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Citations for Promega :
4901 - 4950 of 5396 citations for Rat Protein Glutamine Gamma Glutamyltransferase 2 TGM2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The mixture was incubated at 25°C for 30 minutes and the production of ADP was measured using the ADP-Glo kinase assay kit (Promega). Vmax was calculated after fitting the kinase activity data to the Michaelis–Menten equation ...
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bioRxiv - Plant Biology 2023Quote: ... Infiltrated leaves were harvested 48 hrs after infiltration and luciferase activity was measured immediately using the Dual-Glo Luciferase Assay kit (Promega). Leaf disks 4 mm in diameter were cut and inserted into white 96 well plates with 75 µl of 1x PBS and 75 µl of luciferase assay reagent ...
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bioRxiv - Plant Biology 2023Quote: ... Five micrograms of total RNA were ligated to the RNA GeneRacer oligo adapter and subjected to reverse transcription utilizing Improm-II Reverse Transcriptase kit (Promega). The cDNA was used for amplification of cleaved PYL6 fragments ...
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bioRxiv - Genetics 2023Quote: ... After a second purification using a Zymo DNA Clean and Concentrator kit the PCR product was used as a template in a T7 RiboMAX express RNAi System (Promega) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Translation reactions were incubated for 23 min at 30 °C and nanoluciferase activity was monitored using the Nano-Glo Luciferase Assay Kit (Promega) in a Microplate Luminometer (Veritas) ...
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bioRxiv - Cell Biology 2023Quote: ... Other parameters of ER Stress induced cell death were measured through immunoblotting or with the Caspase 3/7 Glo Assay Kit (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... The ratios of luciferase activity for the renilla and firefly luciferases were measured 48 hrs (hours) after transfection using the dual- Luciferase reporter assay kit from Promega and a luminometer ...
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bioRxiv - Microbiology 2023Quote: ... and genomic DNA was isolated from overnight cultures (5 mL) using the Wizard Genomic DNA Purification Kit (Promega GmbH, Walldorf) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Genomic extraction for the WT C6706:str2 strain and each of the resistant mutants was performed using Wizard Genomic DNA Purification Kit (Promega). The concentration of DNA was quantified using Nanodrop spectrophotometer (ND-1000) ...
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bioRxiv - Zoology 2023Quote: Total RNA was extracted using the Maxwell 16 LEV Plant RNA Kit with the Maxwell 16 Research Instrument (Promega, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The bacterial cells were collected from 2mL of the culture and the genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). Samples were then sent to Novogene Co. ...
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bioRxiv - Cancer Biology 2023Quote: ... Phenol-chloroform phase separation and chloroform phase separation steps were repeated to remove the RNase and then DNA was purified from the aqueous phase by using Promega kit protocol (Promega Wizard SV Gel and PCR Clean-Up System ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was isolated with the Maxwell RSC viral total nucleic acid purification kit on a Maxwell RSC 48 instrument (Promega). Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast virus 1-step master mix (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... and used as template to generate dsRNA in vitro using the Promega T7 RibomaxTM Express RNAi System kit (Promega, P1320). The dsRNA was precipitated with sodium acetate (300 mM final ...
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bioRxiv - Biochemistry 2023Quote: ... HiBit and firefly luciferase activity were measured simultaneously using a Nano-Glo HiBiT Dual-Luciferase Reporter System kit (Promega, N1630) with purified LgBiT Protein (Promega) ...
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bioRxiv - Biochemistry 2023Quote: ... PCR reaction mixtures were solved in 1% (w/v) agarose gel and purified using Wizard SV gel & PCR Clean-Up kits (Promega). Then ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with or without 50 μM adenosine 5′-triphosphate (ATP) at 30 °C for 30 minutes followed by kinase assays using ADP-Glo Kinase Assay kit (Promega). Data were analyzed using GraphPad Prism 9 ...
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bioRxiv - Cancer Biology 2022Quote: ... for 72 hours and cell proliferation was assayed using the CellTiter 96 Non-Radioactive Cell Proliferation kit and a GloMax Discovery plate reader (Promega). For proteomic mass spectrometry ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were validated by DNA fingerprinting through the University of Colorado Molecular Biology Service Center utilizing the STR DNA Profiling PowerPlex-16 HS Kit (DC2101, Promega)(Extended Data Figure 11).
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bioRxiv - Genomics 2023Quote: DNA was extracted from the agar/cell mixture from the output and control pools using a Wizard Genomic DNA Purification Kit (Promega). The manufacturer’s protocol was followed with two modifications ...
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bioRxiv - Biochemistry 2023Quote: ... Firefly and Renilla luciferase activities were measured using a dual-luciferase reporter assay kit and a GloMax 20/20 Luminometer (Promega). Transfection efficiency was normalized to that of Renilla luciferase ...
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bioRxiv - Biochemistry 2023Quote: ... U87MG cells were authenticated within a year of their use by the Johns Hopkins Genetics Resources Core Facility by short tandem repeat analysis using the PowerPlex 16 HS kit (Promega). Unless otherwise indicated ...
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bioRxiv - Microbiology 2023Quote: Genomic DNAs were purified from 600 µL of overnight bacterial culture using the Wizard HWM genomic DNA prep kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2023Quote: Cell viability was measured using a commercial kit (Cell Titre 96 AQueous One Solution cell proliferation assay; Promega, Madison, USA) that uses a tetrazolium salt-based colorimetric assay [20] ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 μL of stopped reaction samples were mixed with 50 μL of luciferase substrate solution (Luciferase Assay System kit, Promega) in a white flat bottom 96-well microplate ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was extracted from backup samples for transcriptome analysis using Eastep® Super Total RNA Extraction Kit (Promega, USA). Reverse transcription was performed using GoScriptTM Reverse Transcription System (Promega ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the bands were excised from the gel and TA cloned using the pGEM- T easy-vector II cloning kit (Promega). The standard mini-prep step was avoided by using colony PCR ...
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bioRxiv - Microbiology 2023Quote: ... Gene expression was assessed by qPCR using primers listed in the supplemental material Table S6 and the GO Taq PCR kit (Promega). Gene expression was quantified using SYBR Green (Roche ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Quality was assessed with an Agilent Bioanalyser and the quantity was calculated by an RNA-specific kit from Quantus (Promega).
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bioRxiv - Microbiology 2023Quote: High-molecular weight genomic DNA was isolated based on a modified version of Promega’s Genomic DNA Isolation Kit (Promega, A1120) [63] ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from frozen ground tissues using the innuPREP Plant RNA kit (Analytic Jena) and digested with RQ1 DNase (Promega). First-strand cDNA synthesis was conducted on 1 µg RNA using the SuperScript III Reverse Transcriptase (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted using E.Z.N.A.® Plant RNA Kit (R6827-01, OMEGA) following the manufacturer’s instructions and treated with RNase-Free DNase (M6101, Promega) to remove genomic DNA contamination ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assayed starting at Day 1 for 6 days according to the CellTiter-Glo 3D kit (Promega G9683). For the antiandrogen response assay ...
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bioRxiv - Microbiology 2023Quote: ... Total genomic DNA was extracted from the mid-log culture using the Wizard® Genomic DNA Purification Kit from Promega following the manufacturer’s protocol.
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bioRxiv - Systems Biology 2024Quote: ... The assay was repeated three times and was conducted using the Caspase Glo® 3/7 assay kit (Promega, USA) based on the manufacturer’s recommendations.
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bioRxiv - Molecular Biology 2024Quote: Cell growth endpoint analyses were performed using CellTiter96 Aqueous Non-Radioactive Cell Proliferation MTS Assay kit (Promega, Madison, WI, USA) per manufacturer specifications ...
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bioRxiv - Immunology 2023Quote: In situ nick-end labeling of nuclear DNA fragmentation was performed with a TUNEL apoptosis detection kit (DeadEnd Fluorometric TUNEL system, Promega) according to the supplier’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... Leaf samples of the high dyad producing plants were stored at -80°C before DNA extraction with the Wizard genomic DNA purification kit (Promega). A pooled DNA sample (equal mass ...
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bioRxiv - Microbiology 2023Quote: The glycosyltransferase activity of the CslA-GlxA complex was quantitatively measured by using the UDP-GloTM glycosyltransferase assay kit (Promega). This assay detects the content of free UDP ...
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bioRxiv - Microbiology 2023Quote: The intracellular ATP content of bacteria suspended in mineral water was determined using the luciferase-based BacTiter-Glo Microbial Cell Viability Assay kit (Promega). As per the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid carrying cells were cultured overnight in DM250 supplemented with 50 μg/ml kanamycin and DNA isolated using a Wizard Genomic DNA Purification Kit (Promega). Amplification of target genes was carried out using a SYBR Green based qPCR mix consisting of Q5® High-Fidelity 2× Master Mix (NEB) ...
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bioRxiv - Microbiology 2023Quote: ... core and modified histones and transcription factors at the HIV-1 LTR was assessed by quantitative PCR using primers spanning the full promoter (Table 1) with GoTaq qPCR Master mix kit (Promega) in a CFX Connect Real-Time PCR thermocycler (BioRad) ...
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bioRxiv - Biophysics 2023Quote: ... PB-mClover3-H2B::PGK-BSD and PB-mRuby3-NPM1::PGK-Puro were co-transfected with CAG-PBase into HeLa S3 using a FUGENE HD transfection kit (Promega). Stable clones were obtained by sequential Puromycin and blasticidin selection ...
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bioRxiv - Systems Biology 2023Quote: ... Their identity was confirmed through cell line authentication carried out by BMR Genomics (Italy) by amplification of 23 STR loci (PowerPlex Fusion System kit, Promega). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Bioengineering 2023Quote: ... PCR products were confirmed on a 0.8% agarose gel and purified using Wizard® SV Gel and PCR Clean-Up kit (Promega). DNA fragments were quantified using Nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: Caspase3/7 activity was quantified in cell lysates using the Kit Caspase-Glo® 3/7 Assay Systems (G8091, Promega).
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was isolated from peripheral blood samples using the Maxwell RSC Blood DNA kit (Promega, Cat# AS1400 using the Maxwell RSC benchtop automated DNA/RNA extraction instrument (Promega). Total CER-1236 T cells present in the blood was determined using ddPCR as previously described (17) ...
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bioRxiv - Microbiology 2023Quote: ... and then collected and used to quantify lactate dehydrogenase (LDH) release (in a 50 μl aliquot) using the CytoTox 96 Non-Radioactive Cytotoxicity Assay kit (Cat No. G1780, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated for 24hr at 37°C and cell death was measured through a lactate dehydrogenase (LDH) assay using the CytoTox 96 kit (Promega). 50 μL of media was collected from all experimental wells with two replicates each and placed in a fresh 96-well plate ...
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bioRxiv - Biochemistry 2024Quote: The total RNAs from each cell line (LO2 and Nrf1-/- LO2 cells) were isolated and purified with an RNA extraction kit (Promega) and subsequently all samples were sequenced at the Beijing Genomics Institute (Shenzhen ...