Labshake search
Citations for Promega :
551 - 600 of 1197 citations for Transcription Factor 15 TCF15 Antibody FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... served as template for cDNA synthesis by random priming using M-MLV reverse transcription system (Promega, Madison, WI). qPCR was performed based on SYBRgreen I technology using the ORA qPCR Green ROX L Mix (HighQu ...
-
bioRxiv - Molecular Biology 2020Quote: In vitro transcription/translation of vvl/dfr vDNA was carried out using TNT coupled reticulo lysate system (Promega) and T3 RNA polymerase (New England Biolabs Inc. ...
-
bioRxiv - Developmental Biology 2022Quote: ... cDNA was synthesized from 100-500 ng of total RNA using the GoScript reverse transcription reaction kits (Promega). Singleplex quantitative real-time polymerase chain reaction (qPCR ...
-
bioRxiv - Molecular Biology 2019Quote: ... was added to 500 ng of the cDNA-fragments in T7 transcription reaction buffer (Promega, Cat no. P2075), and incubated for 2 hours at 37°C in the absence or presence of N6-methyladenosine (Jena Biosciences ...
-
bioRxiv - Molecular Biology 2020Quote: ... RNA was synthesized by in vitro transcription of the PCR products using the T7 RNA polymerase (Promega kit). Then ...
-
bioRxiv - Cell Biology 2019Quote: ... In vitro translated (IVT-) proteins were synthesized using a TNT quick-coupled transcription-translation kit (Promega, Madison, WI). For synthesizing the Pav and PavDEAD proteins ...
-
bioRxiv - Immunology 2019Quote: ... Reverse transcription of 3.5 μg isolated RNA was achieved with M-MLV reverse transcriptase (M1705, Promega, WI, USA) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... were transcribed and translated using the TNT T7 Quick for PCR DNA Coupled Transcription/Translation System (Promega, USA). The reactions contained 10 µL of TNT ...
-
bioRxiv - Microbiology 2021Quote: In vitro transcription and translation reactions were conducted using the TNT coupled rabbit reticulocyte lysate system (Promega, L4610) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... 1 μg of DNA-free RNA was then used for reverse transcription using M-MLV Reverse Transcriptase (Promega) in the presence of RiboLock ...
-
bioRxiv - Microbiology 2022Quote: ... The reverse transcription (RT) mix contained 200 units of recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase (Promega), 20 units of RNAsin (Promega) ...
-
bioRxiv - Microbiology 2022Quote: ... reverse transcription was performed with 0.5 μl (corresponding to 5 U) of Avian Myeloblastosis Virus Reverse Transcriptase (Promega), 0.1 μl dNTP mix (10 mM) ...
-
bioRxiv - Cell Biology 2023Quote: 35S-radiolabeled Tom40 precursor proteins were synthesized in vitro using the TNT Quick Coupled Transcription/Translation kit (Promega). Transcripts for MCO6 encoding 3 additional N-terminal methionine residues for radiolabelling of the Mco6 protein which lacks internal methionine residues ...
-
bioRxiv - Molecular Biology 2023Quote: A total of 10 µg RNA was taken from each sample for reverse transcription (RT) using GoScript (Promega) reverse transcriptase (5 µg for RT ...
-
bioRxiv - Molecular Biology 2023Quote: ... The digested vector was subjected to invitro transcription (IVT) following the manufacturer’s protocol using T7 polymerase (Promega P2075) and biotin RNA labeling mix (Roche 11685597910).
-
bioRxiv - Microbiology 2023Quote: ... reverse transcription reactions were performed by adding 0.5 µl (corresponding to 5 U) of AMV Reverse Transcriptase (Promega), 0.1 µl dNTP mix (10 mM) ...
-
bioRxiv - Microbiology 2023Quote: ... AePer50 reverse transcription (RT) and qPCR reactions were performed using GoTaq® 1-Step RT-qPCR System (Promega), with 10 µl of 2X qPCR Master Mix ...
-
bioRxiv - Developmental Biology 2023Quote: ... RT-PCR for mRNA transcripts was performed by using the ImProm-II Reverse Transcription System kit (Promega A3800). Real Time PCR was performed with Applied Biosystems Fast SYBR Green Master Mix (Thermo Fisher Scientific ...
-
bioRxiv - Physiology 2023Quote: ... cDNA was prepared according to the manufacturer’s protocol using the ImProm-II reverse transcription system (Promega, Madison, WI). Quantitation of gene expression was performed by real-time RT-PCR Quant Studio 3 (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... and was reverse transcribed to first strand cDNA using the Reverse Transcription System (A3500, Promega, Madison, Wisconsin, USA). RT–PCR with the forward primer (5’-TGCTTCGAATGCACTCATCACAAC-3′ ...
-
bioRxiv - Molecular Biology 2024Quote: In vitro translation (IVT) of each eIF4E was performed using the TNT Quick Coupled Transcription/Translation System (Promega) according to the manufacturer’s protocol and based on a protocol developed to study zebrafish eIF4E (111) ...
-
bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized from 1 μg of each RNA sample using Oligo(dT)15 Primer (Promega) and M-MLV Reverse Transcriptase (RNase H Minus ...
-
bioRxiv - Microbiology 2023Quote: ... 15 µl samples were allowed to cool to room temperature and 1 µl RQ1 DNase (Promega) diluted to 0.1 U/µl in storage buffer (10 mM Hepes pH 7.5 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... and incubated for 15’ with Proteinase K (1:500, in 1XPBS, Promega B.V., Leiden, The Netherlands) at 37°C ...
-
bioRxiv - Neuroscience 2024Quote: ... Protein samples (15 µg) were digested overnight at 37 °C with trypsin (Promega, Madison, WI-USA) in a protein:trypsin ratio of 50:1 (w/w) ...
-
bioRxiv - Biochemistry 2024Quote: ... plates were equilibrated to RT for 15 min and CellTiter Glo reagent (Promega, Madison, WI, USA) was added followed by orbital shaking for 2 min ...
-
bioRxiv - Microbiology 2024Quote: ... cultures were pelleted (1,800xg for 15 min) and resuspended in 100 μl NanoLuc Assay solution (Promega). The homogenized cell suspensions were transferred into white flat-bottom 96-well plates and incubated for 1 h at RT while protected from light ...
-
bioRxiv - Cancer Biology 2021Quote: ... T7 expression vectors were used in an in vitro translation reaction using TNT Quick Coupled Transcription/Translation System (Promega), according to manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2021Quote: ... and mouse cyclin B1 were generated using the Riboprobe in vitro Transcription Systems kit according to the manufacturer’s protocol (Promega). Anti-BrdU antibodies were conjugated with protein A-sepharose beads overnight at 4C (GE Healthcare) ...
-
bioRxiv - Neuroscience 2020Quote: ... digoxigenin (DIG)-labeled riboprobes were generated with a commercial transcription kit (Riboprobe System, P1440, Promega, San Luis Obispo, CA) and DIG RNA labeling mix (Roche REF# 11277073910 ...
-
bioRxiv - Cell Biology 2019Quote: ... 500 ng RNA was used for reverse transcription using random primers and the GoScript master mix according to the manufacturer’s instructions (Promega). Quantitative real-time PCR was used to examine the expression levels of TRAP1 and HIF1A with GAPDH as the reference gene.
-
bioRxiv - Molecular Biology 2019Quote: ... and C-terminally truncated VP16 (VP16ΔC) proteins were synthesized using a rabbit TNT quick-coupled transcription/ translation system (Promega) with 35S-labeled methionine ...
-
bioRxiv - Plant Biology 2020Quote: ... DNA-free RNA (500 µg) was used for reverse transcription using the Moloney Murine Leukemia Virus Reverse transcriptase (Promega, Madison ...
-
bioRxiv - Immunology 2021Quote: Total RNA was extracted from sorted cells with RiboEx (GeneAll) and 0.2–1 μg was subjected to cDNA synthesis using the GoScript Reverse Transcription system (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2021Quote: mRNA pools were prepared by in vitro transcription using the RiboMAX Large Scale RNA Production System-T7 (Promega, USA), and prepared DNA templates ...
-
bioRxiv - Plant Biology 2021Quote: ... 10 ng of RNA was used with oligo (dT) primers and AMV reverse transcriptase and reverse transcription was performed according to the manufacturer’s instructions (Promega). Using a GeneAmp 7500 Sequence Detection System (Applied Biosystems) ...
-
bioRxiv - Cell Biology 2021Quote: ... cDNA synthesis was performed via reverse-transcription of 2µg of RNA using M-MLV Reverse Transcriptase (Promega (Wisconsin, USA). cDNA was used to analyze silencing efficiency by qRT-PCR employing iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories ...
-
bioRxiv - Microbiology 2022Quote: ... for 1 h at 37 °C and cDNA was then synthesized using a Moloney murine leukemia virus (MMLV) reverse transcription kit with random primers following the manufactureŕs protocol (Promega). To check for the presence of remaining DNA ...
-
bioRxiv - Developmental Biology 2020Quote: ... The cDNA was synthesized from 1 μg of the total RNA using the ImProm-II Reverse Transcription System (Promega) and oligo (dT)23 primers.
-
bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized from 400 ng of RNA using ImProm-II Reverse Transcription system kit and random primers (Promega). Quantitative PCR was performed in technical duplicates using SensiMix SYBR mix (Bioline) ...
-
bioRxiv - Pathology 2022Quote: ... G490 5 × All-In-One RT MasterMix (abm) was used for reverse transcription according to the manufacturer’s protocol (Promega). The qRT-PCR analysis was performed in 96-well plates using the BIO-RAD CFX96 detection system ...
-
bioRxiv - Plant Biology 2019Quote: ... In situ probes were made using T7/SP6 promoter based in vitro transcription in the cloning vector pGEMT (Promega). FAA (Formaldehyde Acetic Acid ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... Synthesis of cDNA was performed with 1 μg of total RNA by using the GoScriptTM Reverse Transcription System (Promega), according to the manufacturer’s recommendations ...
-
bioRxiv - Cancer Biology 2019Quote: ... A total of 500 ng RNA was reverse-transcribed to cDNA using the Reverse Transcription Kit (Promega, Madison, USA), according to the manufacturer’s instruction ...
-
bioRxiv - Developmental Biology 2019Quote: ... and 1 μg of RNA was reverse-transcribed by the oligo (dT) primer using a reverse transcription kit (Promega) after digestion with RNase-free DNaseI (Fermentas) ...
-
bioRxiv - Cell Biology 2020Quote: ... Reverse transcriptase reaction was performed from 2 µg of total RNA by using ImProm-IITM Reverse Transcription System (Promega). Aliquots of cDNA obtained were amplified in PCR and quantitative real-time PCR reactions ...
-
bioRxiv - Pathology 2020Quote: ... One thousand ng of RNA was reverse transcribed to cDNA using reverse transcription reagents (Go Script, Promega, Madison, WI) per manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2020Quote: ... Five hundred ng total RNA was reverse transcribed using oligo(dT) and the MLV reverse transcription system following the manufacturer’s instructions (Promega). qRT-PCR was performed in 25 μL reaction volumes with gene-specific primers and conducted with a StepOne Plus Real-time PCR System (Applied Biosystems ...
-
bioRxiv - Plant Biology 2020Quote: ... template cDNA samples were prepared using 4 κg of total RNA and the Improm-II reverse transcription system (Promega). Primers were designed to have a Tm of ~60°C and to produce PCR products of ~200−400 base pairs ...
-
bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized by reverse transcription using RevertedAid H minus reverse transcriptase (ThermoFischer Scientific) and random hexamer primers (Promega), in combination with a primer targeting the 3’ end of the MERS-CoV genome ...