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Citations for Promega :
351 - 400 of 3328 citations for Polybrominated Diphenyl Ether Predominant Congener Mixture 1 100 Dilution Unlabeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 100 nM streptavidin (Promega) was included in the master mix ...
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bioRxiv - Synthetic Biology 2024Quote: ... in live cell substrate (LCS) dilution buffer (Nano-Glo® assay, Promega) was added to each well ...
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bioRxiv - Immunology 2022Quote: ... and digested over-night with Lys-C-Trypsin mix (1:100 enzyme to protein ratio) and trypsin (Promega; 1:50 enzyme to protein ratio). Following the digestion step ...
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bioRxiv - Genomics 2020Quote: ... PCR amplification was performed in a 10- μL reaction mixture containing 5 μL of GoTaq Master Mix (Promega), 5 pmol FAM-labeled universal primer (5′ - FAM-gctacggactgacctcggac-3′) ...
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bioRxiv - Cell Biology 2021Quote: ... the reactions were carried out in triplicates in a reaction mixture containing 1x GoTaq qPCR MasterMix (Promega Corporation), 300 nM CXR Reference Dye ...
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bioRxiv - Zoology 2021Quote: ... Sections were digested in a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard DNA isolation kit) and 3% 20mg/ml Proteinase K (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... The reaction mixture (15 μl) contained about 7.5 μL of GoTaq® G2 Green Master Mix (Promega, USA), 1.0 μL of primer ...
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bioRxiv - Biochemistry 2022Quote: ... 25 μL of this mixture was mixed with 25 μL of Nano-Glo® Luciferase Assay Reagent (Promega), following manufacturer’s protocols ...
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bioRxiv - Neuroscience 2020Quote: ... was incubated for 20 min with a mixture of 57 µL OptiMEM and 3 µL FuGene6 (Promega E2692). After FuGene6/DNA complex formation ...
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bioRxiv - Molecular Biology 2019Quote: ... We pooled the BKE strains and prepared a genomic DNA mixture using Wizard genome DNA purification kit (Promega). We used this DNA preparation to transform an smpB-deleted strain of B ...
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bioRxiv - Microbiology 2020Quote: ... where the samples were destained and subjected to in-gel proteolytic digestion with trypsin/Lys-C mixture (Promega). The digests were extracted ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were transfected the next day with a mixture of TP1-firefly luciferase and pRL-TK (Promega) plasmids at a 49:1 ratio using Lipofectamine 2000 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... 100 µl of samples were mixed with 100 µl BacTiter-GloTM reagent (Promega) and incubated for 5 min in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... Each organ was homogenized with a lysis solution containing a 1:5 dilution of Luciferase Cell Culture Lysis 5X Reagent (Promega, Madison, WI, USA, cat. no. E1531) in Millipore water supplemented with 1 % (v/v ...
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bioRxiv - Microbiology 2020Quote: ... Samples were diluted with three volumes digestion buffer (100 mM Tris/HCl pH 8.5, 1 mM CaCl2) and 0.25 μg Trypsin (Sequencing Grade, Promega) were added and incubated o/n at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... and added to 50-100 µL ribosome-coupled anti-Flag M2 agarose beads and 1 µL RNasin (Promega) per reaction ...
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bioRxiv - Cancer Biology 2019Quote: ... ATP solution (5 μL of a 100 μM solution containing 0.1 μg 4:1 glycine:tyrosine peptide substrate (Promega)) was added and incubated at 37 °C for 20 min before the addition of ADP-Glo reagent (5μL ...
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bioRxiv - Plant Biology 2023Quote: ... Total proteome peptide pools were generated by sample digestion overnight with 1:100 sequencing grade trypsin (V5113; Promega). Generated peptide pools were quantified by Nanodrop ...
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bioRxiv - Plant Biology 2023Quote: The substrate of the nano-luciferase diluted at 1:100 (v/v) (furimazine, Kit from Promega (ref 1110)) was spread on seedling that grew on Agar plates ...
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bioRxiv - Microbiology 2024Quote: ... 50 to 100 ng of template were used per reaction with 1.5 µl rRNasin 40 U.ml-1 (Promega), 5 µl 5x optimized transcription buffer (Promega) ...
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bioRxiv - Molecular Biology 2019Quote: ... for 30 minutes at room temperature and then digested by LysC (Lysyl endopeptidase, #125-05061, Wako, ratio LysC/protein 1:100) and trypsin (Trypsin Gold, V5280, Promega, ratio trypsin/protein 1:20) overnight ...
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bioRxiv - Bioengineering 2024Quote: ... incubated in 200 µl of 40-fold dilution of Nano-Glo substrate (Promega) in PBS per lung at room temperature for 5 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... samples containing 100 μg total protein were mixed with 100 μl Luciferase Substrate (Promega). The kinetics of the luminescence was recorded for 10 min using a TriStar2 S LB 942 Plate Reader (Berthold Technologies ...
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bioRxiv - Biochemistry 2020Quote: ... PCR reaction mixture was purified with the Wizard® SV Gel PCR Clean-Up System (Promega Corporation, WI, USA). One Shot® TOP10 Chemically Competent Escherichia coli (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Each PCR reaction was performed in a 10 μL mixture containing 0.25 U GoTaq G2 Hot Start Polymerase (Promega), 1x GoTaq G2 Buffer ...
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bioRxiv - Zoology 2020Quote: ... Other sections were digested in a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard DNA isolation kit) and 3% 20mg/ml Proteinase K (Promega) ...
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bioRxiv - Genetics 2023Quote: ... proteins were extracted by chloroform-methanol precipitation and digested with 2 μg trypsin/Lys-C mixture (Promega, Madison, WI). Samples were labeled with 10-plex tandem mass tag reagents (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... the DNA-coated beads were added to the interaction mixture (BB 1x : 20mM HEPES pH7.9, 100mM KCl, 5mM, 10% Glycerol; supplemented with 160µg acetylated-BSA (Promega R396A), 1.44nmol ds oligoB ...
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bioRxiv - Cancer Biology 2024Quote: ... the kinase reaction mixtures were collected after incubation and added into equal volume Kinase-Glo® regent (V6711, Promega) for 10 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: DNA purification from PCR mixture was done using Wizard SV Gel and PCR Clean-Up System Kit from Promega as per manufacturer’s instructions.
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bioRxiv - Bioengineering 2020Quote: ... Trypsin gold (100 nM; Promega) was added to each sample and proteolysis proceeded for 2 h at 37 °C ...
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bioRxiv - Neuroscience 2019Quote: ... 100 U/ml RNasin (Promega) and protease inhibitor cocktail (Roche) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and trypsin (100 ng; Promega). We mixed the resulting peptides with a MALDI matrix consisting of an aqueous 50% acetonitrile/0.1% TFA solution of α-cyano-4-hydroxycinnamic acid (5 mg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... 100 ng Random Primers (Promega), 400 ng Anchored Oligo (dT)20 Primer (Invitrogen) ...
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bioRxiv - Physiology 2019Quote: ... 100 U/mL RNasin (Promega), 100 μg/mL cycloheximide ...
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bioRxiv - Cell Biology 2024Quote: ... and 100 μM luciferin (Promega). Chondrocytes were incubated in the presence of 100 nM of dexamethasone for 60-min prior to the media change ...
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bioRxiv - Cell Biology 2024Quote: ... and 100 μM luciferin (Promega). Samples were maintained in 35 mm dishes sealed with 40 mm glass cover slips (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 U/mL RNasin (Promega), complete protease inhibitor (Roche)] ...
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bioRxiv - Biochemistry 2021Quote: ... free thiols were alkylated by iodoacetamide and proteins were sequentially digested with endoproteinase Lys-C (Wako, 1:100 enzyme-to-substrate ratio, 3 h at 37 °C) and trypsin (Promega, 1:50, overnight at 37 °C). Digested samples were desalted ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.05% (v/v) NP-40 and 0.5% (v/v) Triton X-100) containing 20 U ml−1 of RNasin (Promega), 1 mM DTT ...
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bioRxiv - Molecular Biology 2020Quote: Gently re-suspend cells in 100 μl of 3% Glyoxal fixation solution with 1:25 RNasin Plus (Promega N261B) and incubate for 15 minutes on ice.
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bioRxiv - Developmental Biology 2020Quote: ... Postnatal testes chemically synchronized for undifferentiated spermatogonia were homogenized at 2% w/v in polysome buffer (50 mM Tris, pH 7.4, 100 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1mM DTT, 200 U/mL Promega RNasin ...
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bioRxiv - Microbiology 2021Quote: ... Protein was reduced with 5 mM DTT and alkylated with 14 mM iodoacetamide followed by digestion with trypsin (1:100, w:w) (Promega) as described in [80] ...
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bioRxiv - Cell Biology 2020Quote: ... at a protein:Lys-C ratio of 100:1 (w/w) for 4 h at 37°C followed by trypsin (Promega) digestion at a ratio of 50:1 (w/w ...
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bioRxiv - Cancer Biology 2020Quote: ... RT) and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:100, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:100 ...
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bioRxiv - Developmental Biology 2020Quote: ... Tryptic digests were performed overnight after addition of 46 μl of 100 mM Hepes pH 7.6 and 4 μl of 1 μg/μl Trypsin Gold (Promega).
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bioRxiv - Neuroscience 2022Quote: ... to a final urea concentration of 2 M for overnight Trypsin/Lys-C digestion at 35°C (1:100 protease:substrate ratio, Mass Spectrometry grade, Promega Corporation ...
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bioRxiv - Microbiology 2023Quote: ... At 8 and 24 hpt monolayers were lysed with 100 μl 1 x passive lysis buffer according to the manufacturer’s instructions (Promega), stored at −80 °C and analysed using Dual-luciferase substrate (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... 100 ng of purified genomic DNA was spiked with 1 ng of unmethylated lambda DNA (Promega, Madison, WI, USA) and subjected to bisulfite treatment using the EZ DNA methylation gold kit (Zymo Research ...
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bioRxiv - Cancer Biology 2023Quote: ... 170 µL medium was removed and 30 µL caspase 3/7 reaction (1:100 Z-DEVD-R110 fluorogenic substrate:Homogenous buffer; ApoONE, Promega) was added to each well ...