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Citations for Promega :
4351 - 4400 of 4652 citations for Mouse Putative Phospholipase B Like 2 PLBD2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... T cell cytotoxicity was assessed by quantification of LDH levels in the supernatants using the LDH-Glo cytotoxicity kit (Promega, J2380) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... cells were treated with increasing concentrations of CMLD012824 and analyzed using a RealTime-Glo Annexin V Apoptosis and Necrosis Assay kit (Promega JA1011) at 1 to 72 h ...
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bioRxiv - Microbiology 2023Quote: ... We used 1 ml of cultures and isolated the genomic DNA using the Wizard Genomic DNA Purification Kit (Promega; Madison, WI). We used the DNA with the Illumina DNA Prep kit (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Treatments did not affect bacterial or cell viability as assessed by Lactate dehydrogenase release in the culture supernatant (LDH cytotoxicity kit®, Promega, according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Activities of firefly luciferase (LUC) and Renilla luciferase (REN) were examined using a Dual-Luciferase Reporter Assay System kit (Promega, E1960). LUC/REN was calculated as the relative activity and the ratio of dark / HL was used to indicate light response ...
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bioRxiv - Biochemistry 2022Quote: Cell lysates were prepared as per the manufacturers protocol using the CellTiter-Glo® Luminescent Cell Viability Assay kit (Promega: G7570). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... 3 ml overnight cultures were subjected to DNA extraction using an automated Maxwell® 16 Tissue DNA purification kit and following the manufacturer’s instructions (Promega, UK). For long-read complete genome analysis ...
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bioRxiv - Immunology 2023Quote: ... Cells were collected after 24 hours and luciferase assays were performed using a dual-specific luciferase assay kit (Promega, WI, US) according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... 1 µl of duplex DNA was ligated into 25 ng of pBract214m-HvCas9-HSPT vector linearized with AarI using the ligation reagents and protocol described in pGEM®-T Easy Vector System kit (Promega). The ligation reaction was incubated overnight at 4°C then transformed into competent E ...
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bioRxiv - Immunology 2023Quote: Culture supernatants from the different BMDMs samples were analyzed with the CytoTox 96® Non-Radioactive Cytotoxicity Assay Kit (Promega# G1780). Total cell lysate of non-treated (i.e. ...
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bioRxiv - Cell Biology 2023Quote: Constitutional genomic DNA is extracted from total bone marrow at the remission and purified using magnetic beads (Maxwell® RSC Whole Blood DNA Kit, PROMEGA). Tumor genomic DNA is extracted and purified using magnetic beads (Maxwell® RSC Tissue DNA Kit ...
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bioRxiv - Cell Biology 2023Quote: ... the luminescence of 50 µl of cell lysate was measured using the Nano-Glo Luciferase Assay kit (Promega, Madison, WI, USA) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... Subsequently we ran the digested fragment on a 1% TAE agarose gel and the desired ∼14 kbp DNA fragment was isolated from an agarose gel using a gel purification kit (Promega, A9282).
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bioRxiv - Cancer Biology 2023Quote: ... washed with PBS and inflammasome activation was assessed using qPCR or inflammasome caspase-1 activity was measured using Caspase-Glo® 1 inflammasome assay kit (G9951; Promega) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were cultured for another 5 days before proceeding with microscopy image and viability test by CellTiter-Glo 3D kit (Promega: G9681) following manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... a calibration curve for SAH was constructed in two independent experiments using from 0 to 5 μM SAH according to the manufacturer’s protocol of the MTase-Glo kit (Promega, Wisconsin, USA). The signal was detected using a Tecan Spark microplate reader (Tecan Japan Co. ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were pretreated with indicated antagonist for 1hr before treatment with 10 nM of PGF2a or 100 nM PGE2 overnight before glucose uptake was measured by Glucose Uptake-Glo™ Assay kit (Promega) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... 1 well-1 with 100 μL fresh cell culture media) and mixed with 100 μL reaction solution of CellTiter-Glo 3D cell viability assay kit (G9681; Promega, WI). The 96-well plate was gently rocked for 5 min and stabilized at room temperature for 30 min ...
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bioRxiv - Genomics 2023Quote: ... DNA desulphonation and cleanup steps were immediately performed following the manufacturer instructions of the MethylEdge® Bisulfite Conversion System kit from Promega. After the column cleanup ...
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bioRxiv - Molecular Biology 2023Quote: ... prior to firefly and Renilla luciferase activity measurements which were performed in triplicate using the Dual-GLO kit (Promega cat. #E2920). The whole procedure was repeated three times independently for each construct.
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bioRxiv - Biochemistry 2023Quote: ... After 48 h of incubation at 37 °C and 5 % CO2 culture medium was exchanged with fresh growth medium and Luciferase activity was measured with the Dual-Glo® Luciferase Assay Kit (Promega) according to manufactureŕs instructions using the Tecan Spark® plate reader ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured in a 6-well plate and transfected with 10 µg of linearised plasmid expressing the SV40 Large T antigen using the FuGene Transfection Kit (Promega, #E2691) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... from the access handles by running this on a 1% agarose gel and gel purify the right DNA fragment with a gel purification kit (Promega, A9282).
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bioRxiv - Genomics 2023Quote: ... Resulting genomic DNA was assessed for concentration and purity using Promega DNA quantification kit on the Promega Quantus Fluorometer (Promega, Germany) and measurement of A260/A280 ratio on the MultiSkan Sky High spectrophotometer (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: Notch reporter 4xcsl::H2B-mvenus plasmid(Nowotschin et al., 2013) was injected at 30ng/µl after purification using the PureYield Midiprep kit (Promega, #A2492). For antisense in situ hybridization probes ...
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bioRxiv - Cancer Biology 2023Quote: ... they were treated for the indicated times and viability was assessed using the CellTiter-Glo® 3D Cell viability kit (Promega), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... MDA-MB231 cells were imaged by SEM and quantified by fluorescence analysis of DNA content with the QuantiFluor dsDNA kit (Promega, US). For detection of stem-like properties of cells in scaffolds ...
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bioRxiv - Bioengineering 2023Quote: ... Substrate-dependent differences of cell adhesion and growth were determined by fluorescence analysis of DNA content with the QuantiFluor dsDNA kit (Promega, US) after 5 h and 7 d of culture ...
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bioRxiv - Immunology 2023Quote: Nanoluciferase readings from fecal samples were performed as a proxy for parasite burdens as previously described using the NanoGlo Luciferase reporter assay (NanoGlo Luciferase kit, Promega, N1150) (Vinayak et al. ...
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bioRxiv - Microbiology 2023Quote: DNA extractions for genomic sequencing were performed using Maxwell™ RSC Cell DNA Purification Kit and automated extraction on the Maxwell RSC 48 Instrument (Promega). The DNA concentration was evaluated using a Qubit dsDNA BR assay kit and Qubit Fluorometer (ThermoFisher) ...
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bioRxiv - Genomics 2023Quote: ... leaves and fruits were collected and in the field and total RNA was extracted with Maxwell® RSC Plant RNA Kit (Promega). Two pools were generated and used for PacBio Iso-Seq library preparation (Procedure & Checklist – Iso-Seq™ Express Template Preparation for Sequel® and Sequel II Systems ...
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bioRxiv - Developmental Biology 2023Quote: ... Targeted sequence of each single clone was validated by Sanger sequencing after gDNA isolation through Wizard® Genomic DNA Purification Kit (#A1120, Promega). For Prkaa1 knockout ...
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bioRxiv - Cancer Biology 2023Quote: ... Two replicate electroporations were each divided into three wells for 48h of culture and readout using the Dual-Glo Luciferase kit (Promega; E2940) in a Bio-Tek Synergy HT Microplate Reader.
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bioRxiv - Plant Biology 2023Quote: ... leaf samples of equal size were collected for protein extractions using the Dual-Luciferase Reporter Assay System kit (Promega, Fitchburg, USA). Glomax 96 microplate luminometer (Promega ...
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bioRxiv - Cell Biology 2023Quote: Production of hydrogen peroxide (H2O2) in WT and Alas1 KO brown adipocytes was measured using the ROS-GloTM H2O2 assay kit (Promega #G8820) as per manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... NAD+ and NADH were measured using a protocol adapted from the manual of the NAD+/NADH-Glo Assay Kit (Promega, G9072), as previously described (Li et al. ...
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bioRxiv - Zoology 2024Quote: ... The amplicon target was amplified from WNV cDNA using the qPCR primers with the forward primer flanked by T7 sequence (5’-TAATACGACTCACTATAGGGATTCGGGAGGAGACGTGGTA-3’) and transcribed using T7 RiboMAX Express Large Scale RNA Production System kit (Promega, France). RNA was purified by ethanol precipitation ...
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bioRxiv - Biochemistry 2024Quote: ... eloB and eloC) were purified on an agarose gel using the Wizard ® SV gel and PCR clean-up system purification kit (Promega). After restriction with the corresponding restriction enzymes (New England Biolabs ...
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Sphingosine induction of the Pseudomonas aeruginosa hemolytic phospholipase C/sphingomyelinase, PlcHbioRxiv - Microbiology 2023Quote: We observed induction of the chromosomal plcH-nLuc-plcR reporter in nanoluciferase reporter assays using the Nano-Glo® Luciferase Assay kit (Promega). The assay was conducted as described by the manufacturer with some modifications reported here ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic Acid Purification with Proteinase K digestion was performed using Maxwell ® RSC Total Viral Nucleic Acid Purification Kit according to the manufacturer’s protocols (Promega, USA). For preparation of bacterial genomic DNA ...
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bioRxiv - Genomics 2023Quote: ... cells were washed once with cold 1× PBS and the luciferase activities were measured with Perkin Elmer Envision using Promega Dual Luciferase Assay kit (Promega, E1960). The firefly luciferase activity was normalized to renilla luciferase activity for each well ...
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bioRxiv - Genomics 2023Quote: ... The lysates were generated by adding 100 µL 1X Passive Lysis Buffer included in the Dual-luciferases assay kit (Promega E1960) to the protoplasts and vigorously shaking at room temperature for 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... The amplified PCR fragments were checked for uniformity and size using 0.8% agarose gel electrophoresis and purified using a PCR fragment recovery kit (Promega, WI, USA). The recovered DNA fragments were sequenced using the MiSeq 500PE v.2 kit (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA was prepared from single cell suspensions of thymic leukemias using the Wizard Genomic DNA Purification Kit (Promega, Madison WI). PCR amplification of the Jak3 pseudokinase domain was performed using MyTaq HS Red Master Mix (FroggaBio ...
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bioRxiv - Cancer Biology 2023Quote: ... cytotoxicity and apoptosis were measured 48h and 7 days after the knock-down using ApoTox-Glo triplex assay kit (Promega,G6320) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Amplified ORFs were then used as templates in a PCR reaction to amplify a full-length mutant amplicon followed by their purification using Wizard SV Gel and PCR Clean-Up kit (Promega, USA). These ORFs were digested with KpnI and BamHI enzymes and ligated into similarly digested pDsRed vector ...
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bioRxiv - Bioengineering 2023Quote: The viability of EBs and organoids were measured with a luminescence-based CellTiter-Glo 3D® cell viability assay kit (G9681, Promega) by following the manufacturers’ recommended protocol ...
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bioRxiv - Immunology 2024Quote: ... supernatant was collected from the replicon-transfected cells and assayed for Renilla luciferase activity according to kit instructions (Promega, cat. E2810).
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bioRxiv - Microbiology 2023Quote: ... Cell viability was assessed continuously based on ATP production with the RealTime-GloTM MT Cell Viability Assay kit (Promega, cat# G9711) through bioluminescence output using the TECAN Spark plate reader.
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bioRxiv - Genomics 2023Quote: ... We added 200 µL of PBS to each cell pool and performed the 22 DNA extractions using the Buccal Swab protocol of the ReliaPrep™ gDNA Tissue Miniprep System kit (Promega). We adjusted the lysis time to two hours before performing the RNAse treatment ...