Labshake search
Citations for Promega :
551 - 600 of 4075 citations for NGS ChIP Seq kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Cells were then transfected with 1500 ng pcDNA-FLAG-HA-sscGAS plasmid complexed with FuGene 6 reagent (Promega) according to manufacturer’s instructions or treated with FuGene 6 alone as a negative control ...
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bioRxiv - Microbiology 2021Quote: ... and 800 ng pSPAX2 were co-transfected into HEK 293T cells using the FuGENE HD Transfection Reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were digested with shaking overnight at room temperature in 300 ng of sequencing grade modified trypsin (Promega). Samples were then acidified with trifluoroacetic acid (TFA ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were digested overnight at 37 °C with 6 ng μL-1 of Asp-N (Promega, UK). Peptides were extracted in 2 % v/v formic acid ...
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bioRxiv - Systems Biology 2022Quote: ... followed by 4-fold dilution and an overnight digestion with 500 ng trypsin (Promega, Charbonnieres les Bains, France). Peptide mixtures were then desalted on C18 spin-column and dried on Speed-Vacuum.
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bioRxiv - Molecular Biology 2019Quote: ... was added to 500 ng of the cDNA-fragments in T7 transcription reaction buffer (Promega, Cat no. P2075), and incubated for 2 hours at 37°C in the absence or presence of N6-methyladenosine (Jena Biosciences ...
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bioRxiv - Molecular Biology 2019Quote: 1875 ng of circular plasmids were incubated at 37°C with 50 U of T3 RNA Polymerase (Promega) in a transcription buffer (40 mM Tris-HCl pH 7.9 ...
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bioRxiv - Cancer Biology 2019Quote: ... Dilute proteins were digested overnight with 5 μl of 25 ng/μl trypsin gold (Promega, Madison, WI, USA) and desalted with a ZipTipC18 pipette tip (Millipore ...
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bioRxiv - Microbiology 2020Quote: The protein samples were digested with 500 ng of sequencing grade trypsin (V511, Promega Co., Madison, WI, USA) and incubated at 37 °C following the protocol described by Shevchenko et al.22 ...
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bioRxiv - Microbiology 2019Quote: ... We reverse-transfected cells in each well with 10 ng of pGL4.23-CMV/NP-NLuc or pNL1.1/NLuc (Promega) negative control and 100 ng of pGL4.23-CMV/NP-HaloTag or pcDNA3.3/eGFP negative control ...
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bioRxiv - Microbiology 2019Quote: ... and rehydrated with 50 mM ammonium bicarbonate solution containing 12.5 ng/µL of sequencing-grade modified trypsin (Promega) at 4 °C for 45 min ...
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bioRxiv - Biochemistry 2019Quote: ... 0.2 μg pLEXm plasmid encoding WT or mutant KAP1 and 0.4 ng plasmid encoding Renilla luciferase using 1.5 μl of FuGENE 6 (Promega). Luciferase activity was measured 48 h post-transfection in cell lysates with the Dual Luciferase assay kit (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... The volume of each binding reaction was 100µl in SEQRS buffer containing 200 ng yeast tRNA competitor and 0.1 units of RNase inhibitor (Promega). The samples were incubated for 30min at 22°C prior to magnetic capture of the protein-RNA complex ...
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bioRxiv - Bioengineering 2020Quote: ... PCR reactions were carried out with 100 ng genomic DNA or cDNA in the GoTaq Master Mix (Promega) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was generated from 20-100 ng of RNA using the GoTaq 2-step RT-qPCR System (Promega). qPCR was performed with SYBR Green on a StepOnePlus real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2020Quote: ... The dried gel pieces were re-swollen by incubation in 100 mM ammonium bicarbonate pH 8.0 supplemented with trypsin (12.5 ng/μL; Promega) for 1 h at 4 C and then incubated overnight at 37°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Dry gel pieces were digested with 200 μL of mass spectrometry-grade trypsin solution (4 ng/μL, Promega) in AMBIC and incubated at 37°C for 18 h ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were co-transfected with 50 ng of the Renilla luciferase transfection control plasmid pRL-CMV (Promega #E2261) and 1 μg of either the ERα reporter plasmid XETL (here referred to as ERE-Luc ...
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bioRxiv - Molecular Biology 2023Quote: ... Enzymatic digestion was performed at 37°C and overnight with 500 ng trypsin (Promega, Charbonnieres les Bains, France). Peptide mixtures were then desalted on C18 spin-column and dried in a Speed-Vacuum.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The following amounts of DNA were used per well: 60 ng of pGloSensor-22F cAMP plasmid (Promega, E2301), 100 ng of receptor or empty vector (control ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... PCR reactions were performed using 10 ng of cDNAs (RNA equivalent) and the GoTaq DNA polymerase (Promega, # M8295). For each gene ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were transfected with the following plasmids and amounts per well: 25 ng SRE-Luc reporter (E134A, Promega), 75 ng Gαi or Gαi QL in pcDNA3.1+ ...
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bioRxiv - Biochemistry 2023Quote: ... alkylated with 55 mM iodoacetamide for 20min at room temperature and digested using 13 ng/μl trypsin (Promega) overnight at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... Solvent was removed in a vacuum centrifuge and 100 µl 10 ng/µl sequencing grade Trypsin (Promega, US) in 10 % ACN ...
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bioRxiv - Microbiology 2024Quote: ... Cells were transfected with 500 ng of each plasmid using 0.4 µl of Fugene 6 according to the manufacturer’s instructions (Promega) for 40 hours ...
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bioRxiv - Microbiology 2024Quote: ... Cells were transfected with 500 ng of each plasmid using 1.5 µl of Fugene HD according to manufacturer’s instructions (Promega) for 40 hours ...
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bioRxiv - Microbiology 2024Quote: ... 50 to 100 ng of template were used per reaction with 1.5 µl rRNasin 40 U.ml-1 (Promega), 5 µl 5x optimized transcription buffer (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: Kit (Promega). For transposition with an additional plasmid pCas12k ...
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bioRxiv - Cancer Biology 2021Quote: ... The digested vector (200 ng) was ligated with 1 µL of the oligonucleotides (0.45nmol/μl) using T4 DNA ligase (Promega) for 3 hrs ...
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bioRxiv - Cell Biology 2020Quote: ... the gel pieces were covered with 200 μl of 20 ng/μl of sequence grade trypsin/Lys-C (Promega) in 50 mM ammonium bicarbonate pH8 buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Trypsin digestion was performed by addition of 30 µL of 10 ng/µL sequencing grade trypsin (Promega, Madison, WI) in AmBic followed by 30 µL of additional AmBic ...
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bioRxiv - Molecular Biology 2021Quote: ... Gel pieces were rehydrated in 50 mM ammonium bicarbonate solution containing 12.5 ng/µL modified sequencing-grade trypsin (Promega) at 4°C before incubation overnight at 37°C ...
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CASC3 promotes transcriptome-wide activation of nonsense-mediated decay by the exon junction complexbioRxiv - Molecular Biology 2020Quote: ... Quantitative real time PCR was performed with 16 ng of cDNA per reaction with GoTaq qPCR Master Mix (Promega) and the CFX96 Touch Real-Time PCR Detection System (Biorad) ...
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bioRxiv - Cancer Biology 2019Quote: ... Viability was assessed 72 hours after treatment with 100 ng/mL leptin using the CellTiter Glo (CTG) assay (Promega).
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bioRxiv - Physiology 2020Quote: ... cDNA was added as 100 ng/reaction to SYBR green based GoTaq® qPCR Master Mix (Promega, Wisconsin, USA). The primer sequence for CEBPa was F ...
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bioRxiv - Microbiology 2019Quote: ... Samples were then incubated overnight at 37 °C with 500 ng Sequencing Grade Modified Trypsin (Promega, Madison, WI, USA). To achieve the complete digestion of the peptides ...
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bioRxiv - Microbiology 2020Quote: ... Transfection was performed using 100 ng of expression plasmid using 0.3 μl of FuGENE HD Transfection Reagent (Promega E2311) in 20 μl of Opti-MEM medium (Life Technologies) ...
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bioRxiv - Biochemistry 2021Quote: ... The next day the sample was further diluted to 1 M urea and 750 ng Glu-C (Promega, V1651) were added and incubated for another 8 hours at 37°C.
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bioRxiv - Cancer Biology 2020Quote: ... proteins on the affinity pulldown beads were digested overnight at room temperature with 100 ng sequencing grade trypsin (Promega) and peptides analyzed by nano-liquid tandem MS as described in102 using an 75 μm × 150 mm analytical column (C18 ...
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bioRxiv - Plant Biology 2021Quote: ... 200 ng of total RNA were reverse transcribed using 500 ng of oligo(dT)15 and the ImProm-II Reverse Transcription System following the manufacturer’s instructions (Promega). Quantitative real-time PCR reaction was performed on a Light Cycler 2 (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... The gel pieces were dried in a vacuum centrifuge then rehydrated in 50 mM ABC containing 12.5 ng/μL trypsin (Promega), 5 mM calcium chloride ...
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bioRxiv - Cell Biology 2021Quote: ... then resuspended in 200 µl of 25 mM ABC containing 500 ng of sequencing grade trypsin (Promega, catalog # V5111) and incubated for 16 h at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Dried reduced/alkylated samples were then rehydrated with 20 ng/µl trypsin in 100 mM NH4HCO3 (Promega, Madison WI) on ice for 1 h ...
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bioRxiv - Microbiology 2020Quote: Approximately 105 ng of full-length PCR product was ligated into the pGEM-T Vector System (Promega, Madison, WI), at a 3:1 molar ratio of insert:vector and incubated overnight at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Liquid was removed and gel pieces were dehydrated in 100% ACN at RT for 5 min at 2,000 rpm followed by rehydration in 100 mM NH4HCO3 containing 14 ng/μL trypsin (Promega) on ice for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant without beads was transferred to a new tube and digested with 150 ng trypsin (Trypsin Gold, Promega) in 1.5 µL 50 mM ammonium bicarbonate at 37°C for 5 hours ...
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bioRxiv - Cell Biology 2022Quote: ... 300 ng of genomic DNA was mixed with 0.2 μl of Go Taq®G2 flexi DNA polymerase (PROMEGA), 5.0 μl of 5X Green Go Taq ® Flexi buffer (PROMEGA) ...
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bioRxiv - Developmental Biology 2020Quote: ... with 100 ng of firefly luciferase reporter p12xAT_luc or p12xAT_luc(Mut) (Hojo et al., 2016) 15 ng of the pRL-TK Renilla luciferase control plasmid (Promega) and 100ng (unless otherwise indicated ...
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bioRxiv - Neuroscience 2021Quote: ... 100 ng of starting RNA equivalent cDNA was subjected to PCR reaction with the GoTaq green master mix (Promega) and 30 amplification cycles (94°C for 30 s ...