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Citations for Promega :
4601 - 4650 of 6667 citations for Osteopontin Human OPN ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... Membranes were then incubated with 1:10000 HRP-conjugated secondary antibody (Promega #W402B) in TBST + 5% milk for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibody against HaloTag® (G9211; 1:1000) was purchased from Promega (UK).
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bioRxiv - Neuroscience 2023Quote: ... or a 1:100 dilution of mouse anti-βIII Tubulin (Cat # G7121, Promega) antibody ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mouse IgG (H+L) HRP-conjugated (1:10000; Cat. no. W4021, Promega), and anti-rat IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-rabbit IgG (H+L) HRP-conjugated (1:10000; Cat. no. W4011, Promega), anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... the proteins were digested with 1 µg of trypsin/Lys-C mix (Promega) for 16 h at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM CaCl2) and incubated for 4 hours with 1 µg trypsin (Promega) at 37°C and shaking at 1200 rpm ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed using the GoTaq 1-step RT-qPCR system (Promega). Knockdowns were verified using primers described in Table I ...
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bioRxiv - Developmental Biology 2023Quote: ... and rabbit anti-Caspase-3 polyclonal antibody (1:250, Promega/Fisher, PR-G7481) overnight at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... 1 % Triton-X100) was added to the reactions with MagneGST Glutathione Particles (Promega) in the presence/absence of 10 mM of the indicated sugars ...
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bioRxiv - Biophysics 2023Quote: ... and 10% glycerol) and a 1:50 dilution of Nano-Glo substrate (Promega). All 32 μL were then placed in a 384 well plate and luminescence was measured in a Spark Plate Reader (Tecan) ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 1.5 μg receptor and 1 μg GloSensor-22F (Promega). After 24 hours ...
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bioRxiv - Microbiology 2022Quote: ... Larvae were immobilized using a 1% low-melting-point agarose (Promega; Cat#: V2111) solution and covered with Volvic water containing tricaine ...
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bioRxiv - Microbiology 2023Quote: ... GoTaq® Probe 1-Step RT-qPCR System was purchased from Promega (US). SARS-CoV-2 (2019nCoV ...
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bioRxiv - Cancer Biology 2022Quote: ... DNA (1 μL) was PCR amplified in a mastermix of GoTaq Green (Promega) containing specific primers at a final concentration of 0.5 pmol/μL using the cycling conditions ...
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bioRxiv - Developmental Biology 2023Quote: ... 50 µl of 50mM ammonium bicarbonate (pH 8) containing 1 µg trypsin (Promega) was added to beads overnight at 37ºC with shaking ...
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bioRxiv - Immunology 2023Quote: ... COS-1 cells were transfected with pBRmac239 proviral DNA using FuGENE 6 (Promega). At 48 h post-transfection ...
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bioRxiv - Biophysics 2023Quote: ... Liposomes containing phosphatidylserine and PIP2 (3:1 ratio mol/mol) were purchased (Promega) and prepared to a final concentration of PIP2 at 25 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transiently transfected with 1 or 2 µg HaloTag-Ubiquitin (N2721, Promega) or the control vector pHTN HaloTag CMV-neo (G7721 ...
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bioRxiv - Plant Biology 2023Quote: ... samples were digested with Sequencing Grade Modified trypsin (0.5 µg µL-1; Promega) overnight at 37 °C ...
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bioRxiv - Plant Biology 2023Quote: ... followed by Western blotting using Anti-HaloTag monoclonal Ab (1:2000) (Promega, G9211) and THE GST monoclonal Ab (1:20000 ...
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bioRxiv - Synthetic Biology 2024Quote: ... using 1 μL of unpurified template LET and 0.5 μL of FluoroTectTM (Promega) per 10 μL reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1 μl of premix [100 μM amino acid mixture minus methionine (Promega), 100 μM amino acid mixture minus leucine (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... Digestion was performed at 37 °C overnight with 1 µg of trypsin (Promega). After digestion ...
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bioRxiv - Immunology 2023Quote: ... and 1 mg of RNA was reversed into cDNA by reverse transcriptase ( Promega), which reverse transcribed the Moloney mouse leukemia virus ...
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bioRxiv - Cell Biology 2023Quote: ... 30 μL of CellTiter-Glo reagent solution (Promega, diluted 1:6 in water) was added to each well after 24 h and 72 h of compound treatment ...
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bioRxiv - Genetics 2023Quote: ... Digestion was performed overnight at 37°C with 1 µg of trypsin (Promega). The tryptic digestion was stopped by lowering the pH of the reaction below pH 4 by adding extraction buffer (0.1% TFA 2% acetonitrile) ...
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bioRxiv - Neuroscience 2023Quote: ... and dyes used were anti-Halo-Tag (Promega, cat. G928A, 1:200 dilution), FluoTag-X2-anti-PSD95-Sulfo-Cy5 (28)(NanoTag Biotechnologies GmbH ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples incubated with Trypsin / Lys-C mix (1:70 protease:substrate ratio; V5072, Promega) for 4 hours ...
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bioRxiv - Microbiology 2024Quote: ... were digested with a modified MS grade trypsin (Promega; 1:5 enzyme/protein) overnight at 37 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... We used the following primary antibodies: mouse anti-LacZ (1:500 dilution, Promega) rabbit polyclonal anti- cleaved Caspase 3 (1:200 dilution ...
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bioRxiv - Immunology 2024Quote: ... Reactions were performed with the GoTaq Probe 1-Step RT-qPCR System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and digested overnight with a 1:50 ratio of protein:trypsin (Promega, sequencing grade). Detergents were removed through organic-aqueous phase extraction with ethyl acetate ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1 µL RNase One (10 units/µL RNase ONE Ribonuclease solution, Promega) for 1-4 hours at 37 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1 µL RNase One (10 units/µL RNase ONE Ribonuclease solution, Promega) for 1 or 2 hours at 30 °C ...
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bioRxiv - Microbiology 2024Quote: ... horseradish peroxidase-conjugated anti-mouse IgG or anti-rabbit IgG (1:25000; Promega) was reacted for 3 h ...
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bioRxiv - Molecular Biology 2024Quote: ... Trypsin 1:50 (w/w) (Gold Mass Spectrometry grade, Promega, Madison, WI, USA) was added to each sample for proteolytic digestion and left incubating overnight at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... cell supernatants were harvested for RNA extraction with a Maxwell RSV Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI). Then ...
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bioRxiv - Biophysics 2021Quote: HEK293T cells were collected and pelleted 72 hr post-transfection for genomic DNA extraction using the Wizard Genomic DNA Purification Kit (Promega). The target locus was PCR-amplified using AccuPrime Pfx high-fidelity DNA polymerase (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... The resulting libraries were checked for their quality using the High-sensitivity DNA chip using the Agilent 2100 Bioanalyzer (Waldbroon, Germany) and quantified using the QuantiFluor One dsDNA kit (Promega). Paired-end (2x300bp ...
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bioRxiv - Cell Biology 2019Quote: ... to oxidized NAD (NAD+) ratio was determined in hMPCs via a commercially available luminescence-based kit (NAD/NADH GloTM Assay, Promega) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: Glucose uptake was measured based on the detection of 2-deoxyglucose-6-phosphate uptake by a commercially available luminescence-based kit (Glucose Uptake-GloTM Assay, Promega) on a SpectraMax M3 (Molecular Devices) ...
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bioRxiv - Cell Biology 2020Quote: ... and individual colonies were inoculated in Luria-Bertani (LB) media for DNA extraction using DNA miniprep or midiprep kits (Promega). All mutations were verified by Sanger DNA sequencing (Stony Brook University Genomic Facility) ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were produced from pcDNA3-based plasmids at 30 °C for 90 min using the in vitro transcription & translation (IVTT) kit (Promega), following manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Total cell and mitochondrial free cholesterol and cholesterol ester contents were measured using the Cholesterol/Cholesterol Ester-Glo Assay Kit (J3190, Promega) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: Pooled DNA extractions (minimum 90 adult mosquitoes or 3500 L1 larvae) were performed using the Wizard Genomic DNA Purification kit (Promega). A 349 bp locus containing the predicted on-target cleavage site was amplified with primers containing Illumina Nextera Transposase adapters (underlined) ...
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bioRxiv - Microbiology 2019Quote: ... The viability of N2a cells was evaluated by Cell Titer 96 AQueous One Solution cell proliferation assay kits (Promega, G3582) according to the manufacturer’s instruction.
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bioRxiv - Molecular Biology 2021Quote: After 24 hours a luciferase assay was performed according to the instructions of the Dual-Luciferase Reporter Assay System kit (Promega) or the cells were resuspended in TRI Reagent (Sigma-Aldrich).
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bioRxiv - Developmental Biology 2021Quote: ... media was collected at a single timepoint (12 hours) and the Promega Glutamate/Glutamine-Glo kit (Promega cat. no. J8021) was used ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and from the interior tissue (at least 2 mm away from the outermost edge of the specimen) using the Wizard Genomic DNA Purification Kit (Promega), following the manufacturer’s instructions ...