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Citations for Promega :
451 - 500 of 699 citations for Zika Virus Lysate DakArD 41662 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Lysates were used to measure NAD+ and NADH levels using the NAD/NADH-Glo Assay (Promega, G9071) by following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were diluted 4-fold with 100 mM ammonium acetate and digested with sequence-grade trypsin (Promega) overnight at RT at an enzyme:substrate ratio of 50:1 (w/w) ...
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bioRxiv - Microbiology 2020Quote: ... Renilla luciferase activity in the cell lysates was measured using the Rluc system (Promega, Madison, WI, USA) (48).
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bioRxiv - Microbiology 2021Quote: ... Lysates were further diluted with 50 mM ammonium bicarbonate to < 2M urea and digested with trypsin (Promega) overnight at 37 ⁰C ...
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bioRxiv - Microbiology 2021Quote: ... and Firefly and Renilla luciferase activities in the lysates were measured using a dual luciferase kit (Promega).
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were stored at −20°C or used immediately in a Dual-Glo luciferase assay (Promega #E2920) with the SpectraMax ID5 microplate reader ...
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bioRxiv - Immunology 2020Quote: ... and finally the luciferase activity in total cell lysates was measured with Glomax (Promega, Madison, WI, USA).
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bioRxiv - Genomics 2019Quote: ... The luciferase activity of cell lysates was measured using a luciferase assay kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and finally the luciferase activity in total cell lysates was measured with Glomax (Promega, Madison, WI, USA).
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bioRxiv - Biochemistry 2020Quote: ... In vitro translation assays were run on the prepared mRNA templates using Rabbit Reticulocyte Lysate kits (Promega), with translation mixes prepared in triplicate as per manufacturer′s protocols (for S2D ...
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bioRxiv - Microbiology 2020Quote: ... and lysates were read on a Promega GloMax Multi Detection unit using Luciferase Assay Reagent II (Promega) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: ... 1500 μg total protein lysate was 100ul slurry of pre-cleared with Protein G agarose beads (Promega), followed by overnight incubation with AXL antibody (2ug Ab/ 100 ul lysate) ...
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bioRxiv - Immunology 2020Quote: ... Firefly luciferase activities were measured in 10 μl of lysate using 50 μl luciferase assay substrate (Promega). To normalise transfection efficiencies β-Galactosidase levels were measured using the β-Galactosidase Enzyme Assay System (Promega) ...
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bioRxiv - Cell Biology 2022Quote: Constructs for in vitro translations in rabbit reticulocyte lysate were based on the pSP64 vector (Promega, USA). Constructs for in vitro translation in the E ...
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bioRxiv - Genomics 2023Quote: ... The Luciferase assay was performed with 100 µL of the cleared lysate (Steady Glo reagent, Promega, E2510) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The quantification of NADPH was assessed in total cell lysates using the NADP/NADPH Assay Kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell lysates were used to determine luciferase activity with the Dual-Luciferase Reporter Assay System (Promega) and a GloMax 96 Microplate Luminometer (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... The Luciferase assay was performed with 100 µL of the cleared lysate (Steady Glo reagent, Promega, E2510) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: The reaction mixture (10 μl) was prepared with 5 μl of rabbit reticulocyte lysate nuclease-treated (Promega), 2 μl of H2O ...
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bioRxiv - Physiology 2023Quote: ... Cell lysates (20 μL) were assayed for luciferase activity using the dual Luciferase Assay System (Promega, USA), according to manufacture instructions ...
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bioRxiv - Immunology 2023Quote: Proteasome activity was measured in cell lysates using the Proteasome-Glo™ 3 Substrate System (Promega, G8531). Corresponding reagents for testing as chymotrypsin-like ...
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bioRxiv - Immunology 2023Quote: ... and 25 µl cell lysates were mixed with 25 µl Bright-Glo or Nano-Glo (both Promega), and luminescence was measured by a luminometer ...
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bioRxiv - Microbiology 2024Quote: ... the lysates were diluted with 50mM HEPES pH8.0 to <2M urea and digested overnight with trypsin (Promega) at 1:50 (enzyme:protein ...
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bioRxiv - Genomics 2019Quote: ... Chile) and Drosophila americana (strain H5 from Mississipi, United States of America) with the Wizard® Genomic DNA Purification Kit (Promega Corporation). We used the consensus sequences from each satDNA identified by RepeatExplorer/TAREAN for primer’s design ...
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bioRxiv - Synthetic Biology 2021Quote: All plasmids within this study were expressed in BL21(DE3) (ATCC #BAA-1025) or BL21(DE3)pLysS strains (Promega, Cat. No. L1195). All strains were grown in lysogeny broth Miller (10 g/L tryptone ...
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bioRxiv - Molecular Biology 2023Quote: PFT-expressing plasmids were purified from an overnight stationary culture of the corresponding strain by using the Wizard Plus SV Minipreps DNA purification System (Promega, cat#A1460) and transformed into E ...
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bioRxiv - Microbiology 2023Quote: We isolated the genomic DNA of the strains used in this work using the Wizard genomic DNA purification kit (Promega, WI, USA) and quantified using the Qubit 4 fluorometer (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... and lysate was either measured directly for GFP signal or incubated with luciferase assay reagent LAR (Promega, E1500) for luminescence signal ...
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bioRxiv - Immunology 2021Quote: ... lysates were transferred to white plates and luminescence was read in a GloMax® Discover Microplate Reader (Promega). Neutralization activity was determined by comparing the signal from the wells infected with virus lacking antibody to the different sera dilutions ...
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bioRxiv - Developmental Biology 2020Quote: ... After 24 hours luciferase activities were assayed in cell lysates using the Dual-Luciferase Reporter Assay System (Promega) and a multi-label counter (Promega GloMax) ...
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bioRxiv - Microbiology 2020Quote: ... dengue protein NS2B/3/4A constructs were expressed in rabbit reticulocyte lysate (TNT coupled T7, Promega Bio Systems) programmed with 1 µg DNA/50 µL and labeled with 20 µCi of L-[35S]-methionine (EasyTag ...
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bioRxiv - Cancer Biology 2019Quote: ... The lysate was quantified for firefly and Renilla luciferase activity using the Dual-Glo Luciferase assay system (Promega) to determine miR-211-dependent target degradation/transcriptional inhibition ...
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bioRxiv - Biochemistry 2020Quote: ... The diluted lysate was added to 100 µl of pre-equilibrated Magne® HaloTag® Beads (Promega, G7282) and incubated overnight on a rotator at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Apoproteins of PHYA-HA and PHYB-HA were prepared using a TNT T7 Coupled Reticulocyte Lysate system (Promega) as described previously75 ...
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bioRxiv - Microbiology 2021Quote: ... 35S methionine-labeled full-length cGAS and E5 was generated with the TnT Coupled Reticulocyte Lysate System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... and cell lysates were prepared and examined by using the Dual luciferase reporter assay system (Promega, Madison, WI), according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2022Quote: ... the beads with bound recombinant proteins were blocked for 1 h at 4°C using reticulocyte lysate (Promega). Next ...
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bioRxiv - Molecular Biology 2020Quote: In vitro transcription/translation of vvl/dfr vDNA was carried out using TNT coupled reticulo lysate system (Promega) and T3 RNA polymerase (New England Biolabs Inc. ...
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bioRxiv - Molecular Biology 2020Quote: Luciferase activity of cell lysate transfected with pGL3 promoter constructs was detected with the Dual Luciferase Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 200 µl/sample) and split into fractions with or without MNase (Promega, 50U/100 µl of nuclear lysate) and incubated for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Lep proteins were then produced from mRNA by in vitro translation using rabbit reticulocyte lysate (Promega, Madison, WI) supplemented with canine pancreatic rough microsomes (tRNA probes ...
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bioRxiv - Molecular Biology 2019Quote: ... NanoLuc and Firefly activities in cell lysates were measured using Nano-Glo Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s protocols.
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bioRxiv - Microbiology 2020Quote: ... and firefly and renilla luciferase activity in the lysates were measured using Dual-Luciferase Reporter Assay System (Promega) in GloMax 20/20 luminometer (Promega) ...
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bioRxiv - Biochemistry 2019Quote: ... Luciferase activity was measured 48 h post-transfection in cell lysates with the Dual Luciferase assay kit (Promega) and a Pherastar FS platereader (BMG Labtech) ...
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bioRxiv - Microbiology 2021Quote: ... 40 μL of the lysate supernatant were mixed with the same volume of Nano-Glo reaction solution (Promega), incubated for 3 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... The preparation of cell lysates and dual luciferase assay were carried out according to the manufacturer’s protocol (Promega). After 24 h ...
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bioRxiv - Molecular Biology 2020Quote: β-globin 48S IC and H4 48S IC were isolated from nuclease-treated rabbit reticulocyte lysate (RRL) (Promega L4960), as previously described (Simonetti et al. ...
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bioRxiv - Microbiology 2021Quote: In vitro transcription and translation reactions were conducted using the TNT coupled rabbit reticulocyte lysate system (Promega, L4610) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Luciferase activity of cell lysate transfected with pGL3 promoter constructs was detected with the Dual Luciferase Assay (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Luciferase and β-Gal activity were evaluated in neuronal cell lysates using commercial assay kits (Promega, #E2000, #1500). Luminescence recordings were performed using an Infinite M200 microplate reader with an injector system (Tecan) ...