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Citations for Promega :
1 - 50 of 7454 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: Mammalian two-hybrid assay was performed using CheckMate™ Mammalian Two-Hybrid System (Promega, CAT# E2440) following the protocol supplied by the manufacturer ...
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bioRxiv - Biochemistry 2024Quote: Mammalian two-hybrid assays were performed following the instruction of the CheckMate™ Mammalian Two-Hybrid System (Promega). Constructs were synthesized by Genscript (Fig ...
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bioRxiv - Bioengineering 2019Quote: The two-hybrid system used in this study was developed from commercial CheckMate™ Mammalian Two-Hybrid System (Promega, Madison, WI, USA) with some modifications ...
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bioRxiv - Immunology 2022Quote: Based on Checkmate mammalian two-hybrid system (Promega, Madison, WI, USA), the indicated genes were engineered into pACT and pBIND vectors ...
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bioRxiv - Cancer Biology 2022Quote: Assays were performed using CheckMate Mammalian Two-Hybrid System (Promega E2440). The sequence encoding MED1 (amino acids 495-699) ...
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bioRxiv - Microbiology 2020Quote: The interactions between host protein and RHDV nonstructural proteins were evaluated using a CheckMate Mammalian Two-Hybrid System (Promega). The proteins expressed from the pACT vector recombinant plasmid acted as prey proteins ...
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bioRxiv - Cell Biology 2020Quote: ... and SERBP1 were amplified from human cDNA and cloned into the appropriate vectors (pACT or pBIND) provided by the CheckMate Mammalian Two-Hybrid kit (Promega E2440). The appropriate combination of edited pACT and pBIND vectors ...
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bioRxiv - Genetics 2020Quote: ... WT G4 mESCs (129/Sv × C57BL/6 F1 hybrid background)54 were co-transfected with the two respective sgRNA-pX459 vectors using the FUGENE HD transfection reagent (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Two days later the Dual-Glo Luciferase Assay System (E2940, Promega) was used to measure the luciferase activity of the cells on a bio-luminometer ...
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bioRxiv - Molecular Biology 2019Quote: ... Luciferase reporter gene construction The pGL3 Basic vector (Promega) was used as a backbone ...
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bioRxiv - Cancer Biology 2020Quote: ... RT-PCRs were conducted in two-step reactions by using two-step RT-PCR: M-MLV reverse transcriptase in M-MLV RT-PCR system (Promega, Madison, WI) and Platinum Taq polymerase (GeneMark ...
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bioRxiv - Evolutionary Biology 2021Quote: ... we extracted DNA from parents and hybrid fish using the Wizard SV Genomic DNA Purification kit (Promega), simultaneously digested samples with SphI and MluCI restriction enzymes (New England Biolabs) ...
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bioRxiv - Cancer Biology 2022Quote: ... the ratio of the two luciferases was determined (Dual luciferase kit, Promega). For generation of inducible BJAB cells ...
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bioRxiv - Molecular Biology 2022Quote: ... The firefly luciferase activity was detected in homogenates of organs by luminescence measurements using Synergy H4 hybrid reader (Bio-Tek) and Bright-Glo Luciferase Kit (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... Luminescence was measured using a two-injector dual system on the GLOMAX-Multi+ Microplate Multimode Reader (Promega). Relative luminescence units were calculated by normalizing oxyluciferin readouts to corresponding renilla measurements and in the HEK293T cells an additional normalization to non-transfected control cells was performed.
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bioRxiv - Microbiology 2019Quote: A Luciferase Assay System kit (Promega) was used ...
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bioRxiv - Plant Biology 2019Quote: The Reverse Transcription System Kit (Promega) was used to synthesize a cDNA library following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... or Luciferase Assay System kit (Promega) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... The Luciferase Assay System kit (Promega) was used to measure the luciferase activity in sporozoite samples following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Two micrograms of DNAse-free RNA was used for cDNA synthesis using an InProm-II Reverse transcription system (Promega) according to the manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2020Quote: ... Two micrograms of total RNA were used for cDNA synthesis with a reverse transcription kit (Promega). Quantitative real-time PCR was performed using the SYBR premix kit (Promega) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... total RNA was extracted separately from two 5 mg crushed-tissue samples by using the SV Total RNA Isolation System (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: Total RNA from two groups of samples were extracted using an SV total RNA isolation system with a DNase purification step (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Cells were harvested two days after transfection and firefly and Renilla luciferase activities were measured using the Dual-Luciferase reporter assay system (Promega). Total RNA was isolated using TriFast (Peqlab biotechnologies) ...
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bioRxiv - Microbiology 2022Quote: ... We estimated the number of infectious virus after two days by quantifying the luminescence in each well using Bright Glo Luciferase Assay System (Promega). Differences in relative luciferase units (RLUs ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: T7 RiboMAX Express RNAi System kit (Promega) was used to generate dsRNA-LvSTING dsRNA-LvcGAS and dsRNA-GFP (for controls ...
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bioRxiv - Biochemistry 2023Quote: Dual-Luciferase Reporter Assay System kit (Promega) was used according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... with the Reverse Transcription System kit (Promega) using random primers and adjusting the RNA volumes based on RNA concentrations.
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bioRxiv - Microbiology 2021Quote: ... gDNA was extracted from pooled DCR cultures from the two strain backgrounds using (Promega gDNA extraction kit) and stored at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Total genomic DNA of these two samples was obtained using Wizard Genomic DNA Purification Kit (Promega, Inc.) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the two luciferases were measured sequentially using the dual luciferase assay kit (Promega Corp, Madison, WI, USA) and a BMG FLUOstar OPTIMA microplate reader ...
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bioRxiv - Cancer Biology 2022Quote: ... After two rounds of trypsin (Promega) digestion ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Group two-choice assay: agarose (Promega) substrates were prepared as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated at 37 °C for two days and the nLuc activity from the virus replication was measured by using Nano-Glo® Luciferase Assay System (Promega) following the manufacturer’s protocol using a plate reader (HT4 Biotek) ...
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bioRxiv - Microbiology 2021Quote: ATPase activity of the purified proteins was determined using two kits – Enliten ATPase assay (FF2000, Promega, Moscow, Russia) and malachite green phosphate assay (MAK307 ...
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bioRxiv - Microbiology 2020Quote: ... Cell viability was measured two days post treatment with CellTiter-Glo Luminescent Cell Viability Assay kit (Promega, G7570) following standard protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Dual-Glo® Luciferase Assay System Kit (Promega) was used according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... A SV Total RNA Isolation System kit (Promega) was used to extract total mRNA ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... using the GoScript Revers Transcription System kit (Promega). RT was performed in an Eppendorf Mastercycler to synthetize cDNAs ...
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bioRxiv - Neuroscience 2022Quote: ... then lysed with 20μL 1x passive lysis buffer from Promega dual-luciferase assay system kit (Promega ref.# E1960) and placed on the shaker for 15-20 minutes ...
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bioRxiv - Cancer Biology 2023Quote: The SV total RNA isolation system kit (Promega) was employed to isolate RNA from cells ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were harvested two days post-transfection and lysed using the Passive Lysis Buffer provided in the Dual-Luciferase Reporter Assay System (Promega, Madison, WI). The relative activities of the firefly luciferase and renilla luciferase domains in the clarified lysate were then measured using the Dual-Luciferase Reporter Assay System on a Synergy Neo2 plate reader (Biotek ...
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bioRxiv - Cancer Biology 2021Quote: FGFR1 kinase enzyme system kit was purchased from Promega Inc ...
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bioRxiv - Cell Biology 2020Quote: A GenePrint 10 System PCR Amplification kit (Promega Corporation) was used to determine the genetic signature of both samples based on the multiplex analysis of 9 loci and the Amelogenin sex-determining marker ...
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bioRxiv - Microbiology 2019Quote: ... coli S30 Extract system for Linear Templates kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Dual luciferase assay system kit (Promega, Madison, WI, USA) was used following manufacturer’s protocol as described previously51.
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bioRxiv - Molecular Biology 2023Quote: ... and Dual-GLO®Luciferase Assay System Kit (Promega). Firefly luciferase activities were normalized to Renilla luminescence in each well.
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bioRxiv - Developmental Biology 2023Quote: ... using the T7/T3 Riboprobe Combination System kit (Promega) and the Digoxigenin labeling kit (Roche) ...
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bioRxiv - Microbiology 2024Quote: ... Wizard Plus SV Minipreps DNA Purification System kit (Promega) was used following the Centrifugation Protocol under Quick Protocol ...
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bioRxiv - Microbiology 2024Quote: ... The Steady-Glo Luciferase Assay System kit from Promega was used for all luciferase assays ...