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Citations for Promega :
601 - 650 of 1480 citations for Human PLA2G4F shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... Plasmid were delivered to cells using Fugene HD Transfection Reagent (Promega #E5912) at a 3:1 Fugene volume:DNA mass ratio for transfection complex formation in Opti-MEM I Reduced Serum Medium (Thermo Fisher Scientific #31985070) ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1 μg of pCMV-VSV-G plasmids using FugeneHD (Promega, E2311) in Opti-MEM (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... The template DNA fragments were PCR-amplified using the psiCHECK2 plasmid (Promega) with 5′-TAATACGACTCACTATAGG-3′ and 5′-CACACAAAAAACCAACACACAG-3′ (Rluc) ...
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bioRxiv - Molecular Biology 2024Quote: ... The template DNA fragments were PCR-amplified using the psiCHECK2 plasmid (Promega) with 5′-TAATACGACTCACTATAGG-3′ and 5′-CACACAAAAAACCAACACACAG-3′ (Rluc) ...
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bioRxiv - Molecular Biology 2019Quote: ... and human 293T cells as producer cells using the FUGENE HD transfection reagent (Promega), as described [50] ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... we used a 1/1500 artificial mix of promastigote DNA and human DNA (Promega) to reflect the median ratio found in clinical samples ...
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bioRxiv - Neuroscience 2022Quote: Open reading frames of human OR genes were subcloned into pCI (Promega, WI, USA) with a Rho-tag (the sequence encoding the first 20 amino acids of rhodopsin ...
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bioRxiv - Immunology 2023Quote: ... tailed with complete TRAC and TRBC1 gene segments amplified from human genomic DNA (Promega), respectively ...
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bioRxiv - Biochemistry 2024Quote: ... 24 hours after transient transfection with mouse or human ENPP3 via Fugene 6 (Promega), the media was gently removed and replaced with serum-free DMEM supplemented with 1% insulin-transferrin-selenium-sodium pyruvate (ThermoFisher ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μg of plasmid was transfected using Fugene HD (Promega, Madison WI, #E2311). Plasmid DNA was mixed with 8 µL Fugene and 100 µL media and incubated at room temperature for 15 minutes before being added to cells ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid DNA and siRNA transfections were performed using FuGENE 6 Transfection Reagent (Promega) and Lipofectamine RNAiMAX (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with the appropriate plasmid using the FuGeneHD transfection reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Plasmid transfection of HEK293F and HeLa cells was performed with Fugene HD (Promega), whereas SKBr3 cells were transfected using Fugene 6 (Promega) ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid transfection was performed with Jet PEI (PolyPlus Transfection) or Fugene HD (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... internal control plasmid pRL-TK (0.02 μg/well; Promega Corp.; Fitchburg, WI, USA), and the indicated expression constructs ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were transfected with the appropriate plasmids using Fugene HD Transfection Reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified twice with a miniprep kit (PureYield™ Plasmid Miniprep System, Promega). Plasmids were eluted with RNase free water and used as templates for in vitro gene expression (E ...
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bioRxiv - Evolutionary Biology 2019Quote: Plasmid DNA was isolated using the Wizard Plus Minipreps DNA purification systems (Promega). Sequencing of the SHV gene was performed using M13 primers (MCLab ...
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Mutant ACTB mRNA 3′UTR Promotes Hepatocellular Carcinoma Development by Regulating miR-1 and miR-29abioRxiv - Cancer Biology 2019Quote: ... The PCR product was cloned into the pGL3-promoter luciferase-based plasmid (Promega) at the cloning site between KpnI and XhoI ...
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bioRxiv - Immunology 2019Quote: ... Plasmids were transfected into RAW264.7 cells with FuGENE HD (Promega, Madison, WI, USA). At 50 % confluency ...
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bioRxiv - Microbiology 2019Quote: ... DNA restriction nucleases were used to digest plasmids (Promega and New England Biolabs). Gibson assembly mixes were used according to manufacturer instructions to construct the plasmids used in this study (New England Biolabs) ...
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bioRxiv - Physiology 2019Quote: ... Transfection of plasmids or siRNAs was performed with FuGene HD (Promega, Madison, WI) or Lipofectamine RNAimax (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... ISRE-firefly luciferase and pTK-renilla luciferase (pRL-TK) plasmids were from Promega. Vectors expressing MAVS ...
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bioRxiv - Biochemistry 2020Quote: A549 cells were transiently transfected with the plasmid pGL4.37[luc2P/ARE/Hygro] (Promega) and selected with 500 μg/ml of hygromycin to obtain a stable cell line harboring the ARE-luc2P reporter ...
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bioRxiv - Cell Biology 2021Quote: DNA plasmid transfections were performed using Fugene HD transfection reagent (Promega Corporation, E2311) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... 100 ng/well indicated plasmids and 10 ng pRLTK internal control vector (Promega) were co-transfected with 1.25 μl Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... These fusions were expressed from plasmids based on the pGEM-T backbone (Promega) named pOP plasmids (22) ...
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bioRxiv - Cancer Biology 2020Quote: ... The plasmids were diluted in Opti-MEM and Fugene HD transfection reagent (Promega). The DNA-mixture was incubated for 30 min and then added drop-wise to the cells cultured in DMEM without penicillin/streptomycin for 6-7 h ...
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bioRxiv - Cancer Biology 2020Quote: ... The plasmids were diluted in Opti-MEM and Fugene HD transfection reagent (Promega). The DNA-mixture was incubated for 30 min and then added drop-wise to the cells cultured in DMEM without penicillin/streptomycin for 6-7 h ...
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bioRxiv - Cancer Biology 2022Quote: ... and a Renilla luciferase reporter plasmid driven by a constitutive CMV promoter (Promega). 24 hours later ...
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bioRxiv - Microbiology 2022Quote: Plasmids (Table S2) used were constructed with DNA restriction nucleases (Promega – WI, USA), Gibson Assembly mix (New England Biolabs – MA ...
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bioRxiv - Immunology 2022Quote: ... Plasmids were transfected into cells with the aid of FugeneHD (Promega, Cat. E2311) using a ratio of 4 uL FugeneHD per 1 ug of plasmid DNA ...
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bioRxiv - Cell Biology 2022Quote: Transfection of plasmid DNA in HeLa cells was carried out using FuGENE (Promega). For the DNA transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... Plasmid purifications were performed utilising Wizard Plus SV Minipreps DNA Purification Systems (Promega). New plasmids were tested by restriction enzyme digests and sequencing (Eurofins/GATC or Microsynth) ...
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bioRxiv - Microbiology 2020Quote: ... Fluc was amplified from pGL4.32[luc2P NF-kB-RE] plasmid (Promega, Madison, WI) and inserted into the vector backbone using Gibson Assembly ...
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bioRxiv - Genetics 2022Quote: ... HEK cells were transfected with 500 ng of plasmid using FuGENE 6 (Promega) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2019Quote: DNA plasmid transfection were performed as previously described using FugeneHD (Promega, Madison, WI) 51 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... This genomic region had previously been cloned into the pGEM-T plasmid (Promega) and transformed into Escherichia coli(Ye et al ...
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bioRxiv - Neuroscience 2019Quote: ... and pGlosensorTM -22F cyclic adenosine monophosphate (cAMP) biosensor plasmid (Promega Corp., Madison, WI), which were used for receptor activation and intracellular signalling assays.
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bioRxiv - Molecular Biology 2019Quote: ... Plasmid DNA (6 μg) was delivered into U2OS cells using FuGENE 6 (Promega) and 2.5 μg of plasmid DNA was delivered into HeLa cells using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The coding sequence of BclA3CTD was cloned in the plasmid pGEMT-easy (Promega) and posteriorly cleaved with BamHI/PstI restriction enzymes and inserted in-frame to an N-terminal polyhistidine tag in the expression vector pRSETA (Invitrogen) ...
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bioRxiv - Genetics 2020Quote: Plasmids were transfected into U2OS cells using FuGENE® HD Transfection Reagent (Promega), according to the manufacture’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Oligos were annealed and inserted into the NotI/XhoI-digested psiCHECK2 plasmid (Promega). 1E5 cells/well were plated in 48-well plates and transfected with empty ...
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bioRxiv - Genomics 2019Quote: Linearized plasmid (2.5 ug) was added to a mixture containing transcription buffer (Promega), 10 mM DTT ...
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bioRxiv - Biophysics 2019Quote: ... The plasmids were transformed in Rosetta (DE3) and Rosetta (DE3) pLys cells (Promega). Cells were grown to an optical density of 0.7-0.8 in Luria-Bertani (LB ...
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bioRxiv - Genetics 2021Quote: ... Plasmid and template were co-transfected into LF2 mESCs using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... 0.02 ug of the pRL-CMV Renilla Luciferase plasmid (Promega Accession No. AF025843) was co-transfected with firefly luciferase plasmids ...
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bioRxiv - Developmental Biology 2021Quote: ... Individual colonies were selected and isolated using the PureYield Plasmid Miniprep System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... where Firefly luciferase was substituted in the plasmid for NanoLuc luciferase (Promega, USA), transfected to HEK293 cells and IFNα-luciferase fusion proteins collected with tissue culture supernatant ...
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bioRxiv - Genomics 2019Quote: ... 400ng of the construct was transfected together with 40ng of Renilla plasmid (Promega) using lipofectamine 2000 (Thermo Fisher Scientific) ...