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Citations for Promega :
551 - 600 of 935 citations for Mouse Anti Human IgG Fc Alexa Fluor 405 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: The open reading frame for human SOX17 was PCR amplified using GoTaq Master Mix (Promega) from the PB-TRE3G-SOX17 plasmid (Table S5) ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5ng ml-1 recombinant human fibroblast growth factor (rhFGF) (Promega, Cat#G5071, Madison, WI, USA), and 1% chicken embryonic extract (Seralab ...
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bioRxiv - Genetics 2022Quote: ... human POPDC1 and POPDC2 cDNA sequences were cloned into the pFC14K or pFC32K plasmids (Promega), which contain C-terminus sequences for HaloTag and NanoLuc tags ...
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bioRxiv - Cell Biology 2021Quote: ... HaloTag TMR (G8251) and Janelia Fluor 646 HaloTag Ligand (GA1120) were obtained from Promega (Madison, WI, USA). SNAP-Cell 505-Star (S9103S ...
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bioRxiv - Cell Biology 2023Quote: ... Cells expressing HaloTag constructs were incubated overnight with 10-100 nM Janelia Fluor 646 HaloTag ligand (Promega) (61) ...
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bioRxiv - Cell Biology 2023Quote: The following dyes were obtained from these vendors: Janelia Fluor® HaloTag® Ligand 646 (GA112A; Promega), DAPI (D21490 ...
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bioRxiv - Genomics 2023Quote: ... and stained for 1hr at room temperature with the permeable Janelia Fluor HaloTag Ligand (JFX549; Promega #GA1110).
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bioRxiv - Cell Biology 2023Quote: ... plated in a 24-well glass-bottom plate were incubated with Janelia Fluor 646 HaloTag Ligand (Promega) for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were labelled at least 30 minutes prior to imaging with Halo ligand Janelia Fluor 646 (Promega) at a 1:1,000 dilution of 20 μM stock in DMSO and 10 uM Hoesct dye ...
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bioRxiv - Neuroscience 2020Quote: ... US) and TubβIII (mouse, 1:1000, G712A, Promega, UK). Slides were then incubated with the appropriate fluorophore-conjugated secondary antibodies ...
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bioRxiv - Biochemistry 2024Quote: ... 7C (mouse, Promega cat# N700A; 1:500-1:1000); Nluc in Fig ...
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bioRxiv - Cell Biology 2021Quote: ... Halo-tagged proteins were labeled by incubating cells with 200nM HaloTag Ligand conjugated to Janelia Fluor 646 (Promega) for 30 min before imaging ...
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bioRxiv - Biophysics 2022Quote: ... HaloTag substrates conjugated to Janelia Fluor® dyes ranging from 525 to 650 nm are available from Promega. Fig ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated with 0.7 nM Janelia fluor 646 dye conjugated to Halo-tag ligand (JF646; Promega) in the dark at 30°C for 15 min and washed with plain PYE to remove excess dye ...
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bioRxiv - Neuroscience 2024Quote: ... cells plated on glass-bottomed dishes were treated with Janelia Fluor 646 HaloTag Ligand (Promega, Cat. No. GA1120) at a final labelling concentration of 200nM (in cell culture media) ...
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bioRxiv - Neuroscience 2023Quote: ... Syp-Halo protein was incubated with 5 times molar excess halo ligand Alexa 488 (Promega, USA) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: Human Genomic DNA was extracted from the blood using the Wizard Genomic DNA Purification kit (Promega) as per the instructions.
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bioRxiv - Immunology 2021Quote: ... 25 μl of 7.5×104 Jurkat effector cells expressing either human FcγRIIIa or murine FcγRIV (Promega) resuspended in assay buffer were added to each well ...
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bioRxiv - Molecular Biology 2021Quote: Full-length human RNU6 sequence (110 nucleotides) was amplified by PCR and cloned into psiCHECK2 (Promega), downstream of Renilla luciferase ...
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bioRxiv - Neuroscience 2021Quote: Genomic DNA (gDNA) was extracted from human frontal cortex using Wizard Genomic DNA Purification Kit (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Standards for quantification were created by serial dilution of pre-quantified BSC human male DNA (Promega). Amplification reactions were performed in duplicate using 96 well-plates using a CFX96 Touch Real-time PCR Detection System (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: The region of interest (750-850 bp) was PCR amplified from pooled male human DNA (Promega) and cloned into a STARRseq luciferase validation vector_ORI_empty plasmid (Addgene plasmid #99298 ...
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bioRxiv - Molecular Biology 2020Quote: ... WT or mutant promoter of human Rpl28 gene was cloned into pGL-3-basic (Promega, E1751). The number is relative to transcriptional start site ...
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bioRxiv - Cancer Biology 2023Quote: ... Human and murine NLRC5 and CXCL10 promoters were cloned into pGL3 basic luciferase reporter vector (Promega). All mutated plasmids were constructed from wild type plasmids by Fast Mutagenesis System (TransGen Biotech) ...
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bioRxiv - Cell Biology 2023Quote: ... 10mM Ultrapure ATP and 0.7 μg of recombinant full-length human CDC7/DBF4 kinase (Promega, V5088). Reactions were incubated at 37 °C for 120 min and then terminated by freezing on dry ice before mass spectrometry.
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bioRxiv - Immunology 2024Quote: ... 3×106 ADCC and ADCP Bioassay Effector Cells expressing the human FcγRIIIa or FcγRIIa receptors (Promega) were added per well and the cells were incubated in at 37°C with 5% CO2 for 6 hours ...
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bioRxiv - Biochemistry 2019Quote: ... Approximately 15 μg of isolated IgG was treated with 0.1 μg of sequencing grade trypsin (Promega, Fitchburg, WI) and incubated overnight at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... following intraperitoneal injection of luciferin substrate (3mg/mouse; P1043 Promega). Leukemia burden in the peripheral blood was monitored twice weekly by flow cytometric analysis of hCD45+ proportions ...
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bioRxiv - Neuroscience 2021Quote: ... elegans and mouse cDNA (PolyATtract® mRNA Isolation Systems, Promega and ProtoScript® First Strand cDNA Synthesis Kit ...
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bioRxiv - Cancer Biology 2019Quote: ... Secondary antibodies where HRP conjugated (Promega, Rabbit W401B, Mouse W402B) for ECL detection using Pierce ECL2 solution (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... and HaloTag (mouse monoclonal, Promega no. G9211; 1:1000 dilution). Membranes were incubated for one hour at room temperature with IRDye secondary antibodies (Goat anti-Mouse 680RD ...
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bioRxiv - Molecular Biology 2021Quote: ... antibodies were detected using HRP-conjugated mouse secondary antibody (Promega). UAP56/DDX39B [1:2000] (custom generated51 ...
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bioRxiv - Cell Biology 2023Quote: Mouse livers were homogenized in Luciferase Culture Lysis Reagent (Promega). Lysates were clarified by centrifugation at 13,000 × g for 20 min at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... For Halo-tag labeling cells were allowed to re-attach for 1 hour under hypoxic conditions and then incubated with 0.5 uM of Janelia Fluor 549 (Promega) for 15 minutes ...
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bioRxiv - Microbiology 2021Quote: ... The supernatants of each well were collected then assessed using a Multitox-Fluor Multiplex Cytotoxicity Assay (Promega, Madison, WI), in accordance with the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... we normalized the luminescent signal to the fluorescent signal obtained with CellTiter-Fluor® Cell Viability assay (G6082, Promega). The signals were detected with Spark® multimode microplate (Tecan ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated for 20 min in 100 nM cell-permeable halotag conjugated to Janelia Fluor 549 dye (Promega) before washing twice with PBS ...
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bioRxiv - Microbiology 2023Quote: ... The RNA amount in the eluted fraction was quantified with Quanti Fluor RNA dye (E286A, Promega, Madison, WI, USA) by following the manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: ... HEK293 culture medium was replaced by imaging medium containing either Janelia Fluor 646 (JF646) ligand (10 nM; Promega, #GA1120) or OregonGreen ligand (50 nM ...
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bioRxiv - Cancer Biology 2021Quote: Each enhancer or promoter region was amplified from human genomic DNA and cloned into pGL4.10 [luc2] (Promega) containing a SNP (rs718960 ...
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bioRxiv - Neuroscience 2020Quote: ... and pCMVΔ8.9 plasmids were co-introduced into human embryonic kidney 293T cells using the FuGENE reagent (Promega). 48 h after transfection ...
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bioRxiv - Microbiology 2020Quote: ... albicans to damage human vascular endothelial cells was assessed by the CytoTox-96 assay (Promega, Madison, WI), which measures the release of lactate dehydrogenase (LDH ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were transfected with human RXFP1 pcDNA-Zeo-tetO and the GloSensor reporter plasmid using FuGENE (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... HEK 293T cells were co-transfected with human MOR and a luciferase-based cAMP biosensor (GloSensorTM, Promega). The next day ...
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bioRxiv - Genetics 2022Quote: We performed transient transfection on human pluripotent stem cells using FuGENE® HD Transfection Reagent (Promega, E2311). The cells were splitted and treated with Y-27632 for one before transfection.
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bioRxiv - Molecular Biology 2023Quote: ... 15 mg of His-Tev-HaloTag-(3C)-human γ-TuNA was coupled to Halo Magne beads (Promega). Xenopus laevis egg extract was prepared by standard methods28 ...
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bioRxiv - Genomics 2023Quote: ... Primer efficiency was calculated using a 10-fold serial dilution of Human Mixed Genomic DNA (Promega G3041). For primer sequences and calculated efficiencies ...
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bioRxiv - Cell Biology 2024Quote: All human cell lines were authenticated at each batch freezing by STR profiling (StemElite ID System, Promega). All cell lines were tested for mycoplasma at each batch freezing by PCR (32 ...
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bioRxiv - Cancer Biology 2020Quote: ... Relative cell number was determined by a fluorescence assay based on the measurement of a constitutive protease activity restricted to intact viable cells and that is independent of the cellular metabolic activity (CellTiter-Fluor, Promega). The fluorescence signal was kept within the linear range and was proportional to cell number ...
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bioRxiv - Genetics 2021Quote: ... cell viability followed by caspase 3/7 activity were measured using CellTiter-Fluor™ Cell Viability Assay kit (Promega, G6080) and Caspase-Glo® 3/7 Assay System (Promega ...