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Citations for Promega :
501 - 550 of 1197 citations for Transcription Factor 15 TCF15 Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Reverse transcription of the extracted RNA was performed using 1μl of 0.5μg/μl OLIGO dT primers (Promega) and 1μl of RNase free-water and incubating samples at 70°C for 10 minutes and cooling for 5 minutes ...
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bioRxiv - Microbiology 2021Quote: ... ribosome positioning was determined by primer extension/reverse transcription using AMV reverse transcriptase (1 U/μl) (Promega) in the presence of 125 μM of each of dTTP ...
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bioRxiv - Molecular Biology 2020Quote: In vitro transcription/translation assays were carried out in a S30 T7 HighYield Protein Expression System (Promega). The full lenght Hrnpk cDNA template was cloned from total RNA obtained from GH4C1 cells and transferred to a pET28a Escherichia coli T7 expression vector (Novagen ...
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bioRxiv - Molecular Biology 2019Quote: ... Reverse transcription was performed with random hexamer primers (R6) and M-MLV reverse transcriptase (Promega, Madison, WI) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... the reaction mixtures for in vitro transcription were treated with RQ-1 RNase-free DNase I (Promega). Synthesized mRNAs were treated with phenol-chloroform to remove protein components ...
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bioRxiv - Microbiology 2020Quote: ... RNA (1 μg) was converted to cDNA using the GoScript™ Reverse Transcription System (Promega, Madison, WI). The cDNA was synthesised was used for qPCR using the iQTM SYBR® Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 800 ng total RNA using GoScriptTM Reverse Transcription Mix using oligo(dT) (Promega). Real-time quantitative PCR was performed by the Taq-Man (rice ...
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bioRxiv - Plant Biology 2020Quote: ... Samples of RNA were reverse transcribed using the GoScript™ reverse transcription system and oligo (dT20) (Promega). RT–qPCR was performed in a 20 μl reaction with the Fast Start Universe SYBR Green Master (ROX ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were in vitro translated using TnT Quick Coupled Transcription/Translation System (Promega, USA, Cat. No. L1170) according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2019Quote: ... cDNA was generated from 500 μg purified RNA with the ImProm-II™ Reverse Transcription System (Promega) primed by random primers ...
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bioRxiv - Developmental Biology 2020Quote: ... and cDNA was synthesized by reverse transcription using the First-Strand cDNA Synthesis Kit (Promega, Madison, WI). qPCR reactions were performed using Bio-Rad SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Biochemistry 2019Quote: In vitro transcription reactions were carried out using T7 RiboMAX™ Large Scale RNA Production System (Promega) in 10 µl volumes ...
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bioRxiv - Pathology 2021Quote: ... 300 ng of RNA was used to synthesize cDNA using the GoScript™ Reverse Transcription System (Promega). Gene specific primers for Actin (Pn_Actin_qPCR_F and Pn_Actin_qPCR_R) ...
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bioRxiv - Molecular Biology 2020Quote: ... A 5µl aliquot of the sample was added to a 10µl ImProm-II reverse transcription reaction (Promega). The ssDNA product was used as a template for 25 cycles of PCR using a 50µl GoTaq reaction (Promega).
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bioRxiv - Evolutionary Biology 2020Quote: ... and 700 ng used for in vitro transcription/translation using TnT T7 Quick for PCR DNA (Promega) and the Fluorotect GreenLys tRNA (Promega ...
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bioRxiv - Cell Biology 2020Quote: KNL-2-MBP fragments were [35]S labeled using TnT® Quick Coupled Transcription/Translation System (Promega). 10 μl of the in vitro translation lysate was incubated for 1hr at 4°C with 50 μg CENP-AHCP-3(1-109)-MBP-His (in 20 mM Tris [pH7.5] ...
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bioRxiv - Developmental Biology 2020Quote: ... 6 uL of RNA was used for each cDNA synthesis (GoScript™ Reverse Transcription System kit, Promega). qPCR was performed using Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Physiology 2021Quote: ... 500 ng of total RNA were reverse transcribed using the GoScript Reverse transcription system kit (Promega, USA), following manufacturer instructions and cDNA samples were then stored at - 20°C until further use.
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bioRxiv - Cell Biology 2021Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (SP6 or T7, Promega). Translation products were partially purified by ammonium sulfate precipitation (Bardwell & Shah ...
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bioRxiv - Neuroscience 2021Quote: ... ImProm-II™ Reverse Transcription System and GoTaq® DNA polymerase were purchased from Promega (Madison, WI). The RNA Clean & Concentrator™-5 was purchased from Zymo Research (Irvine ...
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Psi promotes Drosophila wing growth through transcriptional repression of key developmental networksbioRxiv - Developmental Biology 2020Quote: ... 5 µL of RNA was used for each cDNA synthesis (GoScript™ Reverse Transcription System kit, Promega). qPCR was performed using Fast SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesised from 2 μg RNA using the GoScriptTM Reverse Transcription kit (Promega, UK, Cat. A5003). Primers were designed using NCBI BLAST primer design or ...
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bioRxiv - Evolutionary Biology 2021Quote: Reverse transcription was performed in 20μL final reaction volumes with the GoScript kit (Promega, Madison, WI, USA) and random hexamers (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... Eukaryotic in vitro Nluc expression was performed with TnT-T7 Quick Coupled Transcription/Translation System (# L1170, Promega) with 100 ng of pT7CFE1-NLuc plasmid DNA ...
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bioRxiv - Immunology 2021Quote: ... and used to generate 35S-methionine labelled AIM2 protein by in vitro transcription and translation (IVTT) (Promega). Immunoprecipitations (IP ...
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bioRxiv - Genomics 2022Quote: ... Reverse transcription was carried out using moloney-murine leukemia virus (M-MLV) reverse transcriptase kit (Promega, USA). Reverse transcription was completed in a 30 µL final volume reaction containing 400 U M-MLV and 1 mM dNTP mix (Invitrogen ...
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bioRxiv - Genetics 2022Quote: Total RNAs were reversed into first-strand cDNA using the M-MLVRT reverse transcription kit (Promega, USA). qRT-PCR was performed with SYBR Premix Ex Taq (TaKaRa Biotech Co. ...
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bioRxiv - Microbiology 2022Quote: In vitro sgRNA transcription was performed using the Ribomax T7 large-scale RNA production kit (Promega, #P1300) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... [35S]-methionine-labelled proteins were transcribed and translated using a TnT™ coupled transcription–translation kit (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... the extracted RNA was reverse transcribed by using the reverse transcription system (Promega Corporation, Fitchburg, WI, USA). For this ...
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bioRxiv - Microbiology 2024Quote: ... the extracted RNA was reverse transcribed by using the reverse transcription system (Promega Corporation, Fitchburg, WI, USA). For this ...
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bioRxiv - Biochemistry 2020Quote: ... Media were removed and cells were lysed for 15 min in passive lysis buffer (Promega) with gentle shaking and homogenized lysate was transferred to the wells of an opaque white 96-well plate (Corning) ...
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bioRxiv - Plant Biology 2022Quote: ... according to the instructions with the following modifications: stock oligo(dT)15 primers (C1101, Promega) concentration was 50 ng/μl and 0.5 μl Superscript II enzyme was used per reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... Each fraction was digested for 15–18h at 37°C using ultra-grade Trypsin (Promega) at a 1:100 enzyme ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was primed with oligo(dT)15 using M-MLV reverse transcriptase (Promega, https://france.promega.com). Relative transcript levels were determined with the LightCycler 480 SYBR Green I Master kit (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... purified Halo-tagged MultiDsk (15 μg per condition) was pre-incubated with HaloLink resin (Promega) for 1 h with rotation at room temperature in binding buffer (100 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... plates were placed for 15 min at RT to equilibrate before NanoBRET NanoGlo Substrate (Promega) was added ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% Triton X-100) with the addition 15 ng of Lambda DNA (Promega, Fitchburg, WI) in 20 µL total reaction volume for 3 h at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... knowlesi elongation factor-1 alpha (pkef1-alpha) 5’ UTR were isolated from agarose gels and cloned into the pGEM-T vector (Promega, Madison, WI, USA). To replace the pfcam 5’ UTR sequence that drives luciferase transcription in the pD-pfcam-Luc ...
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription was performed on 1 μg of RNA with random hexamer primers using MMLV-RT enzyme (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... and 1μg RNA was reverse transcribed to cDNA using an AMV reverse transcription system (Promega, Madison, WI, USA). Primers ...
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bioRxiv - Cell Biology 2020Quote: ... and biotinylated in a single reaction with the TnT® SP6 Quick Coupled Transcription/Translation System (Promega, L2080) and Transcend™ tRNA (Promega L5016) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μg of DNA-free RNA was then used for reverse transcription using M-MLV Reverse Transcriptase (Promega) in the presence of RiboLock ...
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bioRxiv - Pathology 2022Quote: ... A total of 1 µg of extracted RNA was transcribed into cDNA using GoScript Reverse Transcription Kit (Promega). Quantitative real-time PCR was performed using FastStart SYBR Green Master mix (Roche ...
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bioRxiv - Molecular Biology 2021Quote: RNAs transcripts were generated by in vitro transcription using the RiboMAX™ Large Scale RNA Production Systems (Promega). Reactions were set up by adding 2 µL of the T7 Transcription 5X Buffer ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription (RT) of different HCV genomic regions was performed using avian myeloblastosis virus (AMV) reverse transcriptase (Promega), and subsequent PCR amplification was carried out using AccuScript (Agilent Technologies) ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μg of DNAse free RNA was used for cDNA synthesis using ImProm-II Reverse transcription system (Promega), as per the manufacture’s recommendation ...
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bioRxiv - Plant Biology 2019Quote: ... The synthesis of the first strand of cDNA was performed by an ImProm-II Reverse Transcription System (Promega) using Oligo(dT)15 primers according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2020Quote: The in vitro translation analysis was performed using the TnT® Quick Coupled Transcription/Translation System from Promega by following the manufacture’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: RNAs transcripts were generated by in vitro transcription using the RiboMAX™ Large Scale RNA Production Systems (Promega). Annealed oligonucleotides forward and reverse containing the T7 promoter and the sRNA were used as template ...