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Citations for Promega :
501 - 550 of 633 citations for Measles virus lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The identity of each cell line was confirmed by DNA fingerprinting via short tandem repeats at the time of mRNA and total protein lysate preparation using the PowerPlex 1.2 kit (Promega).
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bioRxiv - Cell Biology 2020Quote: ... 20 µL lysates were loaded into a 96 well plate and luminescence following administration of 50 µL Luciferase Assay Reagent II (Promega) and 50 µL Stop & Glo Reagent (Promega ...
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bioRxiv - Immunology 2021Quote: Cells were washed twice in D-PBS (Wisent) and lysates were collected using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... After 24 h luciferase activity was determined from at least four independent experiments with 20 μl of cleared lysate in an LB 9501 luminometer (Berthold) by using the luciferase assay system from Promega.
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bioRxiv - Microbiology 2020Quote: ... NPs and Su9-DHFR were in vitro translated using rabbit reticulocyte lysate with either the TnT SP6 quick couple Transcription/Translation System (Promega) for cDNA sequences flanking the SP6 promoter ...
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bioRxiv - Molecular Biology 2021Quote: ... and luciferase activity in 10 μl aliquots of the cell lysates was measured using the Dual Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocols ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Reporter gene expression was assessed in 20 µl of cell lysates using the Dual Luciferase Assay and a GloMax luminometer (Promega). To control for transfection efficiency ...
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bioRxiv - Neuroscience 2020Quote: In vitro translation rates represent the increase in luminescence as a function of time using a standard rabbit reticulocyte lysate system (Promega) with luciferase mRNA (Promega) ...
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bioRxiv - Molecular Biology 2020Quote: ... ATFS-11-100(R/R)::GFP and Su91-69::GFP) were synthesized in reticulocyte lysate in the presence of [35S]methionine (T7 Quick for PCR DNA, Promega). Import into isolated mitochondria was performed in import buffer (3 % (w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μL of lysate was diluted with 39 μL PBS before being mixed with 40 μL Dual-Glo FLuc substrate (Promega). After 10 min ...
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bioRxiv - Microbiology 2020Quote: ... cell viability was assessed by measruring ATP levels in cells lysates with a commercially available kit (CellTiter-Glo®, Promega).
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bioRxiv - Microbiology 2020Quote: ... Transduction rates were assessed by measuring luciferase activity in cell lysates at 48 hours post transduction with a commercially available kit (Promega). Values for untreated controls were set to 100% infection.
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bioRxiv - Microbiology 2020Quote: ... Infection rates were assessed by measuring luciferase activity in cell lysates at 16 h post transduction with a commercially available kit (Promega). Values for untreated controls were set to 100% infection.
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bioRxiv - Genomics 2019Quote: ... Firefly luciferase and Renilla luciferase activities were measured in 10μl of HEK293T cell lysate using the Dual-Luciferase Reporter assay system (Promega, E1910) in a Veritas™ Microplate Luminometer ...
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bioRxiv - Microbiology 2019Quote: In vitro translation reactions were performed using coupled bacteriophage T7 RNA polymerase transcription-rabbit reticulocyte lysate translation reactions as per the manufacturer’s instructions (Promega TNT). Samples were radiolabelled with 35S-methionine (Perkin Elmer ...
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bioRxiv - Biochemistry 2020Quote: All basic transcription-translation reactions for PrP constructs were performed using a Rabbit Reticulocyte Lysate (RRL) derived SP6 coupled system (Promega) for 30 min at 30 °C in the presence of 2.5 μCi [35S]-methionine in a total volume of 10 μl ...
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bioRxiv - Cell Biology 2020Quote: ... 10 g of the muscle lysates were used for determining proteasome-associated chymotrypsin-like activity with the Proteasome-GloTM Assay Systems (#G8531, Promega). Luminescence signals were detected with the SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices ...
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bioRxiv - Cancer Biology 2020Quote: ... 20 μL of cell lysate was transferred to 96-well plates and placed in a 96-well microplate luminometer (GloMax-Multi, Promega). 100 μL Luciferase Assay Reagent II and 100 μL Stop & Glo Reagent (both provided in the Dual-Luciferase Reporter kit ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell lysates were transferred to a 96-well black plate and then an equal volume of Luciferase assay reagent (Promega) was added ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 10 μL of lysate was diluted with 30 μL PBS before being mixed with 40 μL Dual-Glo FLuc substrate (Promega). After 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... the luciferase activities were measured in 20 μl of cell-lysate using the dual-luciferase assay system (Promega, Madison, WI) with a luminometer (LumiCount ...
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bioRxiv - Synthetic Biology 2021Quote: All NanoLuc luciferase assays to quantify gene expression were performed with cell lysate according to the procedure described in the Nano-Glo Luciferase Assay System kit (Promega). For these experiments ...
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bioRxiv - Systems Biology 2021Quote: ... Collisional energy was ramped stepwise as a function of ion mobility.24 Peptide loading was normalized to the total ion chromatograms from 200 ng injections of K562 whole cell lysate standard (Promega).
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bioRxiv - Plant Biology 2021Quote: [35S]Met-labeled preproteins were in vitro-transcribed/translated using the TNT-coupled wheat germ or reticulocyte lysate system and SP6 or T7 RNA polymerase (Promega). Growth of Arabidopsis seedlings (for 14 days on MS agar media with 2% sucrose) ...
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bioRxiv - Microbiology 2020Quote: ... the transduction efficiency was quantified by measuring the firefly luciferase activity in cell lysates using the firefly luciferase substrate of the dual-luciferase reporter assay system (Promega) and a Glowmax plate luminometer (Promega).
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bioRxiv - Molecular Biology 2021Quote: ... Luciferase assays were performed on 20 μl of lysate or 1x Cell Culture Lysis Reagent in 96 well plates on the GloMax-Multi Detection System (TM297, Promega). 100 μl Luciferase Assay Reagent was added to wells ...
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bioRxiv - Microbiology 2020Quote: ... and luciferase activity was measured in 3 µl aliquots of the cell lysates using a Dual-Luciferase Reporter Assay System (Promega). Firefly luciferase activity was normalized with that of Renilla luciferase.
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bioRxiv - Microbiology 2020Quote: ... at 9 h post-electroporation and luciferase activity was measured in 10 µl aliquots of the cell lysates using a Luciferase Reporter Assay System (Promega).
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bioRxiv - Cancer Biology 2021Quote: ... luciferase assay reagent was mixed in a 1:1 ratio with cell lysate (Luciferase Assay System kit, Promega, Madison, WI). Luciferase activity was measured with Synergy H4 Hybrid Reader (BioTek ...
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bioRxiv - Cell Biology 2021Quote: ... 140 mM NaCl and 1% Triton X-100) and lysates used to assay luciferase activity in 96-well plates using the Dual-Luciferase Reporter Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... levels of Firefly and Renilla luminescence in cell lysates were measured by Synergy H1 microplate reader according to Promega’s Dual-Glo kit protocol (Promega #E2920). Fold changes of AP-1 activity were presented by the ratio of normalized Firefly/Renilla to control wells.
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bioRxiv - Biochemistry 2022Quote: Radiolabeled proteins were synthesized in the presence of 35S-methionine and 35S-cysteine using either coupled reticulocyte lysate system (TNT, Promega) or uncoupled reticulocyte lysate system (Promega) ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysate was washed in Nuclei wash/resuspension buffer (1xPBS completed with 1% BSA and 0.2U/ul RNAsin Plus (Promega, #N2611) and passed through a 40 μm cell strainer ...
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bioRxiv - Cell Biology 2022Quote: The T7-AKAP12 vector or its mutants were in vitro translated using the non-radioactive TNT® Coupled Transcription/Translation system containing rabbit reticulocyte lysate (RRL) and a biotin-lysyl tRNA according to the manufacturer’s instructions (Promega, WI) to incorporate biotin label into the translated AKAP12 protein ...
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bioRxiv - Bioengineering 2020Quote: ... Cas13a mRNA was translated in vitro using the Rabbit reticulocyte lysate system according to the manufacturer’s instructions (Promega, WI, USA). RNaseAlert®-1 Substrate was mixed with crRNA or NTCR (500 nM ...
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bioRxiv - Microbiology 2022Quote: ... then cells were harvested and cell lysates were used to determine luciferase using a Dual-Luciferase Reporter Assay System (Promega). The firefly luciferase activities were normalized to Renilla luciferase activities.
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bioRxiv - Immunology 2022Quote: ... followed by a PBS wash and lysis buffer to measure the luciferase activity in cell lysates using the Nano-Glo Luciferase Assay System (Promega) and GloMax system (Turner BioSystems) ...
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bioRxiv - Immunology 2019Quote: 8 nM cap0 or 20 ng/μL VPg-linked RNA was translated using the Flexi Rabbit Reticulocyte Lysate system (Promega) in the presence or absence of 1.5 μM Ifit proteins ...
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bioRxiv - Biochemistry 2019Quote: ... in vitro translated hnRNP M protein fragments (5 μl) produced by rabbit reticulocyte lysate using TNT Quick Coupled Transcription/Translation Systems (Promega). Proteins were incubated at 4°C for 2 hours in the binding buffer above ...
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bioRxiv - Microbiology 2019Quote: ... and both firefly and Renilla luciferase activities in the lysate were determined using the Bright-Glo and Renilla-Glo Luciferase Assay System (Promega), respectively ...
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bioRxiv - Microbiology 2020Quote: ... The former cell lysates were subjected to a luciferase assay to normalize the transfection efficiency using a firefly Luciferase Assay System (Promega) with a Centro LB960 luminometer (Berthold) ...
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bioRxiv - Immunology 2020Quote: ... The activity of Renilla luciferase in total cell lysates was measured with the use of a Dual-Luciferase Reporter Assay System (Promega) and was normalized by that of firefly luciferase derived from 80 ng of a control plasmid.
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bioRxiv - Immunology 2020Quote: ... the cells were lysed and luciferase activity was measured in the lysates using the Nano-Glo Luciferase Assay System (Promega). Relative luminescence units (RLU ...
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bioRxiv - Molecular Biology 2020Quote: ... Western blots to assess the abundance of the proteins were performed on the same volumes of lysates obtained from the luciferase measurements using the passive lysis buffer supplied with the Dual-Luciferase Reporter Assay System (Promega). Antibodies used for western blots were previously described (Grozdanov et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Firefly (Fluc)- and Renilla (Rluc)-luciferase activity in each cell lysate was determined using the Dual Luciferase Assay Kit (Promega) and a Berthold tube luminometer ...
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bioRxiv - Biochemistry 2020Quote: ... the culture medium was aspirated and 20 μL of cell lysate was added from the Luciferase Assay System (Promega, E1500) to each well ...
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bioRxiv - Immunology 2020Quote: ... the cells were washed with PBS and then lysates were prepared by scraping the cells from plates in the presence of 1× Passive lysis buffer (Promega). Luciferase assays were performed according to Dual-Luciferase Assay System (Promega ...
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bioRxiv - Developmental Biology 2020Quote: ... lysed in 100µl of passive lysis buffer and standard dual luciferase assays were performed on the cell lysates by using Dual-Luciferase Reporter Assay reagents (Promega).
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were prepared to consecutively measure the firefly and Renilla luciferase activities using Dual-luciferase reporter assay system (Promega) on INFINITE 200 Pro multimode reader (TECAN) ...
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bioRxiv - Cell Biology 2022Quote: ... Tg-NLuc abundance in the lysate and media were measured using the nano-glo luciferase assay system according to the manufactures protocol (Promega). Four controls were included for the experiments including a non-targeting siRNA control ...