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Citations for Promega :
401 - 450 of 699 citations for Zika Virus Lysate DakArD 41662 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... was in vitro transcribed and translated with a reticulocyte lysate system TnT T7 (Promega, Fitchburg, WI) by incubation of plasmid DNA (1 mg ...
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bioRxiv - Cell Biology 2022Quote: ... Pellets were lysed and bait proteins in the lysates were bound to HaloLink Magnetic Beads (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Gaussia luciferase activity was then measured from 10ul of lysate using the Renilla luciferase kit (Promega) with a FLUOstar Omega plate reader (BMG Labtech) ...
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bioRxiv - Microbiology 2021Quote: ... and Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega) and a Glomax Navigator luminometer (Promega) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and luciferase activity was measured in cell lysates (Renilla luciferase assay system, Promega Madison WI, USA).
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bioRxiv - Microbiology 2020Quote: ... The lysate was then diluted to <2 M urea and incubated with 50 μg trypsin (Promega) overnight at 37 °C under agitation ...
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bioRxiv - Microbiology 2021Quote: ... Firefly luciferase assays were performed on 20 μL of cell lysates using Luciferase Assay System (Promega) according to the manufactures instructions ...
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bioRxiv - Cell Biology 2021Quote: ... free and total cholesterol concentration in lysates was determined using Cholesterol/Cholesterol Ester-Glo Assay (Promega) following manufacturer recommendations ...
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Metabolic switching and cell wall remodelling of Mycobacterium tuberculosis during bone tuberculosisbioRxiv - Microbiology 2022Quote: ... the cell lysate was pelleted and read for RLU using a luminometer (Promega GloMax 20/20).
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bioRxiv - Molecular Biology 2022Quote: ... 50 μL of the lysate were added to 50 μL luc substrate (Promega Luciferase Assay System). The amount of luciferase was quantified by luminescence (relative luminescence units (RLU)) ...
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bioRxiv - Molecular Biology 2020Quote: Constructs for expression in rabbit reticulocyte lysate (RRL) were based on the SP64 vector (Promega, USA). Constructs for translation in the PURE system were generated from the T7 PURExpress plasmid (New England Biolabs ...
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bioRxiv - Neuroscience 2021Quote: ... the luciferase activity in the cell lysates was determined using the Dual Luciferase Reporter System (Promega). Firefly luciferase activities were normalized to that of Renilla luciferase ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were assessed using the Dual-Luciferase Reporter Assay System (#E1910; Promega, Madison, WI, USA). Luminescence was measured using a TECAN M200 infinite-PRO plate reader (Tecan ...
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bioRxiv - Microbiology 2021Quote: ... Luminescence activity in the resulting cell lysates was measured using the Luciferase Assay System (Promega, USA).
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bioRxiv - Molecular Biology 2019Quote: ... and lysates were assayed for dual luciferase activity using the Dual Luciferase Reporter Assay System (Promega) and a luminometer ...
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bioRxiv - Immunology 2021Quote: ... The luciferase activity in cell lysates was measured using the Nano-Glo Luciferase Assay System (Promega) and GloMax system (Turner BioSystems) ...
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bioRxiv - Genomics 2020Quote: DNA extraction was performed on the phage lysate using the Wizard DNA Clean-Up kit (Promega) with minor modifications ...
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bioRxiv - Neuroscience 2021Quote: ... 25 uL of lysate was mixed with NanoGlo substrate diluted 1:50 in NanoGlo buffer (Promega) and 25 uL of lysate was mixed with ONE-Glo luciferase assay buffer (Promega ...
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bioRxiv - Immunology 2020Quote: ... and NanoLuc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega). Relative luminescence units (RLUs ...
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bioRxiv - Developmental Biology 2020Quote: Unlabelled in vitro translated protein was produced using rabbit reticulocyte lysates according to manufacturer’s protocol (Promega).
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bioRxiv - Immunology 2021Quote: ... Luciferase activity in total cell lysates was measured using a dual-luciferase reporter assay system (Promega).
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bioRxiv - Cell Biology 2022Quote: Constructs for expression in rabbit reticulocyte lysate (RRL) used the SP64 vector as a backbone (Promega). For in vitro insertion reactions ...
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bioRxiv - Genetics 2022Quote: ... The lysate was digested overnight at 37 °C with 2 μg of sequencing-grade trypsin (Promega), after which the sample was acidified to 0.1% formic acid ...
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bioRxiv - Systems Biology 2022Quote: ... In-solution digestions were performed on lysates containing 5 mg of protein using either Trypsin (Promega), Chymotrypsin (Promega) ...
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bioRxiv - Biochemistry 2022Quote: ... The mRNA obtained was used for in vitro translation in Flexi Rabbit Reticulocyte Lysate System (Promega) and the resulting lysate was directly used in the import reaction ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell lysates were used to measure Luciferase activities with the Dual-Luciferase Reporter Assay System (Promega) as indicated by the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Nanoluc Luciferase activity of lysates was then measured using the Nano-Glo Luciferase Assay System (Promega) with GloMax Discover System reader (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... Then 50 µL of lysates were mixed to 50 µl D-luciferine (Luciferase assay system, Promega) and the Firefly luciferase activity was determined for each cell lysate with an Infinite 200 Pro (Tecan ...
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bioRxiv - Microbiology 2023Quote: ... 20 μL of lysate was incubated at room temperature with 20 μL of BrightGlo reagent (Promega) for 5 minutes ...
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bioRxiv - Microbiology 2023Quote: ... 2μl of the lysate was subsequently used in a 20μL PCR reaction (Promega, Madison WI, USA) with forward primer 342F (5’-CTA CGG GGG GCA GCA G -3’ ...
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bioRxiv - Immunology 2022Quote: ... Nanoluc Luciferase activity in cell lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with Modulus II Microplate Reader User interface (TURNER BioSystems) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and equal amounts of lysates were used for luciferase reporter assay following the manufacturer’s instructions (Promega).
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bioRxiv - Developmental Biology 2023Quote: ... Luciferase activity in the cell lysates was measured using a Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... NanoLuc luciferase activity in the lysates was measured using the Nano-Glo Luciferase Assay System (Promega) with a Tecan Infinite microplate reader ...
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bioRxiv - Immunology 2023Quote: ... The luciferase activity in cell lysates was measured using the Nano-Glo Luciferase Assay System (Promega) and GloMax system (Turner BioSystems) ...
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bioRxiv - Cell Biology 2023Quote: ... 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega). Translation reaction products from 0.25 μg of plasmid DNA were incubated with GST-labeled proteins on glutathione-Sepharose beads in NETN-E buffer (50 mM Tris ...
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bioRxiv - Bioengineering 2023Quote: ... Luciferase signals from the cell lysate were evaluated using the GloMax 96 Microplate Luminometer (Promega, USA).
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bioRxiv - Immunology 2024Quote: ... Luciferase activity was measured in purified CD8 T cell lysates with the luciferase assay system (Promega). Purified CD8 cells were lysed using cell lysis buffer 1X ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA of all the pOXA-48 bearing strains was isolated using the Wizard genomic DNA purification kit (Promega, WI, USA), and quantified using the QuantiFluor dsDNA system (Promega ...
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bioRxiv - Bioengineering 2021Quote: ... Plasmid DNA input was produced by midi prepping an overnight culture of 200 mL LB with the appropriate strain (Promega, A2492) and cleaned again using PCR cleanup (Promega ...
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bioRxiv - Microbiology 2022Quote: ... 2 × 106 cells of each strain were mixed completely with equal volumes of the BacTiter-Glo reagent (Promega Corporation, Madison, WI), followed by incubation at room temperature for 15 min in the dark ...
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bioRxiv - Microbiology 2023Quote: The cytotoxic activity on Hela cells of each bacterial strain was analyzed using the CellTiter-Blue® Cell Viability Assay (Promega) following manufacturer instructions ...
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bioRxiv - Biophysics 2021Quote: ... and were produced by translation in rabbit reticulocyte lysates using TnT Quick Coupled Transcription/Translation System (Promega) (see Supplementary Table 5 for plasmids) ...
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bioRxiv - Molecular Biology 2020Quote: ... Luciferase activity of the lysates was measured using a Nano-Glo Dual-Luciferase Reporter Assay System (Promega) and SpectraMax Paradigm (Molecular Devices ...
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bioRxiv - Molecular Biology 2021Quote: Messenger RNAs were translated in nuclease-treated rabbit reticulocyte lysate (RRL) or wheat germ (WG) extracts (Promega). Typical reactions were composed of 90% v/v RRL ...
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bioRxiv - Cancer Biology 2019Quote: ... Firefly luciferase activity present in cellular lysates were assayed using the dual luciferase reporter system (Promega Corp.), and light emission was quantified using a luminometer (Junior Berlthold).
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bioRxiv - Genomics 2019Quote: ... We measured the luciferase activity with cell lysates using the Dual-Luciferase® Reporter Assay System (Promega). We normalized Firefly luciferase activity to the Renilla luciferase activity ...
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bioRxiv - Bioengineering 2021Quote: Target proteins were in-vitro expressed using rabbit reticulocyte lysate (TNT-coupled reticulocyte system; Promega; #L4600, #L4610) supplemented with either 35S-methionine/S-L-cysteine mix (PerkinElmer ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1ul of yeast lysate was used as template in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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Antibody potency, effector function and combinations in protection from SARS-CoV-2 infection in vivobioRxiv - Microbiology 2020Quote: ... and nanoluc luciferase activity in cell lysates was measured using the Nano-Glo Luciferase Assay System (Promega). Relative luminescence units obtained were normalized to those derived from cells infected with SARS-CoV-2 MA pseudovirus in the absence of monoclonal antibodies ...