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Citations for Promega :
351 - 400 of 1480 citations for Human PLA2G4F shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and CAR plasmids were treated with FuGENE (Promega, E2311) transfection reagent and then lentivirus production was performed using host cells HEK293T [10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Bioengineering 2022Quote: ... and pGL4.51 [luc2/CMV/Neo] plasmid vector (Promega, E1320) as per the manufacturer’s specifications and incubated with K7M2 cells for 24 hours ...
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bioRxiv - Synthetic Biology 2022Quote: ... or the PureYield™ Plasmid Maxiprep System (Promega, #A2393) for transfection and in vitro transcription applications ...
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bioRxiv - Neuroscience 2023Quote: ... The Atp6v1h promoters were cloned into pGL3 plasmid (Promega). The CLEAR mutant promoters were generated using site-directed mutagenesis (QuikChange II XL Site-Directed Mutagenesis Kit ...
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bioRxiv - Microbiology 2023Quote: ... and cloned into the pCI plasmid (Promega, Madison, WI). Large-scale DNA plasmid was amplified in Escherichia coli DH5α and purified by using a plasmid giga prep kit (Zymo Research ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... on linearized plasmids encoding codon-optimized NanoLuc Luciferase (Promega), Cre recombinase ...
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bioRxiv - Molecular Biology 2023Quote: ... and ligated into the pSiCheck2 plasmid (Promega Cat #C8021) where renilla luciferase is upstream of the 3’UTR and firefly luciferase is driven by a separate promoter as a reference control ...
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bioRxiv - Biophysics 2023Quote: ... The pCMV-LgBiT expression plasmid was purchased from Promega. pcDNA3.1-hACE2-C9 was obtained from Dr ...
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bioRxiv - Cell Biology 2023Quote: ... The pSV40-Renilla plasmid was purchased from Promega (E2231). To assess the activity of NF-κB ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli by miniprep (PureYield™ Plasmid Miniprep System, Promega), ethanol precipitated ...
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bioRxiv - Immunology 2023Quote: ... and REN-Luc/pRL-TK plasmid (20 ng; Promega) expressing Renilla luciferase used as an internal control to normalize transfection efficiency ...
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bioRxiv - Cell Biology 2023Quote: ... pRL-TK and pGL4-54 plasmids were from Promega and inserts synthesised by Eurofins ...
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bioRxiv - Developmental Biology 2024Quote: ... performed according to the PureYield Plasmid Miniprep System (Promega) instructions ...
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bioRxiv - Molecular Biology 2023Quote: Plasmid DNA transfections were performed using FuGENE 6 (Promega), Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Biophysics 2024Quote: ... The plasmid was transformed into Rosetta (DE3) cells (Promega). Protein expression was induced by the addition of 0.25 mM isopropyl ®-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lines were transfected with plasmids using Viafect (Promega) in 6 well plates according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: The CD19 minigene was amplified from human genomic DNA (Promega) with the primers 5’-catAAGCTTgaccaccgccttcctctctg-3’ and 5’-catGAATTCNNNNNNNNNNNNNNNGGATCCttcccggcatctccccagtc-3’ ...
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bioRxiv - Neuroscience 2020Quote: ... the human CB1R was cloned into the pCI vector (Promega), and eCB2.0 and eCBmut were cloned into the peGFP-C1 vector (Takara) ...
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bioRxiv - Immunology 2022Quote: ... and a β-globin calibration curve (human genomic DNA [Promega]) was concurrently evaluated on each plate ...
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bioRxiv - Microbiology 2020Quote: ... The human codon-optimized NanoLuc Luciferase reporter gene (Nluc, Promega) was inserted in place of nucleotides 1-100 of the nef-gene ...
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bioRxiv - Genomics 2022Quote: ... 10 ng/mL recombinant human basic fibroblast growth factor (Promega), and 1 μM dexamethasone (Sigma-Aldrich) ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... donovani BPK282/0 cl4 promastigote DNA with human DNA (Promega) from 1/15 to 1/150000 (Leishmania:human ...
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bioRxiv - Biochemistry 2020Quote: hnRNP L and SETD2 human ORF were procured from Promega. Deletion mutants of hnRNP L and SETD2 were constructed by PCR (Phusion polymerase ...
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bioRxiv - Immunology 2020Quote: ... The human codon-optimized nanoluc Luciferase reporter gene (Nluc, Promega) was inserted in place of nucleotides 1-100 of the nef-gene ...
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bioRxiv - Molecular Biology 2022Quote: ... Serial dilutions of genomic DNA from human placenta (G1471, Promega) were used as a standard for quantification and their concentration.
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bioRxiv - Microbiology 2022Quote: ... falciparum 3D7 DNA with commercially available human DNA (Promega, G304A) to a total amount of 400ng DNA ...
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bioRxiv - Neuroscience 2024Quote: Human male genomic DNA obtained from Promega (Ref:G1471; Madison, WI) was used to generate the input library ...
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bioRxiv - Biochemistry 2022Quote: ... transfer plasmid was co-transfected with GAG/POL and VSVG plasmids into HEK293T or 293FT cells using FuGENE HD (Promega, 3.33:1 FuGENE:DNA) and the medium was replaced 6–8 h after transfection ...
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bioRxiv - Immunology 2022Quote: The whole-genome sgRNA library plasmid (GeCKOv2, a gift from David Huang, University of Melbourne) plus packaging plasmids using FuGENE6 (Promega, cat. no. E2691) were transfected into low-passage HEK293T cells at 80% confluency in 15 cm tissue culture dishes ...
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bioRxiv - Molecular Biology 2023Quote: ... containing 300 ng of the recombinant T/F LTR-pGL3 plasmid and 100 ng of recombinant control insert DNA-pTarget plasmid (Promega Corporation, Madison, WI) for the Tat minus transfection or 100 ng of recombinant HIV-1C consensus or autologous Tat-pTarget plasmid (obtained from the same sample from which the LTR was derived ...
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bioRxiv - Molecular Biology 2022Quote: Transient transfection of plasmids was performed with Fugene HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and the expression plasmid of interest using Fugene HD (Promega). FLuc and RLuc activity were quantified using the Dual Luciferase Assay Kit (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... reporter plasmids (500 ng/ml) were transfected using FugeneHD (Promega). 24h after transfection luciferase levels determined using Promega dual luciferase assay and GloMax plate reader (Promega).
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bioRxiv - Cancer Biology 2019Quote: ... and pRL-CMV (a Renilla luciferase plasmid, Promega, Madison, WI) plasmids were transfected into HEK293T cells (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... and colonies were selected for plasmid purification (PureYield protocol, Promega). PCR products were then sequenced (Eurofins Genomics ...
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bioRxiv - Cell Biology 2020Quote: ... The pRLCMV reporter plasmid was obtained from (Promega, Madison, USA). The pmRFP-C1 and pEGFP-Rab7 plasmid were obtained from Dr Andrew Gilmore (Faculty of Life Sciences ...
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bioRxiv - Molecular Biology 2019Quote: ... the Renilla and Firefly luciferase genes in psiCheck2 plasmid (Promega) were first replaced by constructs with synonymous mutations in putative m6A sites (obtained as IDT gBlocks) ...
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bioRxiv - Cancer Biology 2019Quote: ... Plasmids were forward transfected into cells using FuGENE 6 (Promega). BEZ-235 ...
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bioRxiv - Cell Biology 2019Quote: ... coli and purified with the PureYield Plasmid Miniprep System (Promega). The plasmids were then transfected into HEK293T cells using jetPRIME transfection reagent (Polyplus Transfection ...
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bioRxiv - Cell Biology 2019Quote: ... Where indicated cells were transfected with plasmids using FugeneHD (Promega). C10 (del ΔH ...
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bioRxiv - Biophysics 2019Quote: ... expression plasmids were supplemented with empty vector DNA (pSI; Promega) to maintain the same ZP subunit/total DNA ratio.
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bioRxiv - Cell Biology 2019Quote: ... Plasmid transfections were performed with FuGENE 6 (Promega, cat#E2691) according to manufacturer instructions ...
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bioRxiv - Genomics 2019Quote: ... splice junction amplicons were cloned in pGEM-T plasmid (Promega) and subjected to Sanger sequencing ...
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bioRxiv - Neuroscience 2020Quote: ... IgSF8-EGFP and Tenascin-R-EGFP plasmids using Fugene6 (Promega). Twenty-four hours after transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Large scale plasmid generation was performed using Maxiprep columns (Promega), with purified plasmid used as input for lentiviral packaging with second generation packaging plasmids psPAX2 and pMD2.G (Cellecta) ...
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bioRxiv - Genomics 2021Quote: ... Transfection of plasmid (1μg) was done with FuGene HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Plasmid and siRNA transfections were done with Fugene 6 (Promega) and Lipofectamine RNAiMAX (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: The pGL3 reporter plasmid containing the SV40 early promoter (Promega) was used for all luciferase reporter assays along with an internal control Renilla plasmid (Promega) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The plasmid for luciferase (pBEST-Luc) was obtained from Promega. DNA for cell-free reactions was prepared from overnight bacterial cultures using Maxiprep kits (Macherey-Nagel).
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bioRxiv - Cell Biology 2022Quote: ... Plasmid transfection into RPE1 cells was performed using ViaFect (Promega) according to the manufacturer’s instruction.