Labshake search
Citations for Promega :
451 - 500 of 4075 citations for NGS ChIP Seq kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... The proteins were digested overnight at 37 °C using sequencing grade trypsin (100 ng; Promega) in a buffer containing 25 mM 4-ethylmorpholine acetate and 5% MeCN ...
-
bioRxiv - Molecular Biology 2020Quote: ... a total of 200 ng plasmid DNAs including (1) pGL4.53 vector encoding Fluc gene (Promega), (2 ...
-
bioRxiv - Cell Biology 2021Quote: ... Proteins on the beads were digested with 200 ng trypsin/Lys-C mix (Promega, V5072) for 16 h at 37°C ...
-
bioRxiv - Microbiology 2022Quote: ... Neutravidin-bound proteins were digested on bead with 400 ng Trypsin/Lys-C mix (Promega) at 37 °C overnight with shaking ...
-
bioRxiv - Immunology 2022Quote: ... Proteolytic digestion was initiated by the addition of 250 ng of endoproteinase Lys-C (Promega) and incubating at room temperature for 2 hours ...
-
bioRxiv - Plant Biology 2022Quote: ... Proteins were digested overnight at 37°C using 500 ng of trypsin (Trypsin Gold, Promega). Supernatants were kept and peptides remaining in gel pieces were further extracted with 1% (v/v ...
-
bioRxiv - Molecular Biology 2019Quote: ... The protein samples were digested with 10 ng/μL Sequencing Grade Modified Trypsin (V5111, Promega) overnight ...
-
bioRxiv - Developmental Biology 2021Quote: ... a second overnight digestion step using 200 ng modified porcine trypsin (Promega, Madison, WI, USA) was performed at 37°C ...
-
bioRxiv - Immunology 2022Quote: ... 100 ng of RNA was reversed transcribed using M-MLV Reverse Transcriptase (Promega, Mannheim, Germany) with dNTPs (Bio-Budget ...
-
bioRxiv - Cell Biology 2022Quote: ... and then digested overnight with 300 ng of Trypsin/Lys-C Mix (Promega, Madison, WI). For on-bead digestion following SA pull-down ...
-
bioRxiv - Cancer Biology 2023Quote: ... Beads were resuspended in 50 mM ammonium bicarbonate containing 25 ng of Trypsin (V5111, Promega) and digested overnight at 37 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... we added 500 ng of trypsin/Lys-C Mix (CAT# V5072, Promega, Madison, WI, USA) to the beads soaked in 50 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Bioengineering 2023Quote: ... 2.25 ng/uL Single-Stranded DNA Binding Protein (Sigma, cat# S3917 OR Promega, cat# M301A). 12.5 uL of master mix was mixed with 12.5 uL of 2x input oligonucleotide ...
-
bioRxiv - Molecular Biology 2023Quote: ... and resuspended in 40 μl 2.5 ng/μl trypsin (V5111 sequencing grade modified trypsin, Promega) for overnight digestion at 37 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... followed by 500 ng of trypsin/Lys-C Mix (CAT# V5072, Promega, Madison, WI, USA) and mixed gently at 37°C overnight to digest proteins ...
-
bioRxiv - Microbiology 2024Quote: ... amoA gene amplicons were generated using 0.6 ng DNA template with GoTaq DNA polymerase (Promega) and 0.5 μM primers (CamoA_19F [5’-ATGGTCTGGYTWAGACG-3’] and TamoA_632R-4 [5’-GCKGCCATCCATCKRTANGTCCA-3’] ...
-
bioRxiv - Cell Biology 2023Quote: ... Protein samples (about 20 μg of protein) were digested with 500 ng of trypsin (Promega) and peptides were analysed by an Ekspert nanoLC 42 nanoflow system (Eksigent Technologies ...
-
bioRxiv - Plant Biology 2023Quote: ... before 10 µl sequencing grade modified Trypsin (100 ng/µl) from Promega (Madison, WI, USA) dissolved in 100 mM ABC was added ...
-
bioRxiv - Neuroscience 2023Quote: ... 200 ng of RNA was used for cDNA synthesis following Goscript Reverse Transcriptase (Promega #237815) protocol ...
-
bioRxiv - Systems Biology 2023Quote: ... 1 µL of enzyme mix (lysC & trypsin, 10 ng/µL in water, Promega, Cat. V5072) was dispensed into each well by using the pL-volume dispensing function of the cellenONE at 20°C and 85% humidity ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.1% Triton X-100) with the addition 15 ng of Lambda DNA (Promega, Fitchburg, WI) in 20 µL total reaction volume for 3 h at 37 °C ...
-
bioRxiv - Microbiology 2020Quote: ... Reduced and alkylated proteins were in-gel digested with 100 ng trypsin (modified sequencing grade, Promega) overnight at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: ... cells were transfected with pAV-Tornado-RhoBAST plasmid (~200 ng/well) using FuGeneHD transfection reagent (Promega) according to the manufacturer’s protocol ...
-
MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... Sigma-Aldrich)–coated dishes or glass coverslips with 30 ng/ml of 2.5 S mouse NGF (G5141, Promega) in the Neurobasal medium containing B27 supplement (17504044 ...
-
bioRxiv - Molecular Biology 2020Quote: Samples were then incubated overnight at 37°C with 12.5 ng/μL trypsin (sequencing grade; Promega) in 25mM NH4HCO3 ...
-
bioRxiv - Cell Biology 2022Quote: ... cDNA was synthesized in duplicates from 100 ng total RNA using ImProm II reverse transcriptase (Promega), RNAsin (Promega ...
-
bioRxiv - Genomics 2020Quote: 20 ng of the library was amplified in triplicates using GoTaq flexi DNA polymerase (M7806, Promega) for 25 cycles with three pairs of FAS_i5_BR_F and FAS_i6_BR_R intronic primers (Baeza-Centurion et al. ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were either treated with 50ng/ml rat Nerve Growth Factor (NG; Promega, Madison, WI, USA) at 0 ...
-
bioRxiv - Molecular Biology 2019Quote: ... and 100 ng of total RNA was reverse transcribed into cDNA using Reverse Transcriptase System (Promega). Two μL of 1/5 diluted cDNA was used as a template for the real-time PCR amplification using PowerUp SYBR® Green Master Mix and a StepOne Plus system (Applied Biosystem ...
-
bioRxiv - Genomics 2019Quote: ... 2 μg of each of these reporter plasmids along with 100 ng of pRL-TK (Promega), a Renilla luciferase expression plasmid to control for transfection efficiency ...
-
Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Beads were then resuspended in 50 µL trypsinization solution consisting of 10 ng/µL Trypsin (Promega) in 100 mM tetramethylammonium bicarbonate (TEAB) ...
-
bioRxiv - Cell Biology 2021Quote: ... Gel pieces were rehydrated in 25 mM ammonium bicarbonate containing 200 ng of trypsin (V5113, Promega) and the samples were digested for 14 hours at 37 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... dried gel pieces were rehydrated in 50µl of 12 ng/µl LysC/Trypsin (Promega, Madison, WI), 0.01% ProteaseMAX surfactant (Promega ...
-
bioRxiv - Cell Biology 2022Quote: ... and pCMV-hyPBase was performed using 500 ng each DNA and 6 μl of FugeneHD (Promega) as per manufacturer’s instructions20,49,50 ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... S-Traps were loaded with 125 μL trypsin solution (50 ng/μL in 50mM TEAB; Promega) and incubated at 47°C for 2 hours ...
-
bioRxiv - Biophysics 2022Quote: ... Cells were transiently transfected with 50 ng each of the appropriate plasmids using FuGENE HD (Promega) at a FuGENE DNA ratio of 3:1 ...
-
bioRxiv - Neuroscience 2021Quote: ... In-gel digestion was performed using 5 ng/μL mass spectrometry-grade trypsin (Trypsin Gold, Promega) in 50 mM NH4HCO3 digestion buffer ...
-
bioRxiv - Cancer Biology 2021Quote: ... and 100-200 ng of cDNA was synthesized with random primers and reverse transcriptase (Promega, GoScript). Quantitative PCR was performed using 2.5 ng of cDNA in duplicates ...
-
bioRxiv - Physiology 2019Quote: ... USA) and digestion with 150 ng trypsin (5 h, 37 °C Promega, Sequencing Grade, Wisconsin, USA) using a Tecan Freedom EVO robotic liquid handler (Tecan Systems ...
-
bioRxiv - Cell Biology 2020Quote: ... 1000 ng of donor and gRNA plasmids in 1:1 ratio was used using ViaFect (Promega) transfection reagent as per manufacturer’s guidelines ...
-
bioRxiv - Cell Biology 2021Quote: ... 400 ng of RNA was treated with 1 unit of RQ1 RNase-Free DNase (Promega, M610A) at 37°C for 30 minutes and was inactivated by RQ1 DNase Stop Solution at 65°C for 10 minutes ...
-
bioRxiv - Immunology 2020Quote: ... Complementary DNA (cDNA) was synthesized from total RNA (500 ng) using M-MLV Reverse Transcriptase (Promega) as per the supplier’s protocol (1 hr ...
-
bioRxiv - Genomics 2021Quote: ... 50 ng of ligated product was transformed into 25 μl of E.coli competent cells (Promega, JM109), recovered in 250 μl of SOC for 1 hour at 37 °C following heat shock ...
-
bioRxiv - Cell Biology 2020Quote: ... They were transfected with multiple plasmids at an amount of 50 ng each using FugeneHD (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... a total of 75 ng cDNA in 20μl was synthesized by GoScript Reverse Transcriptase (Promega, A5000).
-
bioRxiv - Molecular Biology 2023Quote: 500 ng of total RNA were converted to cDNA using M-MLV Reverse Transcriptase (Promega, M1705) and Oligo(dt ...
-
bioRxiv - Plant Biology 2022Quote: ... 500 ng of total RNAs were treated with 1 unit of RQ1 RNase-Free DNase (Promega) for 30 min at 37 °C ...
-
Phosphoproteomics of cellular mechanosensing reveals NFATC4 as a regulator of myofibroblast activitybioRxiv - Systems Biology 2023Quote: ... cDNA was synthesized from total RNA (100 ng) using GoScript™ Reverse Transcriptase System (Promega, #A5000), and the reverse transcription was 42°C for 1h ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Cells were transfected with 500 ng DNA at approximately 70% confluence using ViaFect transfection reagent (Promega) and assayed 24 hours after transfection ...
-
bioRxiv - Neuroscience 2023Quote: ... The samples were then incubated overnight at 37°C with 100 ng trypsin (sequence grade, Promega). The peptides formed from the digestion were further purified by µC18 ZipTips (Millipore ...