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Citations for Promega :
401 - 450 of 1997 citations for Human TGF beta 3 TGFB3 qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... qPCR reactions were set up as follows: 6.25 µl of GoTaq® qPCR Master Mix (Promega, Madison, WI, USA), 4.25 µl of nuclease-free water ...
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bioRxiv - Molecular Biology 2023Quote: ... a qPCR was performed using 40 ng digested genomic DNA as template with the GoTaq qPCR master mix (Promega) according to manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2023Quote: ... with 1 ug of RNA and RT-qPCR reactions were performed using GoTaq® qPCR Master Mix (A6002 - Promega). Analyses were carried out in the QuantStudio™ 5 Real-Time PCR System ...
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bioRxiv - Plant Biology 2023Quote: ... Two µl of cDNA (1:4 dilution) was added to a 20µl qPCR reaction using the GoTaq qPCR Master Mix (Promega) and ran on a BioRad CFX Opus96 thermocycler ...
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bioRxiv - Plant Biology 2024Quote: ... The cDNA mixture was used for qRT-PCR using GoTaq® qPCR and RT-qPCR Systems (Promega, WI, USA) on a 7300 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Physiology 2024Quote: ... The luciferase activity was measured by Luc-Pair™ Duo-Luciferase HS Assay Kit (cat. no. LF600, Promega, USA) and normalized to the TK reporter signal.
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bioRxiv - Molecular Biology 2021Quote: ... reverse transcription assays were performed according to the manufacturer’s instructions with 2,5 μg of total RNA in the presence of 5 μM of oligo(dT) primer (Table S3) and 25 ng/μl of Random Primers (Promega Cat#C118A) using the SuperScript™ IV Reverse Transcriptase (Invitrogen Cat#18090010) ...
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bioRxiv - Genetics 2021Quote: ... Colonies were genotyped using the T7/SP6 primers (Promega). Plasmids from the validated colonies were isolated using the QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Genomics 2021Quote: ... and Oligo (dT) 15 primer (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... and Oligo (dT) 15 primer (Promega, Madison, WI, USA). STEP ONE PLUS Real Time PCR system (Applied Biosystems Japan ...
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bioRxiv - Plant Biology 2020Quote: ... the cDNA was synthesized using oligo(dT) primer (Promega) with 18S rRNA specific primer and ReverTraAce reverse transcriptase (Toyobo) ...
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bioRxiv - Bioengineering 2020Quote: ... 1 µl random primers (50 ng·µl−1, Promega, C1181), 2 µL 0.1M DTT ...
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bioRxiv - Microbiology 2021Quote: ... according to the manufacturers’ protocol with random primers (Promega). Two microliters of diluted cDNA were amplified with SYBR™ Green PCR Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... random primers and RNasin® ribonuclease inhibitor (Promega N2511). All these steps were carried out following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis was performed using random primers (Promega, C1181) and M-MLV Reverse Transcriptase RNase H (Solis BioDyne ...
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bioRxiv - Genetics 2020Quote: ... with both random hexamer and oligo(dT) primers (Promega) after the DNase (Promega ...
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bioRxiv - Immunology 2021Quote: ... 1µl of random hexamer primers (Promega, Catalogue no.-C118A) were added to the RNA ...
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bioRxiv - Microbiology 2023Quote: ... using M-MLV-RT and random hexamer primers (Promega) as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... RT-PCR with random primers (Promega, Madison, WI, USA) and Moloney murine leukemia virus M-MLV reverse transcriptase (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... RT-PCR with random primers (Promega, Madison, WI, USA) and Moloney murine leukemia virus MMLV reverse transcriptase (Promega ...
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bioRxiv - Microbiology 2023Quote: ... using M-MLV-RT and random hexamer primers (Promega) as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... oligo(dT) primer and reverse transcriptase ImProm-II (Promega). Two-step quantitative real-time PCR(qPCR ...
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bioRxiv - Bioengineering 2023Quote: ... 1 μL random primers (50 ng/μL, Promega, C1181), 2 μL 0.1 M DTT ...
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bioRxiv - Cell Biology 2024Quote: ... random primers (10 ng/μl, C1181, Promega, Wisconsin, USA) and dNTPs (0.5 mM ...
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bioRxiv - Microbiology 2023Quote: ... 5’-CGCCTTCGTTACAAGCATCG-3’; antisense, 5’-AAGAGCAAACGCATCTCCGA-3’), GAPDH (sense, 5’-ACCCACTCCTCCACCTTTGAC-3’; antisense, 5’-CTGTTGCTGTAGCCAAATTCGT-3’) using Green GoTaq master mix (Promega, Madison, WI) with C1000 Touch Thermal Cycler (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... At the indicated time points growth media was removed and 10 μl Beta-Glo (Promega, Madison, Wisconsin) was added per well ...
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bioRxiv - Molecular Biology 2020Quote: ... The synthesized cDNA was served as the template for qPCR and the GoTaq® qPCR Master Mix (A6001, Promega, USA) was used ...
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bioRxiv - Plant Biology 2020Quote: ... The concentrations of the resulting cDNAs were adjusted to 10 ng/µL and 2 µL were used for RT-qPCR reactions performed with the GoTaq qPCR Master Mix (Promega) in a final volume of 10 µL ...
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bioRxiv - Microbiology 2020Quote: RT-qPCR analyses of NoV GII were carried out using GoTaq® Probe 1-Step RT-qPCR System (Promega, USA). Primers and the FAM/TAMRA-labelled probe of NoV GII were according to ISO 15216-1:2017 ...
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bioRxiv - Microbiology 2022Quote: RT-qPCR analyses of NoV GII were carried out using GoTaq® Probe 1-Step RT-qPCR System (Promega, USA). Primers and the FAM/TAMRA-labelled probe of hNoV GII were according to ISO 15216-1:2017 ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was completed using Applied Biosystems QuantStudio3 Real-Time PCR system using GoTaq qPCR Master Mix with SYBR Green (Promega). All primers used for RT-qPCR are shown in Table S3 ...
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bioRxiv - Neuroscience 2023Quote: ... 5-30 ng of the original RNA was used to perform qPCR for Dkk3 and Dkk1 using GoTaq qPCR Master Mix (Promega) in a CFX96 Bio-rad system following the manufacturer’s protocol (2 min at 95°C followed by 40 cycles of denaturing at 95°C and annealing/extension at 60°C) ...
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bioRxiv - Microbiology 2024Quote: Plasmids used as standard to normalize the qPCR data were obtained by blunt end ligation of the flavivirus qPCR products into pGEM-3Z (Promega), using SmaI (NEB ...
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bioRxiv - Genomics 2024Quote: ... Each qPCR reaction was composed of 12.5 µL of 2x GoTaq qPCR Probe Master Mix (Promega Corporation, Madison, WI, USA), 2.5 µL of each primer (0.5 µM) ...
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bioRxiv - Immunology 2021Quote: ... using the GoTaq qPCR Master Mix kit (Promega). Gene expression was normalized to the housekeeping gene Rplp0 and expressed as fold change compared to CD11cWT samples ...
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bioRxiv - Molecular Biology 2021Quote: ... Either GoTaq 1-Step RT-qPCR System (Promega) was used or reverse transcription for mRNAs was performed using Superscript IV RT (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2021Quote: ... with GoTaq qPCR Master Mix (Promega, Madison, WI). The fold change or percentage input of the samples was calculated using the QuantStudio™ Design & Analysis Software Version 1.2 (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... using GoTaq qPCR Master Mix (Promega, Southampton, UK). Relative gene expression was calculated using the comparative Ct method (2−δδCt)[47] ...
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bioRxiv - Plant Biology 2021Quote: ... using the GoTaq qPCR Mastermix (Promega, Walldorf, Germany) and cDNAs synthesized from DNase- treated total RNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... containing 1X GoTaq qPCR Master Mix (Promega Corporation), 300 nM CXR Reference Dye and final concentration 200nM of each SYBR green designed primers described in Supplementary Table 2 ...
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bioRxiv - Microbiology 2021Quote: ... The GoTaq probe qPCR Master Mix (Promega, A6102) and GoTaq G2 (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... containing 1X GoTaq qPCR Master Mix (Promega Corporation), 300 nM CXR Reference Dye ...
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bioRxiv - Molecular Biology 2022Quote: ... or the GoTaq® qPCR kit (Promega, A6001) was used ...
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bioRxiv - Physiology 2022Quote: ... with GoTaq® qPCR Master Mix (Promega, USA) and acquired with Step One software v.2.3 ...
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bioRxiv - Neuroscience 2021Quote: ... containing 1X GoTaq qPCR Master Mix (Promega Corporation), 300 nM CXR Reference Dye ...
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bioRxiv - Physiology 2020Quote: ... qPCR was performed using GoTaq Master Mix (Promega) and a 96 well thermal cycler (Applied Biosystems) ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... SYBR Green GoTaq qPCR Master Mix (Promega, A6002) and primers listed in the Table number 1 at a final concentration of 300 nM ...
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bioRxiv - Microbiology 2022Quote: ... and 1X GoTaq qPCR Master Mix (Promega, A6002). Amplifications were performed using a CFX Connect Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Physiology 2020Quote: ... GoTaq® qPCR MasterMix (Promega, Madison, WI, USA) and 3ng of DNA sample were combined to amplify both mtDNA and nuclear DNA by 7900HT RT-PCR system (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... using GoTaq Probe qPCR Master Mix (Promega, USA).