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Citations for Promega :
5001 - 5050 of 5396 citations for Rat Protein Glutamine Gamma Glutamyltransferase 2 TGM2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... at 3500 cells/well and cell proliferation over 3-5 day periods was determined by measuring luminescence with CellTiter-Glo® assay kit (G7570, Promega), according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: miRNAs were isolated from ultracentifuged EVs resuspended in 25 ul of phosphate buffered saline (PBS) using the Maxwell® RSC miRNA Plasma and Serum Kit (AS1680, Promega) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: ... The duplicate amplification products of each sample were pooled and purified with the SV Wizard PCR Purification kit (Promega, Madison, WI) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: The cytotoxicity of compounds on HEK293T and Vero cells was evaluated using the CytoTox-Glo™ Cytotoxicity Assay kit (Promega, #G9290), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... hLamp1-mCherry (this study) were prepared using the Ambion mMessage Machine kit using Sp6 (#AM1340) supplemented with RNAse Inhibitor (Promega #N251B), and diluted to 30–80ng/μl for injection into embryos ...
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bioRxiv - Developmental Biology 2020Quote: ... The α-tub∷GFP (Chung et al., 2014) plasmid was injected at 5-10ng/μl after purification using the PureYield Midiprep kit (Promega, #A2492).
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bioRxiv - Zoology 2021Quote: ... The PCR products were detected through 2% agarose gel electrophoresis and purified with an AxyPrep DNA gel recovery kit (Axygen Biosciences, Union City, CA, USA) and then quantified with a QuantiFluor(tm)-ST blue fluorescence quantitative system (Promega, USA). Purified amplicons were pooled in equimolar amounts ...
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bioRxiv - Cell Biology 2021Quote: ... PCR fragments amplified from genomic DNA extracted from the different founder mice using the Wizard SV genomic DNA extraction kit (Promega, A2360), were cloned into a vector using the TOPO TA cloning kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... was synthesized from 1 μg of total RNA, using oligo-dT primers (USB Corporation, Cleveland, USA) and a reverse transcriptase kit (ImPROm-II, Promega, USA), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: Intracellular glutamate was measured in chondrocytes in 96 well plate format utilizing Glutamate-Glo assay kit (J7021, Promega, Madison, WI USA). Luminescence was measured on microplate reader ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was extracted from lung tissue using the Maxwell automated instrument and the Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega). The concentration of RNA and quality were measured with HS Total RNA 15nt.methods (Agilent ...
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bioRxiv - Bioengineering 2022Quote: ... Media was collected and basal rate of glycerol release by the adipocytes was measured using a Glycerol Glo™ Assay kit (Promega) and luminescence was measured according to the manufacturer’s instructions using a plate reader ...
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bioRxiv - Cancer Biology 2022Quote: ... GB02 and GB03 using the Maxwell® 16 Instrument with the Maxwell® 16 Tissue DNA Purification Kit (Promega, Madison, WI). DNA concentration was determined using the Qubit Fluorometer (Life Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... or 1/6th of the diaphragm) using a Maxwell RSC Instrument and Maxwell RSC simplyRNA Tissue Kit (both Promega, Southampton, UK) and complementary DNA (cDNA ...
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bioRxiv - Synthetic Biology 2022Quote: ... The yeast colony was grown overnight in 10 ml of YPD and the gDNA was extracted using the Wizard Genomic DNA Isolation kit (Promega, Switzerland). The PCR reaction was as follows ...
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bioRxiv - Molecular Biology 2022Quote: DNA extraction was also done for all the samples using the Wizard® Genomic DNA Purification Kit according to the manufacturer’s protocol (Cat#A1120, Promega USA).The eluted DNA was quantified by QuantusTM Fluorometer® (Cat# E6150 Promega Technologies ...
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bioRxiv - Microbiology 2022Quote: ... the cells were lysed and firefly and Renilla luminescence were measured following the manufacturer’s directions (Dual luciferase kit, Promega, Madison, WI) on a luminometer (GloMax® 20/20 Luminometer ...
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bioRxiv - Genomics 2022Quote: ... The liquid cultures were centrifuged at 10,000 rpm and DNA was extracted from the pelleted cells using Wizard DNA purification kit (Promega, Madison, USA) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 5 mL of overnight cultures using the Wizard Genomic DNA Purification Kit (Promega Corp., Madison, WI, USA) and DNA concentration was measured using a Qubit™ Fluorometer and Nanodrop 2000 (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Transduction efficiency was assessed by quantifying luciferase activity in cell lysates using a commercial kit (Luciferase Assay System, Promega, Cat. #E1500) and a plate-reading luminometer (SpectraMax i3x ...
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bioRxiv - Microbiology 2021Quote: ... Remaining ATP was readout after 3 h incubation using Kinase-Glo® Luminescent Kinase kit following manufacturer’s instructions (Promega, WI, USA).
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bioRxiv - Plant Biology 2021Quote: ... and 100 μl sample volumes were assayed in triplicate for ATP content using the BacTiter-Glo bioluminescence kit (Promega, Mannheim, Germany). After 5 min incubation in the dark to allow for the decay of plate autofluorescence ...
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bioRxiv - Plant Biology 2021Quote: ... Aliquots of 50 µl were analyzed in triplicate for NAD+ and NADH with the NAD/NADH-Glo Kit (Promega, Mannheim, Germany) in white 96-well plates using a Synergy HTX microplate reader ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA of all the pOXA-48 bearing strains was isolated using the Wizard genomic DNA purification kit (Promega, WI, USA), and quantified using the QuantiFluor dsDNA system (Promega ...
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bioRxiv - Microbiology 2021Quote: ... for 5 min while orbital shaking and using 20 μL of lysate with 20 μL of Luciferase Kit substrate (Promega, E1501).
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bioRxiv - Cancer Biology 2020Quote: ... Plates containing the different transfected cells were incubated for 24 hours at 37°C before the luciferase activity was measured using the Dual-Glo luciferase detection kit (Promega: E2920).
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bioRxiv - Microbiology 2022Quote: ... Screening for recombinant colonies was made by PCR as previously described [66] using the PCR conditions described above and the GoTag kit (Promega, M3001).
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bioRxiv - Genetics 2022Quote: ... DNA was extracted from viral transport medium or directly from tracheal aspirates by Maxwell® RSC Blood DNA Kit per manufacturer’s instructions (Promega Corporation). RNase A was added to samples to remove potential viral RNA ...
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bioRxiv - Immunology 2022Quote: ... The PCR product was extracted from 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to manufacturer’s instructions and quantified by Quantus™ Fluorometer (Promega, USA). Purified amplicons were pooled in equimolar amounts and paired-end sequenced on an Illumina MiSeq PE300 platform (Illumina ...
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bioRxiv - Immunology 2022Quote: The ADCC or cell killing activity of effector cells incubated with anti-NKG2A antibodies with LALA-PG mutation were measured through release of cytosolic LDH from dead target cells using LDH-Glo cytotoxicity assay kit (Promega, J2381). The purified NK cells from normal PBMCs (Zenbio Inc. ...
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bioRxiv - Microbiology 2022Quote: ... Bacterial counts were obtained by comparing the signals from the tested samples with a standard curve prepared by serial dilutions of a standard sample purified using the WizardSV Gel and PCR Clean-Up System Kit (Promega, USA) and subsequently cloned into the pGEM-T vector (Promega ...
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bioRxiv - Evolutionary Biology 2019Quote: ... probe was generated by an in vitro transcription system using primer pairs with T7 and SP6 promoter regions (T7 and SP6 Ribomax™ large scale RNA kit, Promega) (Mlig-pro46 ...
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bioRxiv - Plant Biology 2019Quote: ... Grown cultures were used for DNA isolation using Wizard Genomic DNA Purification Kit following the manufacturer’s instructional manual (Promega, Madison, WI).
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bioRxiv - Pathology 2019Quote: ... Lactate Dehydrogenase (LDH) release was measured regularly (twice per week) using a CytoTox 96® Non-Radioactive Cytotoxicity Assay kit (Promega).
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bioRxiv - Physiology 2019Quote: In vitro transcription was performed using T3 RNA polymerase and other reagents supplied in the RiboProbe In Vitro Transcription System kit (Promega, Italy) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... Root tissue was ground in liquid nitrogen and total RNA was extracted with the Maxwell® 16 LEV plant RNA kit (Promega) in the Maxwell 16 AS2000 instrument (Promega) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Effects of compounds on NR4A2-AB-Gal4DBD activity was determined via end-point luciferase measurement using a Luciferase Assay System kit (Promega, Inc). The response to 6-MP (3-4-fold maximal induction ...
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bioRxiv - Cell Biology 2019Quote: ... We then assessed cell viability of treated and control cells 48 hours post-transfection using the CellTiter Glo 2.0 luciferase assay kit (Promega, Madison, WI) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Luciferase activity was assessed using the dual-luciferase reporter kit (TransGene, China) and performed via a dual-luciferase reporter assay system (Promega, USA).
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bioRxiv - Molecular Biology 2020Quote: ... 0.6 or 1 μg extracted RNA was converted into cDNA by reverse transcription using a High Capacity cDNA Reverse Transcription kit (Promega A3500) or a ReverTra Ace qPCR RT Master Mix (Toyobo) ...
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bioRxiv - Microbiology 2021Quote: ... cytotoxicity tests were performed in Vero E6 cells via MTS assay using the CellTiter 96® Non-Radioactive Cell Proliferation kit (Promega) as previously described (37) ...
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bioRxiv - Cell Biology 2020Quote: ... and results were normalized to living cell numbers as evaluated with the CellTiter 96® AQueous One Solution Cell Proliferation Assay Kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: Total mRNA was isolated from tissues using a Maxwell® LEV simply RNA Cells/Tissue kit (cat# AS1280, Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from samples using Maxwell® 16 automated system (Maxwell® 16 Cell DNA kits, Promega, Madison, WI, USA). Extracted DNA samples were quantified using NanoDropTM 2000 UV-Vis Spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and real-time PCR was performed on 30 ng of DNA with SYBR Green kit GoTaq® qPCR Master Mix (Promega). DNA from non-transduced cell was isolated and amplified at the same time to demonstrate the absence of contamination ...
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bioRxiv - Synthetic Biology 2020Quote: ... we found we did not obtain clean results unless we first used a genomic miniprep kit to purify DNA (Promega Wizard). We inverted the colors for all agarose gels to assist visualization.
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bioRxiv - Synthetic Biology 2021Quote: ... Luciferase activity was assayed in an Omega luminescence plate-reader (Fluostar) with four different gains following the instructions of the Nano Dual-Luciferase® Reporter kit (Promega). A further correction for luminescence bleed-through was applied using the software developed by Mauri et al ...
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bioRxiv - Immunology 2021Quote: CD8+ T cell cytotoxicity was assessed using a Cyto Tox 96® Non-radioactive Cytotoxicity Assay kit (Promega, Cat no. G1780) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: RNA was isolated from single pupal terminal samples dissected at 24 hours APF or 28 hours APF using the Maxwell® 16 Tissue DNA Purification Kit (Promega). Poly-A RNA-seq libraries were generated using a Clontech library preparation kit (040215) ...
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Quantitative three-dimensional nondestructive imaging of whole anaerobic ammonium-oxidizing bacteriabioRxiv - Cell Biology 2019Quote: ... The resulting PCR products were extracted from 2% agarose gel and further purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences) and were quantified by QuantiFluor™-ST (Promega).