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Citations for Promega :
701 - 750 of 1243 citations for Rabbit Anti Sheep IgG H+L FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... 70 μL of PBS were added to each well followed by addition of 10 μL of a 50 μM coelenterazine-h solution (Promega). After 10-min incubation ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were cultured for 48 h and the luciferase activity was measured using the Dual-Luciferase Reporter Assay System (Promega). The final firefly luciferase activity was normalized to the co-expressed renilla luciferase activity.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Coelenterazine h was purchased from Nanolight Technologies (Pinetop, AZ, USA) and furimazine (NanoGlo) was purchased from Promega (Madison, WI, USA).
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bioRxiv - Biochemistry 2022Quote: ... Samples were diluted with ddH2O 1:5 and proteins were digested for 20 h at 37°C using trypsin (Trypsin Gold, Promega) at an enzyme/protein ratio of 1:50 ...
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bioRxiv - Immunology 2022Quote: ... for 48 h after which cells were washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Microbiology 2022Quote: ... Luciferase activity (Relative Luciferase Units or RLU) was detected at 72 h post-infection by using Bright-Glo Luciferase Assay System Kit (Promega) in a Microplate Luminometer (Wallac-Perkin Elmer).
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bioRxiv - Cancer Biology 2022Quote: ... Treatments were removed 48 or 72 h later and enzymatic activities of caspase-3 and -7 were measured using Caspase-Glo 3/7 Assay (Promega) and read by a microplate reader (SpectraMax M5).
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bioRxiv - Evolutionary Biology 2022Quote: ... the proteins were alkylated with iodoacetamide (100 mM for 1 h at room temperature in a dark chamber) and digested with trypsin (Promega) diluted in ammonium bicarbonate (0.1 M for 24 h at 37 °C) ...
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bioRxiv - Cancer Biology 2022Quote: ... Both luciferase activities were measured 48 h later after the transient transfection with different plasmid combinations in accordance with the manufacture’s protocols (Promega, USA). All results were normalized to the Renilla Luciferase activity.
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bioRxiv - Microbiology 2021Quote: ... Luciferase activity was measured 16 h post-infection using the Bright-Glo Luciferase Assay System or ONE-Glo Luciferase Assay System (Promega) and Centro xS960 luminometer (Berthold).
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bioRxiv - Cell Biology 2021Quote: ... following the manufacturer’s protocols and 250-500 ng of RNA was used to generate a cDNA library with MMLV H-Reverse transcriptase (Promega) and Oligo dT15 primers (Promega ...
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bioRxiv - Molecular Biology 2019Quote: Cell viability was determined 72 h after PDS or cisplatin treatment using a CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... purified total RNA was synthesized into cDNA:RNA hybrids with Maxima H Minus Reverse Transcriptase (Thermo) and primed using equal amounts of oligo(dT)15 primers (Promega) and random hexamers (Thermo) ...
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bioRxiv - Cancer Biology 2021Quote: ... 293T cells were transfected with 8xTEAD synthetic YAP/TAZ-responsive promoter-luciferase reporter and treated with BSA or recombinant TNC (5 µg/mL) for 48 h to measure YAP1 signaling activity using Dual-luciferase Reporter Assay Kit (Promega).
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bioRxiv - Microbiology 2022Quote: 293T cells were co-transfected with pEGFP-N1 and plasmids expressing SARS-CoV-2 Spike (FL) or SARS-CoV-2 Spike-Δ19 for 24 h using FugeneHD (Promega). The cells were treated with DMSO or 10 µM 6-TG at 4 h post- transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μL of the annealed oligos were ligated with 0.04 ng digested PspCAs13b crRNA backbone in 10 μL of T4 ligation buffer (3 h, RT) (Promega, M1801). All PspCas13b crRNA spacer sequences used in this study are listed in Supplementary File 1 ...
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bioRxiv - Microbiology 2020Quote: ... Protein digestion was performed using 1:100 (w/w) LysC (Wako Chemicals) for 4 h at 37°C and then finalised with 1:50 (w/w) trypsin (Promega) overnight at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were harvested 36 h after transfection by lysing in 100 μl of 1× reporter lysis buffer (Promega, Madison, WI). Lysates were pelleted ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were harvested 24 h after treatment and luciferase activity was measured using Luciferase Assay System (Promega, Leiden, The Netherlands). Infections were performed using pseudotyped particles obtained by co-transfecting HXB2 Env with pNL4.3.Luc.R-E-plasmid into HEK 293T cells using PEI ...
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bioRxiv - Physiology 2020Quote: ... Cells were harvested 6 h after stimulation and luciferase activity was measured according to the manufacturer’s protocol (Promega, Madison, WI). Relative luciferase activity was normalized to the β-galactosidase activity measured using multi-mode plate reader (SpectraMax M5 ...
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bioRxiv - Molecular Biology 2020Quote: ... Apoptosis was assessed by incubating the cells with 200nM Staurosporin or medium for 4 h and the caspase 3/7 activity was assayed using the ApoOne Caspase 3/7 Assay (Promega). Senescence associated β-Galactosidase activity was analyzed with the Senescence Associated β-Galactosidase Staining Kit (Cell Signalling Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were washed twice with UA buffer and twice with 50mM ammonium bicarbonate prior to digest of the immobilized proteins on the filter for 2 h at RT using 1 mg Lys-C (Wako Chemicals) and for 16 h at 37C using 2 mg trypsin (Promega). Tryptic peptides were collected by centrifugation (10 min at 14,000 g) ...
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bioRxiv - Immunology 2019Quote: ... Transductions were performed in triplicate on A549 cells in 96 well plates and 48 h later cells were lysed with 5x Passive lysis buffer (Promega), then added to luciferase substrate and luciferase activity measured on an Orion L Microplate Luminometer (Berthold Detection Systems).
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bioRxiv - Neuroscience 2021Quote: ... or methadone were added for another 10 min followed by addition of 20 μL of the RLuc substrate coelenterazine h (Promega), at 5 μM final concentration ...
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bioRxiv - Zoology 2021Quote: ... The cells transfected with different recombinant vectors were cultured for additional 36 h at 37 °C for transcriptional activity analysis by using the Dual-Glo Luciferase Assay System (Promega) with a luminometer (Promega ...
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bioRxiv - Immunology 2021Quote: ... The mixture was subsequently incubated with 293TAce2 cells for 48 h after which cells were washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Biophysics 2021Quote: ... were plated into six-well tissue culture plates containing collagen-coated glass cover slips 12 to 24 h before being transfected with 1 μg of the indicated plasmid DNA using FuGENE 6 transfection reagent (Promega) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... Transfected cells were assayed after 48 h (as indicated for firefly and Renilla luciferase activity using the Dual-Luciferase Reporter Assay System (Promega) in a GloMax® Explorer System (GM3500).
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bioRxiv - Microbiology 2021Quote: ... LucNeo#2 cells were treated with the compounds indicated in Figure 3C for 48 h and the luciferase activity was measured with Luciferase Assay System kit (Promega). Sofosbuvir ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed at 36 h after transfection and the luciferase activity was analyzed using the Dual-Luciferase Reporter Assay Kit (Promega).
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bioRxiv - Immunology 2021Quote: ... After 48-72 h luminescence was assessed as a proxy of infection by lysing cells with the Bright-Glo luciferase kit (Promega), using a Glomax plate reader (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... and proteins were sequentially digested with LysC (1:50, w/w; FUJIFILM Wako) for 3 h at 37 °C and trypsin (50:1, w/w; Promega) overnight at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... At 48 h later the cells were washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... proteins were reduced by dithiothreitol and alkylated by iodoacetamide in the dark at room temperature, followed by in-solution digestion sequentially using lysyl endopeptidase (Lys-C, Wako) for 3 h and trypsin (Promega) overnight at room temperature as previously described (Paul et al. ...
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bioRxiv - Genomics 2021Quote: ... 8 μg of RNA were treated for 1 h at 37°C with 12 units of RQ1 DNase (Promega®) followed by phenol-chloroform extraction and ethanol precipitation of RNA which was subsequently dissolved in water ...
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bioRxiv - Neuroscience 2021Quote: ... The transfected cells were then treated with Aβ42 (1-4 μM) or calcitriol (100 nM) for 6 h before luciferase activity assay (Dual-Luciferase Reporter Assay, Promega). A 587-bp region of the human Cyp24a1 gene promoter that contains two VDRE motifs was cloned into the promoter-less luciferase expression vector pGL3-basic (Promega ...
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bioRxiv - Genomics 2022Quote: ... Caspase 3/7 activity was measured 16 and 24 h later using the Caspase-Glo 3/7 Assay System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Luciferase activity was detected 24 h after transfection and normalized to Renilla luciferase activity using the Dual-Glo Luciferase system (Promega). The relative luciferase unit was defined as the ratio of Firefly versus Renilla ...
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bioRxiv - Evolutionary Biology 2022Quote: ... or RepNDK-RNA was incubated at 37 °C for 12 h in a translation system and FluoroTect GreenLys tRNA (Promega), with neither CTP nor CDP to preclude RNA replication ...
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bioRxiv - Physiology 2022Quote: ... Denatured proteins were then enzymatically digested into peptides upon incubation first with endoproteinase LysC (Wako Laboratories) at 25 °C shaking for 2 h and then with sequencing-grade trypsin (Promega) at 25 °C shaking overnight ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were analyzed 24 h after transfection to measure firefly and renilla luciferase activities using GloMax 96 microplate luminometer (Promega).
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bioRxiv - Microbiology 2022Quote: ... Luciferase reporter gene expression was quantified 60-72 h after infection upon lysis and the addition of Bright-GloTM Luciferase substrate (Promega). Data were retrieved from a BioTek microplate reader with Gen 5 software ...
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bioRxiv - Molecular Biology 2023Quote: ... For ACE2 overexpression HEK293T cells were transfected for 24 h with human ACE2 (pCG1-hACE2, 200 ng DNA/well) using JetPrime (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were harvested 24 h of post-transfection and RLuc and FLuc activities were measured by Dual Glo luciferase assay (Promega), according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2022Quote: ... and luciferase activity was measured after 48 h using the dual-luciferase reporter assay system and GloMax 20/20 (Promega). pRL-null Renilla plasmid was cotransfected at 0.4 pg/μL to control for transfection efficiency ...
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bioRxiv - Immunology 2023Quote: ... Luciferase and Renilla signals were measured 48 h after transfection by a Dual-Luciferase Reporter Assay Kit (Promega, No. E1980). Data were analyzed and normalized by the division of firefly luciferase activity with that of Renilla luciferase to eliminate transfection efficiency difference.
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bioRxiv - Microbiology 2022Quote: ... allowing to adhere for 24 h before addition of a serial dilution of inhibitor and measurement of cell viability 72 h later using the CellTiter AQueous One solution (Promega), following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Protein was then digested with 2.5 μg Lys-C (Wako, Japan) for 4 h at 37°C and 2 μg trypsin (Promega, V5113) for an additional 4 h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... allowing to adhere for 24 h before addition of a serial dilution of protease inhibitors and measurement of cell viability 72 h later using the CellTiter AQueous One solution (Promega), following manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... followed by retrotranscription to cDNA with M-MLV RT, RNase H(–) point mutant (Promegain presence of buffer, nucleotides and RNasin Plus RNase Inhibitor (Promega). Reaction was then incubated at 40 °C for 10 min ...