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Citations for Promega :
51 - 100 of 180 citations for Glutathione S transferase Class mu 26 kDa Isozyme GST Schistosoma Japonicum since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... TUNEL (terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling) staining was performed using the DeadEnd kit (Promega, PRG7130) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: GSH levels were measured using the GSH-Glo™ Glutathione Assay (Promega) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: GSH was determined using the GSH-Glo™ Glutathione Assay (Promega, #V6911). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... The clarified soluble protein suspension was purified using MagneGST Glutathione Particles (Promega) according to manufacturer recommendations ...
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bioRxiv - Developmental Biology 2023Quote: ... GSH measurements were performed using GSH-Glo™ Glutathione Assay kit (Promega) according to the manufacture’s instruction and SperctraMax L (Molecular Devices ...
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bioRxiv - Developmental Biology 2021Quote: ... The GST tag was removed from hTNPO1 using proTEV Plus Protease (Promega), and the protease enzyme was further removed from hTNPO1 protein by Ni-NTA Magnetic Beads (NEB ...
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bioRxiv - Cell Biology 2022Quote: GST-AIMP1 was in-gel digested with Trypsin GOLD (Promega, Madison, WI). Tryptic peptides were separated by reversed-phase chromatography using Eazynano LC II (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... GST-dimerized dynein constructs were labeled with 5 mM Halo-TMR (Promega) in the column for 10 min at room temperature and the unbound dye was washed away with TEV buffer at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... Cytosine tails (C-tails) were added to 1 µg sheared DNA using terminal deoxynucleotidyl transferase (TdT, Promega, Madison, WI). Transposon containing fragments were amplified in a PCR containing DNA from the TdT reaction as template and primers specific to the ColE1 site on the 5’ end of the transposon ...
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bioRxiv - Molecular Biology 2020Quote: ... Paraffin tissue sections mounted on glass slides were used for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL, Promega) and 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Immunology 2022Quote: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was carried out with commercial kit (Promega, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) TUNEL assays were performed with the Deadend Fluorometric TUNEL system (Promega), according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Terminal deoxynucleotidyl transferase dUTP nick end-labeling (TUNEL) staining was assessed using the DeadEnd Fluorometric TUNEL System (Promega #G3250) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Poly-C-tails were added to 1 µg of each end-repaired sample in duplicate using Terminal Transferase (Promega) with a ratio of 9.5 mM dCTP to 0.5 mM ddCTP to limit chain length and DNA Duplicate reactions were combined and purified with SPRIselect beads ...
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bioRxiv - Cancer Biology 2021Quote: GSH levels were computed using the GSH-Glo™ Glutathione Assay (Promega, USA) according to the protocol provided by the vendor ...
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bioRxiv - Plant Biology 2023Quote: ... 1 % Triton-X100) was added to the reactions with MagneGST Glutathione Particles (Promega) in the presence/absence of 10 mM of the indicated sugars ...
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bioRxiv - Microbiology 2020Quote: ... were made by end-labeling one primer of a complementary pair (primers listed in Table 2) using 20 μM Cy5-dCTP and Terminal deoxynucleotidyl Transferase (TdT; Promega). Complementary primers (one labeled with Cy5 and the other unlabeled ...
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bioRxiv - Microbiology 2020Quote: ... were made by end-labeling one primer of a complementary pair (primers listed in Table S3) using 20 μM Cy5-dCTP and Terminal deoxynucleotidyl Transferase (TdT; Promega). Complementary primers (one labeled with Cy5 and the other unlabeled ...
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bioRxiv - Developmental Biology 2020Quote: ... The in situ detection of fragmented genomic DNA was performed according to the DeadEnd colorimetric TUNEL (Terminal transferase-mediated dUTP nick-end labeling) system (Promega). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... were then added to the 3’ ends of all fragments by incubation of 3 µg of size-selected DNA fragments with the recombinant terminal deoxynucleotidyl transferase (rTdT, 30 U/µL, Promega) at 37°C for 1H ...
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bioRxiv - Molecular Biology 2021Quote: ... pancreatic sections were submitted to terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay using a DeadEnd Fluorometric TUNEL system kit (Promega) according to the manufacturer’s instructions ...
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Glycogen Synthase Kinase 3 regulates the genesis of the rare displaced ganglion cell retinal subtypebioRxiv - Neuroscience 2021Quote: ... Apoptosis was detected by terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end labeling (TUNEL) assays using in situ cell death detection kit (Promega). All images were acquired using a Zeiss LSM710 confocal microscope and Zen software (Zeiss).
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bioRxiv - Biochemistry 2020Quote: ... binding was performed between the soluble fraction (supernatant) and glutathione-conjugated magnetic beads (Promega) pre-equilibrated with lysis buffer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The cell glutathione content was determined using the GGSH/GSSH-Glo assay (Promega; V6611) according to manufacturer instructions and luminescence quantified as described above.
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bioRxiv - Cell Biology 2021Quote: ... and apoptosis was measured using the DeadEnd Fluorometric terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) system (Promega, #G3250). Images from each islet graft were obtained using an EVOS FL Autofluorescence microscope (Life Technologies) ...
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bioRxiv - Plant Biology 2022Quote: ... Nick-end labeling of nuclear DNA fragmentation mediated by Terminal Deoxynucleotidyl Transferase (TdT) was performed following the instructions of DeadEnd™ Fluorometric TUNEL System (Promega). Sections were stained in 1 μg/ml propidium iodide (PI ...
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bioRxiv - Molecular Biology 2023Quote: ... The SP3 protocol (26, 27) used for sample clean-up and trypsin (sequencing grade, Promega) was added in an enzyme to protein ratio 1:50 for overnight digestion at 37°C (in 50 mM HEPES).
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bioRxiv - Biochemistry 2022Quote: ... Total glutathione or GSSG was then measured by using the GSH/GSSH Glo kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Beta cell death was determined in islet graft sections stained for insulin or glucagon using the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) method (Promega, Madison, WI) (20 ...
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Retinal affectation in Huntington’s disease mouse models concurs with a local innate immune responsebioRxiv - Neuroscience 2023Quote: Programmed cell death was determined in retinal cryosections by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) using the DeadEnd Fluorometric TUNEL system following the manufacturer’s instructions (Promega, Madison, WI, USA). Next ...
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bioRxiv - Microbiology 2021Quote: ... or α-mouse (C1r/s) (Promega, W402B) antibodies conjugated to horseradish peroxidase ...
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bioRxiv - Physiology 2021Quote: ... terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining was carried out by using the DeadEnd Fluorometric TUNEL System (Promega, Fitchburg, Wisconsin, USA) according to the manufacturer’s protocol.
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bioRxiv - Genetics 2022Quote: ... Total intracellular glutathione was determined using the luminescent-based GSH/GSSG-Glo Assay Kit (Promega; V6611) according to manufacturer’s instructions in a white 96-well plate prepared with 5 μL of sample supernatant (neat or 1:10 diluted ...
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bioRxiv - Microbiology 2023Quote: ... an RNA probe that is the complement to the AS1-S sequence was synthesized from pSPT19 AS1-S using SP6 RiboMAX Large Scale RNA Production System (Promega) and used as a control probe ...
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bioRxiv - Cancer Biology 2020Quote: ... Generation of infectious lentiviral particles and subsequent cell transduction was performed as previously described.26 FuGENE 6 (Promega) was used to transfect select plasmids ...
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bioRxiv - Biochemistry 2023Quote: ... Tryptic peptides were generated by the S-Trap Method using S-Strap: Rapid Universal MS Sample Prep (Co2-mini,Protifi) and Trypsin Gold (Promega, V5280). Samples were then vacuum dried and resuspended in 50 μL of 1% formic acid (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... 1 μg of GST-tagged effector proteins and 2 μl of FluoroTect™ GreenLys tRNA (Promega, USA) were gently mixed with High-Yield Wheat Germ Master Mix ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble GST and His fusion proteins were purified using the MagneGST Protein Purification System (Promega, USA) or the MagneHis Protein Purification System (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... The recombinant GST-OsNAC5 fusion protein was subsequently purified with the MagneGST™ Protein Purification System (Promega), following the provided protocol.
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bioRxiv - Cancer Biology 2023Quote: Cell lysates from equal numbers of cells were analyzed for glutathione using GSH/GSSG-Glo assays (Promega, V6611) according to the manufacturer’s protocol.
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bioRxiv - Systems Biology 2023Quote: ... + P/S + 8 ng/mL rhFGF-basic (Promega #G5071) + 2 ug/mL doxycycline ...
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bioRxiv - Plant Biology 2022Quote: ... His-tagged and GST-tagged recombinant proteins were purified using Promega MagneHis™ Ni-Particles (Promega Cat#V854A) and MagneGST™ Glutathione Particles (Promega Cat# V861A ...
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bioRxiv - Cell Biology 2019Quote: Total and oxidized (GSSG) glutathione were measured using a commercially available luminescence-based kit (GSH-GSSG GloTM Assay, Promega) on a SpectraMax M3 (Molecular Devices) ...
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bioRxiv - Cancer Biology 2021Quote: Measurement of reduced to oxidized glutathione ratio (GSH/GSSG) was performed with luciferase-based GSH/GSSG-Glo assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and the total cellular GSH of each cell line was assessed using the GSH-Glo™ Glutathione Assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The V4 region of the 16S rRNA gene was amplified using the universal primers 515F (5′-GTG CCA GCM GCC GCG GTA A-3′) and 806R (5′-GGA CTA CNN GGG TAT CTA AT-3′) [26] with Taq&Load MasterMix (Promega). PCR reactions ...
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bioRxiv - Molecular Biology 2023Quote: ... and target sites from the TRIP library were amplified by GoTaq G2 Hot Start Green Master Mix (Promega, 26 cycles). Amplicons were purified via gel extraction using a NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel) ...
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bioRxiv - Plant Biology 2019Quote: ... HA- and GST-tagged proteins were synthesized in vitro using the TNT® Coupled Wheat Germ Extract Systems (Promega). For the pull-down assays ...
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bioRxiv - Immunology 2023Quote: ... 105 cells per target population were sorted and kept on ice until further processing using GSH-GloTM Glutathione Assay (Promega), strictly following manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... was added to 3 of the wells per treatment condition and 50 µL Oxidized Glutathione Lysis Reagent (Promega Cat #TM344) was added to the other 3 wells per treatment condition ...