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Citations for Promega :
251 - 300 of 699 citations for Zika Virus Lysate DakArD 41662 strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were used for luciferase assays (20 µL) with Luciferase assay system (Promega) and RNA extraction (20 µL ...
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bioRxiv - Biophysics 2023Quote: ... Luciferase activity of lysates was measured using the Dual Luciferase Assay Reagents (Promega) on a GloMax Multi microplate luminometer (Promega) ...
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bioRxiv - Immunology 2024Quote: ... Luciferase activity was measured in cell lysates collected 8 hours after transfection (Promega). To correct for transfection efficiency ...
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bioRxiv - Immunology 2023Quote: ... The luminescence of lysates was detected by Luciferase Assay System (Promega, Cat# E1500) and analyzed using a plate spectrophotometer ...
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bioRxiv - Molecular Biology 2024Quote: In vitro translation was performed using a rabbit reticulocyte lysate system (Promega, L4960). Capped and polyadenylated reporter mRNAs containing the first 135 nucleotides of NPR coding sequences (human ...
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bioRxiv - Molecular Biology 2024Quote: Rluc mRNA and Fluc mRNA were incubated with rabbit reticulocyte lysate (RRL, Promega) to generate the mRNA-ribosome complex ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA from Xf strain CFBP 8070 was extracted with the Wizard genomic DNA Purification Kit (Promega, France) and used to prepare a 10-fold serial dilutions from 220 ng.mL−1 (corresponding to 0.8×108 copies.mL−1 of genomic DNA ...
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bioRxiv - Microbiology 2020Quote: ... Genomic DNA was isolated from strains containing extragenic suppressor mutations using the Wizard Genomic DNA Purification Kit (Promega) and sent for whole genome sequencing (Tufts University School of Medicine Genomics Core).
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bioRxiv - Molecular Biology 2019Quote: ... We pooled the BKE strains and prepared a genomic DNA mixture using Wizard genome DNA purification kit (Promega). We used this DNA preparation to transform an smpB-deleted strain of B ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA from nourseothricin resistant strains was extracted using the Wizard Genomic DNA Purification Kit (Promega, Madison, WI) from 3 mL of overnight culture in CAMHB containing NAT [32 µg/mL ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was extracted from the grown strain using the Wizard® Genomic DNA Purification Kit (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... The chromosomal DNA of all strains was extracted using the Wizard® Genomic DNA Purification Kit (Promega, USA) and the integration of antibiotic resistance cassettes or reporter genetic constructs in the genome was confirmed by PCR under standard conditions using the GoTaq Green Master Mix (Promega ...
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bioRxiv - Synthetic Biology 2023Quote: ... strain ADP were extracted using Wizard™ Genomic DNA Purification Kits following the standard protocol provided by Promega.
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bioRxiv - Physiology 2020Quote: ... oligo-dT primed cDNA was synthesized from 500 ng of total RNA using Murine Moloney Leukaemia Virus reverse transcriptase (Promega, USA). qRT-PCR was performed using a ViiA Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... the cell viability of each infected virus variants was measured using CellTiter 96 Aqueous One solution Cell Proliferation Assay (Promega, USA). The relative cell viability was calculated by normalizing the absorbance value of treated virus samples against untreated virus samples ...
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bioRxiv - Microbiology 2019Quote: ... 4 × Promega Wizard® Minicolumns per replicate with a ratio of 0.5:1 virus concentrate to Wizard® PCR Preps DNA Purification Resin (Promega: A7170). The resultant extract was found to be inhibitory to all enzymatic reactions ...
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bioRxiv - Cell Biology 2021Quote: ... The primary MEFs were immortalised by transfection of 2 μg of simian virus 40 large T-antigen-expressing vector employing Fugene 6 Transfection Reagent (Promega, E2311) followed by five rounds of a 1 in10 split to achieve 1/100,000-fold splitting ...
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bioRxiv - Microbiology 2022Quote: ... We performed duplicate serial dilutions using supernatants collected from the virus rescues and measured luciferase expression at each dilution using Bright-Glo Luciferase Assay System (Promega, E2610). Virus titers were calculated as relative light units (RLU ...
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bioRxiv - Molecular Biology 2023Quote: ... Produced pseudoviruses were titrated on HEK-293T-ACE2 by performing duplicate serial dilutions and virus titers were measured 48 hours after infection using Bright-Glo Luciferase Assay System (Promega, E2610).
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bioRxiv - Molecular Biology 2023Quote: ... Cells were incubated at 37 °C for two days and the nLuc activity from the virus replication was measured by using Nano-Glo® Luciferase Assay System (Promega) following the manufacturer’s protocol using a plate reader (HT4 Biotek) ...
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bioRxiv - Immunology 2024Quote: ... The virus neutralization titers were determined by measuring the NLuc activity using the Nano-Glo Luciferase Assay System (Promega, Madison, WI) following the manufacturer’s conditions ...
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bioRxiv - Immunology 2021Quote: ... to each cell lysate and measuring the luminescence using a GloMax portable luminometer (Promega).
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bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
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bioRxiv - Biochemistry 2021Quote: ... Cell lysates were analyzed by Western blot while Renilla luciferase activity (Glomax luminometer, Promega) was used to correct for transfection efficiency.
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bioRxiv - Biophysics 2020Quote: ... In vitro translation of the mRNA was carried out using rabbit reticulocyte lysate (Promega) supplemented with canine rough microsomes (tRNA probes ...
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bioRxiv - Microbiology 2021Quote: ... Luciferase expression in the cell lysates was determined using a dual-luciferase kit (Promega) according to the manufacturer’s guidelines ...
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bioRxiv - Pathology 2021Quote: ... Fluorescence of the lysates was then analyzed using a multi detection plate reader (Promega Glomax Multi Detection System ...
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bioRxiv - Cell Biology 2022Quote: ... In vitro translation was done using the TNT T7 Reticolucyte Lysate System (Promega Corp.), in the presence of radioactive 35S-methionine ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were harvested 24 h after infection in Reporter Lysis Buffer (Promega, E397A), lysed by freeze thawing at -80°C ...
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bioRxiv - Microbiology 2020Quote: ... Half the lysate was used to quantify luciferase protein (Promega Luciferase Assay System #E1501) using a Spectramax L luminometer with reagent injectors in technical triplicate ...
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bioRxiv - Cancer Biology 2021Quote: ... and combining equal volumes of cell lysates with the Bright-Glo™ Reagent (Promega). Co-transfection with a CMV-beta-galactosidase plasmid and bicinchoninic acid protein quantification assays were used for luciferase signal normalization.
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bioRxiv - Microbiology 2020Quote: ... Lysates were assayed for luciferase activity using the Dual Luciferase Reporter Assay System (Promega) and a luminometer with dual injectors ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were assayed for luciferase activity using the Dual Luciferase Reporter Assay System (Promega) and a luminometer with dual injectors ...
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bioRxiv - Biochemistry 2020Quote: ... and the pre-cleared lysate was supplemented with 2 µl of RNasin Plus (Promega), added onto the freshly prepared ...
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bioRxiv - Immunology 2022Quote: ... cell lysates were prepared and analyzed using the Dual-Luciferase Report Assay System (Promega).
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bioRxiv - Plant Biology 2022Quote: ... Cells were collected and lysate in BugBuster (EMDMillipore) with 5 ul RQ1 DNase (Promega). The supernatant was flowed through a column packed with Ni-NTA (QIAGEN) ...
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bioRxiv - Molecular Biology 2019Quote: ... whereas Control Firefly mRNA was provided with Rabbit Reticulocyte Lysate System (Promega Cat#L4960). In vitro reaction was translated for 15 minutes at 37°C and stopped with the addition of 100 µg of cycloheximide and stored on ice prior to luciferase assay.
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bioRxiv - Developmental Biology 2019Quote: ... we produced recombinant H3 mutant proteins from mRNAs using rabbit reticulocyte lysate (Promega L4600). After 3h of incubation at 4°C in interphase extracts followed by another 3h incubation with anti-HA beads ...
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bioRxiv - Microbiology 2019Quote: ... ATP was estimated from bacterial lysates using Bac Titer-GloTM Assay kit from Promega using manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Cell lysates (20μl) were mixed with 100 μl Renilla Luciferase Assay System Substrate (Promega). The luminescence level was measured and the relative light units (RLUs ...
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bioRxiv - Microbiology 2021Quote: ... 20 μL of the lysate and 10 μL of the luciferase substrate (Promega, E4550) were added into white 96-well plates for chemiluminescence detection using a Varioskan LUX Multimode Microplate Reader (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... 20 μL of the lysate and 10 μL of the luciferase substrate (Promega, E4550) were added into white 96-well plates for chemiluminescence detection using a Varioskan LUX Multimode Microplate Reader (ThermoFisher ...
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bioRxiv - Neuroscience 2021Quote: ... and 25 uL of lysate was mixed with ONE-Glo luciferase assay buffer (Promega) in opaque 96-well plates ...
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bioRxiv - Molecular Biology 2021Quote: In vitro translation reactions were performed using nuclease-treated rabbit reticulocyte lysate (RRL) (Promega) following the manufacturer’s protocol for non-radioactive luciferase reactions ...
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bioRxiv - Immunology 2021Quote: ... 20 μL lysate was added to 100 μL Luc substrate (Promega Luciferase Assay System). The amount of luciferase was quantified by luminescence (relative luminescence units (RLU)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lysates were obtained using lysis buffer provided in Dual Luciferase Assay Kit (Promega). Firefly and Renilla luciferase readings were recorded 48 hours posttransfection using Luminometer (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: Fifty μl protein expression reaction using TNT SP6 Coupled Reticulocyte Lysate System (Promega, L4600) containing 1000 ng pIX-Halo-bZIP plasmids was incubated for 3 h at 30°C ...
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bioRxiv - Cell Biology 2023Quote: ... using the rabbit reticulocytes lysates Nuclease-treated kit and the transcendTM tRNA from Promega, as per the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were examined for luciferase activity using a commercial kit assay (Promega Cat.# E2810). Lysates (20 µL ...
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bioRxiv - Microbiology 2023Quote: ... and 50 µl cleared lysate was mixed with 50 µl Bright Glo reagent (Promega) for determination of viral RNA synthesis/protein expression or 50 µl CellTiter-Glo reagent (Promega ...