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Citations for Promega :
251 - 300 of 3328 citations for Polybrominated Diphenyl Ether Predominant Congener Mixture 1 100 Dilution Unlabeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then lysed in 100 μl of 1× Passive Lysis Buffer (Promega) and luciferase activity in 10 μl aliquots of the cell lysates was measured using the Dual Luciferase Reporter Assay System (Promega ...
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bioRxiv - Cancer Biology 2020Quote: ... in a ratio of 100:1 using ViaFect transfection reagent (Promega Cat# E4982). Seven TCF/LEF-binding sites are present upstream of a firefly luciferase gene in the TOPflash vector ...
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bioRxiv - Cell Biology 2021Quote: ... cells were again washed with DPBS and incubated with 90 μL of a 1/1000 dilution of furimazine stock solution (Promega) in 0.1 % BSA/HBSS ...
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bioRxiv - Genomics 2021Quote: ... Cell number was normalized before seeding by measuring ATP levels in a 1:2 dilution series of digested organoids with CellTiter-Glo (Promega). The number of cells matching 10,000 photons (Berthold Technologies ...
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bioRxiv - Microbiology 2020Quote: ... used at a 1:10,000 dilution and the secondary antibody was an Anti-Mouse IgG HRP conjugate antibody (Promega, #W4021) used at a 1:10,000 dilution.
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bioRxiv - Microbiology 2020Quote: ... the plates were washed with PBS-T five times and added with a 1:5000 dilution of HRP-conjugated goat anti-mouse IgG antibody (Promega) in blocking buffer at 37 °C for 45 min ...
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bioRxiv - Microbiology 2020Quote: ... The plates were washed with PBS-T 5 times and a 1:5000 dilution of HRP-conjugated goat anti-rabbit IgG antibody (Promega) was added for 45 min at 37 □ ...
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bioRxiv - Bioengineering 2021Quote: ... knees were injected with 50 μL furimazine (1:50 dilution in PBS, Nano-Glo™, PRN1120, Promega, Madison, WI, USA), then assessed with the above IVIS settings ...
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bioRxiv - Neuroscience 2023Quote: ... We performed targeted PCR by adding 1 μL of cell lysate (1:5 dilution) to a 25-μL PCR reaction containing GoTaq Hot Start Master Mix Green (Promega) and 0.5 μL of the primers (10 µM ...
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bioRxiv - Genomics 2022Quote: ... The samples were diluted 1:1 with milliQ water and were incubated with a 1:100 w/w amount of trypsin (Promega, sequencing grade) overnight at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... the samples were diluted to 1 M Urea with 10mM EPPS pH 8.2 and incubated overnight with 1:50 LysC (Wako Chemicals) and 1:100 Sequencing grade trypsin (Promega). Digests were acidified using TFA and tryptic peptides were purified by tC18 SepPak (50 mg ...
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bioRxiv - Cell Biology 2022Quote: ... polysome were stabilised by washing cells in 20 ml ice-cold water containing 0.1 mg/ml CHX before being resuspended in 1 ml RiboTag Lysis Buffer (50 mM Tris, pH 7.5, 100 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1 mM DTT, 100U/mL Promega RNasin ...
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bioRxiv - Cancer Biology 2021Quote: ... the samples were diluted to 1 M Urea with 10 mM EPPS pH 8.2 and incubated overnight with 1:50 LysC (Wako Chemicals) and 1:100 Sequencing grade trypsin (Promega). Digests were acidified using TFA and tryptic peptides were purified by Oasis Prime HLB columns (30 mg ...
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bioRxiv - Molecular Biology 2019Quote: ... Cell pellets were lysed in 300µl of Lysis Buffer (50mM Tris-HCl pH 7.5, 150mM NaCl, 1% Triton® X-100, 0.1% deoxycholate, Protease Inhibitor and RNasin Plus [1:500, Promega]),diluted to a final concentration of ~1µg/µl with 100mM Tris-HCl pH 7.5 ...
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bioRxiv - Immunology 2021Quote: ... The media from infected cells was then removed and 100 μl of 1:1 diluted PBS:Bio-Glo (Promega, G7940) luciferase substrate was added to each well ...
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bioRxiv - Cancer Biology 2020Quote: ... the samples were diluted to 1 M Urea with 10mM EPPS pH 8.2 and incubated overnight with 1:50 LysC (Wako Chemicals) and 1:100 Sequencing grade trypsin (Promega). Digests were acidified using TFA and tryptic peptideswere purified by tC18 SepPak (50 mg ...
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bioRxiv - Immunology 2023Quote: ... Media were removed and 100 μl of CTG reagent (CellTiter-Glo®, Promega GmbH, Germany; 1:1 with PBS) per well was added ...
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bioRxiv - Plant Biology 2023Quote: ... Sample C was treated with 1% Triton X-100 (TX100) followed by treatment with 1μg mL-1 trypsin (Promega). For the detergent treatment ...
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bioRxiv - Neuroscience 2023Quote: ... and Dounce homogenized in 500 µL of ice-cold RNase-free buffer polysome buffer (50 mM Tris pH 7.5, 100 mM KCl, 12 mM MgCl2, 1% NP-40, 1 mM DTT, 200U/ml Promega RNasin ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 30 μL/well of the CellTiter-Glo ATP content assay reagent mixture (Promega, #G7573) was added that was incubated for 10 min at room temperature followed by a detection of luminescence signal on a Perkin Elmer Envision or BMG CLARIOstar plate reader ...
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bioRxiv - Immunology 2022Quote: ... The mixture was measured for luciferase production using a luminescent substrate (Promega, Cat#E2510).
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bioRxiv - Microbiology 2019Quote: ... The reaction mixtures were then ligated into pGEM-T Easy vector (Promega Catlog#A1360) overnight at 4°C and then transformed into TOP10 competent E ...
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bioRxiv - Molecular Biology 2019Quote: ... Final reaction mixtures consisted of: 1x concentrated GoTaq® Green buffer (Promega, Madison, US); 0.7 units of GoTaq® DNA Polymerase (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... The mixture of NanoBRET™ Nano-Glo® substrate and Extracellular NanoLuc Inhibitor (Promega) was added immediately prior to BRET luminescence measurement (450 nm for donor emission and 610 nm for acceptor emission ...
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bioRxiv - Synthetic Biology 2020Quote: PUREfrex2.0 reaction mixtures were supplemented with 0.5 μL of GreenLys (FluoroTect™ GreenLys, Promega) and gene expression was performed in a test tube as described above ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Luminescence was initiated by addition of a mixture of 2 μM coelenterazine-h (Promega) and 40 μM 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Molecular Biology 2023Quote: ... The cold mixture was quickly transferred to a PCR-clean up column (Promega, A9282) and centrifuged for 30 s at 14,000g ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR reaction mixture for all reactions consisted of 2x GoTaq Green Mastermix (Promega), 400nM primer-F ...
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bioRxiv - Biochemistry 2023Quote: ... cDNA was synthesized from each reaction mixture using the GoScript Reverse Transcription System (Promega) per the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2020Quote: ... 150 mM NaCl, 1 mM MgCl2, 2 mM EDTA, 1% NP-40, 1 mM DTT, 100 U/mL RNasin [Promega], 1X protease inhibitor) at 7 dpi ...
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bioRxiv - Microbiology 2019Quote: ... followed by 350 μL RNA Dilution Buffer (Promega kit) were added to the cell suspensions ...
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bioRxiv - Systems Biology 2022Quote: ... and 20x stock in NanoBRET Tracer Dilution buffer (Promega) in a PP 384-well plate ...
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bioRxiv - Cell Biology 2021Quote: ... with the Nano-Glo LCS Dilution Buffer (Promega, 0000333050) to make the Nano-Glo Live Cell Reagent ...
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bioRxiv - Biochemistry 2023Quote: ... and a 100x dilution for RenillaLuc substrate (Promega, E28) using reaction buffer (20 mM Tris HCl ...
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bioRxiv - Cell Biology 2024Quote: ... at 5 µM in Tracer Dilution Buffer (Promega N291B) was added to all wells ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed thoroughly in PBS-T before incubation with secondary antibodies (anti-rabbit horseradish peroxidase, Promega WB401B 1:2,500 dilution) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... transfected cells grown for 48 hours in 96-well plates were washed with Hanks′ Balanced Salt solution (HBSS) and incubated with 1/1,000 dilution of furimazine stock solution (Promega, WI, USA). After incubation for 2 minutes ...
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bioRxiv - Microbiology 2023Quote: ... The culture was diluted 1:100 in fresh LB supplemented with chloramphenicol and kanamycin including a 1:5000 dilution of the Nano-Glo® Luciferase Assay Substrate (Promega). Preliminary growth curves in the presence of the substrate showed no impact on growth at this concentration ...
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The key features of SARS-CoV-2 leader and NSP1 required for viral escape of NSP1-mediated repressionbioRxiv - Molecular Biology 2021Quote: ... 100 ng of Trypsin Gold and 1 μg of Trypsin sequenced grade (both Promega) were added to BioID eluates and total cell lysates ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.5) containing 1% Tween20 and 100 U/ml RNasin Plus RNase Inhibitor (Promega). After centrifugation at 15,000 g for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... and the proteins were digested with 1/100 (w/w) trypsin (Promega, Cat # V5111) overnight at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... for 4 h at 37 °C and then with trypsin (Promega, 1:100, w:w) overnight at 37 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Trypsin digestion was carried out overnight at 37°C with 1:50-1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... Trypsin digestion was carried out overnight at 37 °C with 1:50 - 1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Immunology 2022Quote: ... plates were equilibrated at room temperature for at least 30 minutes before 100 µL of 1:1 diluted PBS and CellTiter Glo reagent (Promega) was added to 100 µL of media ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mM KCl, 5 mM MgCl2, 2 mM DTT, 100 μg ml-1 cycloheximide, and 20 U ml-1 RNase inhibitor [Promega].) Gradients were centrifuged 36,000 rpm for 2.5 h in a SW41 Ti rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2019Quote: ... the wells were washed and 200 µl of a 1:5,000 dilution of secondary Anti-Mouse IgG antibody conjugated to HRP (Promega Corporation, Madison, WI) in PBS + 2% BSA was added to each well ...
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bioRxiv - Bioengineering 2023Quote: Mice were administered either 200μL of 15 μg/mL D-luciferin or a 1:40 dilution of Nano-Glo substrate (Promega, diluted in DPBS) by intraperitoneal injection for firefly luciferase and Antares or nanoluciferase imaging ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... The gradients were centrifuged in an SW41 rotor at 200,000xg for 1hr Fractions were collected from air-gradient interfaces and complemented using LgBiT (Promega, 1:200 dilution) and nanoluc substrate (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... Gel pieces were rehydrated in a mixture of 4 ng/µL trypsin (Promega, Madison, WI) and 0.01% ProteaseMAX (Promega ...