Quote: ... The efficiency and specificity of the designed primers was tested through PCR using the GoTaq Green Master kit (Promega, Madison, USA) with thermal cycling conditions of 2 min at 95 °C ...
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bioRxiv - Microbiology 2024Quote: ... 1522bp using primers 5’-AAGGTACCTGAGGCTGGAGAGATGGCC-3’ and 3’-TAAAAGCTTCACCGGACTGGGCTAGTTCAG-5’ were PCR amplified and cloned in promoterless PGL3 enhancer empty vector (Promega, E1771) at the upstream of luciferase gene ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using the GoTaq 1-step RT-qPCR system (Promega). Knockdowns were verified using primers described in Table I ...
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bioRxiv - Cell Biology 2020Quote: ... Random primer (Promega, Madison, Wisconsin) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and random primers (Promega C1181). Real-time PCR was performed using Power SYBR Green PCR Master Mix (Applied Biosystems 4367659) ...
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bioRxiv - Molecular Biology 2021Quote: ... and Oligo dT primers (Promega). RT-qPCR was performed using Power SYBR Green (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... 0.5 µg random primers (Promega) and GoScript™ reverse transcriptase (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... 100ng-1μg of RNA was used to generate cDNA using the MMLV reverse transcriptase by Promega and oligo-dT primers (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... using random primers (Promega, C1181). Expression levels of mRNAs encoding Oct3/4 ...
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bioRxiv - Neuroscience 2020Quote: ... and Oligo (dT) primer (Promega), and the following heat cycle was used for the reverse transcription ...
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bioRxiv - Cell Biology 2021Quote: ... and Oligo dT15 primers (Promega) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... by using random primers (Promega). Quantitative PCR was performed using the FastStart Essential DNA Green Master (Roche ...
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bioRxiv - Immunology 2020Quote: ... with random hexamer primers (Promega). Total RNA yield varied between patients and between 6 ng-100 ng was used for each of the isotype PCRs using IGHV FR1 primers based on the BIOMED-2 design (van Dongen et al. ...
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bioRxiv - Molecular Biology 2020Quote: ... Random Primers Kit (Promega, A2800) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... 100 ng Random Primers (Promega), 400 ng Anchored Oligo (dT)20 Primer (Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: ... and random hexamer primers (Promega).
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bioRxiv - Immunology 2020Quote: ... and random hexamer primers (Promega). PCR was carried out by QuantStudio-3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... and random hexamer primer (Promega). qPCRs were run on LightCycler® 480 Instrument II (Roche ...
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bioRxiv - Genetics 2023Quote: ... 1μl of random primers (Promega), RNasin Plus RNase inhibitor (Promega) ...
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bioRxiv - Genetics 2023Quote: ... and random hexamer primers (Promega). PCR was carried out by QuantStudio-3 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2024Quote: ... with random hexamer primers (Promega) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... using oligo-dT primers (Promega). Quantitative real time PCR was performed with Fast Sybergren Master mix (In vitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... Primer concentrations for RT-qPCR reactions were initially evaluated by performing 25μL PCR reactions using Go Taq® Master Mix (Promega Corporation) with approximately 100μg of gDNA ...
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bioRxiv - Neuroscience 2020Quote: ... RT-PCR reactions were performed using 20 ng of cDNA of WT2 and NBS8 organoids using GoTaq® DNA Polymerase (Promega). Total RNA from commercially human fetal and adult brain (BioChain® ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR (qPCR) reactions were conducted in a 10-µL total volume using a 2× GoTaq® qPCR Master Mix (Promega) and run on an ABI 7500 qPCR thermocycler (Applied Biosystems) ...
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bioRxiv - Neuroscience 2021Quote: ... reverse transcription of viral RNA followed by quantitative PCR was performed with GoTaq® Probe 1-Step RT-qPCR System (Promega) on a 7500 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombination was verified by PCR with primers listed in Supplementary table 5 and genomic DNA isolated with the ReliaPrep™ gDNA Tissue Miniprep System (Promega, A2052) and peqGOLD Tissue DNA Mini Kit (Peqlab ...
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bioRxiv - Molecular Biology 2020Quote: ... Real Time PCR was done with unique oligonucleotide primers targeting ABCB11 gene using GoTaq® qPCR Master Mix (Promega Cat. No. A6001) following ‘manufacturer’s instructions on a Veriti Thermo Cycler from Applied Biosystems Waltham ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using the GoTaq® 1-Step RT-qPCR System (Promega). Knockdowns were verified using the primers ...
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bioRxiv - Genomics 2020Quote: ... 0.2 μL GoScript™ RT Mix for 1-Step RT-qPCR (Promega, Madison, WI), 0.75 μL primer/probe sets for either N1 ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed using the GoTaq® 1-Step RT-qPCR System (Promega) with an input of 50 ng RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... RT-qPCR was performed using the GoTaq® 1-Step RT-qPCR System (Promega) with an input of 50 ng RNA ...
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bioRxiv - Molecular Biology 2019Quote: ... (vi) BP4_DM2_H-(GoScript RT, Promega) consisted of 9 DM2 and 4 control samples ...
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bioRxiv - Molecular Biology 2019Quote: ... (v) BP3_DM1_H-(GoScript RT, Promega) consisted of 2 DM1 and 4 control samples ...
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bioRxiv - Developmental Biology 2020Quote: The coding region of her9 was amplified by RT-PCR and the cDNA was cloned into a pGEMT-easy plasmid (Promega, Madison, WI). Capped mRNA was synthesized using the mMessage T7 kit (Ambion ...
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bioRxiv - Molecular Biology 2020Quote: ... all RT mixture of samples were pooled and purified with Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI, USA) according to the manufacture’s manual except for using 80% EtOH instead of Membrane Wash Solution ...