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Citations for Promega :
651 - 700 of 1243 citations for Rabbit Anti Sheep IgG H+L FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured for 24 h with a complex of DNA and FuGENE® HD Transfection Reagent (#E2311; Promega) at a ratio of 1:3 prepared in Opti-MEM reduced serum medium (#31985070 ...
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bioRxiv - Molecular Biology 2023Quote: ... The released peptides were then transferred over to a new tube and a 4 h digest using 0.5 ug mass spectrometry-grade trypsin (V5111, Promega) at 37 C for 4 hours was conducted ...
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bioRxiv - Molecular Biology 2023Quote: ... After incubation at 37 °C/5 % CO2 for 20 h 40 nl HaloTag® NanoBRET™ 618 Ligand (PROMEGA) was added to the cells using an Echo acoustic dispenser (Labcyte ...
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bioRxiv - Microbiology 2023Quote: ... The virus infectivity was determined 48 h later by quantifying the luciferase activity (luciferase assay system, Promega, Madison, WI). Neutralization curves were normalized to virus only control ...
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bioRxiv - Immunology 2023Quote: ... All conditions used were tested for cytotoxicity by measuring lactate dehydrogenase in the conditioned media after 24 h incubation according to the manufacturer’s instructions (CytoTox96, Promega) (Figure S1).
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bioRxiv - Cell Biology 2023Quote: ... Proteins were proteolysed for 2 h at room temperature using 0.5 μg Lys-C (Wako) and subsequently for 16 h at 37 °C using 1 μg trypsin (Promega). Peptides were collected by centrifugation and acidified with 0.5 % trifluoroacetic acid.
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bioRxiv - Biochemistry 2023Quote: ... Beads were resuspended in 500 µL of 100 mM Tris-HCl pH 8.0 containing 2 mM CaCl2 and digested at 37 °C for 1 h with 0.2 μg of trypsin/LysC (#V5073 Promega). The samples were then loaded onto homemade SepPak C18 Tips packed by stacking three AttractSPE disk (#SPE-Disks-Bio-C18–100.47.20 ...
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bioRxiv - Immunology 2023Quote: ... the cells plus supernatants were combined with an equal volume of the respective caspase-Glo reagent for 1 h according to the manufacturer’s instructions (Promega). The mixture was then transferred to a Lumitrac 600 plate and luminescence was measured using a SpectraMax i3 instrument and the SoftMax Pro 6.3 Software (Molecular Devices) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Probe synthesis reactions were performed at 37 °C for 3 h and then were treated with DNase I (Promega) at 37 °C for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were seeded onto acid-washed coverslips at a density of 3 × 104 cells per coverslip for 24 h before transfection with 1μg of plasmid DNA using 3 μl Fugene HD transfection reagent (Promega). HaloTagged AP-2 σ2 was visualized by adding Janelia Fluor 646-HaloTag ligand (Promega) ...
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bioRxiv - Developmental Biology 2023Quote: ... Firefly and Renilla luciferase activities were measured 24 h after transfection with the Dual-Glo luciferase assay system (Promega) using a Luminometer (Berthold Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... was added at the indicated concentrations for 48 h and analyzed using the MTT reagent according to the manufacturer’s instructions (Promega). The graph represents the comparison with the control group ...
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bioRxiv - Microbiology 2023Quote: ... OD600nm was monitored every hour at 37°C for 24 h by using a GloMax Explorer plate-reader (Promega).
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bioRxiv - Cell Biology 2023Quote: ... 72 h later cell viability was measured using CellTiter-Glo® Luminescent Cell Viability Assay reagent (Promega, Cat. # G7572) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... the medium was removed and substituted with OptiMEM media supplemented with 4 µM coelenterazine-H (Promega; Cat. No. S2001), and incubated for a period of 2 to 3 hours ...
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bioRxiv - Cell Biology 2020Quote: ... GFP-MICAL1 and GFP-MICAL2 stable cells were transfected with pE/L-LifeAct-iRFP670 using FUGENE (Promega) and infected with vaccinia virus as previously described (35) ...
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bioRxiv - Microbiology 2021Quote: ... Firefly luciferase reporter activity was measured by incubating ∼2x105 cells in 25 L lysis buffer (Promega, E1531) for 5 min while orbital shaking and using 20 μL of lysate with 20 μL of Luciferase Kit substrate (Promega ...
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bioRxiv - Cell Biology 2022Quote: To measure the level of L-Lactate secretion by MSCs/ MCF7s the Lactate-GloTM Assay (Promega, J5021) was employed ...
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bioRxiv - Biophysics 2021Quote: ... cells were grown on glass coverslips for 24 h and then transfected with both mCherry-lamin B1-10 and GFP-connexin-43 using ViaFect (Promega). The mCherry-lamin B1-10 was a gift from Michael Davidson (Addgene plasmid #55069) ...
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bioRxiv - Molecular Biology 2021Quote: ... FASP was carried out in two 10 kDa MWCO filters with a 50 mM iodoacetamide alkylation step and proteins were digested in 2M urea with 2% wt/wt Lys-C (Wako) for 6 h and 2% modified trypsin (Promega) for 12 h at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were subjected to digestion of Lys-C (Wako Chemicals) (1:50) for 2 h at RT and then to trypsin (Promega) (1:50 ...
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bioRxiv - Cell Biology 2022Quote: ... two micrograms of RNA from each sample were reverse transcribed at 42 °C for 1 h followed by 95 °C for 10 min M-MLV Reverse Transcriptase (Promega) and oligo(T)18 primers (Takara) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were lysed 24 h after transfection and luciferase activities were measured with the Dual-Luciferase Reporter Assay System (Promega) in a GloMax 20/20 luminometer (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... Infection rates were assessed by measuring luciferase activity in cell lysates at 16 h post transduction with a commercially available kit (Promega). Values for untreated controls were set to 100% infection.
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bioRxiv - Genomics 2020Quote: ... A double-stranded DNA adapter with a T-overhang was ligated for 1 h at 20°C and the resulting products were bead purified (ProNex, Promega), eluted and then split into two identical aliquots ...
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bioRxiv - Genomics 2020Quote: ... After 48 h Luciferase assay was performed according to manufacturers’ instruction using the Dual-Luciferase Reporter Assay System Kit (Promega).
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bioRxiv - Plant Biology 2020Quote: ... Reverse transcription was achieved with 4 µg of RNAs in the presence of Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase (RNase H minus, Point Mutant, Promega) after annealing with an anchored oligo(dT)18 primer as described (Wirth et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were then stimulated with 1 ng/mL of IL-1β for 6 h and subsequently washed with ice-cold PBS and lysed in Passive Lysis Buffer (PLB; Promega). Luciferase activity was measured in a Clariostar plate reader (BMG Biotech ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were also reverse-transfected in identical manner to determine cell viability 72 h post-transfection using CellTiter-Glo (Promega) following manufacturer’s recommendations.
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bioRxiv - Systems Biology 2019Quote: ... The sample was digested for 16 h at 37C using a 1:50 (w/w) ratio of Sequencing Grade Modified Trypsin (Promega):protein in the gel ...
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bioRxiv - Immunology 2019Quote: ... Expression of RLuc fusion proteins was estimated by measuring the luminescence of the cells after incubation with 5 µM coelenterazine H (Promega). In parallel ...
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bioRxiv - Biochemistry 2019Quote: ... The washed beads were resuspended in 200 µl of digestion buffer and incubated for 5 h with 1 µg of trypsin (Promega) at 37°C ...
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bioRxiv - Systems Biology 2019Quote: ... Cultures were harvested 24 h post-transfection and processed for firefly and Renilla luciferase activity using the Dual-Luciferase Reporter Assay System (Promega).
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bioRxiv - Synthetic Biology 2019Quote: ... Backbone and inserts were ligated either for 3 h at RT or overnight at 4°C using T4 ligase (Promega). All vector sequences (Table S5 ...
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bioRxiv - Bioengineering 2020Quote: ... Luminescence from the activity of luciferase was measured 48 h post-infection using a TECAN infinite M200 pro plate reader after applying Bright-Glo reagent (Promega) on cells.
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bioRxiv - Molecular Biology 2021Quote: ... The sequences ligated by the T4 ligase at 4 °C for 12 h were cloned in the pGEM T-easy vector (Promega). The competent Escherichia coli X10-Gold (Stratagene ...
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bioRxiv - Neuroscience 2021Quote: ... The messenger RNAs (mRNAs) contained in 1 μg of purified RNA extracts were reverse-transcribed using the reverse transcriptase RNase H minus (Promega) and oligo-d(T)15 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were lysed 24 h after chemical exposure to measure luciferase activity (Firefly luciferase assay system; Promega Madison WI, USA) and β-galactosidase activity ...
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bioRxiv - Cancer Biology 2020Quote: ... grown for 48 h and then caspase 3/7 enzymatic activity determined using Apo-One Homogenous caspase 3/7 activity assay kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... R-Luc and F-Luc activities were measured 48 h after transfection using the Dual-Luciferase Reporter Assay System (Promega). Renilla luciferase activity was normalized to firefly luciferase.
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bioRxiv - Biochemistry 2021Quote: ... 55 mM IAA at room temperature for 30 min in the dark and digested at 37°C for 16 h with 2 μg porcine trypsin (sequencing grade, Promega). The resultant tryptic peptides were extracted in 40% ACN/0.1% TFA into fresh Protein LoBind® microtubes ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of RNA was converted into cDNA with M-MLV RNase H–Point Mutant reverse transcriptase (Promega Corp., USA) and an anchored oligo dT21 primer (Metabion ...
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bioRxiv - Plant Biology 2020Quote: ... and then reverse-transcribed with a cox1 specific primer (Atcox1-1; table S1) using M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RNase H minus, point mutant; Promega). The RNA template was degraded by adding 1/5 volume of 1 M NaOH to the reaction mixture and incubating at room temperature for 10 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... The soluble fraction was then digested for 20 h at 37°C using a 1:50 ratio of trypsin (Promega) to extracted protein while gently shaking ...
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bioRxiv - Microbiology 2020Quote: ... post-transfection with or without OSM (10 ng/mL for 8 h) or IFN-α (1,000 U/ml for 16 hours) before lysis using Passive Lysis Buffer (Promega). Firefly and Renilla luciferase activity was then determined in a dual luciferase assay ...
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bioRxiv - Microbiology 2021Quote: Epithelial cytotoxicity was determined by quantification of lactate dehydrogenase activity in A549 culture supernatants at 24 h post fungal challenge using the Cytox 96 Non-Radioactive Cytotoxicity Assay kit (Promega) according to the manufacturer’s protocol and using recombinant porcine LDH enzyme (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... phaffii cells were cultured for 12-14 h and total RNA was isolated using RNA isolation kit (Cat. # Z3100, Promega) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Infected SupT1 cells were incubated for 48 h and lysed in 100 μL of Bright-Glo Luciferase Reagent (Promega, E2610). Infection was assessed by luciferase activity using a LUMIstar Omega microplate luminometer (BMG Labtech) ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... Proteins were digested with endoproteinase LysC (Wako Laboratories) for 2 h at 25 °C followed by overnight digest with sequencing-grade trypsin (Promega) at 25 °C (enzyme-to-substrate ratios of 1:50) ...
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bioRxiv - Cell Biology 2020Quote: ... Luciferase activity was measured 60 h post-transfection using the Dual-Luciferase® Reporter Assay System (Promega GmbH, Mannheim, Germany) on an Infinite® M200 Pro (TECAN ...