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Citations for Promega :
4701 - 4750 of 6667 citations for Osteopontin Human OPN ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... were collected from each genotype and total RNA was extracted using a Trizol-adapted ReliaPrep RNA Tissue Miniprep Kit (Promega). Reverse transcription was carried out with PrimeScript RT Master Mix (Takara) ...
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bioRxiv - Plant Biology 2021Quote: ... ground in liquid nitrogen and total RNA from approximately 150 mg of ground leaf material was extracted with a ReliaPrep miRNA Cell and Tissue Miniprep System kit (Promega). A stem loop pulsed RT-PCR protocol [8 ...
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bioRxiv - Neuroscience 2020Quote: ... Cell viability was also determined based on lactate dehydrogenase (LDH) activity in conditioned culture media using a commercial kit (G1780, Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Cell viability was assessed 3 days postinfection by MTS assay using a Cell Titer 96 aqueous cell proliferation kit (Promega) or Viral ToxGlo™ (ToxGlo assay) ...
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bioRxiv - Microbiology 2021Quote: High molecular weight DNA was extracted using the Maxwell® RSC PureFood GMO and Authentication Kit as for qPCR (Promega). The high molecular weight DNA was sent to the Functional Genomic Center Zurich (FGCZ ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 4 μg of the extracted material was then used as template to in vitro synthesized viral genomic RNA transcripts using the Ribomax T7 RNA transcription Kit (Promega) and Ribo m7G Cap Analogue (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was also extracted from inactivated grown cultures using a DNA extraction kit according to the manufacturer’s instructions (Promega, USA).
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bioRxiv - Microbiology 2021Quote: Epithelial cytotoxicity was determined by quantification of lactate dehydrogenase activity in A549 culture supernatants at 24 h post fungal challenge using the Cytox 96 Non-Radioactive Cytotoxicity Assay kit (Promega) according to the manufacturer’s protocol and using recombinant porcine LDH enzyme (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... MT4 and TMZR5 cells were transduced with the genes in Fig 2G as before and 96 hours post-transduction the supernatants were harvested to measure toxicity of the expressed ISGs using the CytoTox-Glow kit (Promega). In a separate experiment ...
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bioRxiv - Molecular Biology 2020Quote: For the analysis of cell vitality of Calu-3 cells treated with camostat mesylate or FOY-251 the CellTiter-Glo Luminescent Cell Viability Assay kit (Promega) was used ...
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bioRxiv - Molecular Biology 2020Quote: ... phaffii cells were cultured for 12-14 h and total RNA was isolated using RNA isolation kit (Cat. # Z3100, Promega) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: gDNA was extracted from stationary phase evolved cocultures following revival from frozen stocks using a Wizard Genomic DNA purification Kit (Promega). DNA fragment libraries were constructed for samples from shaking WT-based cocultures A-F and NifA*-based cocultures M-R at generation ∼146 using NextFlex Bioo Rapid DNA kit ...
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bioRxiv - Microbiology 2019Quote: ... the aptamer pool from the 10th round of SELEX was cloned with the pGEMT-easy Cloning and Ligation Kit (Promega). To investigate the effect of counter-selection on the aptamer pools ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from loopfuls of pure MTBC colonies grown on Lowenstein-Jensen media (37) using the Maxwell 16 DNA Purification Kit (Promega). DNA from Nigerian strains was extracted using the Cetyl trimethylammonium bromide (CTAB ...
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bioRxiv - Cancer Biology 2020Quote: ... Caspase3/7 activity was measured 3 days after the treatment with a commercial available kit (Caspase-Glo 3/7 Assay, Promega).
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bioRxiv - Cell Biology 2021Quote: ... genomic DNA from Proteinase K-digested mouse toes was extracted using the Wizard SV genomic DNA extraction kit (Promega, A2360). PCR reactions to amplify a fragment containing the target sites were carried out using the forward and reverse primers (Forward primer ...
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bioRxiv - Biophysics 2020Quote: ... The cell viability at the same wells was determined by using CellTiter-Glo Luminescent Cell Viability Assay Kit (# G7571, Promega). Neutralization efficiency was calculated as relative fluorescence to the conditioned media collected from mock-transfected cells.
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bioRxiv - Cell Biology 2021Quote: ... The RNA concentrations were measured and 1ug RNAs were used to synthesize cDNA using the GoScript Reverse Transcription System kit(Promega).
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bioRxiv - Cancer Biology 2021Quote: ... cells using ITX2 transfection reagent and luciferase activity (renilla and firefly) was measured 24hours later with the Dual Glow-Stop and Glow luciferase kit (Promega) using a BioTek luminometer (Synergy).
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... PCR products were isolated with a QIAquick Gel Extraction kit (Quiagen) and purified fragments cloned into pGEM-T Easy vector (Promega). Specific details for the generated probes are as follow (gene name (accession number) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were loaded with 5 μM CellROX in media for 30 min and loaded and unloaded control cells analysed for mCherry channel signal using flow cytometry as described above GSH and GSSG levels were measured using GSH/GSSG-Glo assay kit from Promega according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... levels of Firefly and Renilla luminescence in cell lysates were measured by Synergy H1 microplate reader according to Promega’s Dual-Glo kit protocol (Promega #E2920). Fold changes of AP-1 activity were presented by the ratio of normalized Firefly/Renilla to control wells.
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bioRxiv - Microbiology 2022Quote: ... DNA extractions were carried out using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, United States of America) following Lehner et al ...
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bioRxiv - Plant Biology 2022Quote: ... Samples representing different stages of the parasitizing process (i.e. root development) were extracted using the Maxwell® RSC Plant RNA kit (Promega) with the Maxwell® RSC Instrument (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... Luciferase activity (Relative Luciferase Units or RLU) was detected at 72h post-infection by using Bright-Glo Luciferase Assay System Kit (Promega) in a Microplate Luminometer (Wallac-Perkin Elmer).
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bioRxiv - Microbiology 2022Quote: ... Luciferase activity (Relative Luciferase Units or RLU) was detected at 72 h post-infection by using Bright-Glo Luciferase Assay System Kit (Promega) in a Microplate Luminometer (Wallac-Perkin Elmer).
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bioRxiv - Biochemistry 2022Quote: ... SpCas9 gRNAs were transcribed at 37 °C for 16 hours using the T7 RiboMAX Express Large Scale RNA Production Kit (Promega). For gRNAs utilized in in vitro cleavage reactions containing SpRY from human cell lysates ...
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bioRxiv - Microbiology 2022Quote: ... Infected cells were incubated for approximately 18-20 hours and luciferase was measured using the Promega BrightGlo luciferase kit following manufacturers’ instructions (Promega). Entry signal was normalized to the average signal for the “no spike” control ...
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bioRxiv - Biochemistry 2022Quote: The luciferase assay described in this work is based on the luciferase assay system kit protocol from Promega (WI, USA) which was performed to monitor the downstream transcription activation potential of the native and mutated MopR.68 The overnight grown cultures of E.coli DH5α cells harboring the recombinant MopRLUC and MopRG148PLUC plasmids were sub-cultured and grown to the log phase till the OD at 600 nm reached ∼0.3 ...
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bioRxiv - Microbiology 2022Quote: The total RNA of tissues was extracted using the Maxwell RSC simply RNA tissue kit (AS1340, Promega, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... HCT116 cell line was treated for 24 h with 10 µM curcumin and RNA was isolated using the Maxwell® 16 IVD system and the Maxwell Simply RNA Tissue Kit according to the manufacturer’s instructions (Promega). RNA was reverse transcribed using the SuperScript® VILO cDNA Synthesis Kit (Invitrogen ...
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bioRxiv - Immunology 2022Quote: The genomic DNA of the 5 assessed M strains were isolated and purified using the Wizard Genomic DNA Purification Kit (Promega). The growing of strains ...
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bioRxiv - Cancer Biology 2022Quote: Caspase 3/7 activity was measured using a luminescence Caspase Glo 3/7 assay kit (G8090; Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: NAD+/NADH and NADPH/NADP+ measurements were performed by enzymatic cycling reaction assay kits (NADH-Glo and NADPH-Glo, Promega). 4×104 cells were resuspend in 50 μL of PBS and were lyzed by adding 50 μL of NaOH-DTAB buffer containing 1% w/v dodecyltrimethylammonium bromide (DTAB ...
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bioRxiv - Cancer Biology 2022Quote: GSH/GSSG measurement was performed by a glutathione S-transferase (GST) enzyme coupled reaction using (GSH/GSSG Glo kit, Promega). Cells (2×104 ...
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bioRxiv - Cell Biology 2022Quote: hCO viability after 6 days of doxo treatment was assessed by CellTiter-Glo® Luminescent Cell Viability Assay Kit (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse DNA was extracted from the ear using the Wizard® Genomic DNA Purification Kit from Promega (Madison, WI, USA), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... ATP content was determined by a luciferase-based ATP bioluminescence assay kit (BacTiter-Glo™ Microbial cell viability assay, Promega). Luminescence values were determined using a 10 sec RLU signal integration time and measured using a Centro XS3 LB960 Luminometer (Berthold Technologies) ...
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bioRxiv - Immunology 2020Quote: ... Genomic DNA from T0 and T9 (IgA+, IgA- and unsorted) samples was extracted using the Wizard Genomic DNA Purification kit (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and reporter activation quantitated as the ratio of firefly luciferase (FFluc)/Renilla luciferase (Rluc) activity measured using the Dual-Glo kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Cell viability assays in response to drug or DMSO treatment were performed using CellTiter-Glo Luminescent Cell Viability kit (Promega) in cells grown in 96-well plates (Greiner ...
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bioRxiv - Molecular Biology 2021Quote: ... Renilla and Firefly Luciferase signals were read in a MicroLumat Plus LB96V luminometer by using the Dual Luciferase Reporter Assay kit (Promega) following the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The total RNA (1-2 μg) was reverse transcribed with a GoScript™ Reverse Transcriptase cDNA reverse transcription kit according to the manufacturer’s instructions (Promega Corporation ...
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bioRxiv - Microbiology 2020Quote: ... RNA was isolated with the Maxwell RSC Viral Total Nucleic Acid Purification Kit on a Maxwell RSC 48 instrument (Promega). Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher ...
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bioRxiv - Microbiology 2020Quote: ... Cells were harvested after another 24 hours and firefly luciferase and Renilla luciferase expression were determined using the Dual-Luciferase Assay Kit (Promega).
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bioRxiv - Plant Biology 2021Quote: Total plant RNA extraction and DNAse treatment were performed using the SV Total RNA Isolation Kit (Promega, Madison, WI, USA), followed by Reverse Transcription with SMART ® MMLV (Takara Bio USA ...
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bioRxiv - Physiology 2020Quote: ... For the RNA sequencing samples the isopropanol phase of TRIzol extraction was transferred to Reliaprep tissue Miniprep kit (Promega, USA) columns to ensure high quality RNA samples were used ...
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bioRxiv - Microbiology 2021Quote: ... Five ml of BHI broth containing 10 μg/ml tetracycline was inoculated with a single colony and genomic DNA was extracted (Wizard DNA extraction kit, Promega). Genomic DNA was sequenced by paired-end joining Illumina (Biomics Platform of the Institut Pasteur ...
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bioRxiv - Cancer Biology 2021Quote: ... 293T cells were transfected with 8xTEAD synthetic YAP/TAZ-responsive promoter-luciferase reporter and treated with BSA or recombinant TNC (5 µg/mL) for 48 h to measure YAP1 signaling activity using Dual-luciferase Reporter Assay Kit (Promega).
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bioRxiv - Immunology 2021Quote: ... Wells were then washed 5 times with wash buffer and the remaining bound cells were detected with the CellTiterGlo kit (Promega) as per the manufacturer’s instructions.