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Citations for Promega :
51 - 100 of 633 citations for Measles virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... and then used for reverse transcription with avian myeloblastosis virus (AMV) reverse transcriptase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The virus-induced CPE was assessed using the Viral ToxGlo™ assay (Promega #G8943) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) system for RT-PCR (Promega) was used for cDNA synthesis using 500 ng extracted RNA from all samples ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized with Moloney murine leukemia virus (MMLV) reverse transcriptase (Promega, Madison, WI). qRT-PCR was performed with the BioRad CFX96 Real-Time PCR System or Applied Biosystems 7000 ...
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bioRxiv - Molecular Biology 2024Quote: ... An equal amount of extension mix consisting of avian myeloblastosis virus reverse transcriptase (Promega), 5x AMV buffer and 40 μM dNTPs was added ...
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bioRxiv - Systems Biology 2019Quote: ... To reduce the viscosity of the lysate and remove contaminating DNA the lysate was incubated with RQ1 DNase I (10U/ml Promega, M6101) for 30 minutes on ice ...
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bioRxiv - Biochemistry 2020Quote: ... Complementary DNA was reverse-transcribed using Moloney murine leukemia virus reverse transcriptase (Promega, Wisconsin, USA). Relative expression of genes of interest were quantified by real-time PCR using TaqMan™ gene expression assays (Mm02619580_g1 ACTB ...
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bioRxiv - Plant Biology 2021Quote: ... and 2μg RNA were transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (Promega). A 20-fold dilution of the cDNA reaction was used for PCR amplification with the ABsolute QPCR SYBR Green Capillary Mix (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and cDNA was produced with Moloney murine leukemia virus reverse transcriptase (M-MLV RT) (Promega) and random hexamers ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... RNA was then reverse transcribed using Moloney-Murine Leukaemia Virus (M-MLV) reverse transcriptase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and DNase-I digested before murine leukemia virus (MLV) reverse transcription using random primers (Promega). qPCR was performed using the TaqMan technology (Life Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: The clarified lysate was incubated with HaloLink Resin (Promega) for 15min at 22°C with rotation ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates were harvested in Passive Lysis Buffer (Promega) and analyzed with the Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates were harvested in Reporter Lysis Buffer (Promega) and analyzed with the Luciferase Assay System (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... Nuclease-treated rabbit reticulocyte lysate translation reactions (Promega L4960) were set up in a 384-well plate (Thermo Scientific 164610 ...
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bioRxiv - Biochemistry 2021Quote: ... were performed in nuclease-treated rabbit reticulocyte lysate (Promega) as previously described (Zong et al. ...
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bioRxiv - Cell Biology 2020Quote: ... NDUF13 precursors were synthesized using rabbit reticulocyte lysate (Promega) in the presence of [35S] methionine ...
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bioRxiv - Molecular Biology 2022Quote: 1 ml nuclease-untreated Rabbit Reticulocyte Lysate (Promega L4151) was supplemented with 25 µM haemin ...
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bioRxiv - Biochemistry 2021Quote: ... 17.5 µL of nuclease-treated rabbit reticulocyte lysate (Promega), 5 U RNase inhibitor (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... Nuclease-treated rabbit reticulocyte lysate was from Promega (L4960), [35S] methionine (EasyTag EXPRESS 35S Protein Labelling Mix ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were incubated with Stop & Glo substrates (Promega) and luciferase activity was measured in a Synergy Neo2 reader (Biotek).
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bioRxiv - Microbiology 2020Quote: ... the TnT® Coupled Reticulocyte Lysate system (Promega; #L4610) was utilised as per the manufacturers’ instructions using 200ng of plasmid DNA ...
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bioRxiv - Immunology 2021Quote: ... All transcripts were translated in rabbit reticulocyte lysate (Promega) in the presence of L-35S-methionine (Perkin Elmer) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... each sample was added to Rabbit Reticulocyte Lysate (Promega) and incubated at 37°C for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 47.5 µL of nuclease-treated rabbit-reticulocyte lysate (Promega), and one of four concentrations of HR1 peptide (0 ...
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bioRxiv - Microbiology 2022Quote: ... were performed in nuclease-treated rabbit reticulocyte lysate (Promega) as described53,54 ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were prepared using Passive lysis buffer (Promega). Supernatants were collected and measured for luciferase activities using the Dual luciferase reporter assay system (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... transcripts were incubated with rabbit reticulocyte lysates (L4960; Promega) for 30 min at 37°C.
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bioRxiv - Biochemistry 2024Quote: ... Normalized cell lysate was first mixed with vivazine (Promega) to reach 1% vol/vol as recommended by Promega ...
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bioRxiv - Immunology 2022Quote: Viral RNA was extracted from virus-infected culture medium by using Maxwell RSC Instrument (Promega, AS4500). cDNA preparation and amplification were done in accordance with protocols published by the ARTIC network (https://artic.network/ncov-2019 ...
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bioRxiv - Molecular Biology 2021Quote: ... Renilla luciferase is driven by a constitutively active simian virus 40 (SV40) promoter (pRL-SV40; Promega), which served as a control for cell viability and transfection efficiency ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Virus infectivity was assayed by measuring chemiluminescent firefly luciferase activity using the commercial kit from Promega.
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bioRxiv - Microbiology 2021Quote: All virus stocks were produced by plasmid transfection of HEK 293T cells with Fugene 6 (Promega). Supernatants were harvested at 48h and 72h ...
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bioRxiv - Cell Biology 2021Quote: ... as much liquid was removed as possible and HaloTEV (Tobacco Etch Virus) protease (300 units, Promega) was added to liberate protein ...
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bioRxiv - Cell Biology 2022Quote: The SARS-CoV-2 spike pseudotyped virus activity was determined by bright-glo luciferase assay (Promega). The plate reader detected the luminescence two days post virus infection or without virus infection ...
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bioRxiv - Microbiology 2020Quote: The total RNA was reverse transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (Promega). Real-time PCR was performed using the POWER SYBR green PCR master mix (Applied Biosystems ...
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bioRxiv - Biochemistry 2021Quote: ... Reverse transcription was performed with 1 unit of Avian Myoblastosis Virus (AMV) reverse transcriptase (Promega®) at 42°C for 2 min ...
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bioRxiv - Immunology 2019Quote: ... Reverse transcription (RT) was carried out using 2.5 U avian myeloblastosis virus (AMV) reverse transcriptase (Promega) and a 32P-labelled primer in the presence of 4 mM MgCl2 and 0.5 mM dNTPs ...
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bioRxiv - Microbiology 2020Quote: ... Cells transduced with luciferase expressing virus were assessed using Promega Steady-Glo system (Promega Madison, WI)
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was then performed with 50 U of Avian Myeloblastosis Virus reverse transcriptase (Promega Corporation) for 20 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: Reverse transcription was performed using the Moloney Murine Leukemia Virus Reverse Transcriptase (Promega, Madison, WI, USA), 1000 ng of total RNA and random hexamers ...
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bioRxiv - Immunology 2023Quote: ... Pseudotyped virus were prepared by transfection of HEK293T cells using the Fugene HD transfection system (Promega), as previously described(42) ...
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bioRxiv - Molecular Biology 2021Quote: 500 µl rabbit reticulocyte lysate obtained from Promega (L4151, untreated) was supplemented with 1 µl 12.5 mM Hemin (Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... using the TnT Quick-Coupled reticulocyte lysate system (Promega #L1170) and 35S-Methionine (Perkin Elmer #NEG709A001MC) ...
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bioRxiv - Cell Biology 2019Quote: ... and lysates were analyzed using a GloMax® Luminometer (Promega). Transfection and expression efficiencies were normalized to the activity of β-galactosidase activities.
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Selective inhibition reveals the regulatory function of DYRK2 in protein synthesis and calcium entrybioRxiv - Biochemistry 2021Quote: ... The cell lysates were firstly digested with trypsin (Promega, USA) by the in-solution digestion method22 ...
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bioRxiv - Microbiology 2021Quote: ... the protein lysates were digested with trypsin (Promega, Madison, WI) at 37°C overnight and the digested samples were desalted with 1cc C18 Sep-Pak solid phase extraction cartridges (Milford ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were prepared in 1 × Reporter lysis buffer (Promega, E2510), and Luciferase assays were performed according to the manufacturer’s protocol (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... In vitro translation was performed in rabbit reticulocyte lysate (Promega) supplemented with 1 mCi/ml [35S]Met/Cys ...
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bioRxiv - Microbiology 2021Quote: Translation was performed in Flexi® Rabbit Reticulocyte Lysate (Promega) supplemented with amino acid mixture minus methionine and leucine ...