Labshake search
Citations for Promega :
4601 - 4650 of 4652 citations for Mouse Putative Phospholipase B Like 2 PLBD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... RNA was isolated from frozen harvested materials using the SV Total RNA isolation kit with on-column DNA digestion (Promega, Madison, WI, USA). The purity of all RNA samples was confirmed using a NanoDrop2000 instrument (Thermo Scientific) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... senegalensis DNA was extracted from muscle tissues in thoraxes using Maxwell® 16 LEV Plant DNA Kit (Cat. No. AS1420, Promega, WI, USA). Details on extraction and library preparation protocols are provided in the Supporting Text 1.
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bioRxiv - Cancer Biology 2023Quote: gDNA extraction from formalin-fixed paraffin-embedded (FFPE) tissue sections was carried out using Maxwell 16 FFPE plus LEV DNA purification kit (AS1720; Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... coronary effluent was collected for detection and quantification of lactate dehydrogenase (LDH) using the Promega luminescent LDH-Glo 105TM Cytotoxicity Assay kit following manufacturer protocol (Promega, Madison, WI, USA).
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bioRxiv - Neuroscience 2023Quote: ... was reverse transcribed with a GoScriptTM Reverse Transcriptase cDNA reverse transcription kit according to the manufacturer’s instructions (Promega Corporation, an affiliate of Promega Biotecnologia do Brasil, Ltda). Primers were designed and synthesized by IDT-DNA (San Diego ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we digested tissues using Proteinase K enzyme and used a Maxwell® Rapid Sample Concentrator Instrument with Maxwell® 16 Tissue DNA Purification Kit (Promega Corporation). We used 34 cycles polymerase chain reaction (PCR ...
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bioRxiv - Microbiology 2024Quote: ... for intra-subcellular (i.e., vacuolar) conversion of glutamine (Gln) to glutamate (Glu) assay using Glutamine/Glutamate-Glo Assay kit (Promega, Madison, WI; Cat. # J8021) following kit’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Luciferase activities were conducted 24 hours after transfection in the cells treated with or without the TEAD inhibitor CP1 using the dual-luciferase reporter kit (cat. E1910, Promega, Madison, WI, USA). Assays were conducted in triplicates and quantified using PerkinElmer EnVision plate reader ...
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bioRxiv - Biochemistry 2024Quote: ... and tumors were briefly washed with FluoroBrite DMEM prior to submerging in 200 µL furimazine-FluoroBrite (diluted 1:200 from the Promega Nano-Glo kit). L713F-nLuc signals were then immediately measured using nLuc-specific filter settings (470 ± 40 nm) ...
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bioRxiv - Cell Biology 2024Quote: ... BP1425-500) at 37 °C and the plasmid was isolated from single colonies using the PureYield Plasmid Midiprep kit (Promega, Cat no. A2495), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The amplified product was used as a template to synthesize dsRNA using a RiboMAX Large Scale System-T7 Kit (Promega, Madison, WI, USA). The dsRNA was then isolated and purified ...
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bioRxiv - Cancer Biology 2021Quote: ... Total RNA from cell lines was isolated using Maxwell® 16 Instrument and the Maxwell® 16 LEV simplyRNA Cells Kit (Promega Cat.# AS1270) using the protocol provided ...
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bioRxiv - Bioengineering 2022Quote: ... was performed to measure the specific killing activity of anti-HIV CAR-T cells toward LHL2/3 cells and LEL6 cells at different ratios from 1:1 to 10:1 by using the CytoTox 96 non-radioactive cytotoxicity kit (Promega, Madison, WI, United States) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Silica-dried samples were processed using a combination of bead beating (Retsch MM400, Haan, Germany) and the Maxwell® 16 DNA Purification Kit (Promega, Madison, WI, USA) and are described in detail in supplementary information ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Individual genotyping at every generation was performed via PCR on genomic DNA extracted from larvae pseudopods (Wizard® Genomic DNA Purification Kit, Promega, Madison, WI, USA) using gene-specific primers (forward ...
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bioRxiv - Cancer Biology 2019Quote: ... DNA was extracted from the tumor tissue and blood samples previously frozen using Maxwell® RSC Tissue DNA Kit (cat# AS1610, Promega, Madison, WI, USA) and Maxwell® RSC Blood DNA Kit (cat# AS1400 ...
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bioRxiv - Physiology 2019Quote: Total RNA in the root tips (0-10 mm) was extracted using Eastep® Super Total RNA Extraction Kit (Shanghai Promega Biological Products Ltd). Gene-specific primers were designed by the National Center for Biotechnology Information (NCBI ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... Proteins were expressed from the pcDNA™3.1/His construct by the TNT® T7 Quick Coupled Transcription/Translation kit (Catalog #L1170, Promega Co., Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... was reverse transcribed with a GoScript™ Reverse Transcriptase cDNA reverse transcription kit according to the manufacturer’s instructions (Promega Corporation, an affiliate of Promega Biotecnologia do Brasil, Ltda). Primers were designed and synthesized by IDT-DNA (San Diego ...
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bioRxiv - Microbiology 2022Quote: ... The resulting suspension was then centrifuged at 14,000 rpm at 4°C to remove cellular fragments and the genomic DNA finally was extracted using the Wizard® Genomic DNA Purification Kit reference A1125 (Promega, Madison, WI, USA) following the bulla guidelines ...
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bioRxiv - Microbiology 2022Quote: ... then purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) and quantified using a Quantus™ fluorometer (Promega, Madison, WI, USA).
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bioRxiv - Microbiology 2019Quote: ... genomic DNA were isolated from both the parental UA159 and each of the seven RSV3s using Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, USA) with some modifications ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from overnight bacteria cultures from resistant and wild type parental strains using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, USA), quantified and measured for DNA quality by Qubit High Sensitive dsDNA assay (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... purified amplicons from each single-plex TaqMan® qPCR were cloned into a plasmid vector using the pGEM®-T Easy Vector System I kit (Cat.# A1360, Promega, Walldorf, Germany) and One Shot® TOP10 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... AccuPrep® Plasmid Mini Extraction Kit (Bioneer Corporation, Republic of Korea) and Wizard® SV Gel and PCR Clean-Up System (Promega Corporation, USA) were used for plasmid and PCR product extraction and purification ...
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bioRxiv - Pathology 2020Quote: Messenger RNA was isolated from cells or slices (three slices per sample, pooled, homogenized prior to extraction) using Maxwell® LEV Simply RNA Cells/Tissue kit (Promega, Madison, Wisconsin, US). A NanoDrop® ND-1000 Spectrophotometer (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from each sample taken from captured birds and carcasses using the Wizard Genomic DNA Purification kit (Promega Corp., Madison, Wisconsin, USA) according to the manufacturer’s instructions and eluted in 125 µl of DEPC-treated water ...
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bioRxiv - Plant Biology 2020Quote: Degenerate primers specific to each gene were designed using Primer3plus software [https://primer3plus.com/] for all 17 identified CKX genes following the guidelines for primer design provided by the PCR amplification kit manufacturer (Promega Corporation, USA; Table S2). PCR amplification of the identified CKX GFMs was performed using the reaction kits selected based on the gene size ...
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bioRxiv - Physiology 2020Quote: ... were isolated from cycling Pdgfrb-BAC-eGFP mice by FACS and RNA extracted using the Maxwell® SimplyRNA cells kit (Promega, Cat. No. AS1270). cDNA was amplified using the NuGEN Ovation V2 system and all purified samples were judged to be of high quality and sufficient quantity for next generation mRNA sequencing using the LabChip method (Perkin Elmer) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... One microgram of total RNA from each sample was reverse transcribed into first-strand cDNA by using the M-MLV kit (Promega Corporation, Madison, WI, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Primers flanking the upstream and downstream regions of each gene of interest were amplified from PA14 genomic DNA (Promega Wizard Genomic DNA Purification Kit) and extracted with GeneJet Gel Extraction Kit (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... Colonies were collected and subjected to total DNA extraction using the Maxwell® 16 Tissue DNA Purification Kit in the Maxwell 16 Research Instrument (Promega, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragments such as PCR products or DNA fragment as an agarose gel slices were purified using the Wizard® SV Gel and PCR Clean-Up System kit from Promega (Madison, WI, USA). DNA was digested with the appropriate restriction endonucleases (FastDigest ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: We extracted total RNA by combining a TRIZOL-chloroform approach with a spin column kit (SV Total RNA Isolation System, Promega (Peuß et al., 2016)) ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA isolation was performed by Maxwell® 16 Instrument configured with the LEV High Strength Magnetic Rod and Plunger Bar Adaptor and Maxwell® 16 LEV simplyRNA Purification Kit (PROMEGA, WI, The US) by following the supplier’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA from the bacterial strains used as positive controls was extracted using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, U.S.) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Cell pellets resulting from later experiments (optimization of medium composition and fiber- and drug-testing) were pre-processed using the Maxwell® HT 96 gDNA Blood Isolation Kit (Promega AG, Dübendorf, Switzerland) combined with a FastPrep homogenizer (FastPrep-24TM ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA and RNA were extracted from cell pellets using the Maxwell® RSC Cultured Cells DNA and RNA Kit (Promega, AS1620 and AS1390, respectively) with the Maxwell® RSC 48 Instrument (Promega ...
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bioRxiv - Genomics 2023Quote: ... DNA extraction from pre-enrichments and selective enrichments was done using the Promega Maxwell® RSC cultured cells DNA kit (Promega, WI, USA, AS1620) according to the manufacturer’s specifications on the Promega Maxwell® RSC 48 Instrument ...
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bioRxiv - Evolutionary Biology 2024Quote: ... fruticulosa plants hydroponically cultivated under 0 mM or 150 mM NaCl was extracted using the Maxwell plant RNA kit (Promega Corporation, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: The effect of menadione on the induction of oxidative stress was determined by measuring the ratio between oxidized and reduced glutathione (GSH/GSSG) using a commercial kit by Promega (GSH/GSSG-Glo Assay, V6611).
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bioRxiv - Immunology 2020Quote: ... For the luciferase assay we mixed 20 μl of those supernatants with 100 μl of the luciferase assay reagent (Promega, Luciferase Assay System Kit #E1500) in a well of a white ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from the OP and CL swab samples collected from chickens and tufted ducks using Maxwell® 16 Viral Total Nucleic Acid Purification Kit on a Maxwell® 16 System extraction robot (Promega, Madison, WI, USA). RNA was isolated from lung ...
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bioRxiv - Physiology 2023Quote: ... 200 ng of total RNA from each sample was reverse transcribed into cDNA using random hexamer primers and GoScript™ Reverse Transcriptase kit (Promega Corporation, Madison, WI, USA). The synthesized cDNA was stored at -20 ℃.
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bioRxiv - Microbiology 2023Quote: ... DNA extraction of fecal cultures was performed using the Maxwell® RSC instrument and the Maxwell® RSC PureFood GMO and Authentication Kit (Promega AG, Dübendorf, Switzerland). Library preparation ...
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bioRxiv - Bioengineering 2023Quote: The 36PillarPlate with HLOs was sandwiched with an opaque white 384-well plate containing 20 μL of CellTiter-Glo® Reagent from CellTiter-Glo® cell viability kit (Promega, Madison, WI, USA) and 20 μL of cell culture medium in each well to measure cellular adenosine triphosphate (ATP ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated with compound at 37°C for 72h before multiplexed quantitation of toxicity using CellTox green™ and cell viability using Cell-TiterGlo® (both assay kits from Promega Corp, Madison WI). As a positive control for toxicity 24h prior to cessation of the assay 0.5 mM NaN3 was added to half of the vehicle wells to induce cell death ...
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bioRxiv - Genomics 2019Quote: mRNA was isolated from frozen samples using the Maxwell® 16 AS2000 instrument with a Maxwell® 16 LEV simplyRNA Tissue Kit (both Promega Corporation, Madison, WI, USA). The mRNA was isolated according to protocol with a modified lysis step as described in Snoek et al ...
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bioRxiv - Bioengineering 2022Quote: ... cells were lysed with 60 μL of either Total Glutathione Lysis Reagent or Oxidized Glutathione Lysis Reagent (provided in Promega GSH/GSSG-Glo Assay kit, V6611). After lysis ...
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bioRxiv - Zoology 2023Quote: ... Skeletal architecture was examined by hand cutting tissue sections and digesting them with a mixture of 97% Nuclei Lysis Solution (Promega; from the Wizard DNA isolation kit) and 3% 20 mg/ml Proteinase K (Promega) ...