Labshake search
Citations for Promega :
101 - 150 of 180 citations for Glutathione S transferase Class mu 26 kDa Isozyme GST Schistosoma Japonicum since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2020Quote: HALO-FDL1 and HALO-GST (negative control) DAP-seq samples were performed using protein expressed in the TNT rabbit reticulocyte expression system (Promega) as previously described (Bartlett et al. ...
-
bioRxiv - Biochemistry 2019Quote: ... Dimeric constructs are based on VY208 and were created by artificially dimerization through an N-terminal GST-tag (Reck-Peterson et al, 2006) and tagged with a HaloTag (Promega) at the C-terminus as well as a GFP at the very N-terminus ...
-
bioRxiv - Plant Biology 2021Quote: ... The GST-PIF3 and GST-PIF3mTAD constructs for GST pulldown assays were generated by subcloning PIF3 or PIF3mTAD into the EcoRI/XhoI sites of the pET42b vector (Promega). The PIF3p::HA-YFP-PIF3 and PIF3p::HA-YFP-PIF3mTAD constructs were generated by subcloning 2-kb PIF3 promoter ...
-
bioRxiv - Systems Biology 2020Quote: ... + P/S + 0.5μg/mL Puromycin + 16ng/mL rhFGF-basic (Promega G5071).
-
bioRxiv - Genetics 2021Quote: ... allowing us to screen F1’s by PCR (Promega GoTaq mastermix) followed by restriction digests with AflII (NEB) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Luminescence was measured every 30 s using the GloMax Explorer (Promega).
-
bioRxiv - Neuroscience 2020Quote: The ratio of reduced (GSH) to oxidised (GSSG) glutathione was determined using a commercial assay (GSH:GSSG-Glo assay, Promega Co, Southampton, UK) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: Cellular ROS was measured through the detection glutathione in its reduced (GSH) and oxidized (GSSG) forms using the luminescence-based GSH/GSSG-Glo™ Assay (Promega, V6611). Briefly ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 30 s at 68°C or through GO taq polymerase (Promega) for 2 min at 95°C followed by 25 cycles of 30 s at 95°C ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... using 0.3 s integration time according to the “NanoBRET 618” protocol (Promega).
-
bioRxiv - Systems Biology 2023Quote: ... + P/S + 0.5ug/mL puromycin + 16 ng/mL rhFGF-basic (Promega #G5071). When passaging hiF-T cells ...
-
bioRxiv - Neuroscience 2023Quote: ... the 130-kDa band was cut out as a gel piece and digested with trypsin (Promega) at 37°C overnight ...
-
bioRxiv - Biophysics 2022Quote: ... transient transfection of the appropriate plasmid(s) was performed using FuGENE HD (Promega) according to the manufacturer’s procedure ...
-
bioRxiv - Immunology 2023Quote: ... and applied to S-Traps (mini; Protifi) for tryptic digestion (sequencing grade; Promega) in 50 mM TEAB ...
-
bioRxiv - Immunology 2024Quote: ... and applied to S-Traps (mini; Protifi) for tryptic digestion (sequencing grade; Promega) in 50 mM TEAB ...
-
bioRxiv - Plant Biology 2021Quote: The cDNAs corresponding to the two alleles of SoIAA2 were cloned into a pFN2A (GST) flexi vector (Promega – primers in Supplementary Table 1), expressed in E ...
-
bioRxiv - Molecular Biology 2023Quote: ... Bacterial lysates expressing fused GST-CuLCrV / SiGMV / TYLCV CP proteins and GST control were incubated with MagneGST□ particles (cat#V8600, MagneGST protein purification system, Promega, Madison, WI, USA) to immobilize the GST tagged proteins as per manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... Luciferase intensity was measured with a GloMax luminometer (10-s signal integration; Promega BioSystems).
-
bioRxiv - Molecular Biology 2021Quote: The effect of menadione on the induction of oxidative stress was determined by measuring the ratio between oxidized and reduced glutathione (GSH/GSSG) using a commercial kit by Promega (GSH/GSSG-Glo Assay, V6611).
-
bioRxiv - Microbiology 2022Quote: ... and SV40 late poly(A) signal was amplified from pNL1.1.CMV[Nluc/CMV] (19.1 kDa NanoLuc protein, Promega). The PCR product was blunt end ligated to pAD1 digested with EcoRV ...
-
bioRxiv - Biochemistry 2023Quote: ... The methyltransferase reaction product S-adenosylhomocysteine was quantified using MTase-Glo kit (Promega, Wisconsin, USA) following the manufacturer’s protocol (43) ...
-
bioRxiv - Cell Biology 2023Quote: ... and applied to S-Traps (micro; Protifi) for tryptic digestion (sequencing grade; Promega, Madison, WI) in 50 mM TEAB ...
-
bioRxiv - Bioengineering 2024Quote: ... The Glucose-Glo and Lactate-Glo kits (Cat #’s J6021 and J5021, Promega, Madison, WI) were used to measure the apparent glucose and lactate concentrations in media samples according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2020Quote: ... pH 8.0 using Vivaspin columns (3 kDa) and digested separately O/N using trypsin or chymotrypsin (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...
-
bioRxiv - Biochemistry 2019Quote: ... and the band corresponding to ~64 kDa was incised and in-gel digested by trypsin according to the manufacturer’s protocol (Promega). Peptides were then extracted from the gel and analyzed by LC-MS using an Eksigent nano-HPLC (model nanoLC-2D ...
-
bioRxiv - Microbiology 2020Quote: ... pH 8.0 using Vivaspin columns (3 kDa) and digested separately overnight using trypsin or chymotrypsin (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...
-
MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... Sigma-Aldrich)–coated dishes or glass coverslips with 30 ng/ml of 2.5 S mouse NGF (G5141, Promega) in the Neurobasal medium containing B27 supplement (17504044 ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... S-Traps were loaded with 125 μL trypsin solution (50 ng/μL in 50mM TEAB; Promega) and incubated at 47°C for 2 hours ...
-
bioRxiv - Biochemistry 2022Quote: A large gel piece covering a wide MW range above 100 kDa was cut out and subjected to reduction/alkylation and in-gel digestion with trypsin (Promega) as previously described [19b] ...
-
bioRxiv - Immunology 2022Quote: ... pH 8.0 using Vivaspin columns with a 3 kDa molecular weight cutoff and digested separately overnight using trypsin chymotrypsin or Glu-C (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...
-
bioRxiv - Microbiology 2020Quote: ... pH 8.0 using Vivaspin columns (3 kDa) and digested separately overnight using trypsin chymotrypsin or alpha lytic protease (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...
-
bioRxiv - Immunology 2021Quote: ... pH 8.0 using Vivaspin columns (3 kDa) and two of the aliquots were digested separately overnight using chymotrypsin (Mass Spectrometry Grade, Promega) or alpha lytic protease (Sigma Aldrich ...
-
bioRxiv - Immunology 2022Quote: ... pH 8.0 using Vivaspin columns (3 kDa) and two of the aliquots were digested separately overnight using chymotrypsin (Mass Spectrometry Grade, Promega) or alpha lytic protease (Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2022Quote: ... pH 8.0 using Vivaspin columns (3 kDa) and two of the aliquots were digested separately overnight using trypsin (Mass Spectrometry grade, Promega), chymotrypsin (Mass Spectrometry Grade ...
-
bioRxiv - Microbiology 2021Quote: ... At 1 × s after injection of 20 µl of the substrate solution (Renilla Luciferase Assay System, Promega), relative light units (RLUs ...
-
bioRxiv - Cell Biology 2020Quote: KNL-2-MBP fragments were [35]S labeled using TnT® Quick Coupled Transcription/Translation System (Promega). 10 μl of the in vitro translation lysate was incubated for 1hr at 4°C with 50 μg CENP-AHCP-3(1-109)-MBP-His (in 20 mM Tris [pH7.5] ...
-
bioRxiv - Immunology 2023Quote: ... VP1-3 proteins were then bound to the S-Trap column and digested with trypsin (Promega Corporation) for 2 hrs at 47°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... The Nluc and BRET signals were measured (integration time: 1 s) after auto-injecting either Fz (Promega) or FFz (Promega ...
-
bioRxiv - Immunology 2024Quote: ... FC tags on the S and RBD proteins were removed through treatment with Factor Xa Proteinase (Promega).
-
bioRxiv - Cell Biology 2022Quote: ... The beads were collected by centrifugation at 8000 × g for 30 s or using a magnet separator (Promega), and washed three times with lysis buffer ...
-
bioRxiv - Immunology 2022Quote: The ADCC activity of anti-SARS-CoV2 S IgG antibodies was determined using the ADCC Reporter Bioassay (Promega) as previously described (Dufloo et al. ...
-
bioRxiv - Microbiology 2020Quote: ... Donor cells were co-transfected with 1.5 μg pCAGGS-S and 0,5 μg pmCherry-N1 using 6 μl of Fugene 6 following the manufacturer’s instructions (Promega). Acceptor cells were treated with CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate ...
-
ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... purified protein samples were prepared by S-alkylation with iodoacetamide and digestion with either with LysC/GluC (Promega) [RBD] or ...
-
bioRxiv - Systems Biology 2021Quote: ... alkylated in the dark with iodoacetamide and applied to S-Traps (micro; Protifi) for tryptic digestion (sequencing grade; Promega) in 50 mM TEAB ...
-
bioRxiv - Cancer Biology 2020Quote: ... alkylated in the dark with iodoacetamide and applied to S-Traps (mini; Protifi) for tryptic digestion (sequencing grade; Promega) in 50 mM TEAB ...
-
bioRxiv - Cancer Biology 2022Quote: ... All cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM) (HyClone) with 10% fetal bovine serum FBS (HyClone) and 1% penicillin-streptomycin (P.S.) (Promega) at 37 °C in a 5% CO2 atmosphere.
-
bioRxiv - Microbiology 2022Quote: ... either with SARS-CoV-2 S and Jun or with ACE2 and Fos plus the pRL Renilla Luciferase (Promega) to normalize the signal ...
-
bioRxiv - Microbiology 2023Quote: ... Sequences of all plasmids were verified using a SupreDye v3.1 Cycle Sequencing Kit (M&S TechnoSystems, Cat# 063001) with a Spectrum Compact CE System (Promega). The psPAX2-IN/HiBiT plasmid was a kind gift from Dr ...
-
bioRxiv - Biochemistry 2023Quote: ... Enzymatic digestion and detergent removal were performed using the S-Trap device and Trypsin Gold (Promega, Madison, Wisconsin, USA) following the manufactures recommended protocol ...
-
bioRxiv - Biochemistry 2023Quote: ... samples were washed twice with S-Trap buffer before overnight digestion at 37° C with Trypsin (Promega Cat# V511X) in digestion buffer (50 mM ammonium carbonate ...