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Citations for Promega :
301 - 350 of 1480 citations for Human PLA2G4F shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... HEK293 cells were transfected with the ORF8 plasmid together with the transposase coding plasmid (1/10) with Fugene HD (Promega, Germany) and after one day selected with 3 µg/ml puromycin for three days ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... High quality plasmid preparations for expression of ACE2-Fc variants were prepared using the PureYield Plasmid Midiprep System (Promega, United States). Transient transfection of the cells was performed at a cell density of approximately 1.7×106 cells mL-1 culture volume using a total of 1 μg of plasmid DNA and 2 μg of linear 40-kDa polyethylenimine (Polysciences Inc. ...
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bioRxiv - Immunology 2021Quote: Pseudoviruses expressing complete SARS-CoV2 spike genes were prepared by transient transfection of HEK293T cells with three plasmids: SARS-CoV2 MLV-gag/pol and MLV-CMV-luciferase plasmids using Fugene 6 (Promega Inc.) as described earlier (Rogers et al. ...
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bioRxiv - Immunology 2023Quote: ... a total of 50.000 chicken DF-1 cells were seeded in 24 well plates and were co-transfected 24h later with a vector plasmid containing the deletion mutant and a second plasmid for the expression of Firefly under the PGK promoter (Promega, USA). 24h after transfection ...
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bioRxiv - Developmental Biology 2023Quote: ... Pax2 packaging plasmid (7ug) and VSVG envelop plasmid (1ug) were mixed with 45ul of Fugene HD transfection reagent (Promega, cat#E5911) in 1.5ml of Opti-MEM (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected the following day using 0.6–1 µg of plasmid and Viafect reagent (Promega E4981, 6 µl/µg plasmid). The medium was exchanged the following day to regular culture medium ...
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bioRxiv - Cell Biology 2019Quote: Osteoclasts were transfected with a PGK HRE–firefly luciferase plasmid (gifted by Professor AL Harris, Oxford, UK) and a pHRG–TK Renilla luciferase control plasmid (Promega, Southampton, UK) using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Systems Biology 2019Quote: ... Luciferase assays were performed in CH12 cells by co-transfecting 10 μg of with test plasmid and 0.5 μg of TK-Renilla luciferase reporter plasmid (Promega Catalog No. E6931) as control ...
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bioRxiv - Neuroscience 2019Quote: ... HEK293T cells were transfected with control (mCherry) or Cre-T2A-mCherry-containing pFUGW vector plasmids and helper plasmids PAX2 and VSVG using Fugene6 (Promega, Cat #E2691). Supernatant was collected 65 hr after transfection and filtered through a 0.45 μm filter (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... Halo-EGFR constructs used for FRET-FLIM experiments were created using the HA-EGFR plasmid and the Gibson assembly approach to replace the N-terminal HA tag with the HaloTag® plasmid (Promega, #G7711). The final resulting construct contained the HaloTag and a GSGS linker region after the EGFR signal sequence ...
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bioRxiv - Biochemistry 2023Quote: ... GAG/POL and VSVG plasmids were co-transfected with transfer plasmid into HEK293Ts or HEK293FTs using FuGENE HD (Promega, 3.33:1 FuGENE:DNA), or lipofectamine 3000 Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... 6.10^5 S2 cells were transfected with a mix consisting of 100ng of luciferase plasmid and 5ng of Renilla plasmid per well using FuGENE® HD (Promega, #E2311). At least tree biological l replicates were measured per construct ...
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bioRxiv - Developmental Biology 2021Quote: ... and 20ng pRL-TK Renilla control plasmid construct (Promega) using Fugene HD transfection reagent ...
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bioRxiv - Cell Biology 2022Quote: The pGL4.41[luc2P/HSE/Hygro] plasmid (HSE-Luc; Promega) was transfected into HEK293T cells and a stable clone expressing HSE-Luc was selected with hygromycin (200 mg/mL) ...
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bioRxiv - Molecular Biology 2021Quote: ... Renilla luciferase control plasmid (RLuc) was obtained from Promega. λN was synthetized and cloned into pcDNA3.1 (- ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid DNAs were transfected using Fugene HD (Promega #E2312) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2020Quote: The test plasmids used a modified pGL4.12[luc2CP] (Promega) vector backbone where the control of expression of the Firefly ORF was modified by the addition of an upstream CMV promoter ...
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bioRxiv - Cell Biology 2019Quote: ... and co-transfection with a Renilla luciferase plasmid (Promega) served as a transfection control ...
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bioRxiv - Biophysics 2019Quote: ... or PureYield Plasmid Miniprep with Endotoxin removal wash (Promega).
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bioRxiv - Genetics 2020Quote: ... Plasmids were purified with Wizard Plus SV Minipreps (Promega) and sequenced.
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bioRxiv - Genomics 2019Quote: ... and DNA isolated using PureYield Plasmid Midiprep system (Promega). Plasmid DNA was in vitro methylated using CpG Methyltransferase (M.SssI ...
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bioRxiv - Immunology 2021Quote: ... together with a pRL-TK Renilla reporter plasmid (Promega) for normalization of transfection efficiency ...
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bioRxiv - Immunology 2021Quote: ... all plasmid transfections were performed with FuGene HD (Promega) following manufacturers recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids were transfected into HEK293T with ViaFect (#E4982, Promega). 2.5ng of total DNA was added per well in a 6-well plate at reagent:DNA ratio of 4:1 ...
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bioRxiv - Biochemistry 2020Quote: GFP plasmid transfections were conducted using Fugene HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... NanoLuc was PCR amplified from plasmid pNL.1.1 (Promega) and cloned into pUS2-MT to generate pUS2-MT-Nanoluc ...
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bioRxiv - Microbiology 2021Quote: ... Firefly and renilla luciferase reporter plasmids are from Promega.
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bioRxiv - Cancer Biology 2020Quote: ... The pRL-null Renilla plasmid (Promega, Madison, WI, USA) was also transfected ...
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bioRxiv - Cell Biology 2021Quote: ... and 750ng of transfection carrier DNA (Promega, pGEM2 plasmid). Cells were expanded in 10cm plate on day 2 and analyzed by fluorescent flow cytometry on day 3 with an Amnis Imagestream X (Luminex ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid transfection was performed using Fugene HD (Promega E2311) according to the manufacture’s guideline.
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bioRxiv - Cancer Biology 2022Quote: ... plasmids were transfected into cells using Fugene HD (Promega). After 48 h ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was subcloned into plasmid psiCHECK2 (Promega) previously digested with NotI using the In-Fusion cloning kit (Takara Bio ...
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bioRxiv - Microbiology 2021Quote: ... and 0.2 g Renilla plasmid (pRL-CMV, Promega, E2261) at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Transfections of plasmids were performed using Fugene HD (Promega) and that of siRNAs was performed using Lipofectamine RNAi Max (Thermosfisher ...
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bioRxiv - Microbiology 2022Quote: ... The plasmid was transformed into BL21(DE3) pLysS (Promega) by heat shock ...
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bioRxiv - Molecular Biology 2019Quote: ... Transfection of these plasmids was performed using FugeneHD (Promega) at a 1:1:8 ratio of L-TALEN:R-TALEN:Donor as previously described (Lee et al. ...
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bioRxiv - Cancer Biology 2019Quote: ... Reporter plasmid and a Renilla transfection normalization vector (Promega) were used.
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bioRxiv - Molecular Biology 2020Quote: ... The plasmids were transfected into cells using Fugene6 (Promega) or Turbofect (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2020Quote: ... Plasmids were transfected with FuGENE HD Transfection Reagent (Promega).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... an envelope plasmid encoding VSV-G and pAdVAntage (Promega). Four days after transfection ...
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bioRxiv - Molecular Biology 2021Quote: Plasmids were linearized and transfected using Fugene HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... together with 0.1 μg pRSV-β-gal plasmid (Promega) as a control for transfection efficiency ...
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bioRxiv - Molecular Biology 2019Quote: ... the plasmid was recovered using a miniprep kit (Promega), DpnI digested and transformed into MBM7070 indicator bacteria ...
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bioRxiv - Biochemistry 2020Quote: ... Transfections of plasmids were performed using Fugene HD (Promega) at and that of siRNAs was performed using Lipofectamine RNAi Max (Thermosfisher ...
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bioRxiv - Biochemistry 2021Quote: The pBESTluc plasmid was obtained from Promega (No. L1020) and contains the eukaryotic firefly luciferase gene positioned downstream from a Ptac promoter and a ribosome binding site [26] ...
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bioRxiv - Microbiology 2021Quote: ... A MafF expression plasmid (pFN21AB8874) was purchased from Promega. To add a C-terminal HaloTag ...
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bioRxiv - Immunology 2021Quote: ... 5 ng of Renilla luciferase plasmid (Promega pRL-CMV), and 50 ng of Gal4-DNA binding domain-human RORγt-ligand-binding domain fusion protein plasmid per each well ...
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Evolutionary plasticity of SH3 domain binding by Nef proteins of the HIV-1/SIVcpz lentiviral lineagebioRxiv - Microbiology 2021Quote: ... plus 50 ng of the plasmid pRL-TK (Promega) expressing low and constitutive levels of Renilla luciferase ...
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bioRxiv - Immunology 2020Quote: ... plasmid transfection was carried out using FuGENE HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and a SV40-Renilla luciferase plasmid (pRL-SV40, Promega) as a reference were used ...