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Citations for Promega :
401 - 450 of 7454 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The presence of a 108 bp deletion in Stx6 was determined using two PCR reactions with the following primer combinations with GoTaq G2 Hot Start Polymerase (Promega): PCR 1 forward 5’-CGATCTGTGAGACTCATCGGG and reverse 5’-GGGAGTCCTAACACCACCTTC ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of the lysate were transferred into two Microtiter plates (one for Renilla and the second for Bright Glo (Promega)) ...
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bioRxiv - Cancer Biology 2023Quote: ... Testing was performed in a 96-well plate-format with 10,000 cells per well and using dose-response matrices in a 10×10 grid manner (0-10uM in two-fold dilutions) and cell viability was measured with CellTiter-Glo (Promega). The dose-response matrices were then used to compute synergy and antagonism scores using Bliss synergy modelling with Combenefit software.
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bioRxiv - Cancer Biology 2023Quote: ... Ten thousand cells per well were treated in a 96-well plate-format for 72hrs with a range of concentrations (0-50uM in a series of 20 two-fold dilutions) and cell viability was measured with CellTiter-Glo (Promega). Drug sensitivity was compared by calculating IC50 values between MM cell lines using four parameter non-linear regression models and one-way ANOVA analysis for multiple comparisons ...
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bioRxiv - Plant Biology 2023Quote: Precipitated protein was resuspended in 100mM ammonium bicarbonate (ABC) and then digested with two separate and sequential aliquots of sequencing grade trypsin (Promega) in the ratio of 1:75 trypsin to sample protein ratio overnight followed by a 3-hr digestion ...
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bioRxiv - Cancer Biology 2023Quote: ... Alkylated proteins were then cleaved using a two-step digestion: first with Endoproteinase Lys-C (ratio 1:33 enzyme: lysate, Promega) for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme ...
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bioRxiv - Microbiology 2019Quote: ... quantified with Qubit dsDNA HS Assay kit and then cloned using the pGEM®-T Easy Vector Systems (Promega, Madison, Wisconsin, US). Plasmids were isolated using the NucleoSpin Plasmid kit (Macherey-Nagel ...
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bioRxiv - Genetics 2019Quote: ... The longer band containing exon14b was then excised from the gel and purified using Zymoclean gel DNA recovery kit (Zymoresearch, Irvine, CA) and cloned using pGEM-T Easy Vector System (Promega, Madison, WI). The plasmid was used in a transformation into Subcloning Efficiency DH5α Competent Cells (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... The firefly and Renilla luciferase activity of the cells was quantified by a Dual Luciferase Reporter System Kit (E1910, Promega, WI, USA) following the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... and cleaned-up PCR product concentrations were measured using a QuantiFluor® dsDNA System assay kit on a Quantus™ Fluorometer (Promega). DNA library preparation was performed using the Ligation sequencing kit SQK-LSK309 (Oxford Nanopore Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Positive clones were cultured in liquid LB medium supplemented with kanamycin (50 μg mL−1) for extraction of plasmid DNA with the Wizard Plus SV Minipreps DNA Purification System Kit (Promega - Madison, USA). Plasmids with unique EcoRI/HindIII restriction patterns were subjected to re-transformation and phenotypic confirmation by streaking the clones in solid LB medium supplemented with kanamycin (50 μg mL−1) ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from these bacterial suspensions using a Maxwell® 16 automated nucleic acid purification system and the Maxwell® tissue DNA purification kit following the manufacturer’s instructions (Promega, UK). Purified DNA extracts were treated with RNase (New England BioLabs ...
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bioRxiv - Physiology 2020Quote: ... The transferase dUTP Nick End Labeling (TUNEL) assay was performed on cryosections of rat eyes using the TUNEL apoptosis detection kit (DeadEnd Fluorometric TUNEL System; Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and cleaned-up PCR product concentrations were measured using a QuantiFluor® dsDNA System assay kit on a Quantus™ Fluorometer (Promega). DNA library preparation was performed using the Ligation sequencing kit SQK-LSK309 (Oxford Nanopore Technologies ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA from MB cells treated with RA for 48 h was extracted using the SV Total RNA Isolation System kit (Promega, Madison, USA), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: The ATP quantification was performed using ENLITEN® ATP Assay System Bioluminiscence Detection Kit from Promega (protocol by (Yang et al., 2015)) ...
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bioRxiv - Developmental Biology 2023Quote: ... Single transformed ampicillin resistant colonies were grown in LB + Ampicillin and their DNA was extracted by the Wizard® Plus SV Minipreps DNA Purification System kit (Promega, A1460). Clones with correct insertions were evaluated by Sanger sequencing (LightRun tube ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted from roots sampled as above using the SV total RNA isolation kit system following the manufacturer’s instructions (Promega, Madison, WI, USA). cDNA was synthesized with Superscript III reverse transcriptase (Invitrogen® ...
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bioRxiv - Cell Biology 2020Quote: Renilla Luciferase Assay System (Promega) was used for measuring the production of SunTag reporters ...
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bioRxiv - Evolutionary Biology 2020Quote: ... with Quantifluor RNA system (Promega). First-strand cDNAs were synthesized from each sample ...
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bioRxiv - Neuroscience 2020Quote: ... QuantiFluor dsDNA System (Promega Corp.), and Kapa Illumina Library Quantification qPCR assays (Kapa Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... The QuantiFluor dsDNA System (Promega) was used to quantify the amount of formed double-stranded DNA ...
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bioRxiv - Microbiology 2021Quote: ... GSK3β kinase enzyme system (Promega) was used as per manufactures protocol with following modifications ...
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bioRxiv - Genetics 2022Quote: ... QuantiFluor® dsDNA System (Promega) and Kapa Illumina Library Quantification qPCR assays (Kapa Biosystems) ...
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bioRxiv - Physiology 2019Quote: ... Easy Vector Systems (Promega, Italy), a mix was prepared containing ...
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bioRxiv - Genomics 2021Quote: ... and Luciferase Assay System (Promega) for all other transfections ...
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bioRxiv - Genomics 2021Quote: ... Renilla Luciferase Assay System (Promega) for null promoter Renilla transfections ...
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bioRxiv - Evolutionary Biology 2020Quote: ... with Quantifluor dsDNA system (Promega).
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bioRxiv - Immunology 2021Quote: ... luciferase (Luciferase assay system, Promega) measurements were performed with 20 µl of lysed cells in white opaque plates (Greiner BioOne ...
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bioRxiv - Molecular Biology 2022Quote: ... and reverse transcription system (Promega). All reagent formulas are shown in S1 Table ...
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bioRxiv - Bioengineering 2022Quote: ... Renilla Luciferase Assay System (Promega) Quanti-Luc (Invivogen) ...
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bioRxiv - Bioengineering 2022Quote: ... GloMax-Multi+ Detection System (Promega). After 24 h ...
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bioRxiv - Molecular Biology 2023Quote: ... and GloMax Navigator System (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... and QuantiFluor dsDNA System (Promega). Final library pool was sequenced on Illumina NextSeq 500 with 75 bp single-ends ...
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bioRxiv - Cancer Biology 2023Quote: ... and QuantiFluor RNA System (Promega) respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... and QuantiFluor DNA System (Promega), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... and GloMax Navigator System (Promega).
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bioRxiv - Molecular Biology 2020Quote: ... Buffers were removed by two alternating washes of 50 mM TEAB and acetonitrile re-swelled on ice with 12.5 ng/μL trypsin (Promega, catalog # V5111) in 40 μL of 50 mM TEAB ...
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bioRxiv - Immunology 2022Quote: ... The reaction was shaken at room temperature for two minutes and the fluorescence values were read in a chemiluminescence detector (Promega GloMax). The neutralization NT50 titer was defined as the fold-dilution of serum necessary for 50% inhibition of luciferase activity in comparison with virus control samples.
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bioRxiv - Microbiology 2022Quote: ... washing two times with 100 µl PBS and then lysed with 1x lysis buffer (made from 5x Promega Firefly Lysis Buffer). Plates were frozen for at least 24 hours and then thawed for 2 hours before analyzing with Promega Firefly Luciferase Kit on a luminometer.
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bioRxiv - Cell Biology 2019Quote: ... were generated using PCR amplification of two DNA fragments and subsequent assembly PCR (using Pfu DNA polymerase from Promega, Mannheim, Germany) with the artificially inserted exon 9 sequence as region for annealing ...
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bioRxiv - Cell Biology 2023Quote: ... a 1839 bp region between two KpnI sites was amplified by PCR and subcloned into pGEM-T Easy (Promega, Madison, WI). This subclone was subjected to site-directed mutagenesis using the Q5 site-directed mutagenesis kit (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: Insertion sites in individual clones were mapped using a two-step nested arbitrary PCR approach using 2X GoTaq master mix (Promega M7122). The first reaction (1X GoTaq master mix ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were digested at 47 °C for two hours with 600 ng of mass spectrometry grade Trypsin/LysC mix (Promega, V5113). The digested peptides were then eluted with two 35 µl increments of 0.2% formic acid in water and two more 40uL increments of 80% acetonitrile with 0.2% formic acid in water ...
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... Amplicons for cloning were cut out from the gel and purified with the Wizard SV Gel and PCR Clean–up System kit (Promega, Madison, WI, USA). The other Archaea present in the last positive enrichment were identified following the cloning and sequencing of the 16S rRNA gene using the conventional archaeal universal primers A20F-1492R42,43 ...
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bioRxiv - Microbiology 2020Quote: ... of luciferase was determined using a PicaGene Luminescence Kit (TOYO B-Net Co., LTD, Tokyo, Japan) and GloMax Navigator System G2000 (Promega Corporation, Madison, WI), according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... and DNA-concentration was determined using a QuantiFluor® dsDNA kit (detection limit: 50 pg/mL; sensitivity: 0.01 – 200 ng/µL) and Glomax® –Multi+ system (Promega, Madison, WI) with the blue fluorescence optical kit installed (Ex ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were detached using AccutaseTM and RNA was extracted using the Promega kit ReliaPrep TM RNA Cell Miniprep System (Promega, US. Catalog# Z6011). RNA was reverse transcribed using Omniscipt TM RT kit (Qiagen ...
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bioRxiv - Genomics 2019Quote: ... amplicons from each replicate were pooled and purified with the Wizard SV Gel and PCR Cleanup System kit (Promega Corporation, Madison, Wisconsin, USA).
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bioRxiv - Cancer Biology 2021Quote: Genomic DNA was extracted from FFPE tumor tissue samples of 18 patients within the cohort using the automated Maxwell system with the Maxwell 16 FFPE Plus LEV DNA Purification Kit (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...