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Citations for Promega :
151 - 200 of 7454 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... gene activity was assessed according to the supplied Luciferase Assay System Kit protocol (Promega). Luminescence detection was performed using the Microlumat LB96p device (EGG Berthold ...
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bioRxiv - Microbiology 2023Quote: ... and the 200 nM GoTaq® Probe 1-Step RT-qPCR System Kit (Promega). This kit uses GoTaq Probe qPCR Master Mix with dUTP (10 uL) ...
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bioRxiv - Biochemistry 2023Quote: ... The measurements used the Enliten ATP Assay System (Bioluminescence Detection Kit, Promega, Madison, WI). The luminescence reaction contained 5 µL of neutralized acid-soluble supernatant and 45 µL of luciferin/luciferase reagent ...
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bioRxiv - Biochemistry 2023Quote: ... and cDNA synthesis carried out using the GoScript™ Reverse Transcription System kit (Promega). Quantitative PCR was performed using a Roche LightCycler® 96 rapid thermal cycler instrument and the KAPA SYBR® FAST qPCR Master Mix for LightCycler® ...
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bioRxiv - Plant Biology 2019Quote: ... Two micrograms of total RNA were treated with RQ1 RNase-Free DNase (Promega) and processed with the ImProm-II Reverse Transcription System (Promega ...
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bioRxiv - Plant Biology 2023Quote: Two vials of trypsin/Lys-C mix (100µg, V5078, Promega, Alexandria, NSW, Australia) were dissolved into 1 mL of the resuspension buffer (50mM acetic acid ...
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bioRxiv - Pathology 2023Quote: ... we performed two overlapping PCR on the RCA products with GoTaq Flexi (Promega) according to the manufacturer’s protocol to amplify the complete genome of MSV using primers targeting MSV-G (PCR#1 ...
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bioRxiv - Molecular Biology 2023Quote: ... The assay was validated using Taq DNA polymerase from two different manufacturers (Promega Gotaq Flexi DNA polymerase or Invitrogen Taq DNA polymerase ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was isolated from cells using the ReliaPrep™ tissue system kit (Promega; Z6012). cDNA was produced from 1 μg of RNA sample using M-MLV reverse transcriptase (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... and EGFP were synthesized using the RiboMAX Large Scale RNA Production System T7 kit (Promega). Approximately 100 μg of synthesized dsAntp was injected into the second chest spiracle at the first day of Bombyx larval wandering stage ...
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bioRxiv - Systems Biology 2019Quote: ... Luciferase activity was measured 48 hours after transfection using the Luciferase Assay System kit (Promega) on a Enspire luminometer (Perkin-Elmer) ...
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bioRxiv - Genomics 2019Quote: ... using silica columns included in the SV Total RNA Isolation System extraction kit from Promega. The integrity of the obtained RNA was determined by electrophoresis in 2% agarose gel ...
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bioRxiv - Immunology 2021Quote: ... then analyzed for ATP levels using the ENLITEN ATP Assay System Bioluminescence Detection Kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2021Quote: ... and converted to cDNA using the ImProm-II Reverse Transcription System kit (Promega, Madison, USA).
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bioRxiv - Bioengineering 2022Quote: The dual luciferase assay was performed using the Dual-Luciferase Reporter Assay System kit (Promega). 1X passive lysis buffer (PLB ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Double-stranded RNAs (dsRNAs) were in vitro synthesised using the RiboMax T7 System Kit (Promega). As a control we prepared GFP dsRNA from a pGreen Lantern plasmid (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... luciferase-based reporter assays were performed using the ONE-GloTM Luciferase Assay System kit (Promega). Jurkat T cells were transfected with bacterial plasmid constructs expressing luciferase under control of the promotor region of the ligand-binding domain for PPARα ...
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bioRxiv - Microbiology 2019Quote: ... Amplicons were purified using a Wizard SV Gel and PCR Clean up System kit (Promega) and EcoRI/XhoI digested pGREG533 was gel purified using a Qiaex II kit (Qiagen) ...
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bioRxiv - Cell Biology 2019Quote: Genomic DNA (gDNA) was extracted using the ReliaPrep ™ gDNA Tissue Miniprep System kit (Promega) according to the manufacturer’s specifications ...
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bioRxiv - Genomics 2020Quote: ... RNA was extracted using a ReliaPrep™ FFPE Total RNA Miniprep System kit (Promega; Z1001) following the manufacturers recommended protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Flexicloning transfer systems (C8820 and C9320) and NanoBIT starter kit (N2015) were obtained from Promega. Anti-His antibody was from Thermo Fisher Scientific (37-2900 ...
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bioRxiv - Microbiology 2023Quote: Total cellular RNA was extracted using the Reliaprep RNA Cell Miniprep System kit (Promega; Z6012) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... luciferase-based reporter assays were performed using the ONE-GloTM Luciferase Assay System kit (Promega). COS7 cells were transfected with bacterial plasmid constructs expressing luciferase under the control of the ligand-binding domain for LXRα or LXRβ ...
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bioRxiv - Molecular Biology 2023Quote: ... Luciferase activity was measured 48 h after transfection using the Luciferase Assay System kit (Promega) on a Enspire luminometer (Perkin-Elmer) ...
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bioRxiv - Developmental Biology 2023Quote: ... and cell lysates were examined for luciferase activities with the luciferase assay system kit (Promega). The relative luciferase activities were calculated after normalization with the ß-gal activities ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluc and Rluc activities were measured using the Dual-Luciferase Reporter Assay System kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: Plasmids were extracted using Wizard® Plus SV Minipreps DNA Purification System Kit from Promega as per the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... The P450-Glo CYP1A1/1B1 assay kit and NADPH regeneration system and luciferase assay kit were purchased from Promega (Promega, Madison, WI) and utilized under the following conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell lines were authenticated using genomic DNA extracted with Puregene Gentra Kit and multiplexed using the Promega Geneprint Kit and multiplexed with a STR-based method (Promega Geneprint System). Samples were run on an Applied Biosystems 3130xl DNA analyser and the results analysed using the Applied Biosystems Genemapper v4.1 software ...
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bioRxiv - Microbiology 2020Quote: ... Two-rounds of translocation PCR was performed using GoTaq G2 Green Master Mix (Promega). In the first round PCR ...
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bioRxiv - Systems Biology 2020Quote: ... There were two positive controls: one was pGL4.10 vector (Promega, with luc2 firefly gene) with pTran promoter inserted between NheI and XhoI sites ...
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bioRxiv - Microbiology 2022Quote: ... Two μg of total RNA was treated with RQ1 DNase enzyme (Promega; Madison, WI) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Two days after transfection with 2 to 2.5 μg DNA using Fugene HD (Promega), confocal images were acquired using an FV1000 confocal laser scanning microscope (Olympus ...
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bioRxiv - Synthetic Biology 2020Quote: ... Proteins were digested with two separate and sequential aliquots of sequencing grade trypsin (Promega) of 1 μg ...
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bioRxiv - Plant Biology 2023Quote: ... Two (2) μg of total RNA were used for cDNA synthesis with MMLV (Promega) and random hexamers (Invitrogen) ...
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bioRxiv - Bioengineering 2024Quote: ... two cytocompatibility tests were performed using cell proliferation MTS assay (Promega, Cat. No. G3580). This assay involved the conversion of MTS [3-(4,5-dimethyl-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium] into formazan by living cells in a reducing environment ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... membrane integrity (CytoTox-OneTM homogeneous membrane integrity assay kit: lactate dehydrogenase (LDH release) and caspase activity/ apoptosis (Caspase-Glo® 3/7 assay system kit) (all from Promega, WI, USA), as described previously17 (see Supplementary data for detailed description).
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bioRxiv - Cell Biology 2020Quote: ... RNA was isolated from organoids and tumoroids using the ReliaPrep RNA Cell Miniprep System kit (Promega) and cDNA was prepared using the High Capacity cDNA Reverse Transcription kit (ThermoFisher ...
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bioRxiv - Evolutionary Biology 2019Quote: Dual luciferase (LUC) assay extracts were prepared using the Dual Luciferase Reporter Assay System kit (Promega). Five mg of powdered tissue was mixed with 100 mL of the passive lysis buffer (PLB ...
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bioRxiv - Cancer Biology 2021Quote: ... Mitochondria ATP content was quantified using the ENLITEN® ATP Assay System Bioluminescence Detection Kit (Promega, Wisconsin ...
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bioRxiv - Cell Biology 2021Quote: ... Retro-transcription to cDNA was performed with the ImProm-IITM Reverse Transcription System kit (Promega, A3800) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: The activity of phosphatase was determined by the Serine/Threonine Phosphatase Assay System kit from Promega Company ...
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bioRxiv - Cancer Biology 2019Quote: ... Total RNA purification was performed using the kit SV total RNA isolation system (Promega, Fitchburg, USA). Purified total RNA was quantified using NanoDrop (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Luciferase activity was measured after 48 h using the Dual-Glo Luciferase Assay System kit (Promega) and the GloMax Multi Detection System (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... using a coupled transcription-translation system containing wheat germ extract (TNT kit; Promega, Cat no. L5030), according to the manufacturer protocol.
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bioRxiv - Physiology 2021Quote: ... The concentration was measured with Quantus™ Fluorometer and the QuantiFluor® RNA System kit (Promega). The length from RNA fragments was evaluated using the Agilent Pico Kit and the Agilent 2100 BioAnalyzer (Agilent Technologies).
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bioRxiv - Developmental Biology 2021Quote: ... cells were lysed and the luciferase activities were analyzed using the Luciferase Assay System kit (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: In vitro transcription was carried out in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: ... Brazil.The GoScript reverse transcription system kit and CytoTox 96 Non-Radioactive Cytotoxicity Assay were from Promega. Brazol reagent was from LGC Biotecnologia ...
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bioRxiv - Plant Biology 2022Quote: ... The luciferase (LUC) activity was measured with the Dual-Luciferase Reporter Assay System Kit (Promega E1910) using the TriStar2 Multimode Reader LB942 (Berthold Technologies) ...