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Citations for Promega :
301 - 350 of 1197 citations for Transcription Factor 15 TCF15 Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... as previously described75 before in vitro transcription using the T7 RiboMAX system (Promega) following the manufacturer protocol but with all volumes and concentrations reduced by a factor of two ...
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bioRxiv - Cell Biology 2023Quote: ... and cDNA synthesis using the GoScript Reverse Transcription Mix Random Primers (A2800, Promega) were done according to the instructions of the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: ... DNase digested and reverse-transcribed using the Improm-II Reverse Transcription System (Promega). qPCR was performed from 100 ng of gDNA using the Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: Translation reactions were performed using the TNT Quick Coupled Transcription/Translation kit (Promega). 10-50 ml reactions were assembled according to kit directions and labelled using EasyTag L-[35S]-Methionine (Perkin-Elmer) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Purified RNA was reverse-transcribed into cDNAs with GoScript Reverse Transcription System (Promega) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2023Quote: ... In vitro protein synthesis by the coupled transcription and translation (TnT) system (Promega) was carried out in the presence of 35S methionine in either wheat germ or rabbit reticulocyte lysates ...
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was performed with M-MLV RT Enzyme (Promega, Madison, WI, USA) and oligo-dT (T12 and T16 mix ...
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bioRxiv - Biochemistry 2023Quote: ... Reverse transcription to cDNA was conducted using M-MLV Reverse Transcriptase (Promega, M1701) following the manufacturer’s instructions with subsequent confirmation of transcription using cyclophilin qPCR.
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bioRxiv - Immunology 2023Quote: ... cDNA was prepared by reverse transcription (GoTaq 2-Step RT-qPCR System, Promega) and amplified byPCR using SYBR® Green PCR Master Mix and ABI Prism7900HT sequence detection system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2023Quote: ... and cDNA was prepared as per the GoScript™ Reverse Transcription System (Promega) protocol ...
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bioRxiv - Immunology 2023Quote: ... cDNA was prepared by reverse transcription (GoTaq 2-Step RT-qPCR System, Promega) and amplified by PCR using SYBR® Green PCR Master Mix and ABI Prism 7900HT sequence detection system (Applied Biosystems) ...
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bioRxiv - Genetics 2024Quote: ... cDNA was synthesized using the ImProm-II™ Reverse Transcription System (Promega, USA). The mRNA composition was established by PCR with plasmid-specific primers ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were also co-transfected with 15 ng pTK-Renilla (Promega) plasmids using Lipofectamine 3000 reagent (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2020Quote: ... or 15 mL of 1-to-50 diluted NanoLuc substrate (Promega), was pipetted into a 384-well white flat bottom plate (Greiner) ...
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bioRxiv - Plant Biology 2020Quote: ... using M-MLV reverse transcriptase and oligo(dT)15 primer (Promega). Two ng of cDNA was used as template in qPCRs ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNAs were amplified by 15 PCR using GoTaq G2 polymerase (Promega) and TruSeq_adaptor_fwd and TruSeq_adaptor_rev primers (Table S1) ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were lysed for 15 min in passive lysis buffer (Promega) with gentle shaking and homogenized lysate was transferred to the wells of an opaque white 96-well plate (Corning) ...
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bioRxiv - Biochemistry 2021Quote: ... A 15-amino acid linker and SmBiT subunit (peptide 86, Promega) were attached to the C-terminus of rat Gβ1 ...
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bioRxiv - Physiology 2021Quote: ... with D-luciferin potassium salt (15 mg/kg, Promega, Madison, WI) in sterile phosphate-buffered saline ...
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bioRxiv - Genomics 2022Quote: ... cells were co-transfected with 15 fmol pRL-TK (Promega, E2241) and 150 fmol of either pGL4.27[luc2P/minP/Hygro] empty vector or pGL4.27[luc2P/minP/Hygro] containing either eSOX17 ...
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bioRxiv - Biochemistry 2023Quote: ... 15 μl reactions contained 60% nuclease-treated rabbit reticulocyte lysate (Promega), 20 μM amino acid mixture minus leucine ...
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bioRxiv - Plant Biology 2024Quote: ... according to the instructions: stock oligo(dT)15 primer (C1101, Promega) concentration was 100 ng/μl ...
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bioRxiv - Immunology 2021Quote: ... RNA was subjected to reverse transcription using the GoScript™ Reverse Transcriptase system (Promega). All qPCRs were performed in MicroAmp Fast Optical 96 WellReaction Plates (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: Total RNA was reverse transcribed into cDNA using the GoScript Reverse Transcription System (Promega) and quantified by qPCR using SYBR GREEN mix (Vazyme Q111–02 ...
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bioRxiv - Neuroscience 2021Quote: ... Myc-tagged MiD49 and MiD51 were produced via in vitro transcription/translation (IVT) (Promega). For in vitro ubiquitination assays ...
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bioRxiv - Cell Biology 2019Quote: Reverse transcription was carried out with 2µg of total RNA and random primers (Promega), followed by treatment with RNase H (New England Biolabs) ...
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bioRxiv - Biochemistry 2019Quote: ... The plasmid pGEM-1 and the TNT SP6 Transcription/Translation System were from Promega. [35S]Met was from PerkinElmer.
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bioRxiv - Neuroscience 2020Quote: ... RNA was reverse transcribed into cDNA using a ImProm-II Reverse Transcription System (Promega). cDNA was mixed with Luna Universal qPCR Master Mix (NEB) ...
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bioRxiv - Plant Biology 2021Quote: Reactions were performed using the TNT® T7 Quick Coupled Transcription/Translation System (Promega) with 1 μg of vector (NPH3 or variants in pGADT7 ...
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bioRxiv - Cell Biology 2021Quote: Proteins were synthesized using TNT® SP6 Quick Coupled Transcription/Translation system (Promega L2080) using the standard reaction mix (rabbit reticulocyte lysate plus amino acids ...
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bioRxiv - Synthetic Biology 2021Quote: Transcription-based luciferase reporter assays were performed with the Dual-Glo kit (Promega; E2920). HEK cells were transfected in a 96-well plate with GPCR or empty vector (95ng) ...
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bioRxiv - Plant Biology 2021Quote: ... cDNAs were prepared by GoScript™ Reverse Transcription System following manufactuere’s protocol (Promega, USA). All qRT-PCRs were performed in ABI 7300 real-time PCR machine (Applied Biosystem ...
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bioRxiv - Microbiology 2020Quote: ... then labeled with 35S-Methionine (Amersham Pharmacia Biotech) in vitro transcription-translation system (Promega), respectively ...
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bioRxiv - Plant Biology 2020Quote: ... and reverse transcribed with random primers using the GoScript Reverse Transcription kit (Promega, USA). Reverse transcription quantitative PCR (RT-qPCR ...
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bioRxiv - Neuroscience 2022Quote: ... Total RNA was reverse transcribed at 42°C by using GoScript Transcription System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... Reverse transcription was performed with random hexamer primers using an MMLV-RT platform (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... and cDNA was generated using GoScript Reverse Transcription System following the manufacturers protocol (Promega). Viral RNA was detected using the CDC 2019-Novel Coronavirus Real-Time RT-PCR Diagnostic Panel as per Centre for Disease and Control’s optimised protocol (Integrated DNA Technologies ...
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bioRxiv - Molecular Biology 2020Quote: Strand-specific RNA probes were generated using a T3 in vitro transcription kit (Promega) in the presence of Chromatide AlexaFluor-UTP (ThermoFisher) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... In vitro reverse transcription was performed using the RiboMax T7 system (Promega, Madison, WI), and the product was biotinylated using the Pierce RNA 3’ End Desthiobiotinylation Kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... cDNA was prepared using ‘ImProm-II™ Reverse Transcription System’ (Promega cat No: A3800) according to the kit protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... Reverse transcription-PCR was performed using a cDNA synthesis kit (Promega, Madison, WI, USA) and 2 μg of total RNA according to the manufacturer's protocol ...
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bioRxiv - Microbiology 2019Quote: ... The RNA was reverse transcribed into cDNA using the GoScriptTM Reverse Transcription System (Promega). Quantitative real-time polymerase chain reaction (PCR ...
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bioRxiv - Cancer Biology 2019Quote: ... Complementary DNA (cDNA) was prepared using ImProm-II™ reverse transcription kit (Promega, A3800) and 10 ng of cDNA were used as template for each real-time PCR reaction ...
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bioRxiv - Developmental Biology 2020Quote: ... [35S]-labeled Zc3h10 protein was produced by using TNT coupled transcription/translation kit (Promega). 20 ug of GST fusion proteins were incubated for 2 hrs at 4°C with in vitro translated Zc3h10 and glutathione sepharose beads ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA synthesis was carried out using the GoScript Reverse Transcription system (Promega, USA) using OligoDT ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was generated from the RNA using a GoScript™ Reverse Transcription System (Promega) according to the vendor’s protocol ...
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Ion transport modulators differentially modulate inflammatory responses in THP-1 derived macrophagesbioRxiv - Immunology 2021Quote: ... after which cDNA was synthesised using the GoScript reverse transcription system (Promega, Madison, USA). Quantitative PCR was performed using the Mesa green qPCR master mix (Eurogentec ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA synthesis was performed by the ImProm-II™ Reverse Transcription System (Promega) and oligo(dT) ...
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bioRxiv - Microbiology 2021Quote: ... Afterwards RNA was converted into cDNA using a GoScript™ Reverse Transcription kit (Promega), and stored at −20°C until analysis ...
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bioRxiv - Physiology 2021Quote: ... RNA was reverse transcribed into cDNA using the ImProm-IITM Reverse Transcription System (Promega) following manufacturer’s recommendations ...