Labshake search
Citations for Promega :
1 - 50 of 180 citations for Glutathione S transferase Class mu 26 kDa Isozyme GST Schistosoma Japonicum since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... Human NOCT (1-431) or Schistosoma japonicum GST coding sequences were inserted into pFC3F using the Flexi Cloning System (Promega). To generate NOCT Δ(2-15)-3F ...
-
bioRxiv - Cancer Biology 2022Quote: GSH/GSSG measurement was performed by a glutathione S-transferase (GST) enzyme coupled reaction using (GSH/GSSG Glo kit, Promega). Cells (2×104 ...
-
bioRxiv - Microbiology 2022Quote: Flag-Trim69 was cloned downstream of a gluthathione-S-transferase (GST) in the pTKPL vector (an in-house derivative of the vector pGEXTK, Promega) and purified from bacterial lysates on glutathione agarose beads (Sigma ...
-
bioRxiv - Cell Biology 2023Quote: HaloTag-GST (61 kDa) (Promega, G4491) was labelled with JF646 for 4 hours at 4 ° C on ice with 3x excess HaloTag ligand (buffer ...
-
bioRxiv - Biochemistry 2021Quote: ... GST-4xSUMO2 was purified using MagneGST Glutathione particles (Promega) as previously described150 ...
-
bioRxiv - Cell Biology 2020Quote: ... GST fusion proteins were isolated by binding to glutathione magnetic beads (MagneGST™ Glutathione Particles, Promega) for 30 min at room temperature on a rotating wheel ...
-
bioRxiv - Cell Biology 2024Quote: ... and GST-C-WRN were affinity-purified using glutathione (GSH)-magnetic beads (Promega).
-
bioRxiv - Cell Biology 2023Quote: ... and GST-N-77 WRN were affinity-purified using glutathione (GSH)-magnetic beads (Promega). Fragment purification levels were assessed by SDS-PAGE followed by Coomassie staining.
-
bioRxiv - Molecular Biology 2024Quote: ... GST-tagged AtCFI59 was purified using agarose beads (MagneGSTTM Glutathione Particles; Promega, Madison, USA) and retained on the beads to pull-down purified HIS-tagged AtCFI25a protein in TBS buffer (50 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Plant Biology 2022Quote: ... and GST- GBF3 were purified from 4 ml culture and immobilized on MagneGST Glutathione particles (Promega). Briefly ...
-
bioRxiv - Biophysics 2019Quote: ... Proteasomes were affinity purified with the bait protein GST-Ubl and magnetic beads (MagneGST™ Glutathione Particles, Promega) and eluted with purification buffer containing 25 mM reduced L-glutathione ...
-
bioRxiv - Biochemistry 2021Quote: Pulldowns with cellular extracts were carried out as described for GFP-immunoprecipitations using 1-2 μg of GST-fused proteins bound to glutathione magnetic beads (Promega). For immunoprecipitations of recombinant proteins ...
-
bioRxiv - Plant Biology 2020Quote: ... GST or GST-OsDMI3 immobilized on the Magnet GST Particles (Promega) were incubated with His-OsMKK1/OsMKK6 in GST binding buffer (2 mM KH2PO4 ...
-
bioRxiv - Neuroscience 2023Quote: ... by a terminal Deoxynucleotidyl transferase (Promega, M1875) with a specificity of 2.5 x 106 cpm/ml (C-Fos ...
-
bioRxiv - Cell Biology 2021Quote: ... and 30 units of terminal deoxynucleotidyl transferase (Promega) in 20 μl of transferase buffer at 37°C for one hour The reaction was halted by incubation at 70°C for 10 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... Glutathione release was measured using the GSH-Glo glutathione assay (Promega; V6912), as per the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: The glutathione levels were measured using the GSH-Glo Glutathione Assay (Promega) with 1 mM paracetamol used as a positive control ...
-
bioRxiv - Developmental Biology 2021Quote: ... the GST tag was cleaved from GST-TES-1 using ProTEV Plus (Promega), according to manufacturer’s instructions.
-
bioRxiv - Immunology 2023Quote: ... intracellular glutathione levels were measured with the GSH-Glo™ Glutathione Assay kit (Promega), following the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2022Quote: MagneGST™ Glutathione Particles (Promega) were used for the assay ...
-
bioRxiv - Genetics 2021Quote: Terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) staining (Promega) was used to visualize fragmented gDNA within paraffin-embedded RV tissue sections ...
-
bioRxiv - Biochemistry 2023Quote: ... recombinant GST-tagged MARK2 (Promega) was incubated with tau at 30 °C at 1:100 ratio of MARK2:tau in phosphorylation buffer (25 mM PIPES ...
-
bioRxiv - Microbiology 2023Quote: ... [26]) and PCR chemicals by PROMEGA© with the following PCR conditions ...
-
bioRxiv - Plant Biology 2022Quote: Glutathione and Ascorbic acid content were quantified using the GSH-GLO Glutathione Assay Kit (Promega, Madison, USA) and Megazyme kit (K-ASCO 04/19 ...
-
bioRxiv - Plant Biology 2019Quote: ... and the resulting Mu-STOP amplicon was cloned into pGEM-T Easy (Promega). Mu-STOP transposon was then released from pGEM::Mu-STOP by BglII digestion and purified by GeneJET Gel Extraction Kit (Thermo Fisher) ...
-
bioRxiv - Cell Biology 2022Quote: ... terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL, Promega, Madison, WI, USA), immunohistochemistry (IHC) ...
-
bioRxiv - Biochemistry 2022Quote: Intracellular reduced and total glutathione levels were quantified using the bioluminescent Promega GSH-Glo™ glutathione assay (Promega), as recommended by the supplier ...
-
bioRxiv - Cancer Biology 2023Quote: Glutathione estimation was performed according to the manufacturer’s instructions using GSH-Glo™ Glutathione Assay kit (Promega, USA). Briefly ...
-
bioRxiv - Molecular Biology 2022Quote: ... the GST region of psfRps29-GST was replaced with luciferase cDNA (pNL1.1[Nluc]Vector; Promega Madison, WI, USA). Luciferase cDNA was amplified via PCR using primers Nluc_FW2 and Nluc_RV2 ...
-
bioRxiv - Cell Biology 2020Quote: ... 1μg of total RNA was reverse transcribed using Mu-MLV reverse transcriptase (Promega, Madison, WI). The gene expression was evaluated by Quantitative Real-Time PCR (RT-PCR ...
-
bioRxiv - Plant Biology 2022Quote: ... and MagneGST™ Glutathione Particles (Promega Cat# V861A) following the manufacturer’s manual ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells grown on 10cm culture dishes were transiently transfected with the different HA-GST-RagC mutants and wildtype HA-GST-RagA using Fugene HD (#E2311, Promega). The following day ...
-
bioRxiv - Microbiology 2023Quote: 1 ml of recombinant GST or GST-YgfB protein at a concentration of 10 μM was incubated with 100 μl 50 % MagneGST (Promega) bead-slurry equilibrated with pulldown-buffer for 45 min at 4°C and washed two times with 500 μl pulldown-buffer ...
-
bioRxiv - Plant Biology 2023Quote: ... EIL1-GST and GST proteins were synthesized using the TnT SP6 High-Yield Wheat Germ Protein Expression System (Promega, L3260), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... using the DeadEnd Colorimetric TUNEL (Terminal transferase-mediated dUTP nick-end labeling) system (Promega).
-
bioRxiv - Synthetic Biology 2019Quote: ... Poly-C tails were added to the fragmented DNA using terminal deoxynucleotidyl transferase (Promega). Then ...
-
bioRxiv - Plant Biology 2023Quote: ... emission wavelength MU: 455 nm) using a fluorometer (Glomax-Multi+ Detection System with InstinctR Software, Promega). The promoter activity was determined by calculating the relative LUC/GUS ratio.
-
bioRxiv - Cancer Biology 2019Quote: ... the GSH-Glo™ Glutathione Assay (Promega, Cat# V6911) was used to measure the intracellular reduced glutathione pool (GSH) ...
-
bioRxiv - Cell Biology 2023Quote: ... 50 µL Total Glutathione Lysis Reagent (Promega Cat #TM344) was added to 3 of the wells per treatment condition and 50 µL Oxidized Glutathione Lysis Reagent (Promega Cat #TM344 ...
-
bioRxiv - Molecular Biology 2021Quote: ... we used Magne-GST Pull-Down Systems (Promega, USA). The bait ...
-
bioRxiv - Plant Biology 2020Quote: ... GST-Halo was used as a protein standard (Promega). Incubate 1 μL of reticulocyte extract before binding with beads and 2 μL of extract after the binding was used for each western on a precast gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel ...
-
bioRxiv - Microbiology 2021Quote: ... and processed using the GSH-Glo Glutathione Assay kit (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: ... Human 26S proteasome purified from HEK293 cells was purchased from Promega. The proteasome chaperoning reaction tests were carried out as follows ...
-
ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... HaloTag-GST was used as a negative control antigen (Promega).
-
bioRxiv - Bioengineering 2022Quote: ... cells were lysed with 60 μL of either Total Glutathione Lysis Reagent or Oxidized Glutathione Lysis Reagent (provided in Promega GSH/GSSG-Glo Assay kit, V6611). After lysis ...
-
bioRxiv - Cancer Biology 2019Quote: Cellular glutathione was quantified using the GSH/GSSG-Glo kit (Promega) according to the instructions provided by the manufacturer ...
-
bioRxiv - Cell Biology 2021Quote: ... and mixed with 10 μl of MagneGST™ Glutathione Particles (Promega) for 1.5 h at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... we used the GSH-Glo™ Glutathione Assay Kit (Promega, V6911) and the protocol was followed as described in the manufacture’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: Glutathione was measured using the GSH-Glo Assay from Promega (V6911) according to the manufacturer protocol ...
-
bioRxiv - Physiology 2022Quote: Terminal deoxylnucleotidyl transferase dUTP nick end labelling (TUNEL) assay (Dead End Fluorometric Tunel Assay, Promega corporation, Madison, USA) was performed 3 days after the UUO ...