Labshake search
Citations for Promega :
4851 - 4900 of 5396 citations for Rat Protein Glutamine Gamma Glutamyltransferase 2 TGM2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... The DAP-seq gDNA library was prepared using KAPA HiFi HotStart ReadyMix with unique index primers. ZmEREB57 was fused to HaloTag using the kit from pFN19K HaloTag T7 SP6 Flexi Vecto (cat. no. G184A) (Promega). ZmEREB57 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260 ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells were transiently transfected with plasmid DNA pHIV-1 NL4·3Δenv-Luc and Spike-Δ19-D614G by using profection mammalian transfection kit (Promega Inc.). After 48 hours ...
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bioRxiv - Genomics 2022Quote: ... DNA used for microarray was isolated from frozen cell pellets (3×106-7×106 cells) using the Maxwell RSC Cultured Cells DNA Kit on a Maxwell RSC 48 instrument (Promega). DNA was genotyped at the Children’s Hospital of Philadelphia’s Center for Applied Genomics using the Infinium Omni2.5-8 v1.3 BeadChip (Illumina ...
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bioRxiv - Neuroscience 2022Quote: Glucose uptake by cultured primary astrocytes was studied using a bioluminescent method with the Glucose Uptake Assay Kit (Promega, J1341) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Hybridization probes were generated by PCR amplifi cation using gene specifi c primers (Table S1) and radiolabeled with the Prime A Gene labeling kit (Promega) following the recommendations of the manufacturer.
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bioRxiv - Microbiology 2022Quote: Cell viability was measured using a commercially available kit [CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, USA)] ...
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bioRxiv - Cell Biology 2022Quote: Three major proteasome activities (chymotrypsin-like trypsin-like, caspase-like) were measured using the Proteasome-Glo Cell-Based Assay Kit (G1180, Promega) as described by the manufacturer ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were passaged every 3–4 days and regularly checked for mycoplasma infections using a GoTaq G2 Hot Start Taq Polymerase kit from Promega.
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bioRxiv - Microbiology 2022Quote: In vitro generated RNA transcripts above were used templates for in vitro translation assays using a Rabbit Reticulocyte Lysate kit (Promega) at the indicated concentrations ...
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bioRxiv - Zoology 2022Quote: ... The sperm suspension was then divided into two samples: one for determining ATP using a CellTiter-Glo Luminescent Cell Viability Assay kit (Promega) and the other for assessing DNA content (to calibrate the ATP content by the number of sperm cells ...
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bioRxiv - Molecular Biology 2022Quote: For Lytic endpoint measurements cells were seeded as mentioned above and NanoLuc abundance was determined via the Nano-Glo HiBit lytic detection kit (Promega) following manufacturers recommendations ...
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bioRxiv - Plant Biology 2024Quote: ... RNA extraction was performed using an RSC 48 RNA extraction robot (Maxwell) and the Maxwell RSC Plant RNA kit (Promega). Although the RNA yield for some of the 64 samples was low ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was extracted using E.Z.N.A.® Plant RNA Kit (R6827-01, OMEGA) following the manufacturer’s instructions and treated with RNase-Free DNase (M6101, Promega) to remove genomic DNA contamination ...
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bioRxiv - Systems Biology 2024Quote: ... The assay was repeated three times and was conducted using the Caspase Glo® 3/7 assay kit (Promega, USA) based on the manufacturer’s recommendations.
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated from 30 mg of ground plant tissue using the SV Total RNA Isolation System Kit (Promega) and its concentration and integrity were assessed using the RNA 6000 Nano Kit on a Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Microbiology 2024Quote: Lytic cell death was indirectly studied by measuring lactate dehydrogenase (LDH) release in cell culture medium using the CytoTox 96 nonradioactive cytotoxicity assay kit (G1780, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Cell growth endpoint analyses were performed using CellTiter96 Aqueous Non-Radioactive Cell Proliferation MTS Assay kit (Promega, Madison, WI, USA) per manufacturer specifications ...
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bioRxiv - Microbiology 2023Quote: ... Total genomic DNA was extracted from the mid-log culture using the Wizard® Genomic DNA Purification Kit from Promega following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assayed starting at Day 1 for 6 days according to the CellTiter-Glo 3D kit (Promega G9683). For the antiandrogen response assay ...
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bioRxiv - Molecular Biology 2024Quote: ... The -1 PRF efficiency was assayed in cultured HEK293 as described previously (Kelly et al. 2020) using dual-luciferase reporter assay system kit (Promega). 24 hr post transfection ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from overnight cultures on GCB agar plates with appropriate components using Wizard Genomic DNA purification kit (Promega) following manufacturer instructions.
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bioRxiv - Molecular Biology 2024Quote: ... Tail DNA from offspring was genotyped for the presence of the BAC transgene by analyzing 100 ng of tail DNA with the GoTaq PCR reaction kit (Promega) according to the manufacturer’s instruction by adding forward primer 5′-GAGGCTCGGGTGCGGCAGCTC −3′ and reverse primer 5′-GTGCAGTGTGAGCACCTGCTGTC-3′ for PCR with an initial denaturation for 3 min at 95 °C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was isolated and purified using the Wizard Genomic DNA Purification Kit protocol for Gram negative bacteria at one-third volume (Promega), then quantified using SYBR Green I Nucleic Acid Stain (Invitrogen ...
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bioRxiv - Immunology 2023Quote: In situ nick-end labeling of nuclear DNA fragmentation was performed with a TUNEL apoptosis detection kit (DeadEnd Fluorometric TUNEL system, Promega) according to the supplier’s instructions.
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bioRxiv - Microbiology 2024Quote: Aliquots of the final populations were thawed and the gDNA was extracted using the Wizard Genomic DNA Purification Kit (Promega). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... In vitro mRNA synthesis was then carried out at 37 °C for 4 h in T7 RiboMAX Large Scale RNA Production System kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2024Quote: ... Lactate in flow-through medium was quantified using the L-lactate assay kit (Biomedical Research Service & Clinical Application, #A-108) and GloMax microplate reader (Promega) as suggested by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: Approximately 5x104 HEK293 cells were seeded on poly-L-lysine coated glass coverslips and transfected with expression constructs for SORL1-WT or SORL1-R953C using Fugene 6 Transfection Reagent kit (Promega). 24h post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Authentication of this cell line was performed by the Johns Hopkins Genetics Resources Core Facility by short tandem repeat analysis using the PowerPlex 16 HS kit (Promega) within a year of study ...
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bioRxiv - Microbiology 2024Quote: ... The PCR product was extracted from 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to the manufacturer’s instructions and quantified using a Quantus Fluorometer (Promega, USA). Normalization was performed the counts of individual OTUs in a sample by dividing the total counts of all OTUs within that sample followed by a multiplication by resulting in relative abundance expressed.
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bioRxiv - Microbiology 2023Quote: ... The inoculum was added to HEK293T cells and incubated at 37 ° C with 5% CO2 and the luciferase signal was with analyzed using luciferase assay kit (Promega).
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bioRxiv - Cell Biology 2023Quote: ... Supernatant was transferred into a fresh tube and used to measure luciferase activity with Dual-Luciferase Reporter Assay kit (Promega) on a Lumat LB9507 luminometer (Berthold Technologies) ...
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bioRxiv - Genomics 2024Quote: ... Transfected cells were cultured under standard conditions for 72 hours before being washed with PBS and lysed and processed using materials provided with the Dual-Luciferase Reporter Assay System Kit (Promega).
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bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin-labelled or Fluorescein-labelled mRNA probes were either prepared as PCR probes with primers listed in Table S4 using the Kit GoTaq green master mix (Promega) or as described (Orgeur et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... For RT-qPCR four micrograms of the extracted RNA from each sample were reverse transcribed with gene specific primers using Goscript reverse transcription kit (Promega). Quantitative PCR was performed using Power SYBR Green (Life Technologies) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 1 uL of the genomic DNA extracted using a Wizard Genomic DNA Purification Kit according to the manufacturer’s instructions (A1120, Promega USA), 1 uL Q5 high-fidelity DNA polymerase ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We isolated and purified genomic DNA from 1 mL of the overnight cultures using the Wizard DNA purification kit (Promega). Short-read sequencing (2 x 150bp paired-end reads ...
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bioRxiv - Microbiology 2024Quote: ... difficile strains carrying sLucopt reporter constructs was mixed with 20 µL of reconstituted Nanoglo substrate (50 fold diluted Nanoglo substrate in kit buffer, Promega) in a white ...
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bioRxiv - Systems Biology 2023Quote: ... Their identity was confirmed through cell line authentication carried out by BMR Genomics (Italy) by amplification of 23 STR loci (PowerPlex Fusion System kit, Promega). Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Bioengineering 2024Quote: ... the 1X Renilla-luciferase substrate mixed with a Stop & Glo buffer (100 µL/well, Promega Dual Glo kit, cat # E1960) was added ...
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bioRxiv - Bioengineering 2024Quote: ... Cell viability and in vitro luciferase expression was evaluated via ONE-Glo + Tox luciferase reporter and cell viability assay kit (Promega) and a multimode microplate reader.
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bioRxiv - Bioengineering 2024Quote: The viability of Exp-HLOs was analyzed by using the CellTiter-Glo® 3D cell viability assay kit (Promega; G9681) following the manufacturer’s recommended protocol ...
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bioRxiv - Biochemistry 2024Quote: Kinase activity was measured in a generic kinase reaction buffer (20 mM Tris pH 7.4, 50 mM KCl, 10 mM MgCl2) using the ADP-Glo assay kit (Promega, V6930) and on a commercial CK2 substrate peptide (EMD Millipore ...
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bioRxiv - Cell Biology 2024Quote: ... caspase-3/7 activities in cells were measured using a luminometric assay kit Caspase-Glo 3/7 (Promega, Madison, WI). The amount of luminescence was measured on the Victor3™ multilabel reader (PerkinElmer Inc.).
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bioRxiv - Cell Biology 2023Quote: ... Necrosis was quantified by measuring release of lactate dehydrogenase in 30 µl samples of the medium and apoptosis by determining cell-associated Caspase3/7 activity using the respective kits from Promega Corp. ...
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bioRxiv - Biochemistry 2024Quote: The total RNAs from each cell line (LO2 and Nrf1-/- LO2 cells) were isolated and purified with an RNA extraction kit (Promega) and subsequently all samples were sequenced at the Beijing Genomics Institute (Shenzhen ...
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bioRxiv - Immunology 2023Quote: ... Luciferase and Renilla signals were measured 48 h after transfection by a Dual-Luciferase Reporter Assay Kit (Promega, No. E1980). Data were analyzed and normalized by the division of firefly luciferase activity with that of Renilla luciferase to eliminate transfection efficiency difference.
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bioRxiv - Immunology 2023Quote: ... viral RNA (vRNA) was isolated from plasma samples using the Maxwell Viral Total Nucleic Acid Purification kit (Promega, Madison WI). Next ...
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bioRxiv - Microbiology 2023Quote: ... Caspase 3/7 enzymatic activity in raw cell lysates was measured using a Caspase Glo 3/7 assay kit (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... Cells were pelleted (2000 g x 5 min) and subjected to genomic DNA (gDNA) extraction using the Wizard Genomic DNA Purification kit (Promega), according to the manufacturer’s recommendations ...