Labshake search
Citations for Promega :
501 - 550 of 619 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... cell survival in each well was quantified on a Tecan Spark plate reader using CellTiter-Glo 2.0 Cell Viability Assay (Promega, G9242). Percent survival was calculated as the ratio of luminescence in the presence versus absence of puromycin for each lentiviral dilution ...
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bioRxiv - Biochemistry 2024Quote: ... 100 µl of medium from each were then transferred to a new 96-well plate and 100 μl of CytoTox 96 colorimetric reagent (Promega) were added to each well and incubated for 30 minutes at room temperature in the dark ...
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bioRxiv - Cancer Biology 2021Quote: ... were seeded in 96-wells white plate and caspase-1 activities were assessed using Caspase-Glo 1 Inflammasome Assay (Promega #G9951) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Cell growth and viability were measured on the 96 well white bottom plate as an end point measurement for each time point and/or treatment using the Cell-titer GLO assay (Promega, # G7570) based on manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed with PBS and lysed directly on the plate on ice using the Cell Culture Lysis Reagent from the Luciferase Assay System (Promega#E1500). Lysates were transferred to microcentrifuge tubes ...
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bioRxiv - Neuroscience 2021Quote: ... neonatal and adult astrocytes plated on 96-well plates (50,000 cells per well) was measured with the Glucose Uptake-Glo™ Assay (Promega, J1341) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... The indicated compounds were introduced analytically using a CyBio pintool and the plates left to incubate at 37 °C prior to the addition of the CellTiter-Glo reagent (Promega, G7573) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2020Quote: 50 μl supernatant were collected as designed time point into a 96-well plate for lactate dehydrogenase (LDH) release assay as manufacture’s protocol (Promega, cat# G1780). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Crystal violet was the solubilized with a 75% ethanol solution and the absorbance was measured at a λ600nm with a plate reader (Promega GloMax Explorer, Promega, France).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were assayed in white 96 well plates in triplicate by the addition of 50 μl Bright-Glo™ Firefly luciferase assay system (Promega) or freshly prepared 40 ng/ml Coelenterazine in Renilla-Glo buffer (Promega) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 25 µL of the lysates were transferred to a white plate and mixed with the same volume of Nano-Glo® Luciferase reagent (Promega). For the A-MLV titers ...
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bioRxiv - Biochemistry 2021Quote: ... WT or ADAR1-KO HEK293T cells in 12-well plates were transfected with 400 ng ml-1 of firefly luciferase under control of the IFN-β promoter (pIFN-Luc, Promega), 40 ng ml-1 of Renilla luciferase under a constitutive promoter (pRL-TK ...
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bioRxiv - Bioengineering 2021Quote: 100 μL of media was removed from each well on the plate and replaced with an equal amount of CellTiter-Glo reagent (Promega, G7572). Assayed plates were shaken at 500 RPM for 20 minutes and allowed to rest for 10 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Media was refreshed on day 6 and plates collected on day 12 with cell viability quantified by CellTiter-Glo 3D (Promega #G9681). Dose response assay analysis was performed as described above.
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bioRxiv - Biochemistry 2022Quote: Intracellular glutamate was measured in chondrocytes in 96 well plate format utilizing Glutamate-Glo assay kit (J7021, Promega, Madison, WI USA). Luminescence was measured on microplate reader ...
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bioRxiv - Genomics 2022Quote: ... F-luciferase and R-luciferase expression was assayed in a TECAN Spark plate reader using the Dual-Glo® Luciferase Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 48 h later assays were then lysed and read on a FLUOstar Omega plate reader (BMF Labtech) using the Steady-Glo® luciferase assay system (Promega)
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bioRxiv - Molecular Biology 2021Quote: ... and cells with approximately 70-90% confluency in each plate were transfected with required plasmid(s) for each assay using Fugene 6 transfection reagent (Promega, USA) or 1 mg/ml PEI (polyethyleneimine).
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bioRxiv - Microbiology 2021Quote: ... Bright-Glo reagent was added to the plate and incubated at room temperature for 5 min before measuring luminescence on a GloMax 96 luminometer (Promega, USA), with titers given in Relative Light Units (RLU ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding each vector were transfected into HEK293 cells seeded in 10 cm plates using Fugene 6™ (Promega, Madison, WI) or polyethyleneimine ...
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bioRxiv - Bioengineering 2020Quote: ... 100 μl of each supernatant were transferred into new 96 well plates and equal volume of BacTiter-Glo™ Reagent (Promega) was added per well ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 25 µL of the lysates were transferred to a white plate and mixed with the same volume of Nano-Glo® Luciferase reagent (Promega). For the VSV-G titers ...
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bioRxiv - Molecular Biology 2021Quote: Cells were plated on a white 96-well plate (Grenier, 655083) and incubated with RealTime-Glo MT Cell Viability Assay reagent (Promega, G9712). The luminescence was detected by GloMax Navigator.
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bioRxiv - Microbiology 2021Quote: ... 30 μl of cell lysis was transferred to a white 96-well assay plate and firefly and renilla activities were measured using the Dual-Glo® Luciferase Assay System (Promega), reading luminescence on a GloMax ®-Multi Detection System (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μL of supernatant was transferred to a new plate and assayed for luciferase activity using the Nano-Glo Dual-Luciferase Reporter Assay System (Promega, N1610) to measure Firefly (Fluc ...
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bioRxiv - Synthetic Biology 2021Quote: ... Luciferase activity was assayed in an Omega luminescence plate-reader (Fluostar) with four different gains following the instructions of the Nano Dual-Luciferase® Reporter kit (Promega). A further correction for luminescence bleed-through was applied using the software developed by Mauri et al ...
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bioRxiv - Immunology 2020Quote: ... Single CD19+IgG+RBD+ cells were sorted into a 96 well plate containing 4 µL lysis buffer (12 units of RNasin Ribonuclease Inhibitor (Promega, N2511), 10 mM DTT and PBS x0.5 ...
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bioRxiv - Immunology 2020Quote: EPC cells were seeded in 24-well plates overnight and cotransfected with various plasmids using FuGENE HD Transfection Reagent (Promega, #E2311) following the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: The remodeling of neuron isolated mitochondria was evaluated according to a published protocol,[54] by measuring the changes in the absorbance of the mitochondrial suspensions at 540 nm using a GloMax Discover multimode plate reader (Promega, Italy). Briefly ...
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bioRxiv - Evolutionary Biology 2022Quote: ... H12) of each 96 well plate were pooled prior to DNA extraction using Wizard Genomic DNA purification kit (Promega, Madison, Wisconsin). The P0 region including the beginning of galK was amplified for 25 PCR cycles using primers deep_seq_Fw and deep_seq_Rv carrying 5’ adaptors for Illumina sequencing ...
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bioRxiv - Microbiology 2022Quote: Activity of caspases-3/7 and -8 were assessed using the corresponding Caspase-Glo® Assays in white-walled 96-well plates (Promega).
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bioRxiv - Synthetic Biology 2022Quote: To measure luciferase activity on plaque containing LB-agar plates, 5 μl of the diluted (1:50, in PBS) NanoLuc® luciferase substrate 2-furyl methyl-deoxy-coelenterazine (Furimazine; Promega) were dropped onto the respective plaques ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µL of the schizont culture was aliquoted into white 96 well luminometer plates to which 45 µL of 1x NanoGlo Lysis buffer with 1:1000 NanoGlo substrate (Promega, USA) was added ...
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bioRxiv - Microbiology 2023Quote: ... and then grown for 24h or 18h with OD600nm measurements every 30 min taken by GloMax Plate Reader (Promega, WI, USA). Anaerobiosis was maintained with a O2-less sealing film (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... drugs were added to plates in triplicate and incubated for 3 days before being developed by adding CellTiterGlo (Promega, Madison, WI) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: We developed a high-throughput cytotoxicity plate based-assay using the Cell Titer-Glo® 2.0 Luminescent Cell Viability Assay (Promega, G9242) to screen CBCS Oncoset collection of 172 compounds with known targets (listed in table S1 ...
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bioRxiv - Cell Biology 2023Quote: ... fluorescence intensity was quantified using a fluorescence plate reader at an excitation of 485 nm and an emission of 538 nm (GloMax® Discover Microplate Reader, Promega).
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bioRxiv - Neuroscience 2023Quote: ... 10µl of luciferase containing culture medium was transferred into a fresh 96 well plate and incubated with either Furimazine (Nano-Glo™ Luciferase Assay, Promega)/Coelenterazine (Prolume ...
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bioRxiv - Immunology 2023Quote: ... Plates were incubated for 22 hours at 37 °C/5 % CO2 prior to addition of 33ul Bio-Glo reagent (Promega G7940), then luminescence was read on a Clariostar plate reader.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... opaque 96-well plates and reagents were added according to the manufacturer’s protocols for the Nano-Glo® HiBiT Extracellular Detection System (Promega Corp.). After a 10-minute incubation step ...
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bioRxiv - Genomics 2023Quote: ... The concentration of 12 wells (diagonal) of the plate containing the diluted cDNA was checked using the QuantiFluor dsDNA System (Promega, E2670) fluorometric assay as the initial cDNA concentration is a critical step to obtain libraries with adequate fragment length ...
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bioRxiv - Immunology 2023Quote: ... Luciferase activity was measured in triplicate per condition in a 96-well plate 24-36 hours post-transfection using the Nano-Glo Dual-Luciferase Reporter System (Promega #N1610) according to the manufacturer’s instructions in a BioTek Cytation5 plate reader ...
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bioRxiv - Synthetic Biology 2023Quote: ... 25 μL of samples was added into white-walled 96-well plates and equal volume of ready-to-use Tluc substrate (Promega; E1501) was injected to each well ...
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bioRxiv - Biochemistry 2023Quote: ... The plates were incubated at 37 °C for 1 hour before 10X stocks of experimental compounds were added and the plates were analyzed on a GloMAX® Discover microplate reader (Promega) in kinetic mode for NanoBRET ratio metric (460 nm donor and 618 nm acceptor emissions ...
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bioRxiv - Genomics 2023Quote: ... and 20 µL of the supernatant was used for the dual-luciferases assay measured in GloMax Navigator Plate Reader (Promega, GM2010) as specified by the manufacturer ...
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bioRxiv - Synthetic Biology 2024Quote: ... Luciferase activity was assayed in an Omega luminescence plate-reader (Fluostar) with four different gains following the instructions of the Nano Dual-Luciferase® Reporter kit (Promega). A further correction for luminescence bleed-through and background signal was applied using the software developed by Mauriet al.58 Then NLuc signal was divided by the FLuc signal to normalize for the transformation efficiency.
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... Infectivity was determined after 3 days by luminescence using a Promega GloMax plate reader following addition of BrightGlo reagent (Promega #E2620) to Calu-3 cells or FLuc Assay buffer (Nanolight #318 ...
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bioRxiv - Microbiology 2024Quote: DNA extraction of the bacterial strains isolated from oysters and cultivated on agar plates was carried with the Wizard® Genomic DNA Purification Kit (Promega) according to the manufacturer instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Measurements were taken every 10 min in Nunc™ F96 MicroWell™ 446 plates using a GloMax® navigator luminometer (Promega) with 5s integration time and 2s delay ...
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bioRxiv - Microbiology 2024Quote: ... 40 µl of supernatants were transferred in a luminometer 96 wells plate and processed with Nano-Glo® Luciferase Assay Reagent according to the manufacturer’s instructions (Promega N1110). The plate was read 3 min after the addition of the reagents on a Berthold luminometer ...