Labshake search
Citations for Promega :
151 - 200 of 6245 citations for Human H ACA Ribonucleoprotein Complex Subunit 1 GAR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2020Quote: ... the membrane was incubated for 1 h with a horseradish peroxidase (HPR) conjugated anti-rabbit antibody (Promega, Walldorf, Germany) diluted in PBS-T with 3% milk powder ...
-
bioRxiv - Molecular Biology 2022Quote: ... The proteins were digested for 2 h at RT using 0.5 μg Lys-C (Wako Chemicals) and for 16 h at 37 °C using 1 μg trypsin (Promega). After centrifugation (10 min at 14 000 g) ...
-
bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated for 1 h with a horseradish peroxidase (HPR) conjugated anti-rabbit antibody (Promega, Walldorf, Germany) diluted in PBS-T with 3% milk powder ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were proteolysed for 2 h at room temperature using 0.5 μg Lys-C (Wako) and subsequently for 16 h at 37 °C using 1 μg trypsin (Promega). Peptides were collected by centrifugation and acidified with 0.5 % trifluoroacetic acid.
-
bioRxiv - Biochemistry 2023Quote: ... Beads were resuspended in 500 µL of 100 mM Tris-HCl pH 8.0 containing 2 mM CaCl2 and digested at 37 °C for 1 h with 0.2 μg of trypsin/LysC (#V5073 Promega). The samples were then loaded onto homemade SepPak C18 Tips packed by stacking three AttractSPE disk (#SPE-Disks-Bio-C18–100.47.20 ...
-
bioRxiv - Immunology 2023Quote: ... the cells plus supernatants were combined with an equal volume of the respective caspase-Glo reagent for 1 h according to the manufacturer’s instructions (Promega). The mixture was then transferred to a Lumitrac 600 plate and luminescence was measured using a SpectraMax i3 instrument and the SoftMax Pro 6.3 Software (Molecular Devices) ...
-
bioRxiv - Developmental Biology 2020Quote: ... Digests were carried out in 4M urea 100mM HEPES with LysC (Wako, #121-05063, 1/100 (w/w) protease/substrates) for 3 h at 37C and subsequent trypsin digest (Promega, #V5280, 1/100 (w/w) protease/substrates ...
-
bioRxiv - Biochemistry 2019Quote: ... Media was replaced at 24 h post-transfection and cells were lysed at 48 h post-transfection using 200 ml passive lysis buffer (Promega) followed by freezing at −80 °C ...
-
bioRxiv - Biochemistry 2019Quote: ... Media was replaced at 24 h post-transfection and cells were lysed at 48 h post-transfection using 200 ml passive lysis buffer (Promega). FLuc and RLuc activity was measured using a dual-luciferase reporter assay system (Promega) ...
-
bioRxiv - Microbiology 2019Quote: ... Proteins were digested for 2 h at 37°C with Lys-C and 16 h at 37°C with Trypsin Gold (both Promega). For TMT 6-plex labeling (Thermo Fisher Scientific) ...
-
bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
-
bioRxiv - Neuroscience 2020Quote: ... All designed probes were tested by ddPCR using human genomic DNA (Human male, Promega) as a negative control ...
-
bioRxiv - Systems Biology 2023Quote: Methyltransferase activity of the NSP10-NSP16 complex and inhibitor screening was performed using the MTase-Glo assay (Cat. No. V7601, Promega) (Hsiao et al ...
-
bioRxiv - Genomics 2020Quote: ... and M-MLV Reverse Transcriptase RNase H (Promega). cDNA was amplified using Taqman Universal Master Mix with UNG (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2019Quote: ... the samples were treated with RNase H (Promega) for 30 min at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... Anti-rabbit IgG (H+L) HRP Conjugate (Promega) was used as the secondary antibody ...
-
bioRxiv - Biochemistry 2021Quote: ... reverse transcribed using MMLV reverse transcriptase (H-)(Promega) at 42°C for 1 hour and buffer exchanged to TBS-T using sephadex G-25 ...
-
bioRxiv - Microbiology 2022Quote: ... using MMLV reverse transcriptase RNase H Minus (Promega). Purified resuspended cDNAs were sequenced on a CEQ 8000 capillary electrophoresis sequencer (9+) ...
-
bioRxiv - Cell Biology 2021Quote: ... and Anti-Rabbit IgG (H+L) Conjugate (Promega).
-
bioRxiv - Genetics 2022Quote: Endoglycosidase H (EndoH) (Promega V4871, 500 U/μL) was used to deglycosylate Ina1-GFP-containing samples ...
-
bioRxiv - Biochemistry 2022Quote: ... the proteins were deglycosylated by Endo-H (Promega) followed by PNGaseF (Promega ...
-
bioRxiv - Neuroscience 2023Quote: ... cells were incubated with 10µM Coelenterazine-h (Promega) in the dark for 2 hours before recording luminescence in a plate reader (Optima FLUOStar ...
-
bioRxiv - Cancer Biology 2023Quote: Total RNA from MB cells treated with RA for 48 h was extracted using the SV Total RNA Isolation System kit (Promega, Madison, USA), following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... The cells were lysed 24 h after transfection and luciferase activity was measured with the Dual-Luciferase Reporter Assay kit (Promega Cat. # E1960) according to the manufacturer’s protocol using a FlUOstar plate reader (BMG Labtech) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293T cells were co-transfected in a 1:1 ratio with either human D1 or D2Long receptor and a split-luciferase based cAMP biosensor (GloSensor, Promega, Durham NC). The next day ...
-
bioRxiv - Molecular Biology 2022Quote: ... Human Genomic DNA (G1521, Promega) was used as positive control and synthetic DNA was used to validate the LAMP assay performance ...
-
bioRxiv - Microbiology 2020Quote: ... GoTaq 1-Step RT-qPCR kit (Promega) was used at a final volume of 20 μl with ...
-
bioRxiv - Molecular Biology 2020Quote: ... followed by the addition of 5-μL Endo-H reaction buffer and 5-μL Endo-H (Promega Cat#PRV4875, 2,500-U). Deglycosylation proceeded for 4-hours at 37°C ...
-
bioRxiv - Microbiology 2019Quote: ... Relative growth was determined using luciferase levels measured at 0 h (initial setup values) and after 72 h using the GloMax® Discover Multimode Microplate Reader (Promega). Luminescence values were normalized to samples treated with a lethal dose of chloroquine (200 nM ...
-
bioRxiv - Developmental Biology 2019Quote: ... 96 and 120 h after transfection and 4 h after adding 20 µl of CellTiter 96® AQ One Solution Reagent (Promega) to each well containing transfected TCam-2 cells.
-
bioRxiv - Microbiology 2020Quote: ... Cells were treated 8 h post-transfection with or without IFN-α (1000 U/ml) before lysis 16 h later using Passive Lysis Buffer (Promega). Firefly and Renilla luciferase activity was then determined in a dual luciferase assay ...
-
bioRxiv - Microbiology 2022Quote: ... The samples were incubated at 30 °C and luminescence was quantified after 2 h and 4 h post infection (20 μl sample plus 20 μl substrate, Nano-Glo® Luciferase Assay System, Promega). The sample was carefully removed from the upper part of the tube without disturbing the sedimented erythrocytes in the blood samples.
-
bioRxiv - Microbiology 2023Quote: ... The presence of glycosylated proteins in the cell lysates was assessed using Promega Endoglycosidase H (Endo H) assay reagents (Promega V4871). Briefly ...
-
bioRxiv - Microbiology 2022Quote: ... washed three times (10 minutes each) with PBST before incubation for 1 h with α-Mouse IgG HRP Conjugate (Promega) secondary antibody (1:10 000 in PBST) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Samples were subjected to digestion of Lys-C (Wako Chemicals) (1:50) for 2 h at RT and then to trypsin (Promega) (1:50 ...
-
bioRxiv - Cell Biology 2022Quote: ... two micrograms of RNA from each sample were reverse transcribed at 42 °C for 1 h followed by 95 °C for 10 min M-MLV Reverse Transcriptase (Promega) and oligo(T)18 primers (Takara) ...
-
bioRxiv - Genomics 2020Quote: ... A double-stranded DNA adapter with a T-overhang was ligated for 1 h at 20°C and the resulting products were bead purified (ProNex, Promega), eluted and then split into two identical aliquots ...
-
bioRxiv - Molecular Biology 2019Quote: ... 1 μl of 10 mM ddTTP for the sequencing reaction and 1 μl of MMLV Reverse Transcriptase RNAse H minus (Promega) were finally added and the reverse transcription was performed at 35°C for 2 minutes ...
-
bioRxiv - Systems Biology 2019Quote: ... The sample was digested for 16 h at 37C using a 1:50 (w/w) ratio of Sequencing Grade Modified Trypsin (Promega):protein in the gel ...
-
bioRxiv - Biochemistry 2019Quote: ... The washed beads were resuspended in 200 µl of digestion buffer and incubated for 5 h with 1 µg of trypsin (Promega) at 37°C ...
-
bioRxiv - Neuroscience 2021Quote: ... The messenger RNAs (mRNAs) contained in 1 μg of purified RNA extracts were reverse-transcribed using the reverse transcriptase RNase H minus (Promega) and oligo-d(T)15 ...
-
bioRxiv - Plant Biology 2021Quote: ... 1 μg of RNA was converted into cDNA with M-MLV RNase H–Point Mutant reverse transcriptase (Promega Corp., USA) and an anchored oligo dT21 primer (Metabion ...
-
bioRxiv - Plant Biology 2020Quote: ... and then reverse-transcribed with a cox1 specific primer (Atcox1-1; table S1) using M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RNase H minus, point mutant; Promega). The RNA template was degraded by adding 1/5 volume of 1 M NaOH to the reaction mixture and incubating at room temperature for 10 minutes ...
-
bioRxiv - Plant Biology 2020Quote: ... The soluble fraction was then digested for 20 h at 37°C using a 1:50 ratio of trypsin (Promega) to extracted protein while gently shaking ...
-
bioRxiv - Biochemistry 2022Quote: ... Samples were diluted with ddH2O 1:5 and proteins were digested for 20 h at 37°C using trypsin (Trypsin Gold, Promega) at an enzyme/protein ratio of 1:50 ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... the proteins were alkylated with iodoacetamide (100 mM for 1 h at room temperature in a dark chamber) and digested with trypsin (Promega) diluted in ammonium bicarbonate (0.1 M for 24 h at 37 °C) ...
-
bioRxiv - Microbiology 2020Quote: ... Protein digestion was performed using 1:100 (w/w) LysC (Wako Chemicals) for 4 h at 37°C and then finalised with 1:50 (w/w) trypsin (Promega) overnight at 37°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... Cells were harvested 36 h after transfection by lysing in 100 μl of 1× reporter lysis buffer (Promega, Madison, WI). Lysates were pelleted ...
-
bioRxiv - Developmental Biology 2021Quote: ... Samples were washed twice with UA buffer and twice with 50mM ammonium bicarbonate prior to digest of the immobilized proteins on the filter for 2 h at RT using 1 mg Lys-C (Wako Chemicals) and for 16 h at 37C using 2 mg trypsin (Promega). Tryptic peptides were collected by centrifugation (10 min at 14,000 g) ...
-
bioRxiv - Biophysics 2021Quote: ... were plated into six-well tissue culture plates containing collagen-coated glass cover slips 12 to 24 h before being transfected with 1 μg of the indicated plasmid DNA using FuGENE 6 transfection reagent (Promega) following the manufacturer’s protocol ...