Labshake search
Citations for Promega :
601 - 650 of 935 citations for Mouse Anti Human IgG Fc Alexa Fluor 405 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2024Quote: ... Proliferation rate of NIH-3T3 fibroblasts was determined 24 h after MNP treatment via MultiTox-Fluor Multiplex Cytotoxicity Assay (Promega). As controls ...
-
bioRxiv - Cell Biology 2024Quote: Cells were reactivated or mock treated for 48 h and then labelled at least 30 minutes prior to harvesting with Halo ligand Janelia Fluor 646 (Promega) at a 1:1,000 dilution of 20 μM stock in DMSO ...
-
bioRxiv - Immunology 2024Quote: ... 48 hours later the media was removed and replaced with 25 µL of RMPI-1640 media supplemented with 2% low-IgG FBS (Promega). Antibodies were serially diluted 1:10 in RPMI 1640 media from a starting concentration of 30 µg/mL to a final concentration of 0.014 µg/mL and 25 µL of antibody dilution was added to the plate ...
-
bioRxiv - Neuroscience 2021Quote: ... JF-549 or Halo-tag antibody (1:500, Promega, mouse monoclonal) and measuring the newly synthesized actin (in most cases ...
-
bioRxiv - Biochemistry 2022Quote: ... The SNRPN promoter was amplified from mouse genomic DNA (Promega, G3091) using design primers (Supplementary Table 3) ...
-
bioRxiv - Developmental Biology 2022Quote: ... from mouse genomic DNA and PCR products cloned into pGL4.23 (Promega) pre-digested with XhoI and HindIII using Cold Fusion Cloning kit (System Biosciences) ...
-
bioRxiv - Neuroscience 2023Quote: ... coated dishes with 30 ng/ml of mouse NGF (G5141, Promega). Then the neurons were microinjected with METTL3/14 activators (200 nl ...
-
bioRxiv - Microbiology 2024Quote: ... Mouse antibodies (CV801, CV804, CV820, CV925, CV1117) and effector cells (Promega) expressing mouse FcγRIV and NFAT luciferase reporter were added to the cells ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with a control Flag or HA-GSK-3βWT human plasmid using Fugene 6 (Promega #E2693) in a 2:1 ratio and incubated for 3 hours in a CO2 incubator ...
-
bioRxiv - Cancer Biology 2020Quote: ... T47D cells and MCF7 cells were transfected with empty or human LIP-containing pcDNA3.1 via Fugene HD (Promega) using the manufactures protocol ...
-
bioRxiv - Cancer Biology 2021Quote: The upstream 376 bp region of the human LGALS1 transcriptional start site was cloned into the pGL4.23 (Promega) vector to generate the LGALS1 luciferase reporter gene (LGALS1 pGL4.23 ...
-
bioRxiv - Cancer Biology 2022Quote: ... and MV4-11 (DSMZ Cat# ACC-102, RRID:CVCL_0064) human AML cell lines were identified by PCR-single-locus-technology (Promega, PowerPlex21 PCR Kit ...
-
bioRxiv - Biophysics 2019Quote: The cell-viability of human neuroblastoma (SH-SY5Y) cells was measured using MTT cell proliferation assay (Promega, G4000). SH-SY5Y cells were plated in a 96-well plate followed by differentiation in Neurobasal-A ...
-
bioRxiv - Molecular Biology 2019Quote: Full length 3’-UTR of human (P)RR and LDLR were cloned into the luciferase reporter vector (Promega). Mutant 3’-UTR luciferase reporter vectors were generated by PCR using site-directed mutagenesis technique ...
-
bioRxiv - Biophysics 2021Quote: ... Human Gβ with a C-terminal 15-amino-acid polypeptide linker followed by a HiBiT (peptide 86, Promega) and human Gγ were cloned into pFastBac vector ...
-
bioRxiv - Neuroscience 2024Quote: ... human CaV1.3 cDNA (accession number NM_001128840.2) was de-novo synthesized and assembled into a HaloTag vector (Promega G7721) using restriction cloning ...
-
bioRxiv - Microbiology 2023Quote: ... Standard curves were generated using purified genomic stocks (HSV-1 bacterial artificial chromosome and human genome Promega #G1471). Absolute copy number of genomic stocks was determined using droplet digital PCR (Biorad QX600) ...
-
bioRxiv - Cell Biology 2021Quote: ... anti-ARC (SantaCruz Biotechnology, #sc-17839, anti-HaloTag (Promega, #G9211), anti-SNAP-tag (New England Biolabs ...
-
Caspase-8 Modulates Angiogenesis By Regulating A Cell Death Independent Pathway In Endothelial CellsbioRxiv - Developmental Biology 2019Quote: The Caspase-8 Glo® assay was performed in combination with the CellTiter-Fluor™ Cell Viability Assay (both from Promega) according to the manual ...
-
bioRxiv - Cell Biology 2021Quote: ... conditions with HaloTag fusion protein expression were also incubated with 100nM of Janelia Fluor® 646 HaloTag® Ligand (Promega GA11220) for 30min prior to fixation ...
-
bioRxiv - Cell Biology 2019Quote: ... non-fluorescent blocking ligand was washed out and cells were additionally washed three times with fresh DMEM medium and incubated DMEM medium containing Janelia Fluor 646 (JF646) HaloTag ligand (Promega, GA1121) in final labeling concentration 200 nM for indicated timepoints.
-
bioRxiv - Cancer Biology 2021Quote: ... The viability of the cultures was determined 24 h and 48 h after EV treatments by using the CellTiter-Fluor cell viability assay kit from Promega (G6080) following manufacturers instructions ...
-
bioRxiv - Cell Biology 2022Quote: For STED imaging proteins of interest were genetically fused to the HaloTag (Halo) and subsequently labelled with Janelia Fluor®646 HaloTag ligand (#GA1121, Promega) by incubating 200 nM ligand (from 200 μM stock in DMSO ...
-
bioRxiv - Cell Biology 2023Quote: Cells grown under confluency were incubated in CM+bFGF media containing 1μM cell permeable Janelia Fluor® 549 HaloTag ligand complex (Promega, GA1110) for 1 hour at 37°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... A volume of cells equivalent to 1 mL at OD600 = 0.2 was centrifuged and re-suspended in 1ml fresh medium supplemented with JF549 (Janelia Fluor®HaloTag®Ligands, Promega) at a final concentration of 1 µM ...
-
bioRxiv - Biophysics 2023Quote: ... each respective cell type was incubated at 37 °C with 500 pM of Janelia Fluor® 646 HaloTag Ligand (NSCs, ECs, or keratinocytes) (Cat. No. GA1120, Promega) or Janelia Fluor® 635 HaloTag Ligand (MNRs ...
-
bioRxiv - Microbiology 2021Quote: ... infected target cells were washed with DPBS and the medium was replaced with 50 μl of RPMI 1640 with 4% low IgG serum (Promega # G711A) containing 5 x 104 Jurkat effector cells (Promega # G701A ...
-
bioRxiv - Microbiology 2023Quote: ... infected target cells were washed with DPBS and the medium was replaced with 40 μl of RPMI 1640 with 4% low IgG serum (Promega # G711A) containing 5 x 104 Jurkat effector cells (Promega # G701A ...
-
bioRxiv - Cancer Biology 2019Quote: ... rat or cynomolgus ICOS as target cells were co-incubated with ADCC reporter cells expressing human FcγRIIIa (V158; Promega) at a 5:1 ratio ...
-
bioRxiv - Biochemistry 2020Quote: ... Human β2AR was fused at its N-terminus to the ACP- or Halo7-tag protein (NEB and Promega, respectively) at the cDNA level ...
-
bioRxiv - Genomics 2022Quote: ADGRG6_3 and ADGRG_4 sequences were PCR amplified from human genomic DNA and cloned into the pGL4.23 plasmid (Promega, E8411). Human preadipocytes ...
-
bioRxiv - Molecular Biology 2020Quote: ... The promoter area of human Rpl28 gene was cloned at HindIII/NcoI site of the pGL3-basic (Promega, E1751) to make pGL3-reporters ...
-
bioRxiv - Genomics 2022Quote: Approximately 500-bp regions of DNA containing rs80282103 and rs11154336 were amplified from purified human genomic DNA (Promega, #G1521) by PCR using engineered restriction sites to allow directional cloning into the multiple cloning region of the pGL4.23[luc2/minP] luciferase reporter vector (Promega ...
-
bioRxiv - Neuroscience 2021Quote: We constructed luciferase reporter plasmids by cloning an ∼900 bp region containing human 1b30 into the pGL4.24 vector (Promega) upstream of the minP ...
-
bioRxiv - Biophysics 2022Quote: ... pHalo-SMARCA4 expresses human SMARCA4 with HaloTag fused to the N-terminus under a CMVd1 promoter (Promega ORF FHC12075). pHalo-MED26 expresses human MED26 fused with a HaloTag at the N-terminus and was a kind gift from Joan Conaway’s lab ...
-
bioRxiv - Cancer Biology 2022Quote: ... a 3kb region of the promoter containing HIC1 binding sites was amplified by PCR from human genomic DNA and cloned into the pGl4.26-luc vector (Promega); primers are listed in supplementary Table S1 ...
-
bioRxiv - Molecular Biology 2022Quote: ... The human Gβ1 with a C-terminal 15-amino acid polypeptide linker was followed by a HiBiT (peptide 86, Promega), and the scFv16 was modified with an N-terminal GP67 signaling peptide and a C-terminal 8× histidine tag ...
-
bioRxiv - Microbiology 2023Quote: ... and Sp-EVs towards human endothelial cells was accessed by the CellTiter-Blue® (CTB) Cell Viability Assay (Promega), according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... After 30 minutes of incubation at 37°C in 5% CO2 we added 50,000 ADCC-RL cells (Jurkat cell line expressing luciferase gene under the control of the NFAT response element and stably expressing human FcγRIIIa V158; Promega) in 50 µl RPMI 1640 medium with 6% low-IgG serum (Promega) ...
-
bioRxiv - Neuroscience 2023Quote: ... We amplified the regions from C57Bl/6 tail snip DNA or from human male genomic DNA (Promega catalog # G1471) using FastPhusion 2x Master Mix (Thermo Fisher catalog # F548L) ...
-
bioRxiv - Cell Biology 2023Quote: ... TTBK2 KO cell lines were seeded on glass coverslips (Matrigel-coated for hPSCs) and the next day transfected with 0.5μg TTBK1-HaloTag® human ORF in pFN21A (FHC12512, Promega) or pglap1-TTBK2 (“GFP-TTBK2” ...
-
bioRxiv - Genomics 2023Quote: ... Reference allele enhancer sequences for hZRS and hs737 were obtained via PCR cloning from human genomic DNA (Promega, G304A). Primers used for each enhancer sequence are outlined in Table S2 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... cell surface levels of HiBiT-tagged human S1PR1 were monitored using a Nano Glo HiBiT Extracellular Detection System (Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: IDH1 and NNT promoter sequences were amplified from genomic DNA of human primary melanocytes by PCR and cloned into pGL4.12 (Promega). Mutagenesis of E-boxes was performed using a QuikChange Site-Directed Mutagenesis Kit (Stratagene) ...
-
bioRxiv - Molecular Biology 2024Quote: ... and the equivalent region in the human (−207/+5) were cloned into pGL3-basic Luciferase reporter-vector (E1751, Promega) using the forward primer containing XhoI restriction site ...
-
bioRxiv - Developmental Biology 2021Quote: ... The primary antibodies used are mouse β-Gal (1:250; Promega #Z3781), mouse anti-Wingless (1:100 ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... or HA-mouse μOR and pGloSensor22F-cAMP plasmids (Promega, Madison, WI, USA) using Xtremegene9 (Sigma) ...
-
bioRxiv - Immunology 2021Quote: ... DNA was extracted from mouse tail using genomic DNA extraction kit (Promega Wizard SV Genomic DNA Purification System ...
-
bioRxiv - Molecular Biology 2022Quote: ... Both mouse brain and cell lysates were digested with DNase I (Promega) for 5 minutes at 37°C and then with RNase A (ThermoFisher ...
-
bioRxiv - Cell Biology 2022Quote: ... Goat immunoglobulins against rabbit or mouse immunoglobulins conjugated with horseradish peroxidase (Promega) were used as secondary antibodies ...