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Citations for Promega :
4301 - 4350 of 4652 citations for Mouse Putative Phospholipase B Like 2 PLBD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... we found we did not obtain clean results unless we first used a genomic miniprep kit to purify DNA (Promega Wizard). We inverted the colors for all agarose gels to assist visualization.
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bioRxiv - Synthetic Biology 2021Quote: ... Luciferase activity was assayed in an Omega luminescence plate-reader (Fluostar) with four different gains following the instructions of the Nano Dual-Luciferase® Reporter kit (Promega). A further correction for luminescence bleed-through was applied using the software developed by Mauri et al ...
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bioRxiv - Immunology 2021Quote: CD8+ T cell cytotoxicity was assessed using a Cyto Tox 96® Non-radioactive Cytotoxicity Assay kit (Promega, Cat no. G1780) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: RNA was isolated from single pupal terminal samples dissected at 24 hours APF or 28 hours APF using the Maxwell® 16 Tissue DNA Purification Kit (Promega). Poly-A RNA-seq libraries were generated using a Clontech library preparation kit (040215) ...
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Quantitative three-dimensional nondestructive imaging of whole anaerobic ammonium-oxidizing bacteriabioRxiv - Cell Biology 2019Quote: ... The resulting PCR products were extracted from 2% agarose gel and further purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences) and were quantified by QuantiFluor™-ST (Promega).
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bioRxiv - Plant Biology 2019Quote: ... The PCR products were purified using a HiPure Gel Pure DNA Mini Kit (Magen, D2111) and cloned into the pGEM-T Easy Vector (Promega, A1360). For each sample ...
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bioRxiv - Synthetic Biology 2019Quote: Measurements of luminescence intensity from the amber initiator plasmid system was performed as before15 using the NanoYGlo Luciferase Assay System kit (Promega, #N1110) with the following modifications ...
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bioRxiv - Genomics 2021Quote: ... the strain was cultivated on PD2 medium as described (Su et al., 2016) for DNA extraction using Wizard Genomic DNA Purification Kit (A1120; Promega, USA). For Illumina sequencing ...
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bioRxiv - Genetics 2020Quote: Yeast genomic DNA was prepared from 1 ml cultures grown in rich media from single colonies or Ade− half-sectors using the Wizard® Genomic DNA Purification Kit (Promega). Genomic DNA (150 ng ...
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bioRxiv - Genetics 2020Quote: Molecular confirmation of HDR-mediated target site integration of the Reckh cargo was performed on genomic DNA extracted from single GFP+ black-eyed individuals using the Wizard® genomic DNA purification kit (Promega). Primers Kh1-ext-fw (CACTGTTGGCACTCCATCTG ...
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bioRxiv - Cell Biology 2020Quote: ... The resulting lysates were used to measure the humanized renilla (hRluc) and firefly (hluc) luciferase activities with the Dual-Luciferase Reporter Assay kit (Promega, E1960) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... The NAD+/NADH ratio was measured using a protocol adapted from the manual of the NAD/NADH-Glo Assay kit (Promega G9072)2 ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was extracted from 2% agarose gel and purified using the AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, Union City, CA, USA) according to manufacturer’s instructions and quantified using Quantus™ Fluorometer (Promega, USA). The qualified PCR products were mixed ...
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bioRxiv - Cell Biology 2021Quote: ... then left for 48 hrs before measuring the ATP content using CellTiter-Glo® assay kit (Promega, Madison, WI, USA, G7570) [47] ...
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bioRxiv - Physiology 2021Quote: ... Extracts were used for quantification of luciferase as proxy for ATP content using the ENLITEN® ATP Assay System Bioluminescence Detection Kit (Promega) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2020Quote: To isolate RNA from cell lines we followed the manufacturer's instructions of the Maxwell 16 LEV simply RNA Kit (# AS1280) (Promega, Walldorf, Germany). Quantification of mRNA was performed using the BRYT Green® Dye (GoTaq® qPCR ...
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bioRxiv - Genetics 2021Quote: Total RNA was extracted from cultured cells or frozen adipose tissue using the Eastep Super Total RNA Extraction Kit (Promega (Beijing) Biotech Co. ...
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bioRxiv - Cancer Biology 2021Quote: ... The viability of the cultures was determined 24 h and 48 h after EV treatments by using the CellTiter-Fluor cell viability assay kit from Promega (G6080) following manufacturers instructions ...
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bioRxiv - Microbiology 2021Quote: ... supernatant was harvested from Calu-3 cells and inactivated by addition of lysis buffer (Maxwell 16 viral total nucleic acid purification kit, Promega #AS1150) complemented with proteinase K ...
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bioRxiv - Microbiology 2021Quote: RNA from lysed and heat-inactivated samples was purified according to the instructions of the manufacturer using Maxwell 16 viral total nucleic acid purification kit (Promega, #AS1150) or Maxwell 16 LEV simplyRNA cell kit (Promega ...
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bioRxiv - Cancer Biology 2021Quote: Apoptosis of cells cultured in vitro were assessed using a cleaved caspase3/7 activity kit following the manufacturer’s instructions (Promega, cat#8090). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Cell viability was assessed three days post-infection by MTS assay using the CellTiter 96® Aqueous Non-Radioactive Cell Proliferation kit (Promega) and absorption was measured at 495 nm with an EnVision Multilabel Plate Reader (PerkinElmer) ...
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bioRxiv - Genomics 2021Quote: ... small leaves and witches’ broom) (Figure 1B) were collected for total genomic DNA extraction using the Wizard Genomic DNA Purification Kit (A1120; Promega, USA). For Illumina sequencing ...
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bioRxiv - Genetics 2019Quote: ... with a Maxwell 16 instrument using Maxwell 16 LEV SimplyRNA Blood kits according to the manufacturer’s protocol (Promega, Madison, WI, USA). Complementary DNA (cDNA ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was extracted from the aerial portions of 10-day old seedlings using the Wizard® Genomic DNA Purification Kit (Promega) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The radioactively labeled probe was first prepared with γ[32 P]ATP ATP and DNA oligonucleotides listed in Supplementary Table S5 with a T4 polynucleotide kinase kit (Promega), purified on MicroSpin G-25 (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: Total RNA from each lung homogenate and nasal wash was isolated using the Maxwell RSC Maxwell RSC Viral Total Nucleic Acid Purification Kit (Promega Corporation), following the manufacturer’s suggested protocol ...
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bioRxiv - Molecular Biology 2022Quote: Cell death by apoptosis was measured using Caspase-Glo 3/7 luminescent assay system kit according to the manufacturer’s instructions (Promega Madison, WI). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... The first strand cDNA was reverse transcribed from the RNA templates using the GoScript Reverse Transcription system kit (Promega, United States) and Oligo(dT)15 primers (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... by quantifying the activity of lactate dehydrogenase (LDH) released into the supernatant using a colorimetric assay (CytoTox 96® Non-Radioactive Cytotoxicity Assay kit from Promega) and following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from fBECs of WT and Gpr126iECKO mice at P18 using Maxwell RSC simplyRNA tissue kits with the Maxwell RSC Instruments (Promega Corporation). The amount of RNA was measured using the Nanodrop technique and its integrity assessed using Agilent Bioanalyzer 2100 with Nano RNA kits (RIN > 8) ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from fBECs from WT and Gpr126iECKO mice at P18 using Maxwell RSC simplyRNA tissue kits with a Maxwell RSC instrument (Promega Corporation). The amount of RNA was measured using the Nanodrop technique and reverse transcribed with random hexamers (High-Capacity cDNA Archive kits ...
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bioRxiv - Genomics 2022Quote: ... The efficiency and specificity of the designed primers was tested through PCR using the GoTaq Green Master kit (Promega, Madison, USA) with thermal cycling conditions of 2 min at 95 °C ...
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bioRxiv - Genomics 2022Quote: ... DNA was extracted from a piece of caudal fin using a Maxwell® RSC Blood DNA Kit (Promega, Madison, WI, USA). Extractions were performed following the manufacturer’s instructions with the exception of a longer two-hour lysis step ...
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bioRxiv - Evolutionary Biology 2022Quote: ... H12) of each 96 well plate were pooled prior to DNA extraction using Wizard Genomic DNA purification kit (Promega, Madison, Wisconsin). The P0 region including the beginning of galK was amplified for 25 PCR cycles using primers deep_seq_Fw and deep_seq_Rv carrying 5’ adaptors for Illumina sequencing ...
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bioRxiv - Molecular Biology 2022Quote: ... the mycelium was harvested and then used in the RNA extraction following the protocol provided in the Maxwell® 16 LEV simplyRNA Cells Kit (Promega). RNA concentrations were measured by NanoDrop and purified RNA was stored at -80°C ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then pelleted and used for genome extraction using the Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA) and Qiaquick PCR purification kit (Qiagen ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were washed once with 200 µl of dPBS and subsequently analyzed using a Dual-Luciferase Reporter Assay System kit (Promega E1910) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Microbiology 2023Quote: ... One aliquot was used for DNA extraction using the Promega Wizard® Genomic DNA Purification kit (Promega Corporation, Madison, WI, USA) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... of virus pool stock was used for viral RNA extraction by GeneAid viral RNA extraction kit (GeneAid) and converted to cDNA using ImProm-II™ reverse transcription system (Promega) according to manufacturers’ protocols ...
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bioRxiv - Neuroscience 2023Quote: Four to five organoids were physically lysed using a pipette and purified for RNA on the Promega Maxwell RSC48 in conjunction with the Maxwell RSC Simply RNA Tissue kit (Promega, AS1340). cDNA synthesis was performed using the SuperScript III First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Zoology 2022Quote: ... and the other for assessing DNA content (to calibrate the ATP content by the number of sperm cells) using CellTox™ Green Cytotoxicity Assay kit (Promega). After the samples were prepared according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Extracellular lactate was measured according to the manufacturer’s protocol using the Lactate-Glo™ Assay kit (J5021, Promega, Leiden, The Netherlands).
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bioRxiv - Molecular Biology 2022Quote: ... and 3μg RNA was used to synthesize the first strand cDNA in a 20μl reaction using the Reverse Transcription System kit (Promega, Beijing, China). The GoTaq® qPCR and RT-qPCR Systems kit (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor DNA was extracted from parts of FFPE containing a high fraction of tumor cells using Maxwell® RSC DNA FFPE Kit (Catalog number: AS1450, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... supernatant was collected from the replicon-transfected cells and assayed for Renilla luciferase activity according to kit instructions (Promega, cat. E2810).
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bioRxiv - Biochemistry 2024Quote: ... eloB and eloC) were purified on an agarose gel using the Wizard ® SV gel and PCR clean-up system purification kit (Promega). After restriction with the corresponding restriction enzymes (New England Biolabs ...
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bioRxiv - Zoology 2024Quote: ... The amplicon target was amplified from WNV cDNA using the qPCR primers with the forward primer flanked by T7 sequence (5’-TAATACGACTCACTATAGGGATTCGGGAGGAGACGTGGTA-3’) and transcribed using T7 RiboMAX Express Large Scale RNA Production System kit (Promega, France). RNA was purified by ethanol precipitation ...
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bioRxiv - Neuroscience 2024Quote: NAD and NADP levels were measured using the commercially available kits NAD/NADH-Glo Assay and NADP/NADPH-Glo Assay (Promega G9071, Promega G9081), respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were treated with increasing concentrations of CMLD012824 and analyzed using a RealTime-Glo Annexin V Apoptosis and Necrosis Assay kit (Promega JA1011) at 1 to 72 h ...