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Citations for Promega :
101 - 150 of 1305 citations for Purified Zika Virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... the protein lysates were digested with trypsin (Promega, Madison, WI) at 37°C overnight and the digested samples were desalted with 1cc C18 Sep-Pak solid phase extraction cartridges (Milford ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were prepared in 1 × Reporter lysis buffer (Promega, E2510), and Luciferase assays were performed according to the manufacturer’s protocol (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... In vitro translation was performed in rabbit reticulocyte lysate (Promega) supplemented with 1 mCi/ml [35S]Met/Cys ...
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bioRxiv - Microbiology 2021Quote: Translation was performed in Flexi® Rabbit Reticulocyte Lysate (Promega) supplemented with amino acid mixture minus methionine and leucine ...
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bioRxiv - Molecular Biology 2022Quote: A rabbit reticulocyte lysate in vitro translation kit (Promega, L4960) was coupled to in vitro transcription with the supplementation of MgCl2 ...
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bioRxiv - Microbiology 2023Quote: Cell lysate was prepared by using passive lysis buffer (Promega). Firefly and renilla luciferase activities (Fluc and Rluc ...
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bioRxiv - Biochemistry 2023Quote: ... with the T7 TNT Coupled Reticulocyte Lysate System (Promega, L1170). Each substrate was amplified with primers by PCR (using template plasmids from the hORFeome collection or commercially available plasmids ...
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Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translationbioRxiv - Biochemistry 2024Quote: ... Lysates were mixed with Firefly luciferase assay reagent (E1500 Promega) as per manufacturer’s protocol and scanned for luminescence in Tecan scanner.
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bioRxiv - Cell Biology 2020Quote: 1 µg of total RNAs were reverse transcribed with Moloney murine leukemia virus Reverse Transcriptase (M170B, Promega). 100 ng cDNA and FastStart Universal SYBR Green Master (04913850001 ...
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bioRxiv - Immunology 2021Quote: ... and reversed transcribed with random hexamers (IDT) using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega) according to the manufacturers’ instructions ...
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bioRxiv - Microbiology 2021Quote: ... Virus -specific PCR products were cloned into pGEM-T Easy Vector (A1360, Promega Co., Madison, WI, USA) and used to transform E ...
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bioRxiv - Immunology 2021Quote: ... cDNA synthesis was performed using Moloney Murine Leukemia Virus Reverse Transcriptase (MML-V)(Promega Corporation, Madison, WI). ViiA-7 instrument (Life Technologies ...
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bioRxiv - Genomics 2022Quote: ... Reverse transcription was carried out using moloney-murine leukemia virus (M-MLV) reverse transcriptase kit (Promega, USA). Reverse transcription was completed in a 30 µL final volume reaction containing 400 U M-MLV and 1 mM dNTP mix (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... and 0.25 μg vesicular stomatitis virus G glycoprotein expression plasmids using the Fugene 6 transfection reagent (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... and translated using the nuclease-treated rabbit reticulocyte lysate (RRL; Promega). Typical reactions were comprised of 75% v/v RRL ...
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bioRxiv - Cell Biology 2021Quote: ... the mRNA was translated in a Flexi rabbit reticulocyte lysate (Promega) containing 35S methionine/cysteine (Perkin Elmer ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were analyzed using a Dual-Luciferase Reporter Assay System (Promega) with a Tecan Microplate Reader (Tecan Infinite M1000) ...
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bioRxiv - Molecular Biology 2020Quote: ... which was synthesized in vitro with reticulocyte lysate systems (Promega, L5020), for 2 h at room temperature in binding buffer (50 mM Tris-Cl ...
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bioRxiv - Microbiology 2019Quote: ... and the lysate was treated with 20U/ml RNase A (Promega) and 60U/ml RQ1 DNase (Promega) ...
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bioRxiv - Biochemistry 2021Quote: ... The acquired mRNA was then translated in rabbit reticulocyte lysates (Promega) containing 35S- labelled Met and Cys ...
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bioRxiv - Immunology 2020Quote: ... Dual luciferase activity in the total cell lysates was quantified (Promega).
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bioRxiv - Microbiology 2020Quote: ... The cell lysates were treated with RNAse free DNAse (Promega Corp) for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... mRNA transcripts were then translated in rabbit reticulocyte lysate (RRL) (Promega) in the presence of L-35S-methionine (PerkinElmer ...
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bioRxiv - Molecular Biology 2022Quote: ... and in vitro translated with a Rabbit Reticulocyte Lysate system (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were then subjected to Dual-Luciferase Reporter assay (Promega) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... 15 μl reactions contained 60% nuclease-treated rabbit reticulocyte lysate (Promega), 20 μM amino acid mixture minus leucine ...
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bioRxiv - Plant Biology 2020Quote: ... and cDNA synthesized using Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and universal Random hexamers (Promega). qRT-PCR was performed with 2x PowerUP™ SYBR™Green Master Mix in the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription (RT) of different HCV genomic regions was performed using avian myeloblastosis virus (AMV) reverse transcriptase (Promega), and subsequent PCR amplification was carried out using AccuScript (Agilent Technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 µg of total RNA to cDNA using Oligo(dT)15 primers (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... virus was removed and cells were transfected with 2 µg of plasmid DNA pUCSP-A24Rcd using FuGeneHD (Promega) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... A total of 1.5 μg RNA was reversely transcribed using Moloney murine leukemia virus reverse transcriptase (M1701, Promega) for 90 min at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Complementary DNA (cDNA) was synthesized using M-MLV (Moloney murine leukemia virus) reverse transcriptase (Promega, Madison, WI, USA), and amplified using SYBR Green qRT-PCR SuperMix-UDG reagents (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... virus-containing media was removed and replaced with 35 μl of 1x Passive lysis buffer (Promega; Ref# E194A). Plates underwent three freeze-thaw cycles consisting of freezing on dry-ice for 15 minutes followed by thawing at 37°C for 15 minutes ...
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bioRxiv - Microbiology 2021Quote: ... virus inoculum was removed and cells were then transfected with 2 µg of plasmid DNA using FuGeneHD (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Isolated RNA (1 μg) was used to synthesize cDNAs using Murine Moloney Leukemia Virus reverse transcriptase (MMLV, Promega). Subsequently ...
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bioRxiv - Microbiology 2022Quote: ... The reverse transcription (RT) mix contained 200 units of recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase (Promega), 20 units of RNAsin (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... reverse transcription was performed with 0.5 μl (corresponding to 5 U) of Avian Myeloblastosis Virus Reverse Transcriptase (Promega), 0.1 μl dNTP mix (10 mM) ...
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bioRxiv - Immunology 2023Quote: ... 2 μg RNA was used to synthesize first-strand cDNA using Moloney mouse leukemia virus (M-MLV) (Promega). The amplification of specific PCR products was detected using SYBR Premix Ex TaqTM II (Vazyme) ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 1.5 µg RNA was reversely transcribed using 0.5 μl Moloney murine leukemia virus reverse transcriptase (M1701, Promega), 4 µl RT buffer 5x (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... The cells were collected at 48 h post-infection and virus infectivity were measured by luciferase assay (Promega).
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bioRxiv - Genetics 2019Quote: ... Purified recombinant luciferase protein (Promega) was used to generate a standard curve.
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bioRxiv - Bioengineering 2021Quote: ... PCR product was purified (Promega) and digested using DpnI (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... with purified LgBiT Protein (Promega). Briefly ...
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bioRxiv - Cell Biology 2019Quote: ... Translations were performed using the Flexi® Rabbit Reticulocyte Lysate System (Promega) supplemented with DTT (10 mM ...
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bioRxiv - Neuroscience 2021Quote: mRNAs were in vitro translated with Flexi Rabbit Reticulocyte Lysate System (Promega), as previously described (23 ...
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bioRxiv - Biochemistry 2019Quote: Translations were performed using the Flexi® Rabbit Reticulocyte Lysate System (Promega) supplemented with 10 mM DTT (Melford ...
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bioRxiv - Biochemistry 2020Quote: ... Lep variants were then translated using rabbit reticulocyte lysate (Promega, Madison, WI) supplemented with canine rough microsomes (tRNA probes ...
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bioRxiv - Molecular Biology 2021Quote: In vitro translation in RNase-treated Rabbit Reticulocyte Lysate System (RRL, Promega) was carried out as recommended by the manufacturer with slight modifications ...
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bioRxiv - Molecular Biology 2019Quote: ... reactions were prepared as per Rabbit Reticulocyte Lysate System (Promega Cat#L4960). 10 µL of reaction mixture was supplemented with 1 µg of either Renilla or Firefly luciferase mRNA alongside recombinant GST or GST-PKM1 ...
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bioRxiv - Molecular Biology 2021Quote: Messenger RNAs were translated in nuclease-treated rabbit reticulocyte lysate (RRL) (Promega). Typical reactions were composed of 90% (v/v ...