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Citations for Promega :
401 - 450 of 3328 citations for Polybrominated Diphenyl Ether Predominant Congener Mixture 1 100 Dilution Unlabeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 170 µL medium was removed and 30 µL caspase 3/7 reaction (1:100 Z-DEVD-R110 fluorogenic substrate:Homogenous buffer; ApoONE, Promega) was added to each well ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.05% (v/v) NP-40 and 0.5% (v/v) Triton X-100) containing 20 U ml−1 of RNasin (Promega), 1 mM DTT ...
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bioRxiv - Cell Biology 2024Quote: ... Each sample was digested on beads in 100 µl of 50 mM ammonium bicarbonate with 1 µg trypsin (Promega) in spin cap tubes sitting in fresh mass-spectrometry grade Eppendorf tubes ...
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bioRxiv - Molecular Biology 2019Quote: ... 100X dilution of renilla luciferin (Promega, E2920 Dual-Glo® Stop & Glo® Reagent), or 10,000X dilution of NanoLuc substrate (Promega ...
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bioRxiv - Cell Biology 2022Quote: ... raw luminescence values obtained by treating serial dilutions of pure UDP or GDP (Promega) with the nucleotide detection reagent for 60 minutes were used to plot a standard concentration curve ...
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bioRxiv - Cell Biology 2022Quote: ... Dilution with Tris 50mM was done before the addition of trypsin 500 ng (Promega) and digestion at 37°C for 8h ...
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bioRxiv - Biochemistry 2023Quote: ... Twenty-five µl of 100x dilution of NanoBRET NanoGlo substrate (Promega, Madison, MA, USA) was added per well ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were split and half were re-plated to propagate the cultured mixtures while the other half were subjected to Dual-Glo Luciferase Assay (Promega) to set baseline signal ...
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bioRxiv - Microbiology 2022Quote: ... The amplification was performed in 25 μl mixtures containing 12.5 μl GoTaq□Green Master Mix (Promega, Madison, Wl, United States), 0.625 μl of each primer (20mM) ...
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bioRxiv - Microbiology 2022Quote: ... The amplification was performed in 50 μl mixtures containing 20 μl GoTaq□Green Master Mix (Promega, Madison, Wl, United States), 1 μl of each primer (20 mM) ...
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bioRxiv - Molecular Biology 2020Quote: ... Equal amounts of RNA (1 μg) were reverse-transcribed with a mixture of oligo(dT)15 and random hexamer primers using M-MLV polymerase (Promega). RT-qPCR gene expression quantifications were performed using AceQ qPCR SYBR Green Mix (Vazyme Biotech ...
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bioRxiv - Physiology 2019Quote: ... The concentrated urea was diluted 12 times with 50 mM ammonium bicarbonate buffer and proteins were digested for 18 hours at 36°C using trypsin/LysC mixture (Promega) in 1:50 (w/w ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR mixture was at a volume of 10 μL containing 5 μL SYBR Premix Ex Taq (Promega, Madison, USA), 0.5 μM each of the primers ...
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bioRxiv - Cancer Biology 2020Quote: ... and cells were lysed in a mixture of 50 µL media and 50 µL CellTiter-Glo 2.0 reagent (Promega # G9243) per well ...
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bioRxiv - Genomics 2022Quote: ... samples were diluted 3 times with 50mM Tris buffer (down to 2M final urea concentration) and digested overnight at 37°C using a Trypsin/Lys-C mixture (Promega) at a ratio 1:100 w/w ...
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bioRxiv - Cancer Biology 2022Quote: ... 25 μL of each were then transferred to a fresh white 96-well plate containing 25 μL of GSH-Glo mixtures (Promega). Luminescence was determined using a Tecan Spark 10 microplate reader
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bioRxiv - Cell Biology 2019Quote: ... RT reaction was carried out in 15 ul reaction mixture at 40 °C for 60 min after addition of ImProm II (Promega). DNA fragments for L chain and the variable region of H chain (VH ...
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bioRxiv - Cell Biology 2022Quote: ... then excised and gel slices dehydrated with methanol (100mM) ammonium bicarbonate (100mM) mixture before digestion with 200ng of trypsin (Promega) overnight at room temperature ...
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bioRxiv - Cancer Biology 2019Quote: ... and cells were lysed in a mixture of 50 μL media and 50 μL CellTiter-Glo 2.0 reagent (Promega # G9243) per well ...
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bioRxiv - Molecular Biology 2020Quote: ... and protein complexes were directly digested on-beads by the addition of 2 μg of a Trypsin/Lys-C mixture (Promega). Peptides were isolated on C18 resin tips according to the manufacturer’s instructions (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... 5 µl of the cDNA reaction mixture was used as a template in a 50 µl PCR amplification reaction mixture with corresponding forward and reverse primers (Table S2) and GoTaq DNA polymerase (Promega), as described by the supplier ...
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bioRxiv - Plant Biology 2021Quote: ... reverse transcribed with oligo(dT)15 in a 20-μL reaction mixture using the Moloney murine leukemia virus reverse transcriptase (Promega) or the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific) ...
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bioRxiv - Immunology 2021Quote: ... cells were transfected with a DNA mixture containing 50 ng of firefly luciferase reporter plasmid (Promega pGL4.31 [luc2P/Gal4UAS/Hygro]), 5 ng of Renilla luciferase plasmid (Promega pRL-CMV) ...
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bioRxiv - Developmental Biology 2022Quote: ... in a 12 µl mixture consisting of 6 µl Blue SYBR Green mix (Biozym) or GOTaq qPCR Master Mix (Promega), 2 µl primer pairs (2.5 µM each ...
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bioRxiv - Developmental Biology 2023Quote: ... The synthesis of cDNA was achieved by incubation in a 20 µl reaction mixture containing 200 U of MMLV Reverse Transcriptase (Promega) and 500 ng of random primer (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... using 3 µl of the in vitro translation reaction mixture in 150 µl of luciferase assay reagent (Promega, Fitchburg, WI). The graphs in Fig ...
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bioRxiv - Molecular Biology 2023Quote: ... The media mixture was removed from each well and the cells were lysed with a room tempered 1xGlo buffer (Promega) for 5 min at 25°C while shaking at 550 rpm ...
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bioRxiv - Genomics 2023Quote: DNA was extracted from the agar/cell mixture from the output and control pools using a Wizard Genomic DNA Purification Kit (Promega). The manufacturer’s protocol was followed with two modifications ...
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bioRxiv - Cell Biology 2023Quote: ... China and listed in Table S2) were incubated with 201tμL of the real-time PCR reaction mixture including GoTaq qPCR Master Mix (Promega, USA) at 95°C for 3 min ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in 70 μL of a 50/50% mixture containing PBS and the Caspase-Glo 3/7 reagent (Promega). Lysates were incubated at least 2 hours protected from the light at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... The final 50 µL translation reaction included the 5 µL RNA mixture described above and components from the Rabbit Reticulocyte Lysate system (Promega): 35 µL of rabbit reticulocyte lysate ...
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bioRxiv - Biophysics 2024Quote: ... 8 μl of the terminated reaction mixture were then applied to a streptavidin biotin capture membrane (SAM2, Promega, Cat.# V2861) and further treated following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Homogenates were diluted 1/100 with the extraction buffer and mixed with the luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay, Promega). Luminescence was measured with a SpectraMax Gemini XPS luminometer (Molecular Devices) ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins were extracted from protoplasts after a 16h incubation in WI medium using 100 μl of 1 × passive lysis buffer (Promega). Luciferase activities were measured using the Dual Luciferase Reporter Assay kit (Promega ...
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bioRxiv - Microbiology 2020Quote: ... This was followed by in-gel digestion using 1:100 ratio enzyme:sample (w:w) using as enzyme sequencing grade modified trypsin (Promega, Madison, WI) overnight at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was eluted from the beads using the elution buffer (1% SDS and 100 mM NaHCO3) and purified by PCR purification kit (Promega). The final products were analyzed by quantitative real-time PCR with the primers listed below:
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bioRxiv - Evolutionary Biology 2022Quote: ... the proteins were alkylated with iodoacetamide (100 mM for 1 h at room temperature in a dark chamber) and digested with trypsin (Promega) diluted in ammonium bicarbonate (0.1 M for 24 h at 37 °C) ...
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bioRxiv - Cell Biology 2022Quote: ... and dried in at 37 °C before overnight digestion with 30 μL trypsin (1:100 g/g, sequencing grade modified trypsin V5111, Promega) at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... Nuclei pellets were re-suspended in 100 µl of PBS containing 1 U/μl RNasin® Ribonuclease Inhibitors (Promega, # N2615) with or without 5 µg/µl of yeast tRNA (Thermo ...
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bioRxiv - Systems Biology 2019Quote: ... and dried in an oven at 37 °C) before overnight digestion with 20 μL trypsin (1:100 g/g, sequencing grade modified trypsin V5111, Promega) at 37 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were harvested 36 h after transfection by lysing in 100 μl of 1× reporter lysis buffer (Promega, Madison, WI). Lysates were pelleted ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 50 μL (fLuc assays) or 100 μL (NanoLuc assays) of 1× Cell Culture Lysis Reagent (Luciferase Assay System; Promega) and incubated for 10 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed with PBS and the cells were lysed with 1× reporter lysis buffer (100 µl per well; Promega). Following a freeze-thaw cycle ...
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bioRxiv - Neuroscience 2021Quote: ... The transfected cells were then treated with Aβ42 (1-4 μM) or calcitriol (100 nM) for 6 h before luciferase activity assay (Dual-Luciferase Reporter Assay, Promega). A 587-bp region of the human Cyp24a1 gene promoter that contains two VDRE motifs was cloned into the promoter-less luciferase expression vector pGL3-basic (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... of 50mM Tris-HCl pH 8.5 containing LysC (Wako Chemicals) at 1:100 (w/w) ratio and trypsin (Promega V5113) at 1:100 (w/w ...
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bioRxiv - Cell Biology 2023Quote: ... diluted 1:100 in storage buffer and analyzed as indicated in the manufacturer’s protocol (LDH-Glo Cytotoxicity Assay, Promega, #J2381).
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bioRxiv - Cell Biology 2023Quote: ... Isolated proteins were digested with 1:50 w/w LysC (Wako Chemicals, cleaves at the carboxylic side of lysine residue) and 1:100 w/w trypsin (Promega, Sequencing-grade ...
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bioRxiv - Molecular Biology 2023Quote: ... The library preparation involved using 100 ng of genomic DNA spiked with 1% (w/w) of unmethylated lambda DNA from Promega. Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... For transient expression experiments each well was transfected with 100 ng of DNA using 1:3 ratio of FUGENE HD (#E2311, Promega). Twenty-four hours after transfection or doxycycline stimulation ...
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bioRxiv - Biochemistry 2023Quote: ... Samples containing 20-30 pmol rhodopsin were mixed with 100-250 fmol BSA and cleaved with 1 μg trypsin/LysC mix (Promega) using the SP3 beads protocol described in [29].