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Citations for Promega :
601 - 650 of 1243 citations for Rabbit Anti Sheep IgG H+L FITC since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was determined after 72 h using the CellTiter-Glo® luminescent assay (Promega, catalog number G9242). Cell viability of DMSO-treated cells was set to 100% ...
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bioRxiv - Genomics 2023Quote: ... Cells were lysed 24 h after transfection and assayed with the Dual-Luciferase Reporter assay kit (Promega, E1960).
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured 72 h after drug treatment using CellTiter-Glo 2.0 Cell Viability Assay (Promega, G9241) following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Relative luciferase activity was measured 24 h post-transfection with the Dual-Glo Luciferase Assay System (Promega, USA).
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with LDLR-GFP (WT, C75W, and C75W/C34W) for 48 h with Fugene 6 (Promega). Cells were starved in 5% LPDS media (Millipore Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... and incubated for 1 h at room temperature before measuring luminescence using a GloMax Explorer plate reader (Promega). A standard curve was used for quantification of the UDP produced ...
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bioRxiv - Microbiology 2024Quote: ... The cells were collected at 48 h post-infection and virus infectivity were measured by luciferase assay (Promega).
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bioRxiv - Cancer Biology 2024Quote: ... Luciferase intensity was recorded 48 h after transfection using the dual-luciferase reporter assay system (Promega, Madison, WI).
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assessed at 72 h post-treatment by performing CellTiter-Glo Luminescent Cell Viability Assays (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Intracellular and extracellular L-lactate levels were measured using Lactate-Glo™ Assay Assay Kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... 0.2 μl Promega GoTaq and 12.6 μ l DNase free water (Promega catalog # M3005, Madison WI). Samples were denatured for 4 minutes at 95°C then underwent 32 cycles of PCR (95° C for 30 seconds ...
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bioRxiv - Immunology 2021Quote: ... and luminescence was quantified using SpectraMax L (Molecular Devices. 100 μL Luciferase Assay Reagent (Promega E1483) luciferin/well ...
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bioRxiv - Cell Biology 2019Quote: ... CYP3A4 and CYP2C9 activities were measured by incubating cultures with luciferin-IPA or luciferin-H substrates (Promega, Madison, WI) for 1 or 3 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... the RNA/oligonucleotide mixture was diluted to 30 µl final with a reaction mix (1X RNase H reaction buffer, 65 µM DTT, 0.5U/µl RNasin (Promega), 50 U RNase H (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... Fujifilm) and then incubated for 8 h at 37 °C with sequence-grade trypsin (1□g; Promega, Madison, Wisconsin). Peptides were desalted on Ultra Micro Spin Columns C18 (The Nest Group Inc. ...
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bioRxiv - Cancer Biology 2019Quote: ... and cultured for 72 h at 37°C before viability assay using the ATP-based CellTiter-Glo (CTG) 2.0 Assay (Promega). Luminescence was read with the LMAX II luminometer (Molecular Devices) ...
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bioRxiv - Bioengineering 2020Quote: ... The transduction efficiency was quantified at 16 h posttransduction by measuring firefly luciferase activity according to the manufacturer’s instructions (Promega).
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ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... Five micrograms of RNA were reverse transcribed at 42 °C for 1 h using M-MLV Reverse Transcriptase (Promega) and oligo(T)18 primers (Takara) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were collected 24 h after transfection and luciferase activities were measured with Dual-Luciferase Reporter Assay System (Promega) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed 24 h later and subjected to luciferase assays using the Dual-Luciferase Reporter Assay System (Promega).
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bioRxiv - Systems Biology 2021Quote: ... Protein digestion was performed for 16 h at 37 °C with 0.1 µg of Trypsin/Lys-C mix (1 µL of stock; Promega) and stopped by lowering pH to 3 by the addition of 5 µL of 10% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmids were transfected into HeLa cells for 24 h (2 μg of cDNA/9.6 cm2 plate) using Fugene (Promega). For gene silencing ...
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bioRxiv - Cell Biology 2020Quote: ... at a protein:Lys-C ratio of 100:1 (w/w) for 4 h at 37°C followed by trypsin (Promega) digestion at a ratio of 50:1 (w/w ...
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bioRxiv - Biophysics 2022Quote: ... and resuspended in assay buffer (HBSS with 20 mM HEPES pH 7.45) with 1 μg/mL freshly prepared coelenterazine h (Promega). Cells were plated in white-walled ...
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bioRxiv - Biochemistry 2022Quote: ... The pellet was resuspended in 0.1M Triethylammonium bicarbonate (TEAB) and overnight (∼16 h) digestion was performed with trypsin/Lys-C mix (1:25 protease:protein; Promega) at 37° C with constant mixing using a thermomixer ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were lysed after 72 h (2+1 DIV for differentiations) using the Dual Luciferase Reporter kit (Promega, E1960) and luciferase activity was measured using a Victor3 (Perkin Elmer ...
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bioRxiv - Plant Biology 2021Quote: The RNA was transcribed into cDNA using the M-MLV Reverse Transcriptase (RNase H- Point Mutant, Promega, Walldorf, Germany). qPCR was performed using a Realplex Mastercycler system (Eppendorf ...
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bioRxiv - Neuroscience 2021Quote: ... Dose response curve for H2O2 was performed 24 h after the H2O2 treatment by using CellTiter Blue (Promega, G8080). CellTiter blue reagent (diluted 1:4 with PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Luciferase assays were carried out at 48 h after infection following the manufacturer’s instructions with the Dual-luciferase Reporter Assay System (E1910; Promega). Firefly luciferase activity was normalized by Renilla luciferase activity.
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bioRxiv - Microbiology 2021Quote: ... Cell viability was determined 24 h after the addition of compound-containing DMEM using the CellTiter-Glo kit (Promega) and a luminescence plate reader (Perkin Elmer) ...
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bioRxiv - Cell Biology 2020Quote: ... The sample was first treated by 2.5 μg of LysC (Wako) for 3 h at 37 °C with shaking and then treated with 2.5 μg of Trypsin (Promega) for over-night at 37 °C with shaking ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were harvested 24 h post transfection and luciferase assay was performed as per the manufacturer’s protocol (Promega, E2610). Readings for luminescence were taken in luminometer (Berthold ...
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bioRxiv - Microbiology 2021Quote: ... Dual luciferase reporter assays were performed 48 h post transfection using the Dual Luciferase Reporter Assay System following the manufacturer’s protocol (Promega). The renilla (primary reporter ...
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bioRxiv - Molecular Biology 2021Quote: ... Digestion was performed for 8 h at 37°C using 70 ng modified porcine trypsin (Promega, Fitchburg, WI, USA). Tryptic peptides were extracted using 70% acetonitrile and dried using a SpeedVac vacuum concentrator.
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bioRxiv - Microbiology 2020Quote: ... Samples were digested initially with LysC (WAKO) at 37°C for 2.5 h and subsequently with 10 μg trypsin (modified sequencing grade, Promega) overnight ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized by reverse transcription using RevertedAid H minus reverse transcriptase (ThermoFischer Scientific) and random hexamer primers (Promega), in combination with a primer targeting the 3’ end of the MERS-CoV genome ...
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bioRxiv - Molecular Biology 2022Quote: ... The proteins were digested for 2 h at RT using 0.5 μg Lys-C (Wako Chemicals) and for 16 h at 37 °C using 1 μg trypsin (Promega). After centrifugation (10 min at 14 000 g) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were collected at 24 h after transfection and luciferase activity was measured with the Dual-Luciferase Assay (Promega) with a Luminoskan Ascent luminometer (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: Cells transfected with corresponding plasmids for 24 h were applied to cytotoxicity test using CytoTox 96® Reagent (Promega) according to the manufacturer’s manual ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured for 24 h with a complex of DNA and FuGENE® HD Transfection Reagent (#E2311; Promega) at a ratio of 1:3 prepared in Opti-MEM reduced serum medium (#31985070 ...
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bioRxiv - Molecular Biology 2023Quote: ... The released peptides were then transferred over to a new tube and a 4 h digest using 0.5 ug mass spectrometry-grade trypsin (V5111, Promega) at 37 C for 4 hours was conducted ...
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bioRxiv - Molecular Biology 2023Quote: ... After incubation at 37 °C/5 % CO2 for 20 h 40 nl HaloTag® NanoBRET™ 618 Ligand (PROMEGA) was added to the cells using an Echo acoustic dispenser (Labcyte ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were seeded onto acid-washed coverslips at a density of 3 × 104 cells per coverslip for 24 h before transfection with 1μg of plasmid DNA using 3 μl Fugene HD transfection reagent (Promega). HaloTagged AP-2 σ2 was visualized by adding Janelia Fluor 646-HaloTag ligand (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... The virus infectivity was determined 48 h later by quantifying the luciferase activity (luciferase assay system, Promega, Madison, WI). Neutralization curves were normalized to virus only control ...
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bioRxiv - Immunology 2023Quote: ... All conditions used were tested for cytotoxicity by measuring lactate dehydrogenase in the conditioned media after 24 h incubation according to the manufacturer’s instructions (CytoTox96, Promega) (Figure S1).
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bioRxiv - Cell Biology 2023Quote: ... Proteins were proteolysed for 2 h at room temperature using 0.5 μg Lys-C (Wako) and subsequently for 16 h at 37 °C using 1 μg trypsin (Promega). Peptides were collected by centrifugation and acidified with 0.5 % trifluoroacetic acid.
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bioRxiv - Biochemistry 2023Quote: ... Beads were resuspended in 500 µL of 100 mM Tris-HCl pH 8.0 containing 2 mM CaCl2 and digested at 37 °C for 1 h with 0.2 μg of trypsin/LysC (#V5073 Promega). The samples were then loaded onto homemade SepPak C18 Tips packed by stacking three AttractSPE disk (#SPE-Disks-Bio-C18–100.47.20 ...
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bioRxiv - Immunology 2023Quote: ... the cells plus supernatants were combined with an equal volume of the respective caspase-Glo reagent for 1 h according to the manufacturer’s instructions (Promega). The mixture was then transferred to a Lumitrac 600 plate and luminescence was measured using a SpectraMax i3 instrument and the SoftMax Pro 6.3 Software (Molecular Devices) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Probe synthesis reactions were performed at 37 °C for 3 h and then were treated with DNase I (Promega) at 37 °C for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... 72 h later cell viability was measured using CellTiter-Glo® Luminescent Cell Viability Assay reagent (Promega, Cat. # G7572) according to the manufacturer’s protocol ...