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Citations for Promega :
251 - 300 of 633 citations for Measles virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... we produced recombinant H3 mutant proteins from mRNAs using rabbit reticulocyte lysate (Promega L4600). After 3h of incubation at 4°C in interphase extracts followed by another 3h incubation with anti-HA beads ...
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bioRxiv - Microbiology 2019Quote: ... ATP was estimated from bacterial lysates using Bac Titer-GloTM Assay kit from Promega using manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Cell lysates (20μl) were mixed with 100 μl Renilla Luciferase Assay System Substrate (Promega). The luminescence level was measured and the relative light units (RLUs ...
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bioRxiv - Microbiology 2021Quote: ... 20 μL of the lysate and 10 μL of the luciferase substrate (Promega, E4550) were added into white 96-well plates for chemiluminescence detection using a Varioskan LUX Multimode Microplate Reader (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... 20 μL of the lysate and 10 μL of the luciferase substrate (Promega, E4550) were added into white 96-well plates for chemiluminescence detection using a Varioskan LUX Multimode Microplate Reader (ThermoFisher ...
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bioRxiv - Neuroscience 2021Quote: ... and 25 uL of lysate was mixed with ONE-Glo luciferase assay buffer (Promega) in opaque 96-well plates ...
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bioRxiv - Molecular Biology 2021Quote: In vitro translation reactions were performed using nuclease-treated rabbit reticulocyte lysate (RRL) (Promega) following the manufacturer’s protocol for non-radioactive luciferase reactions ...
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bioRxiv - Immunology 2021Quote: ... 20 μL lysate was added to 100 μL Luc substrate (Promega Luciferase Assay System). The amount of luciferase was quantified by luminescence (relative luminescence units (RLU)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lysates were obtained using lysis buffer provided in Dual Luciferase Assay Kit (Promega). Firefly and Renilla luciferase readings were recorded 48 hours posttransfection using Luminometer (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: Fifty μl protein expression reaction using TNT SP6 Coupled Reticulocyte Lysate System (Promega, L4600) containing 1000 ng pIX-Halo-bZIP plasmids was incubated for 3 h at 30°C ...
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bioRxiv - Cell Biology 2023Quote: ... using the rabbit reticulocytes lysates Nuclease-treated kit and the transcendTM tRNA from Promega, as per the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were examined for luciferase activity using a commercial kit assay (Promega Cat.# E2810). Lysates (20 µL ...
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bioRxiv - Microbiology 2023Quote: ... and 50 µl cleared lysate was mixed with 50 µl Bright Glo reagent (Promega) for determination of viral RNA synthesis/protein expression or 50 µl CellTiter-Glo reagent (Promega ...
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bioRxiv - Microbiology 2024Quote: ... Luminescence in supernatants and cell lysates was determined using the Nano-Glo Substrate (Promega) and measured in a GloMax Explorer (Promega ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 μl of cell lysate was added to 20 μl of Luciferase Substrate (Promega), rotationally mixed for 15 minutes at RT and luminescence quantified on a FLUOstar Omega plate reader (BMG Labtech) ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were analyzed by LC-MS or with the NAD/NADH-Glo kit (Promega). B ...
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bioRxiv - Plant Biology 2020Quote: ... corniculatus plants was synthesized from 3 μg of total RNA using a Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) (Promega, WI, USA) and 100 pmol of random hexamers (Pharmacia Biotech) ...
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bioRxiv - Molecular Biology 2021Quote: cDNA synthesis for gene expression analyses was performed using 500 ng RNA using Moloney murine leukemia virus (M-MLV) reverse transcriptase and random hexamer oligonucleotide (both from Promega, Southampton, UK) using 500 ng RNA in a 25 µl reaction ...
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bioRxiv - Biochemistry 2020Quote: ... Moloney murine leukemia virus reverse transcriptase (MMLV-RT) enzyme and recombinant RNasin® ribonuclease inhibitor were purchased from Promega (Madison, WI, USA). Evagreen 2X qPCR MasterMix was purchased from Applied Biological Materials Inc ...
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bioRxiv - Cell Biology 2021Quote: ... and 1-3μl aliquots were taken for reverse transcription (RT) using the Moloney murine leukemia virus RT RNase H (−) (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... The resulting materials were used as template to generate cDNA using 100 U Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI), 0.45 μM N6 random hexamer primers (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: DNA was extracted from this concentrated virus sample using a modified protocol with the Wizard Genomic DNA Purification Kit A1120 (Promega, Madison, WI). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... virus replication was quantified by measuring Renilla luciferase activity (relative light units [RLU]) using Bright-Glo™ Luciferase Assay System (Promega, USA) 48 hours post-reinfection ...
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bioRxiv - Microbiology 2019Quote: ... Virus infectivity was quantified by measuring Renilla luciferase activity (relative light units [RLU]) using Bright-Glo™ Luciferase Assay System (Promega, USA) on the Tecan microplate reader (Tecan Infinite® 200 Pro ...
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bioRxiv - Cell Biology 2020Quote: ... Total 500 ng RNA was used to synthesize cDNA by using Moloney-murine leukemia virus (M-MLV) reverse transcriptase (Promega, Madison, WI). Diluted cDNA was used in each quantitative reverse transcription-PCR (qRT-PCR) ...
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bioRxiv - Plant Biology 2022Quote: ... cDNAs were synthesized from 1.5 μg of total RNA with oligo(dT) primers using Moloney Murine Leukemia Virus Reverse Transcriptase MMLV-RT according to the manufacturer’s instructions (Promega, Madison, WI, USA). RT-qPCR was carried out in a Chromo4 system (Bio-Rad ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... The plates were incubated at 37°C under 5% CO2 for 144 hour and virus cytopathic effect was measured using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, Cat. # G9241) following the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... the amount of input virus was normalized to the HiBiT value measured by Nano Glo HiBiT lytic detection system (Promega, Cat# N3040)] ...
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bioRxiv - Microbiology 2023Quote: ... Samples where a 1 log decrease in RLU compared to the no-serum virus-only control was observed were then selected for cytotoxicity assay using Cell Titre Glo kit (Promega, Southampton, UK) and light microscopy to verify viability of cells prior to undertaking PVNA (data not shown) ...
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bioRxiv - Microbiology 2024Quote: ... followed by reverse transcription to complementary DNA (cDNA) using 30 enzyme units(U) of the Moloney Murine Leukemia Virus reverse transcriptase (MMLV-RT) (Promega, WI, USA). The extension products were then analysed using the ABI3730xl DNA Analyzer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Luciferase activity of cell lysate was measured using the Nano-Glo Luciferase Assay System (Promega) and GloMax Discover System ...
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bioRxiv - Synthetic Biology 2021Quote: ... The luciferase activity in the cell lysates was measured using a GloMax Navigator system (Promega). All luciferase assays were conducted in LumiNunc 96-well white plates (Thermo Scientific ...
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bioRxiv - Microbiology 2019Quote: Each lysate was incubated with an equal volume of firefly luciferase reagent (Promega, Cat. #E1500) for 1 min in a 96-well white-walled plate (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... A master mix of 3.5uL lysate with 0.5uL 100uM complete amino acid mix (Promega L4461) and 1uL 10x translation buffer (20mM Hepes pH 8 ...
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bioRxiv - Immunology 2021Quote: ... Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with the Glomax Navigator (Promega) ...
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bioRxiv - Plant Biology 2020Quote: ... constructs were translated in vitro using a TNT (TM) Coupled Rabbit Reticulocyte Lysate kit (Promega). His-mPlsp1ΔTMD ...
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bioRxiv - Immunology 2021Quote: ... Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with the Glomax Navigator (Promega) ...
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bioRxiv - Immunology 2021Quote: ... NanoLuc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega). Relative luminescence units (RLUs ...
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bioRxiv - Cancer Biology 2021Quote: ... luciferase activity in total cell lysates was measuring using Dual-Luciferase Reporter Assay Kit (Promega) and normalized for transfection efficiency via dividing by the Renilla luciferase activity ...
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bioRxiv - Cell Biology 2020Quote: ... β-gal expression was simultaneously determined from the lysates according to the manufacturer’s protocol (Promega, E2000). Differences in transfection efficiency were accounted for by normalizing Luc expression to β-Gal expression (Luc/β-Gal) ...
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bioRxiv - Immunology 2022Quote: ... Luciferase signal was determined from cell lysates by adding the Bright-Glo Luciferase buffer (Promega) and subsequent analysis using a Glomax plate reader ...
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bioRxiv - Molecular Biology 2022Quote: ... Whole cell lysates were prepared and luciferase activity was measured using a kit from Promega.
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bioRxiv - Immunology 2022Quote: ... Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with the Glomax Navigator (Promega) ...
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bioRxiv - Immunology 2022Quote: ... Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with the Glomax Navigator (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... cells were lysed with NanoGlo substrate and lysates were quantified in a GloMax Explorer (Promega) according to manufacturer’s instructions for all competitive inhibition assays.
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bioRxiv - Genetics 2022Quote: ... Cell lysate was used in dual luciferase assays using the Dual-Glo luciferase system (Promega), measured on the GloMax Discover plate reader (Promega) ...
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bioRxiv - Immunology 2021Quote: ... NanoLuc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega). Specifically ...
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bioRxiv - Immunology 2020Quote: ... Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with a GloMax Natigator Microplate Luminometer (Promega) ...
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bioRxiv - Cell Biology 2019Quote: ... luciferase activity was measured in cell lysates using the Dual-Luciferase reporter assay system (Promega) normalized to Renilla luciferase activity.
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bioRxiv - Microbiology 2020Quote: ... NanoLuc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega). Specifically ...