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Citations for Promega :
401 - 450 of 619 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The cells were transferred to white- walled 96-well plates and processed using the Bright-Glo Luciferase Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... lysates were transferred into white 96-well plates and FLuc activity was measured using a commercial substrate (Beetle-Juice, PJK; Luciferase Assay System, Promega) and a plate luminometer (Hidex Sense Plate Reader ...
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bioRxiv - Molecular Biology 2021Quote: ... Luciferase assays were performed on 20 μl of lysate or 1x Cell Culture Lysis Reagent in 96 well plates on the GloMax-Multi Detection System (TM297, Promega). 100 μl Luciferase Assay Reagent was added to wells ...
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bioRxiv - Microbiology 2020Quote: ... 2×105 HeLa-HA cells were seeded per well of a six-well plate and transfected using Fugene-HD transfection reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... Two Days after infection media was removed from the cells and at least 50μl of the CellTiter-Glo® Reagent was then quickly added to all wells in the luminometer plate and the luciferase reading was quantified with a Veritas luminometer (Promega). The cytopathic effect was quantified by the CellTiter-Glo Luminescent Cell Viability Assay system (Promega ...
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bioRxiv - Cancer Biology 2020Quote: HF3016 cells were plated in individual wells of a 96-well plate and viable cells were quantified using CellTiter-Glo 3D Cell Viability Assay (Promega) in triplicate wells as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were seeded in 96 wells plate and luciferase activities were measured using Dual-Luciferase Reporter Assay System kit as described by the manufacture (Promega). Fluc/Rluc ratios were determined as relative luciferase activities.
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bioRxiv - Biophysics 2020Quote: ... mass ratio) into LentiX cells grown to approximately 80% confluency in 6-well plates using FuGENE HD Transfection Reagent (Promega) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... 140 mM NaCl and 1% Triton X-100) and lysates used to assay luciferase activity in 96-well plates using the Dual-Luciferase Reporter Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 25,000 leukemia cells in 250µL of drug-containing medium were seeded in a 48-well plate and viability was assessed every 4 days by using the CellTiter-Glo Luminescent Cell Viability assay (Promega). For RNA-Seq and ChIP-Seq experiments ...
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bioRxiv - Microbiology 2022Quote: ... The plate was incubated in the dark for 2 min and then read on a Glomax Multi+ Detection System (Promega) as above ...
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bioRxiv - Cell Biology 2022Quote: ... The cell plates were incubated at 37°C for 48-60 hours before 50 μL of CellTiter-Glo reagent (Promega) was added ...
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bioRxiv - Microbiology 2022Quote: ... The expression of firefly luciferase was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of neutralization was calculated as the ratio of the reduction in luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Microbiology 2022Quote: ... was mixed 1:1 with cells seeded in 96-well plates for three consecutive days and the luminescence signal was quantified using the GloMax-Multi microplate reader (Promega). A list of all used sgRNA sequences and genotyping primers can be found in Table S4.
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bioRxiv - Biophysics 2022Quote: ... mass ratio) into LentiX cells grown to approximately 80% confluency in 6-well plates using FuGENE HD Transfection Reagent (Promega) per manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... the plates were equilibrated to room temperature and the culture media was replaced by the Nano-Glo HiBiT extracellular reagent (Promega), which was prepared according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were not selected with puromycin but parallel plates were treated with puromycin or left untreated and then cell viability was determined using CellTiterGlo reagent (Promega) for calculation of infection efficiency ...
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bioRxiv - Cancer Biology 2022Quote: ... 25 μL of each were then transferred to a fresh white 96-well plate containing 25 μL of GSH-Glo mixtures (Promega). Luminescence was determined using a Tecan Spark 10 microplate reader
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bioRxiv - Microbiology 2020Quote: ... 20μl of each sample was read on Fluo Star Optima plate reader with injection of 50 μl of Luciferase Assay Reagent (Promega).
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... was performed following the manufacturer’s instructions and absorbance was measured at 490nm using a GloMax-Multi Plate Reader (Promega, E6521). Three independent experiments were performed ...
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bioRxiv - Cell Biology 2020Quote: ... 50 or 100 μl of supernatants was transferred to 96-well plates and ATP was measured using the CellTiter-Glo Luminescent Cell Viability Assay (Promega) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... and the luminescent signal was measured using a CentroXS3 plate reader (Berthhold Technologies) or GloMax explorer multimode microplate reader 3500 (Promega).
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bioRxiv - Biochemistry 2021Quote: ... HEK293T cells and C3H10T1/2 cells were transfected in 6-well plates with various constructs of pmirGlo vector (Promega®). Transfection was performed using Turbofect transfection reagent (Thermo Fischer scientific® ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected in 96-well plates with 98 ng rTetR plasmid and 2 ng pRL Renilla Luciferase control reporter plasmid (Promega) to control for transfection efficiency ...
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bioRxiv - Immunology 2022Quote: ... Cells were transferred to a white-walled 96-well assay plate and mixed with an equal volume of Cell Titer Glo reagent (Promega). Measurements for fluorescence at 544-15 nm (excitation) ...
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bioRxiv - Systems Biology 2022Quote: ... and 72h in 3-5 wells for each condition (mean values of 3-5 technical replicates are provided for each donor) using a multiwell plate reader Glomax (Promega). Each 2h incubation was performed at a multiplicity of infection (MOI ...
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bioRxiv - Immunology 2022Quote: A total of 200,000 RAW cells were seeded on a 24-well plate and were co-transfected with NFATc1(pGL3-NFAT) and Renilla luciferase plasmid (pRL-TK, Promega) in a ratio of (2:1) ...
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bioRxiv - Microbiology 2022Quote: ... was added to the plate and incubated at room temperature for 5 min before measuring luminescence on a GloMax 96 luminometer (Promega).
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bioRxiv - Microbiology 2022Quote: ... and transferring the contents to a 96-well plate to which the appropriate substrate was added and assayed using a GloMax multi system (Promega).
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bioRxiv - Molecular Biology 2020Quote: ... RL and FL activities were measured on 96-well plates using 2 µl of cell extract containing 8 µl of 1x PLB (Promega) and the dual-luciferase assay kit (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were diluted and aliquoted to 96-well plates to make less than 1 cell count per well and lysed by 30 μl of Passive Lysis Buffer (Promega) when confluent ...
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bioRxiv - Molecular Biology 2020Quote: ... Luciferase activity present in 10 µl of lysate clarified by centrifugation was then quantitated in white 96-well luminometer plate (Microlumat Plus 1b 96V instrument, Berthold Technologies) using 50 µl of luciferase assay reagent (Promega).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 40 μl of the cell suspension were transferred to each well of 96-well plates containing 10 μl of 5X GloSensor cAMP Reagent (Promega) prepared according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Sister plates were exposed to drug but not infected to gauge cytotoxicity via CellTiter-Glo assay (CTG, Promega, Madison, WI), 48 hrs post treatment.
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bioRxiv - Molecular Biology 2020Quote: ... Plates were incubated at room temperature for 1 hour before luminescence was measured using a GloMAX Discover Plate reader luminometer (Promega). Three replicate experiments were performed with three biological replicates for each cell type and treatment within each experiment.
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bioRxiv - Molecular Biology 2020Quote: ... was added to each well and the plate was incubated for 10 minutes at room temperature before luminescence was measured using a GloMAX Discover Plate reader luminometer (Promega). Three replicate experiments were performed with three biological replicates for each cell type and treatment within each experiment for nine total measurements per cell type and treatment.
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bioRxiv - Genetics 2020Quote: ... Cell lysates were assayed in triplicate in a 96-well plate and luciferase activity was quantified using a GloMax microplate luminometer (Promega). Percent frameshift was calculated by averaging the three firefly or Renilla luciferase technical replicate reads per sample then forming a ratio of firefly to Renilla luminescence per sample ...
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bioRxiv - Immunology 2021Quote: ... 50 μL of the supernatant was removed from treated wells and the luminescence values were again recorded on a plate reader (Mithras LB 940, Berthold Technologies) in the presence of the nano luciferase substrate furimazine (2 μL/mL, Promega).
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bioRxiv - Microbiology 2020Quote: ... The expression of firefly luciferase was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of infectivity was calculated as the ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Biophysics 2020Quote: ... Plasmids were transfected in cells (passage number < 35) seeded in 96-well plates at ~50 % confluence 2-4 days before the experiment with FuGene6 (Promega) or Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were seeded in triplo in opaque 96-well plates and cell viability was measured 96h later using the GloMax Discover Microplate reader (Promega). Luciferase reporter assay (Promega ...
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bioRxiv - Immunology 2020Quote: ... the cells were washed with PBS and then lysates were prepared by scraping the cells from plates in the presence of 1× Passive lysis buffer (Promega). Luciferase assays were performed according to Dual-Luciferase Assay System (Promega ...
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bioRxiv - Immunology 2020Quote: ... Single CD3-CD8-CD16- CD20+Ova-RBD-PE+RBD-AF647+ B cells were sorted into individual wells of a 96-well plates containing 4 μl of lysis buffer (0.5 X PBS, 10mM DTT, 3000 units/mL RNasin Ribonuclease Inhibitors (Promega, N2615) per well using a FACS Aria III (Becton Dickinson) ...
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bioRxiv - Immunology 2020Quote: ... Plasmid transfections of IFITM1 3’ UTR reporter plasmids (500 ng per single well of a 6-well plate) were performed using the FuGENE 6 (Promega), according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Plates were incubated at 25 °C for 1 hr and fluorescence was measured using a BioTek Synergy Neo plate reader (Promega). The terbium (Tb ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed with PBS and the cells were lysed with 1× reporter lysis buffer (100 µl per well; Promega). Following a freeze-thaw cycle ...
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bioRxiv - Genomics 2021Quote: Cells were transfected in six-well plates (TPP, Switzerland) with 2μg of pSPCas9-2A-CD19 using FuGENE® HD Transfection Reagent (Promega) or Lipofectamine Stem Transfection Reagent (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated for 15 minutes and allowed to shake briefly before ATP was quantified via luminescence readout on the GloMax Explorer multimode plate reader (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... in 6 well plates or 96 well plates (details in the figure legends) and assayed using crystal violet staining or Celltiter-Glo luminescent viability assay (Promega) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... The plates were incubated at 37°C for ~12 hours before luciferase activity was quantified using the Luciferase Assay System (Promega) using SoftMax Pro v.7.0.2 (Molecular Devices) ...